The production of azurin and similar proteins

Summary It has been shown that, in the organisms tested, the production of azurin or similar blue, copper-protein complexes is confined to bacterial species of the three genera Bordetella, Alcaligenes and Pseudomonas. In the strain of Pseudomonas aeruginosa used, there appeared to be no difference in the amount of azurin occurring when the strain was grown aerobically and anaerobically. The amount of azurin produced by representative strains of Ps. aeruginosa, B. bronchiseptica and A. denitrificans varied with the copper content of the medium. Above a level of 5 g copper/ml of medium, the azurin content was constant for the three species tested; below a copper level of 0.5 g/ml there was an almost total absence of azurin although good growth occurred. Under similar growth conditions, the azurin content of the three bacterial species studied was not significantly different.The possible role of azurin is discussed.     

Cytokine and chemokine expression in a rat endometriosis is similar to that in human endometriosis

The pathogenesis of endometriosis, a gynecologic disorder associated with infertility, appears to involve immune responses. However, the details involved have not been clarified. In this study, we analyzed expression levels of interleukin (IL)-6, IL-10, monocyte chemoattractant protein-1, eosinophil chemotactic protein, macrophage inflammatory protein-1α, and regulated on activation normal T cell expressed and secreted (RANTES) and CC chemokine receptor 1 in endometriotic lesions in a rat model in which endometrium is autotransplanted onto peritoneal tissue and found that they were remarkably increased, while those of IL-2, IL-4, and interferon-γ were not. These results were obtained in a rat model induced by autologous, not allogeneic, transplantation of endometrial epithelium to the peritoneum. Expression of these factors is consistent with that of endometriosis in humans. Therefore, this model may be useful in the investigation of the pathogenesis and treatment of endometriosis.     

Power production among different sports with similar maximum strength

The purpose of this study was to compare 1 repetition maximum (1RM) strength, maximum power (MP), linear momentum (Mp), and the loads of 1RM responsible for maximum power (MP%) and for linear momentum (Mp%) generated in explosive concentric bench press (CBP) motion among athletes from different sports. A total of 56 athletes (13 sprinters, 16 basketball players, 16 handball players, 5 volleyball players, and 6 bodybuilders) performed CBP in the loads of 40, 50, 60, 70, and 80% of 1RM with 1-minute rest intervals. MP and Mp were assessed during CBP by customized apparatus with 3 separate displacement transducers. There were no significant differences in MP, Mp, MP%, and Mp% among the athletes. The only significant positive correlation was found between overall 1RM and Mp for athletes (r = 0.37). In conclusion, long-term sport-specific training adaptations do not play a major role on speed-strength parameters in athletes with similar strength from different sports backgrounds.     

Sex dependent behavioral response to frontal cortical suction lesions in the rat

Previous reports indicate that male rats given right (but not left) frontal cortical suction lesions develop hyperactivity accompanied by cortical norepinephrine depletions. In this study, female rats given such lesions to either hemisphere developed bilateral cortical norepinephrine depletions but no hyperactivity. Right lesion male rats developed both catecholamine depletions and hyperactivity. Thus, the lateralized hyperactivity response to these cortical lesions is sex-dependent and may involve sex differences in subcortical neural pathways.     

Experimental aflatoxin production in Manchego-type cheese

Manchego-type cheese, a typical Spanish cheese, was inoculated in various ways with an aflatoxigenic organism, Aspergillus parasiticus NRRL 2999, to study the production of aflatoxin. When the original milk was contaminated with a spore suspension, aflatoxin was not detected in paraffin-covered cheeses although it was present in the top layer of non-paraffin-covered cheeses after ripening at 15°C for 60 d. When the cheese surface was inoculated, no aflatoxins were detected in paraffin-covered cheeses after ripening for 60 d although they were found when the cheeses were ripened for 30 d. In non-paraffin-covered cheeses aflatoxins were detected only in the top layer and in the second 10 mm layer when cheeses were incubated after the normal ripening at 28°C for 30 d. When the centre of the cheese was inoculated, no aflatoxins were detected although Aspergillus grew slightly along the inoculation area. When cheese portions were inoculated, fungal growth was evident after incubation at 28° and 15°C for 6 d but there was no growth at 10°C after 50 d. At 28°C aflatoxins were detected at a concentration of 132 μg/g after 13 d, the highest level obtained. In cheese paste at 28° and 15°C, growth was intense, but the level of aflatoxins detected was lower than in cheese portions. At 10°C the growth was heavy, but aflatoxins were not detected.     

Experimental aflatoxin production in Manchego-type cheese

Manchego-type cheese, a typical Spanish cheese, was inoculated in various ways with an aflatoxigenic organism, Aspergillus parasiticus NRRL 2999, to study the production of aflatoxin. When the original milk was contaminated with a spore suspension, aflatoxin was not detected in paraffin-covered cheeses although it was present in the top layer of non-paraffin-covered cheeses after ripening at 15 degrees C for 60 d. When the cheese surface was inoculated, no aflatoxins were detected in paraffin-covered cheeses after ripening for 60 d although they were found when the cheeses were ripened for 30 d. In non-paraffin-covered cheeses aflatoxins were detected only in the top layer and in the second 10 mm layer when cheeses were incubated after the normal ripening at 28 degrees C for 30 d. When the centre of the cheese was inoculated, no aflatoxins were detected although Aspergillus grew slightly along the inoculation area. When cheese portions were inoculated, fungal growth was evident after incubation at 28 degrees and 15 degrees C for 6 d but there was no growth at 10 degrees C after 50 d. At 28 degrees C aflatoxins were detected at a concentration of 132 micrograms/g after 13 d, the highest level obtained. In cheese paste at 28 degrees and 15 degrees C, growth was intense, but the level of aflatoxins detected was lower than in cheese portions. At 10 degrees C the growth was heavy, but aflatoxins were not detected.