共查询到20条相似文献,搜索用时 15 毫秒
1.
A. D'Hont P. S. Rao P. Feldmann L. Grivet N. Islam-Faridi P. Taylor J. C. Glaszmann 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1995,91(2):320-326
Molecular markers were used to characterise sugarcane intergeneric hybrids between S. officinarum and E. arundinaceus. Very simple diagnostic tools for hybrid identification among the progeny were derived from isozyme electrophoresis and a sequence-tagged PCR. Two enzyme systems (GOT and MDH B) and PCR amplification revealing spacer-size variation in the 5s-rDNA cluster were found most convenient. Specific characterisation of the two genomic components was possible using RFLP and in situ hybridisation. The strong molecular differentiation between S. officinarum and E. arundinaceus allows the identification of numerous Erianthus-specific RFLP bands in the hybrids. Genomic DNA in situ hybridisation allows for the differentiation of the chromosomes contributed by S. officinarum and E. arundinaceus in chromosome preparations of the hybrids. In situ hybridisation with the 18s-5.8s-25s rDNA probe highlights the basic chromosome numbers in the two parental species. The potential of these techniques to monitor the Erianthus genome during the introgression process is discussed. 相似文献
2.
M. S. Kang B. Glaz J. D. Miller 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1987,74(3):310-316
Summary The stability-variance statistic, s
i
2
, measures the contribution of the ith genotype to genotype x environment interaction. In addition to the knowledge of cultivar stability for an agronomic trait, information on whether stability of one trait can be used to predict stability of another should be useful to breeders. Three separate groups of data, respectively involving CP 79 series, CP 80 series, and CP 81 series experimental clones of sugarcane (Saccharum spp.) were used in this study. Rank-correlation coefficients (rs) between ranks of genotypes for s
i
2
's for paired traits indicated in both plant-cane and ratoon crops that stability of tons per hectare of sugar can be predicted from the stability of tons per hectare of cane (THC) and also, to a lesser extent, from the stability of stalk number. The stability of THC also can be reasonably well predicted from the stability of stalk number. Brix stability may give some indication of the stabilities for percentage sucrose and sugar concentration (SC). The s
i
2
's for percentage sucrose and SC were almost identical in the CP 79 and CP 81 series (rs varied from 0.93, P<0.01, in plant-cane crop for CP 79 series to 0.98, P<0.01, in plant-cane crop for CP 81 series). Whether correlations were based on s
i
2
's estimated across locations within crops or across crops, the magnitude of rs was about the same. Means of various traits were not correlated with their respective s
i
2
's (for CP 81 series), indicating that identification and selection of high-yielding sugarcane genotypes with a relatively high degree of stability of performance across test environments should be possible.Cooperative investigation of the Univ. of Florida, Everglades Research and Education Center, Belle Glade, FL, USA; Louisiana Agricultural Experiment Station, LSU Agricultural Center, Baton Rouge, LA, USA; and Sugarcane Field Station, Canal Point, FL, USA. The field work reported in this study was done when the senior author was affiliated with the University of Florida. Florida Agric. Exp. Stns. Journal Series No. 5933 相似文献
3.
Summary With the aim to determine a possible relationship between somatic embryogenesis and some metabolic contents in embryogenic
and non-embryogenic calluses of sugarcane (Saccharum sp. var CP-5243), the present study was carried out. Embryogenic callus has more soluble proteins, free proline, proteolytic
activity, soluble sugars, and invertase, and lower putreseine/(spermidine + spermine) than non-embryogenic tissue. Non-embryogenic
callus has a higher peroxidase and gallic acid level, lower dry matter/fresh matter ratio, and more gross fat compared with
embryogenic callus. 相似文献
4.
Sucrose partitioning between vascular bundles and storage parenchyma in the sugarcane stem: a potential role for the ShSUT1 sucrose transporter 总被引:6,自引:0,他引:6
A transporter with homology to the SUT/SUC family of plant sucrose transporters was isolated from a sugarcane (Saccharum hybrid) stem cDNA library. The gene, designated ShSUT1, encodes a protein of 517 amino acids, including 12 predicted membrane-spanning domains and a large central cytoplasmic loop. ShSUT1 was demonstrated to be a functional sucrose transporter by expression in yeast. The estimated Km for sucrose of the ShSUT1 transporter was 2 mM at pH 5.5. ShSUT1 was expressed predominantly in mature leaves of sugarcane that were exporting sucrose and in stem internodes that were actively accumulating sucrose. Immunolocalization with a ShSUT1-specific antiserum identified the protein in cells at the periphery of the vascular bundles in the stem. These cells became lignified and suberized as stem development proceeded, forming a barrier to apoplasmic solute movement. However, the movement of the tracer dye, carboxyfluorescein from phloem to storage parenchyma cells suggested that symplasmic connections are present. ShSUT1 may have a role in partitioning of sucrose between the vascular tissue and sites of storage in the parenchyma cells of sugarcane stem internodes. 相似文献
5.
High output genetic mapping of polyploids using PCR-generated markers 总被引:16,自引:0,他引:16
Bruno W. S. Sobral Rhonda J. Honeycutt 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1993,86(1):105-112
Summary The polymerase chain reaction (PCR) with arbitrarily selected primers has been established as an efficient method to generate fingerprints that are useful in genetic mapping and genomic fingerprinting. To further increase the productivity of mapping and fingerprinting efforts, we have altered existing protocols to include the use of the Stoffel fragment, which is derived from genetically engineered Taq polymerase. We also optimized the thermal profile of the reaction to increase the number of useful primers. In mapping of the genome of Saccharum spontaneum SES 208, a polyploid wild relative of sugarcane, these modifications allowed for an increase of 30% in the number of loci screened per primer, and an 80% increase in the number of polymorphisms per primer. Furthermore, the enzyme cost per reaction was decreased approximately 1.6-fold. Finally, there was an increase from about 70% to about 97% in the number of primers that were useful (i.e., gave a reproducible fingerprint) using our protocol. We have placed some of these markers into linkage groups. 相似文献
6.
Maira Oropeza Palmira Guevara Eva de García José Luis Ramírez 《Plant Molecular Biology Reporter》1995,13(2):182-191
Somaclonal variants resistant to sugarcane mosaic virus (SCMV) were obtained from susceptible sugarcane cv PR62258 through
somatic embryogenesis by increasing the number of subcultures of the embryogenic callus tissue in MS medium with 3 mg/L 2,4-dichlorophenoxyacetic
acid. Transfers were made at 30-day intervals for 1, 2 or 3 subcultures. Two somaclones, namely AT626 and BT627, were selected
by their resistance to SCMV. These subclones have maintained the resistance trait over seven years of testing in the field.
In this report we identified the somaclonal SCMV resistant variants from the maternal line and the nonresistant somaclones,
using the RAPD technique. 相似文献
7.
S. K. Thukral D. R. Satija V. P. Gupta 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1986,71(4):648-651
Summary The study of phenolic content and activities of peroxidase and polyphenoloxidase in relation to the degree of downy mildew infection of 12 pearl millet cultivars revealed that these were linearly related to the degree of resistance at both the 30 and 50 day growth stages. Useful electrophoretic differences in peroxidase and polyphenoloxidase were also observed with respect to the expression of resistance. 相似文献
8.
Development of genetic markers in celery based on restriction fragment length polymorphisms 总被引:2,自引:0,他引:2
G. M. Huestis J. M. McGrath C. F. Quiros 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1993,85(6-7):889-896
Summary Linkage relationships are reported for 34 markers in celery (Apium graveolens L. var dulce) including 21 RFLP, 11 isozyme, and 2 morphological traits. The mapping was carried out in a cross between celery and an annual accession from Thailand, A143, and based on F2 segregation of 136 plants. A total of 318 centiMorgans (cM) are covered by the markers distributed in 8 linkage groups. Probes for the identification of RFLPs were isolated from a celery cDNA library and were also obtained from heterologous sources. EcoRV, EcoRI, and HindIII were the most useful restriction enzymes in uncovering polymorphism. In our cross, 18% of the cDNA probes were found to be polymorphic for at least one of the enzymes used. Six of the markers showed significant deviations from expected F2 ratios. 相似文献
9.
High-efficiency,microprojectile-mediated cotransformation of sugarcane,using visible or selectable markers 总被引:3,自引:0,他引:3
Robert Bower Adrian R. Elliott Bernard A. M. Potier Robert G. Birch 《Molecular breeding : new strategies in plant improvement》1996,2(3):239-249
Transient expression of the maize anthocyanin regulatory elements,R andC1, was used to optimise parameters for microprojectile-mediated delivery of DNA into sugarcane embryogenic callus. Osmotic treatment of target tissues and particle acceleration in a high-pressure helium pulse increased the frequency of transient expression to 5–8×103 cells per bombardment, with minimal tissue damage. An average of 0.34% of transiently expressing cells developed into stably transformed, anthocyanin-pigmented proembryoids which subsequently regenerated into plantlets. However, constitutive expression ofR andC1 proved deleterious, and no anthocyanin-pigmented plant survived beyond 3 cm in height. We also compared selective subculture of callus portions showing luciferase activity with antibiotic selection on medium containing G418 or phosphinothricin, upon bombardment of callus with constructs driving strong expression ofluc, aphA orbar genes. Selective subculture based on luciferase activity enabled recovery of 1.4±0.5 independent transgenic plants per bombardment, compared to 19.8±3.7 independent transgenic plants per bombardment from an optimised G418 selection regimen, and no transformed plants from phosphinothricin selection. Whenluc andaphA on separate plasmids were coprecipitated onto microprojectiles before bombardment, 67–79% of callus lines selected for G418 resistance also showed luciferase activity detectable under a low-light camera. Southern analysis confirmed a very high cotransformation frequency, with variable copy numbers of introduced genes. The high efficiencies of gene transfer, selection and cotransformation in the optimised system, coupled with the simple initiation and regeneration of embryogenic callus, provide an effective tool for practical genetic transformation of sugarcane. 相似文献
10.
P. M. Gaur A. E. Slinkard 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1990,80(5):648-656
Summary Allozyme polymorphisms of nine enzymes — aspartate aminotransferase (AAT), diaphorase (DIA), esterase (EST), formate dehydrogenase (FDH), -galactosidase (GAL), -glucosidase (GLU), malate dehydrogenase (MDH), malic enzyme (ME), and peroxidase (PRX) — were described in chick-pea (Cicer L.). Thirteen isozyme loci, Aat-c, Dia-4, Est-2, Est-4, Est-10, Fdh, Gal-2, Gal-3, Gal-4, Glu-3, Mdh-2, Me-2, and Prx-2, were genetically defined. Alleles of each of these isozyme loci expressed codominantly in heterozygotes and exhibited a codominant, single-locus segregation ratio in F2. The loci Est-2, Mdh-2, and Me-1 were expressed only in flower. Linkage relations were determined for these 13 and several previously defined isozyme loci. The following new genetic linkages were identified: Pgm-p (locus for plastid phosphoglucomutase) — Est-10; Ald-p1 (one of the duplicate loci for plastid aldolase) — Glu-3 — Gal-2 — Est-2,3; Gal-3 — Aco-m (locus for mitochondrial aconitase) — Prx-2,3; Gpi-c (locus for cytosolic glucosephosphate isomerase) — Fdh; and Est-4 — Me-1. This study provides further confirmation on the existence of several conserved linkage groups among Cicer, Pisum, and Lens. 相似文献
11.
J. E. Irvine 《Plant Cell, Tissue and Organ Culture》1984,3(3):201-209
Leaf tissue from five sugarcane clones with distinctive markers was cultured on a medium favoring callus growth. Transferred to a differentiation medium, calli produced over 5000 plants. Plants differentiated from two clones with stem markers exhibited a high rate of remission of the marker, but the marker reappeared in the vegetative progeny of these plants, and remission was, therefore, transient. Plants differentiated from callus from two clones with leaf markers showed a low rate of remission (2 or 3 per thousand) of the marker and the vegetative progeny was stable. A clone with variegated leaves produced plants with the majority having green leaves, some were albino, and some variegated, suggesting that plant differentiation may start with more than one cell. Permanent phenotypic change may result from tissue culture, but the results suggest that such changes are not frequent and may be confounded by temporary alterations or by chimeras formed in the process of differentiation. 相似文献
12.
E. Smed J. P. C. Van Geyt M. Oleo 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1989,78(1):97-104
Summary Five isozyme systems were genetically investigated. The different separation techniques, the developmental expression and the use as marker system in sugar beet genetics and breeding is discussed. Isocitrate dehydrogenase was controlled by two genes. The gene products form inter- as well as intralocus dimers, even with the gene products of the Icd gene in B. procumbens and B. patellaris. Adenylate kinase was controlled by one gene. Three different allelic forms were detected, which were active as monomeric proteins. Glucose phosphate isomerase showed two zones of activity. One zone was polymorphic. Three allelic variants, active as dimers, were found. Phosphoglucomutase also showed two major zones of activity. One zone was polymorphic and coded for monomeric enzymes. Two allelic forms were found in the accessions studied. The cathodal peroxidase system was controlled by two independent genes, of which only one was polymorphic. The gene products are active as monomers. Linkage was found between red hypocotyl color (R) and Icd
2. Pgm
1, Gpi
2, Ak
1 and the Icd
2-R linkage group segregated independently. 相似文献
13.
Z. Satovic A. M. Torres J. I. Cubero 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1996,93(7):1130-1138
Thirteen F2 families of faba bean (Vicia faba L.), descended from plants trisomic for chromosomes 3, 4, 5 and 6, have been analyzed for morphological, isozyme and RAPD markers. This allowed the establishment of linkage relationships among these markers as well as the assignment of some markers and/or linkage groups to their respective chromosomes. The linkage analysis of partially overlapping sets of informative genetic markers for the data pooled from 13 F2 families has revealed 48 linkage groups, six of which have been precisely assigned to specific chromosomes. A statistical procedure to analyze the data of joint segregation analysis in families derived from trisomic plants has been developed. 相似文献
14.
J. C. Lorenzo E. Ojeda A. Espinosa Carlos Borroto 《In vitro cellular & developmental biology. Plant》2001,37(6):803-806
Summary The temporary immersion bioreactor has been found to be an important tool for sugarcane micropropagation, allowing higher
shoot formation rates and cost reduction. This research was conducted to demonstrate the agricultural value of temporary immersion
bioreactor-derived sugarcane plants. The experiment was carried out for about 2 yr to study the field performance of these
plants. Two control treatments were also evaluated representing the conventional forms of micro- and macropropagation. Growth
of sugarcane stools, first ratoon and the use of micropropagated plants for macropropagation were recorded. Some botanical
and chemical characteristics were evaluated. Differences among propagation systems were only found in the first 6 mo. of field
growth, regarding the stem length and diameter. Such differences disappeared with the course of the experiment. 相似文献
15.
K. Chengalrayan M. Gallo-Meagher 《In vitro cellular & developmental biology. Plant》2001,37(4):434-439
Summary Sugarcane (Saccharum spp. hybrid cv. CP 84-1198) embryogenic calluses were induced from young leaves cultured on modified Murashige and Skoog
basal medium supplemented with 13.6 μM 2,4-dichlorophenoxyacetic acid. Five concentrations, 0.5, 1.0, 2.5, 5.0, and 10.0 μM, of five different growth regulators, 6-benzylaminopurine, kinetin, 6-γ,γ-(dimethylallylamino)purine, zeatin, and thidiazuron,
were tested with or without 22.5 μM α-naphthaleneacetic acid to compare their ability to induce regeneration from embryogenic callus. After 4 wk on medium, the
percentage of shoot meristem induction was evaluated, and after 10 wk the total number of shoots produced, as well as the
percentage of shoots greater than 1 cm in length, was obtained. Although it had the lowest percentage of elongated shoots,
medium containing thidiazuron alone performed better than all other growth regulators tested, with the highest percentage
of shoot induction and the largest number of shoots, particularly at a concentration of 2.5 μM. 相似文献
16.
Isozymes and random amplified polymorphic DNA (RAPD) markers were used for precocious identification of non-maternal plants in progenies of the facultative apomict Poa pratensis. Four progenies obtained from controlled crosses that showed different degrees of apomixis on isozyme analysis of phospho-gluco-isomerases, esterases and peroxidases were chosen for RAPD analysis to generate genomic fingerprints using species-specific primers. At an advanced vegetative stage, a morphological analysis was also performed and characteristics related to growth habit and leaf morphology were observed and recorded. On the basis of the isozyme and RAPD electrophoretic pattern and the morphological appearance, each plant was classified as maternal or aberrant. All three classes of genetic markers employed were able to identify plants that exhibited aberrant traits in the four progenies. Overall, the results of RAPD analysis supported those of isozyme and morphology studies. However, in each progeny, some plants which both isozyme and morphological analyses distinguished as of maternal origin were aberrant according to RAPD analysis. Therefore, the RAPD method proved the most precise screening technique. The greater cost of the molecular approach was offset by its higher accuracy. The use of either three isozyme systems or six primers for PCR amplification seems to be sufficient for reliable estimation of the degree of apomixis. Histological analyses were carried out and the aposporic development of the plant material studied. 相似文献
17.
K. K. Wu D. J. Heinz D. M. Hogarth 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1988,75(5):754-760
Summary This study was conducted to estimate the degree of association of smut (Ustilago scitaminea Syd.) resistance in sugarcane (Saccharum spp.) between races A and B in Hawaii and to estimate the heritability of resistance to both races. The estimated degree of association was 0.18. Although statistically significant, the degree of association of resistance between races A and B was near zero and therefore of no practical importance. Selection for resistance to one race would have little effect on the frequency of resistance to the other race in the following sexual generation. Heritabilities in the broad sense estimated from the parent population on a plot mean basis were 0.96 and 0.91 for races A and B, respectively. Selection for smut resistance should be very effective between populations of asexual generations. Heritabilities in the narrow sense estimated from parent-progeny regression analysis on a family mean basis were 0.51 and 0.47 for races A and B, respectively. Selecting and breeding for resistance should result in a fairly rapid increase in the frequency of resistance in the progeny population.Contribution from Department of Genetics and Pathology, Hawaiian Sugar Planters' Assn. Exp. Stn., P.O. Box 1057, Aiea, HI 96701. Published with the approval of the Director as paper no. 652 in the Journal Series of the Exp. Stn., Hawaiian Sugar Planters' Assn. Research funded in part by USDA/ARS Cooperative Agreement No. 58-9AHZ-0-492 相似文献
18.
Summary A cryopreservation process using encapsulation/dehydration was set up for apices sampled on in vitro plantlets of sugarcane. After dissection, apices were cultured for one day on standard medium and then encapsulated in medium with 3% alginate. Optimal conditions comprised preculture for 2 days in liquid medium with 250 g.l–1 sucrose, desiccation for 6 hours under the laminar flow or for 10–11 hours with silicagel followed by rapid freezing and slow thawing. Survival after freezing in liquid nitrogen ranged between 38 and 91% for the 5 varieties experimented. Cryopreservation did not modify the electrophoretic profiles for aminoleucine peptidases and amylases with plants of the variety Co 6415.Abbreviations BAP
6-benzylaminopurine
- KIN
Kinetin
- EDTA
ethylenediamine tetracetic acid
- AMP
aminoleucine peptidases
- AMY
amylases
- RFLP
restriction fragment length polymorphism 相似文献
19.
Nadina Nieves Yumaris Zambrano Raúl Tapia Mariela Cid Danilo Pina Ramiro Castillo 《Plant Cell, Tissue and Organ Culture》2003,75(3):279-282
This study shows the behaviour of sugarcane plants cv. CP-5243 derived from artificial seed compared with traditional and isolated bud methods. Artificial seed-acclimatised plants were planted in field conditions simultaneously with two-control treatments previously germinated: macropropagated plants derived from stems of three buds and axillary buds isolated from field-grown plants. Plants from artificial seed were taller and had a smaller diameter at 8 months, but these differences disappeared at 12 months. With respect to sugar analysis and yield, no differences in all parameters evaluated were found between artificial seed-derived plants and plants derived from the two other methods. 相似文献
20.
Li JZ Sjakste TG Röder MS Ganal MW 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2003,107(6):1021-1027
To enhance the marker density of existing genetic maps of barley (Hordeum vulgare L.), a new set of microsatellite markers containing dinucleotide motifs was developed from genomic clones. Out of 254 primer pairs tested, a total of 167 primer pairs were classifed as functional in a panel of six barley cultivars and three H. spontaneum accessions, and of those, 127 primer pairs resulting in 133 loci were either mapped or located onto chromosomes. The polymorphism information content (PIC) ranged from 0.05 to 0.94 with an average of 0.60. The number of alleles per locus varied from 1 to 9. On average, 3.9 alleles per primer pair were observed. The RFLP frameworks of two previously published linkage maps were used to locate a total of 115 new microsatellite loci on at least one mapping population. The chromosomal assignment of 48 mapped loci was corroborated on a set of wheat-barley chromosome addition lines; 18 additional loci which were not polymorphic in the mapping populations were assigned to chromosomes by this method. The microsatellites were located on all seven linkage groups with four significant clusters in the centromeric regions of 2H, 3H, 6H and 7H. These newly developed microsatellites improve the density of existing barley microsatellite maps and can be used in genetic studies and breeding research.Communicated by G. Wenzel 相似文献