共查询到20条相似文献,搜索用时 15 毫秒
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V Chalupa 《Biologia Plantarum》1990,32(5):332-338
Shoot multiplication of hornbeam was stimulated onWPM, QL andDKW medium supplemented with a low concentration of BAP or BPA (0.1-0.2 mg I -1) andIBA (0.1 mg I -1). Low concentration of thidiazuron promoted axillary bud formation, higher concentration inhibited shoot elongation.
Microshoots were rooted onWPM supplemented with a low auxin concentration (IBA or NAA 0.2-0.5 mg I -1). High rooting percentages were obtained. Shoot proliferation
of ash was stimulated on MS andDKW medium supplemented withBAP orBPA (2.0-5.0 mg I -1) andIBA (0.1 mg I -1). Root formation was promoted onWPM containing a low auxin concentration. Rooted plantlets were transplanted into soil and after hardening off the micropropagated
trees were planted in the field. The planted trees grew normally without showing signs of abnormality. 相似文献
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扫帚菜、蕨菜营养成分分析 总被引:8,自引:0,他引:8
姚玉霞 《氨基酸和生物资源》2003,25(1):1-2
对扫帚菜、蕨菜两种山野菜的粗蛋白、粗脂肪、膳食纤维、胡萝卜素、氨基酸、维生素及微量元素等营养成分进行了分析.结果表明,两种山野菜营养丰富,各种营养成分较为齐全,为开发食用山野菜等绿色食品提供科学依据和参考. 相似文献
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M Ro?ek 《Folia biologica》1989,37(3-4):151-154
Bembidion quadrimaculatum possesses 24 chromosomes: 2n male = 22 + XY, 2n female = 22 + XX; their structure is meta- and submetacentric and differences in length between them are slight. Achiasmatic meiosis has been identified in spermatogenesis. The diploid chromosome number in Clivina fossor is 44; 2n male = 42 + XY, 2n female = 42 + XX. The chromosome structure is meta-, submeta-, and subtelocentric and X is the longest element in the set. 1 to 2 chiasmata per bivalent occur in meiosis. 相似文献
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Cells of Glycine max originating in a suspension culture and cells of Brassica napus prepared from hypocotyls were synchronized. Synchronization was achieved by preparing protoplasts in the usual way and subsequently letting the protoplasts regenerate into cells by removing the cell-wall-digesting enzymes. More than 70% of the cells had divided synchronously at the end of the first cycle as determined by the mitotic index. The high frequency of mitosis critically depended on the osmolality of the medium. The duration of the S-phase was estimated by measuring the activity of thymidylate kinase as well as incorporation of [3H]deoxythymidine into acid-insoluble material. The data indicate that synchronization is induced by resetting the cell cycle.Abbreviations dTMP
deoxythymidine 5-monophosphate
- TCA
trichloroacetic acid 相似文献
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Freezing of adult flounder Platichthys flesus and turbot Psetta maxima for 24 h resulted in mean decrease of total length ( L T ) of 2·1 and 1·3% and a reduced body mass ( M ) of 3·1 and 1·3%, respectively. Increasing the freezing time to 25 days increased the shrinkage in turbot but not in flounder. The L T and M T conversion equations for the different species and time spent frozen are given. The differences between species could be explained by the higher volume to surface area relationship in turbot compared with flounder. 相似文献
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W. Y. Cheung G. Champagne N. Hubert L. Tulsieram D. Charne J. Patel B. S. Landry 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(1-2):73-82
Self-incompatibility (SI) in Brassica is a sporophytic system, genetically determined by alleles at the S-locus, which prevents self-fertilization and encourages outbreeding. This system occurs naturally in diploid Brassica species but is introduced into amphidiploid Brassica species by interspecific breeding, so that in both cases there is a potential for yield increase due to heterosis and the
combination of desirable characteristics from both parental lines. Using a polymerase chain reaction (PCR) based analysis
specific for the alleles of the SLG (S-locus glycoprotein gene) located on the S-locus, we genetically mapped the S-locus of B. oleracea for SI using a F2 population from a cross between a rapid-cycling B. oleracea line (CrGC-85) and a cabbage line (86-16-5). The linkage map contained both RFLP (restriction fragment length polymorphism)
and RAPD (random amplified polymorphic DNA) markers. Similarly, the S-loci were mapped in B. napus using two different crosses (91-SN-5263×87-DHS-002; 90-DHW-1855-4×87-DHS-002) where the common male parent was self-compatible,
while the S-alleles introgressed in the two different SI female parents had not been characterized. The linkage group with the S-locus in B. oleracea showed remarkable homology to the corresponding linkage group in B. napus except that in the latter there was an additional locus present, which might have been introgressed from B. rapa. The S-allele in the rapid-cycling Brassica was identified as the S29 allele, the S-allele of the cabbage was the S
5 allele. These same alleles were present in our two B. napus SI lines, but there was evidence that it might not be the active or major SI allele that caused self-incompatibility in these
two B. napus crosses.
Received: 7 June 1996/Accepted: 6 September 1996 相似文献
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Summary A combination of a removal and fertilization experiment in the field and a replacement experiment in containers in a cold frame was carried out to investigate the role of nutrient supply in the interactions between Calluna vulgaris and Molinia caerulea. It is concluded that the growth of Calluna as well as that of Molinia increased with increasing nutrient availability. However, the increase in biomass of Molinia was much larger than that of Calluna. It is also concluded that increased nutrient availability favours the competitive vigour of Molinia in interaction with Calluna. 相似文献
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Fluorescent pseudomonads were isolated from the rhizosphere of rice and sugarcane and examined for their biodiversity. All fifty strains of the fluorescent pseudomonads produced indole acetic acid. Among these pseudomonads, halves of sugarcane rhizosphere isolates and one isolate from the rice rhizosphere exhibited phosphate solubilization activity. On the contrary, majority of the rice rhizosphere pseudomonads, and one isolate from sugarcane rhizosphere exhibited antifungal activity. These fluorescent pseudomonads were further classified based on their growth and biochemical characteristics. Those isolates that had same biochemical characteristics were distinguished by random amplification of polymorphic DNA (RAPD). These biochemical and molecular biological methods clearly differentiated fluorescent Pseudomonads of rice and sugarcane rhizosphere. 相似文献
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Different cultivars of cow pea and garden pea seeds were surveyed for susceptibility or resistance towards the toxigenic and
aflatoxin-producing mould (Aspergillus flavus IMI 102135). The results show that aflatoxin production varied among the different cultivars of both cow pea and garden pea.
Morphological and histological characters of the different cultivars tested did not show any relation between colour, shape
and size of seeds and the amount of aflatoxin produced.
The chemical analysis of the different constituents obtained from both seed coats and seed kernels with susceptible, partially
resistant and resistant cow pea and garden pea cultivars revealed that the resistant cultivars of cow pea (namely: Balady
cultivar) and garden pea (namely: Melting Sugar cultivar) contained lower levels of sodium and higher levels of phosphate
and potassium. 相似文献
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A Stratil V Tomásek J R Clamp J Williams 《Comparative biochemistry and physiology. B, Comparative biochemistry》1985,80(4):909-911
Basic composition and properties of isolated transferrins of Silurus glanis and Esox lucius have been compared. In transferrin of S. glanis carbohydrate is absent, but it is present in transferrin of E. lucius (2.5%). The N-terminal amino acid is alanine in both species. Mol. wts are 68,400 (S. glanis) and 86,800 (E. lucius). Transferrins of the two species are heterogeneous, but genetic polymorphism was not observed. 相似文献
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We investigated the change of root net proton excretion of seedlings of Triticum aestivum L. and Zea mays L. with daily variation of illumination using a multi-channel pH-stat system. We found an increase of net proton excretion during darkness and a drop after the beginning of illumination. Inhibition of carotenoid biosynthesis by norflurazone and photooxidation of chlorophylls did not change the periodicity or its induction. The induction of diurnal periodicity was possible with blue, green and red light. After induction the oscillation of net proton excretion continued for at least two cycles under constant light. We conclude that net H+ excretion of wheat and maize roots may be regulated by an endogenous clock or by a signal from the leaves. The nature of such a hypothetical signal remains unknown. 相似文献
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H. Peterka H. Budahn O. Schrader 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(3-4):383-389
Interspecific hybrids between Allium cepa and A. ampeloprasum have been generated as a first step for the introduction of S-cytoplasm from onion into leek. Pre-zygotic barriers of crossability
were observed after the arrival of pollen tubes at the end of the style when entering the cavity. Nevertheless, micropyle
penetration of pollen tubes and the formation of hybrid embryos were also observed. After accomplishing in vitro culture of
ovaries and ovules successively, triploid hybrid plants with 24 chromosomes were obtained. Their hybrid nature was confirmed
by RAPD analysis, genomic in situ hybridization, and morphological analysis. Southern hybridization with a cytoplasmic probe
indicated the transfer of unaltered S-cytoplasm into the hybrid plants.
Received: 9 May 1996 / Accepted: 23 August 1996 相似文献
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Dr. Paul Pevet 《Cell and tissue research》1975,159(3):303-309
Special cells in the hedgehog and mole pineal gland which are characterized by an enormous single vacuole were studied. The presence of secretory granules (in the hedgehog) and of paracrystalline structures (in the mole) in the cytoplasm of these cells indicates that they are pinealocytes. 相似文献
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The potential of water lettuce (Pistia stratiotes L.) and duckweed (Lemna minor L.) to remove chlorpyrifos in water was investigated under laboratory greenhouse conditions. At initial chlorpyrifos concentrations of 0.0, 0.1 and 0.5 mg/L, the relative growth rates (RGR) of L. minor and P. stratiotes were not significantly different. In contrast, in the presence of 1 mg/L chlorpyrifos the RGR was significantly inhibited, giving an observed fresh weight based RGR(FW) for P. stratiotes and L. minor from day 0 to 7 of -0.036 and -0.023 mg/g/day, respectively. The maximum removal of chlorpyrifos by P. stratiotes and L. minor, when chlorpyrifos was at an initial culture concentration of 0.5 mg/L, was 82% and 87%, respectively, with disappearance rate constants under these conditions of 2.94, 10.21 and 12.14 microg h(-1) for the control (no plants), and with P. stratiotes and L. minor, respectively, giving actual corrected plant removal rate constants of 7.27 and 9.20 microg h(-1) for P. stratiotes and L. minor, respectively. The bioconcentration factor (BCF) of L. minor was significantly greater than that for P. stratiotes and therefore, at least under these greenhouse-based conditions, L. minor was more efficient than P. stratiotes for the accelerated removal of chlorpyrifos from water. 相似文献
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This paper continues to explore niche differentiation in Gammarus pulex and Asellus aquaticus by analysis of their food preferences. Individuals from both species discriminated between leaf discs colonized by different fungal species and exhibited strong preferences for Anguillospora longissima and Heliscus lugdunensis. Fungal preferences were not correlated with the relative abundance of fungi in the field and there was considerable intra-population variability in food preferences — both between individuals and for the same individual through time. Niche overlap between animals from all four study populations was high and there was no evidence of differences in the potential trophic niches of animals from sympatric and allopatric populations. 相似文献
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