Effects of global warming on mosquitoes & mosquito‐borne diseases and the new strategies for mosquito control

Global warming has shortened mosquitoes’ lifecycle period and increased the disease transmission rates by mosquito vectors. We reviewed only three mosquito‐borne diseases: malaria, dengue fever, and the Japanese encephalitis. Billions of people get infected with those diseases and millions of people die every year. Although we struggle to find the most effective way to control mosquitoes using various methods (including pesticides), mosquito‐borne diseases are still among the most serious problems being faced. This paper, therefore, reviews the strategies for controlling mosquitoes. The use of pesticides to control mosquitoes might have more negative effects on humans and environments than benefits. Although the development of genetically modified (GM) mosquitoes raises new hopes for effective mosquito control, it will take longer to assess the risks to humans and environments. Furthermore, there has been concern about the possible adverse effects from the release of GM mosquitoes into the environment. The various mosquito traps may not be as effective at controlling only female mosquito populations. Therefore, new strategies for the control of mosquitoes are vital. The smart mosquito counter device was developed by Korean Centre for Disease Control (KCDC) in 2013. The mosquito pest control office is able to set up the appropriate mosquito control strategies by using quantitative mosquito information. The smart device will bring mosquito control in line with modern smart generation technology and the device will also soon be able to identify different mosquito species. This new strategy will change the methods of mosquito control and will provide beneficial effects toward sustainable nature and human health.     

Linking mosquito surveillance to dengue fever through Bayesian mechanistic modeling

Our ability to effectively prevent the transmission of the dengue virus through targeted control of its vector, Aedes aegypti, depends critically on our understanding of the link between mosquito abundance and human disease risk. Mosquito and clinical surveillance data are widely collected, but linking them requires a modeling framework that accounts for the complex non-linear mechanisms involved in transmission. Most critical are the bottleneck in transmission imposed by mosquito lifespan relative to the virus’ extrinsic incubation period, and the dynamics of human immunity. We developed a differential equation model of dengue transmission and embedded it in a Bayesian hierarchical framework that allowed us to estimate latent time series of mosquito demographic rates from mosquito trap counts and dengue case reports from the city of Vitória, Brazil. We used the fitted model to explore how the timing of a pulse of adult mosquito control influences its effect on the human disease burden in the following year. We found that control was generally more effective when implemented in periods of relatively low mosquito mortality (when mosquito abundance was also generally low). In particular, control implemented in early September (week 34 of the year) produced the largest reduction in predicted human case reports over the following year. This highlights the potential long-term utility of broad, off-peak-season mosquito control in addition to existing, locally targeted within-season efforts. Further, uncertainty in the effectiveness of control interventions was driven largely by posterior variation in the average mosquito mortality rate (closely tied to total mosquito abundance) with lower mosquito mortality generating systems more vulnerable to control. Broadly, these correlations suggest that mosquito control is most effective in situations in which transmission is already limited by mosquito abundance.     

Mosquito allergy and mosquito salivary allergens

Allergic reactions to mosquito bites are caused by allergens in mosquito saliva. In this review, allergic reactions to mosquito salivary allergens, and characteristics of salivary allergens and their recombinant forms are described. The use of the recombinant allergens in the diagnosis of mosquito allergy is discussed.     

Mosquito transposable elements

The completion of the genome assembly for the African malaria mosquito, Anopheles gambiae, and continuing genomic efforts for the yellow fever mosquito, Aedes aegypti, have allowed the use of bioinformatics tools to identify and characterize a diverse array of transposable elements (TEs) in these and other mosquito genomes. An overview of the types and number of both RNA-mediated and DNA-mediated TEs that are found in mosquito genomes is presented. A number of novel and interesting TEs from these species are discussed in more detail. These findings have significant implications for our understanding of mosquito genome evolution and for future modifications of natural mosquito populations through the use of TE-mediated genetic transformation.     

Larval mosquito control through deployment of xiphidiocercariae

Larval trematodes (xiphidiocercariae) have been found to encyst in mosquito larvae. When the number of cysts is high, this results in the death of the mosquito larva. It is suggested that populating waters containing mosquito larvae with xiphidiocercariae can be developed as a mosquito control measure.     

Mathematical modelling of mosquito dispersal in a heterogeneous environment

Mosquito dispersal is a key behavioural factor that affects the persistence and resurgence of several vector-borne diseases. Spatial heterogeneity of mosquito resources, such as hosts and breeding sites, affects mosquito dispersal behaviour and consequently affects mosquito population structures, human exposure to vectors, and the ability to control disease transmission. In this paper, we develop and simulate a discrete-space continuous-time mathematical model to investigate the impact of dispersal and heterogeneous distribution of resources on the distribution and dynamics of mosquito populations. We build an ordinary differential equation model of the mosquito life cycle and replicate it across a hexagonal grid (multi-patch system) that represents two-dimensional space. We use the model to estimate mosquito dispersal distances and to evaluate the effect of spatial repellents as a vector control strategy. We find evidence of association between heterogeneity, dispersal, spatial distribution of resources, and mosquito population dynamics. Random distribution of repellents reduces the distance moved by mosquitoes, offering a promising strategy for disease control.     

Combining GIS application and climatic factors for mosquito control in Eastern Province,Saudi Arabia

This study aims to determine the effect of climatic factors (temperature, relative humidity and rainfall) on mosquito abundance and to map mosquito larva breeding sites using GIS application in Eastern Province, Saudi Arabia. The data pertaining to larval and adult mosquito abundance/distribution and climatic factors were collected for the study period of 2014. Bi-variate and multivariate analyses were performed to determine the relationship between mosquito abundance and climatic factors (temperature, relative humidity and rainfall). The utilization of GIS with GPS facilitates to identify and map larva breeding sites in the study area. The result showed strong negative correlation between mosquito abundance and temperature while there appeared a strong positive correlation with relative humidity and moderate positive correlation with rainfall. Low mosquito abundance was observed at high temperatures whereas high and moderate mosquito abundance was observed at high humidity and during rainy months, respectively. In the adult mosquito, the regression model for three climatic factors (temperature, RH and rainfall) and other factors showed a variation of 84.5% of the variance accounted for the climatic factors while the remaining 15.5% were attributed to other factors. In larva, 64.3% of the variance accounted for climatic factors, and the remaining 35.7% attributed to other factors such as the presence of vegetation, waste materials and water reservoirs such as ditches. In comparison, the larva seems influenced by the presence of vegetation, waste material, water reservoirs and ditches and less influenced by climatic factors than the adult mosquito. Development of a risk map by considering the flying distance of the adult mosquito from the studied sites showed three major clusters where a recommendation for management control program was suggested.     

驱蚊、灭蚊植物资源及其应用前景分析

蚊虫吸食人血、传播疾病,严重危害人类健康。长期以来,化学合成杀虫剂由于驱灭蚊虫的效果好、持续时间长,一直占据主要地位,但长期使用化学合成杀虫剂,不仅可使蚊虫产生抗药性,而且还会造成环境污染,因此环境友好型的蚊虫防治手段日益受到人们的关注。该论文基于蚊虫与水、植物等环境因素关系的分析,阐述了植物在防治蚊虫方面的潜力;概括了我国的驱蚊、灭蚊植物资源及开发利用现状;探讨了驱蚊、灭蚊植物在防治蚊虫产品开发、生态保健型园林绿地和健康湿地系统建设等方面应用情况并展望了驱蚊、灭蚊植物资源开发利用的广阔前景,以期为蚊虫的生态防治提供新思路。     

河蟹、克氏原螯虾、黄鳝摄食生态的研究

通过疫区围网养殖河蟹、黄鳝、克氏原螯虾试验,解剖观察食性表明:河蟹、黄鳝、克氏原螯虾都可以摄食钉螺、蚊幼虫.为进一步验证它们的这一食性及其摄食量,通过模拟网栏养殖模式的数据统计表明:河蟹每天每只能摄食蚊幼虫2.4尾、钉螺4.8个;克氏原螯虾每天每只能摄食蚊幼虫17.尾、钉螺0.9个;黄鳝每天每尾摄食蚊幼虫8.1尾、钉螺0.7个;河蟹、克氏原螯虾、黄鳝摄食生态的研究为生物灭螺、灭蚊提供新的途径.       

The Temporal Spectrum of Adult Mosquito Population Fluctuations: Conceptual and Modeling Implications

An improved understanding of mosquito population dynamics under natural environmental forcing requires adequate field observations spanning the full range of temporal scales over which mosquito abundance fluctuates in natural conditions. Here we analyze a 9-year daily time series of uninterrupted observations of adult mosquito abundance for multiple mosquito species in North Carolina to identify characteristic scales of temporal variability, the processes generating them, and the representativeness of observations at different sampling resolutions. We focus in particular on Aedes vexans and Culiseta melanura and, using a combination of spectral analysis and modeling, we find significant population fluctuations with characteristic periodicity between 2 days and several years. Population dynamical modelling suggests that the observed fast fluctuations scales (2 days-weeks) are importantly affected by a varying mosquito activity in response to rapid changes in meteorological conditions, a process neglected in most representations of mosquito population dynamics. We further suggest that the range of time scales over which adult mosquito population variability takes place can be divided into three main parts. At small time scales (indicatively 2 days-1 month) observed population fluctuations are mainly driven by behavioral responses to rapid changes in weather conditions. At intermediate scales (1 to several month) environmentally-forced fluctuations in generation times, mortality rates, and density dependence determine the population characteristic response times. At longer scales (annual to multi-annual) mosquito populations follow seasonal and inter-annual environmental changes. We conclude that observations of adult mosquito populations should be based on a sub-weekly sampling frequency and that predictive models of mosquito abundance must include behavioral dynamics to separate the effects of a varying mosquito activity from actual changes in the abundance of the underlying population.     

Temporal abundance of Aedes aegypti in Manaus,Brazil, measured by two trap types for adult mosquitoes

A longitudinal study was conducted in Manaus, Brazil, to monitor changes of adult Aedes aegypti (L.) abundance. The objectives were to compare mosquito collections of two trap types, to characterise temporal changes of the mosquito population, to investigate the influence of meteorological variables on mosquito collections and to analyse the association between mosquito collections and dengue incidence. Mosquito monitoring was performed fortnightly using MosquiTRAPs (MQT) and BG-Sentinel (BGS) traps between December 2008-June 2010. The two traps revealed opposing temporal infestation patterns, with highest mosquito collections of MQTs during the dry season and highest collections of BGS during the rainy seasons. Several meteorological variables were significant predictors of mosquito collections in the BGS. The best predictor was the relative humidity, lagged two weeks (in a positive relationship). For MQT, only the number of rainy days in the previous week was significant (in a negative relationship). The correlation between monthly dengue incidence and mosquito abundance in BGS and MQT was moderately positive and negative, respectively. Catches of BGS traps reflected better the dynamic of dengue incidence. The findings help to understand the effects of meteorological variables on mosquito infestation indices of two different traps for adult dengue vectors in Manaus.     

Deep sequencing reveals extensive variation in the gut microbiota of wild mosquitoes from Kenya

The mosquito midgut is a hostile environment that vector‐borne parasites must survive to be transmitted. Commensal bacteria in the midgut can reduce the ability of mosquitoes to transmit disease, either by having direct anti‐parasite effects or by stimulating basal immune responses of the insect host. As different bacteria have different effects on parasite development, the composition of the bacterial community in the mosquito gut is likely to affect the probability of disease transmission. We investigated the diversity of mosquito gut bacteria in the field using 454 pyrosequencing of 16S rRNA to build up a comprehensive picture of the diversity of gut bacteria in eight mosquito species in this population. We found that mosquito gut typically has a very simple gut microbiota that is dominated by a single bacterial taxon. Although different mosquito species share remarkably similar gut bacteria, individuals in a population are extremely variable and can have little overlap in the bacterial taxa present in their guts. This may be an important factor in causing differences in disease transmission rates within mosquito populations.     

The association between meteorological variables and the abundance of Aedes taeniorhynchus in the Florida Keys

The black salt marsh mosquito, Aedes taeniorhynchus, is a serious nuisance pest and a potential vector of a number of arboviruses. This study examined the effect of wind direction, wind speed, temperature, and time of year on the abundance of Ae. taeniorhynchus collected in CO₂‐baited light traps at 12 sites in the Florida Keys during 2004. The dependent variable analyzed was the natural log of weekly mosquito abundance. The previous week's wind speed and wind direction, and the current week's temperature were used as independent variables. Simple and multiple linear regression models were used to assess the significance and nature of association between the meteorological variables and the natural log of mosquito abundance, and to determine whether the meteorological variables had significant associations with mosquito abundance after also controlling for time of year. Week of year was treated as a circular independent variable in the regression models, using the sine and cosine of week in radians to model the periodic seasonal fluctuation in mosquito abundance. Mosquito abundance was significantly associated with all meteorological variables and with week of year. Individually, previous week's wind speed and wind direction, and current week's temperature were able to explain respectively 24.5%, 24.5%, and 52.1% of the variation in mosquito abundance observed over the year. Week of year had the strongest individual association with mosquito abundance, explaining 65.7% of the variation in mosquito abundance. The meteorological variables were still significantly associated with mosquito abundance, after controlling for week of year. Week and the meteorological variables together explained 79.2% of the variation in mosquito abundance. The regression models fit to the data from this study suggest a strong periodic seasonal variation in mosquito abundance, with meteorological conditions explaining a significant portion of the variation beyond the seasonal trend.     

Effects of flash flooding on mosquito and community dynamics in experimental pools

Pulsed disturbances of larval mosquito sites are likely to have a direct negative effect on mosquitoes but may also have indirect effects due to the alteration of community structure. These altered communities may become attractive to gravid mosquitoes searching for oviposition sites when the disturbances decrease the abundance of mosquito antagonists such as competitors, which often results in an increase in mosquito food resources. However, flash flood disturbances in intermittent riverbeds can also remove mosquito food resources such as algae, so that the net effect of flash floods could be either to increase or decrease mosquito abundance. We conducted an outdoor mesocosm experiment to assess the effects of flash floods on mosquito oviposition habitat selection and larval abundance during the post‐disturbance period of community recovery. Mesocosms were artificially flooded. Mosquito oviposition, immature abundance, invertebrate species diversity, chlorophyll a, and abiotic parameters were monitored. Our results showed that the flash flood negatively affected phytoplankton and zooplankton, leading to a decrease of mosquito oviposition in flooded mesocosms compared to non‐flooded mesocosms. More broadly, this study indicates how disturbances influence mosquito oviposition habitat selection due to the loss of food resources in ephemeral pools, and it highlights the importance of considering the effects of disturbances in management, habitat restoration, and biodiversity conservation in temporary aquatic habitats.     

Mosquito/microbiota interactions: from complex relationships to biotechnological perspectives

To date around 3500 different species of mosquito have been described, several tens of which are vectors of pathogens of remarkable interest in public health. Mosquitoes are present all around the world showing a great ability to adapt to very different types of habitats where they play relevant ecological roles. It is very likely that components of the mosquito microbiota have given the mosquito a great capacity to adapt to different environments. Current advances in understanding the mosquito-microbiota relationships may have a great impact in a better understanding of some traits of mosquito biology and in the development of innovative mosquito-borne disease-control strategies aimed to reduce mosquito vectorial capacity and/or inhibiting pathogen transmission.     

Biochemical analysis of a blood meal-induced Aedes aegypti glutamine synthetase gene

Glutamine synthetase (GS) in the mosquito, Aedes aegypti, is induced in the midgut following a blood meal. Mosquito GS message is detected as soon as 1 h post-blood feeding and remains stable for 18 h. Using a PCR product encoding mosquito GS, a λgt10 adult female mosquito cDNA library was screened. A cDNA clone, pCl5A2, encoding the full translation product of mosquito GS was isolated and sequence analyses performed. Mosquito GS cDNA is 2.5 kb in length and its putative translation product shares all the conserved regions characteristic of the GS gene family, including the presumed ATP biding site. Glutamine synthetase activity in the mosquito midgut is highest at 18 h post-blood feeding. Activity can be detected over a broad pH range, from 6.0 to 7.5. Unlike other cellular GS enzymes, mosquito GS is not active in the presence of ATP. Very low dosages (0.05 mM) of L-methionine S-sulfoximine are sufficient to partially inhibit mosquito GS activity. Inhibition of GS disrupts the normal formation of the midgut peritrophic matrix, suggesting that GS enzyme might be involved in the initial pathway of chitin synthesis. The unique expression pattern and inducible nature of the mosquito GS gene make it an interesting candidate for studying promoter function. Additionally, the blood meal activation of the GS gene makes this a potentially valuable tool in mosquito transformation studies.     

Modeled response of the West Nile virus vector Culex quinquefasciatus to changing climate using the dynamic mosquito simulation model

Climate can strongly influence the population dynamics of disease vectors and is consequently a key component of disease ecology. Future climate change and variability may alter the location and seasonality of many disease vectors, possibly increasing the risk of disease transmission to humans. The mosquito species Culex quinquefasciatus is a concern across the southern United States because of its role as a West Nile virus vector and its affinity for urban environments. Using established relationships between atmospheric variables (temperature and precipitation) and mosquito development, we have created the Dynamic Mosquito Simulation Model (DyMSiM) to simulate Cx. quinquefasciatus population dynamics. The model is driven with climate data and validated against mosquito count data from Pasco County, Florida and Coachella Valley, California. Using 1-week and 2-week filters, mosquito trap data are reproduced well by the model (P < 0.0001). Dry environments in southern California produce different mosquito population trends than moist locations in Florida. Florida and California mosquito populations are generally temperature-limited in winter. In California, locations are water-limited through much of the year. Using future climate projection data generated by the National Center for Atmospheric Research CCSM3 general circulation model, we applied temperature and precipitation offsets to the climate data at each location to evaluate mosquito population sensitivity to possible future climate conditions. We found that temperature and precipitation shifts act interdependently to cause remarkable changes in modeled mosquito population dynamics. Impacts include a summer population decline from drying in California due to loss of immature mosquito habitats, and in Florida a decrease in late-season mosquito populations due to drier late summer conditions.     

The developmental migration of Plasmodium in mosquitoes

Migration of the protozoan parasite Plasmodium through the mosquito is a complex and delicate process, the outcome of which determines the success of malaria transmission. The mosquito is not simply the vector of Plasmodium but, in terms of the life cycle, its definitive host: there, the parasite undergoes its sexual development, which results in colonization of the mosquito salivary glands. Two of the parasite's developmental stages in the mosquito, the ookinete and the sporozoite, are invasive and depend on gliding motility to access, penetrate and traverse their host cells. Recent advances in the field have included the identification of numerous Plasmodium molecules that are essential for parasite migration in the mosquito vector.     

Plasmodium differentiation in the mosquito

The essential passage of the malarial parasite through a mosquito vector results in major population bottlenecks in parasite numbers. The volume of the bloodmeal ingested by the female mosquito is 1-2 microliters. This may contain from 1 to 10(5) gametocytes. Of these, it is normal for just 12 to become macrogametes; 5-6 become ookinetes, and 2 develop into oocysts 2-7 days later. Of the 16,000 sporozoites produced from these two oocysts just 10-20 are inoculated by the malaria-infected female mosquito each time she probes when taking a subsequent bloodmeal. These significant population bottlenecks suggest that parasite differentiation is severely constrained by the environment in the mosquito, and therefore by the interactions between the parasite and the vector. This review will describe parasite differentiation in the mosquito and try to highlight the more important interactions between the parasite, the bloodmeal and the mosquito, attempting to identify those interactions which are essential to parasite differentiation, and those where the mosquito may be mounting effective strategies against this important pathogen. The potential exploitation of these interactions as possible mechanisms for intervention will be discussed.     

Vector infection determinants of Venezuelan equine encephalitis virus reside within the E2 envelope glycoprotein

Epizootic subtype IAB and IC Venezuelan equine encephalitis viruses (VEEV) readily infect the epizootic mosquito vector Aedes taeniorhynchus. The inability of enzootic subtype IE viruses to infect this mosquito species provides a model system for the identification of natural viral determinants of vector infectivity. To map mosquito infection determinants, reciprocal chimeric viruses generated from epizootic subtype IAB and enzootic IE VEEV were tested for mosquito infectivity. Chimeras containing the IAB epizootic structural gene region and, more specifically, the IAB PE2 envelope glycoprotein E2 precursor gene demonstrated an efficient infection phenotype. Introduction of the PE2 gene from an enzootic subtype ID virus into an epizootic IAB or IC genetic backbone resulted in lower infection rates than those of the epizootic parent. The finding that the E2 envelope glycoprotein, the site of epitopes that define the enzootic and epizootic subtypes, also encodes mosquito infection determinants suggests that selection for efficient infection of epizootic mosquito vectors may mediate VEE emergence.