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1.
Bud break and multiple shoots were induced in apical and axillary meristems derived from 10-d old seedlings of Madhuca longifolia var. latifolia on Murashige and Skoog (MS) medium supplemented with 1.0 mg/l N6-benzyladenine (BA) singly or in combinatiobn with 1-naphthalene acetic acid (NAA), indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA). Excised shoots were rooted on half-strength MS with IBA (1.0 mg/l) after 18d of culture. Regenerated plantlets were acclimatized and successfully transferred to soil.Abbreviations BA
N6 benzyladenine
- KN
kinetin
- ADS
adenine sulphate
- IBA
indole-3-butyric acid
- IAA
indole3-acetic acid
- NAA
1-naphthaleneacetic acid
- MS
Murashige and Skoog (1962) medium 相似文献
2.
High-frequency bud break and multiple shoots were induced in apical shoot buds and nodal explants ofMorus cathayana, M. lhou andM. serrata on Murashige and Skoog (MS) medium containing 0.5–1.0 mg/l 6-benzylaminopurine (BAP). Addition of gibberellic acid (0.4 mg/l) along with BAP induced faster bud break both in apical shoot buds and nodal explants and also enhanced the frequency of bud break in all three species. Shoot culture initiation was greatly influenced by explant type, explant age and explanting season. The shoots were successfully rooted on half-strength MS medium containing a combination of indole-3-acetic acid, indole-3-butyric acid and indole-3-propionic acid, each at 1.0 mg/l. The plantlets were successfully acclimated and eventually established in soil.Abbreviations
BAP
6-Benzylaminopurine
-
GA
3
Gibberellic acid
-
IAA
Indole-3-acetic acid
-
IBA
Indole-3-butyric acid
-
IPA
Indole-3-propionic acid
-
Kn
Kinetin
-
MS
Murashige and Skoog (1962) medium
-
NAA
1-Naphthalene acetic acid 相似文献
3.
D. A. Godbole M. N. Kunachgi U. A. Potdar K. V. Krishnamurthy A. F. Mascarenhas 《Plant cell reports》1984,3(2):75-78
Plantlets regenerated from shoot apices, cotyledons and callus cultures in Moth bean, Vigna aconitifolia (JACQ) Marechal, a drought resistant legume and pulse crop, were rooted and transferred to soil. Explants for these studies were derived from seedlings pre-conditioned by germination of seeds on B5BA and WMB (control).Abbreviations MS
Murashige and Skoog (1962)
- B5
B5 basal medium (Gamborg et al 1968)
- B5BA
B5 basal medium containing BA (2.25 mg/l)
- WMB
Modified White's medium (Mascarenhas et al 1976)
- BA
6-benzyladenine
- IAA
indole-3-acetic acid
- NAA
1-napthaleneaceticacid
- 2,4-D
2,4-dichlorophenoxyacetic acid
- IBA
indolebutyric acid
- 2iP
N(–2 isopentyl) adenine
- CM
coconut milk
NCL Communication No. 3375 相似文献
4.
Adventitious shoots were formed through callus on leaf explants of Eucalyptus camaldulensis Dehnh. (River red gum) taken from shoot cultures of mature trees. Callus formed in dark on a medium containing 1 g/l casein hydrolysate, 3 mg/l 1-naphthaleneacetic acid, 0.1 mg/l 6-benzyladenine and 50 g/l sucrose. Shoot initiation occurred in 4 weeks on calli shifted to light on a regeneration medium containing 10% coconut milk, 0.5 mg/l 6-benzyladenine and 20 g/l sucrose. Rooting occured in dark on a liquid medium containing 4 mg/l 1-naphthaleneacetic acid. Zygotic embryos of Eucalyptus citriodora Hook f. (Lemon scented gum) cultured in dark on a medium containing 3 mg/l 1-naphthaleneacetic acid and 50 g/l sucrose formed somatic embryoids which grew to normal plantlets on the same regeneration medium used for organogenesis.Abbreviations BAP
6-benzyladenine
- CH
Casein hydrolysate
- CM
Coconut Milk
- NAA
1-naphthaleneacetic acid
NCL Communication no. 4162 相似文献
5.
Songul Gurel Mehmet Cengiz Baloglu Ekrem Gurel Huseyin Avni Oktem Meral Yucel 《Plant Cell, Tissue and Organ Culture》2011,106(2):261-268
The effects of a two-stage pretreatment of seedlings on the subsequent shoot regeneration capacity were investigated. Pretreated
seedlings were obtained by germinating seeds on three different germination media and then further culturing on six different growth media. Lamina and petiole explants of two sugar beet (Beta
vulgaris L.) breeding lines were then excised from the pretreated seedlings and cultured on five different shoot regeneration media. In both breeding lines, petiole explants produced significantly more shoots than lamina explants with higher frequencies
of organogenic capacities; petiole explants of the lines M1195 and ELK345 produced a mean of 2.1 and 2.7 shoots per explant
while their lamina explants produced 1.5 and 2.2 shoots per explant, respectively. A genotypic variation was evident as the
line ELK345 was more productive for shoot development from both types of explants. In overall comparisons of different germination, growth and regeneration media, germination medium was most effective when supplemented with 0.5 mg/l 6-benzyladenine (BA) while both growth and regeneration
media were most productive when contained a combination of 0.25 mg/l BA and 0.10 mg/l indole-3-butyric acid (IBA). Of all
the treatments tested, the highest mean number of shoots per explant (8.3 shoots) and frequency of organogenic explants (75.6%)
were obtained on regeneration medium supplemented with 0.25 mg/l BA and 0.10 mg/l IBA when petiole explants of the line ELK345
were excised from the seedlings that had been germinated on medium containing 0.5 mg/l BA followed by further growth on medium
containing 0.25 mg/l BA and 0.10 mg/l IBA. 相似文献
6.
The study was carried out to establish in vitro culture conditions for plant regeneration of tef, Eragrostis tef (Zucc.) Trotter. Mature seeds of two Ethiopian varieties, DZ-01-354 and DZ-01-196, were used to initiate callus cultures
on Murashige and Skoog (MS) medium with different auxins. Four- and 8-week-old calli induced on a medium with 2.0 mg/l 2,4-dichlorophenoxyacetic
acid (2,4-D) were subcultured onto various media to induce somatic embryogenesis. Compact, nodulated, embryogenic callus was
observed after transfer onto MS-callus proliferating (CP) medium. Embryogenic tissue appeared on soft and amorphous callus
and developed into somatic embryos during a subsequent subculture to MS embryo-promoting (EP) media. Various growth regulator
combinations were tested in CP and EP media to obtain a high efficiency of somatic embryo formation. The highest frequency
of calli forming somatic embryos (56.1–68.3%) was observed when CP media with 2.0 or 4.0 mg/l 2,3,5-triiodobenzoic acid were
employed and then cultures were transferred to EP media with 0.5 mg/l 2,4-D and 0.5 mg/l kinetin followed by 0.5 mg/l indole-3-acetic
acid and 0.5 mg/l N6-benzyladenine. Plant development from somatic embryos was obtained on MS medium supplemented with 1.0 mg/l gibberellic acid.
On average, 71.2% of calli displaying somatic embryos converted into plants. Regenerated plants were successfully transferred
to soil. Neither chlorophyll-deficient plants nor morphological variants were found among regenerants. All regenerated plants
were fertile.
Received: 9 May 1997 / Revision received: 25 September 1997 / Accepted: 3 January 1998 相似文献
7.
Tissue culture techniques have been established as a useful approach for ex situ conservation of rare, endemic or threatened
plant species. This report describes the micropropagation of Centaurea paui Loscos ex Willk (Compositae), an extremely endangered plant species endemic to the Valencia Community (eastern Spain), as
a conservation measure which does not cause damage to the wild plants used as explant source. Inflorescence nodal segments
of C. paui were selected as explants for in vitro establishment. The best rate of shoot proliferation was obtained on Murashige and
Skoog (MS) mineral medium supplemented with 0.5 mg/l 6-benzyladenine or with 2 mg/l kinetin. Maximum shoot elongation was
achieved without growth regulators, and the addition of cytokinins significantly decreased their size. In vitro rooting of
shoots was difficult after 6 weeks on rooting media. The combination of 2 mg/l indole-3-acetic acid plus 2 mg/l indole-3-butyric
acid on MS medium yielded the best results. In this medium, 40% of shoots rooted before 30 days of culture. About 70% of the
rooted plants were successfully transferred to pots and acclimatized to ex vitro conditions.
Received: 12 January 1998 / Revision received: 10 October 1998 / Accepted: 28 October 1998 相似文献
8.
A rapid and highly-effective method for micropropagation from nodal segment and shoot tip explants was established for Coleus blumei Benth. Nodal segments and shoot tips were inoculated on MS medium containing 0.7 % agar, 3 % commercial sugar, and different
combinations of 6-benzyladenine (BA) with indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) or α-naphthaleneacetic acid
(NAA). Hundred percent shoot induction from both explants was achieved on the medium containing BA (2 mg dm−3) and NAA (1 mg dm−3). Shoot tips were proved to be the better explant in comparison to nodal segments in having high rate of shoot induction
and more number of shoots. The same media conditions were found suitable for shoot multiplication. Multiplied shoots rooted
best on MS medium supplemented with IBA (2 mg dm−3). Micropropagated plants were successfully established in soil after hardening, with 100 % survival rate. 相似文献
9.
Shoot-tip explants of Rheum emodi Wall. (Polygonaceae) gave rise to multiple shoots when cultured on a Murashige and Skoog (1962) medium (MS) with 2.0 mg/l 6-benzylaminopurine (BAP) and 1.0 mg/l indole-3-butyric acid (IBA). Also, shoot buds developed from leaf explants using MS medium with 2.0 mg/l BAP and 0.25 to 1.0 mg/l indole-3-acetic acid (IAA) or IBA. Roots were induced when the resulting shoots were placed on MS medium with 1.0 mg/l IBA. Both regeneration procedures gave rise to healthy plantlets that were established in soil under glasshouse conditions at 80% frequency after hardening phase of two weeks. Regenerated plants showed a constant chromosome number of 2n=2x=22, same as the parent plant. The use of liquid shake cultures minimized the time and culture medium requirements for propagation. This procedure can be applied for the conservation and utilization of elite clones of R. emodi.Abbreviations BAP
6-benzylaminopurine
- Dd H2O
Double glass distilled water
- IAA
indole-3-acetic acid
- IBA
indole-3-butyric acid
- K
Kinetin
- MS
Murashige and Skoog's (1962) medium
- RH
Relative humidity
CIMAP Publication No. 876 相似文献
10.
Six sugarbeet (Beta vulgaris L.) lines (GWI-248, SPB-11, MonoHy 55, SMS-1, EL45 and FC607) were tested for regeneration. Shoot cultures were initiated in vitro from naked, sterilized embryos obtained from mature seed. Excised petioles from cultured shoots were plated on Gamborg's B5 medium and four modified Murashige and Skoog (MS) media. A medium containing MS inorganic salts supplemented with 0.4 mg/1 N6-benzyladenine, 0.1 mg/1 indole-3-butyric acid, ten vitamins and six amino acids, termed RV, was superior for both adventitious shoot and callus formation. Callus was observed only on RV medium and only on petioles that did not develop adventitious buds directly. Rooting of regenerated shoots and development of complete plants was accomplished by transfer to Gamborg's B5 medium with 5 mg/l indole-3-butyric acid as the sole phytohormone. The complete process of regeneration through adventitious shoot production took from 4 to 6 weeks from explants to rooted plants. The callus that formed on nonorganogenic petioles was regenerative when transferred to fresh RV medium. Regeneration from callus occurred mainly by shoot organogenesis but also by somatic embryogenesis at a low frequency.Abbreviations BA
N6-benzyladenine
- IBA
indole-3-butyric acid
Contribution from Missouri Agricultural Experiment Station. Journal Series No. 10394. University of Missouri, Columbia, MO 63873, USA Mention of trade or company name does not constitute a guarantee or warranty of the product by University of Missouri-Columbia or U.S.D.A. Agricultural Research Service and does not imply their approval to the exclusion of other products that may be suitable. 相似文献
11.
R. Thaniarasu T. Senthil Kumar M. V. Rao 《Physiology and Molecular Biology of Plants》2016,22(1):143-151
The present study describes the plant propagation via indirect organogenesis from in vitro derived leaf and internode explants of Plectranthus bourneae, an endemic plant to south India. Leaf and internodal explants successfully callused on Murashige and Skoog medium (MS) supplemented with different concentrations of auxins [2,4-D (2,4-dichlorophenoxyacetic acid), NAA (α-naphthalene acetic acid), IAA (indole-3 acetic acid), IBA (indole-3-butyric acid) and PIC (Picloram); 0.1–2.0 mg/l] in combination with BA (6-benzyladenine) (0.5 mg/l). Maximum callus induction (98 %) was achieved from leaf explant followed by internodal explant (89 %) at 1.0 mg/l NAA, 0.5 mg/l BA. Leaf derived callus showed better shoot regeneration (29.71 shoots) on MS medium containing 1.0 mg/l KN (kinetin), 0.7 mg/l NAA, and 50 mg/l CH (casein hydrolysate) followed by internodal callus (19.71). A maximum of 19.14 roots/shoot was observed at 1.0 mg/l IBA. The rooted plantlets were successfully hardened and transferred to greenhouse condition with 80 % survival. This system could be utilized for large-scale multiplication of P. bourneae by tissue culture. 相似文献
12.
Catherine Heile-Sudholt Carl A. Huetteman John E. Preece Jerome W. Van Sambeek Gerald R. Gaffney 《Plant Cell, Tissue and Organ Culture》1986,6(2):189-197
Embryonic axes and seedling shoot tips of Juglans nitra L., Black walnut, were cultured in vitro. Significant variation existed among progeny from individual trees for growth of radicles and epicotyls and production of callus and axillary shoots from embryonic axes. The concentration of 6-benzyladenine influenced the growth of the radicle and epicotyl and production of callus and axillary shoots of axes. Axes generally initiated growth quicker on solidified woody plant medium than on Driver and Kuniyuki's walnut medium, but axillary shoot proliferation and elongation were eventually better on liquid Driver and Kuniyuki's walnut medium than on woody plant medium which required an etiolation treatment for microshoot elongation. The concentration of BA also influenced both callus growth and axillary shoot proliferation from seedling shoot tips. Axillary shoots which formed in Driver and Kuniyuki's walnut medium rooted best in sterile vermiculite following a 15 s dip in 10 mM indole-3-butyric acid. Micropropagated plants are growing in the greenhouse. 相似文献
13.
In vitro regeneration of plantlets and multiplication of Sesbania bispinosa (Jacq.) W.F. Wight plants from cultured callus tissue were demonstrated. Callus was established from both cotyledons and mature leaflets on Murashige and Skoog (MS) basal medium supplemented with BAP (0.5 mg/l) and 2,4-D (2 mg/l). Callus mediated shoot bud differentiation was studied under defined nutritional, hormonal and cultural conditions. Various concentrations of BAP or kinetin (Kn) with coconut milk (CM) in MS media induced different levels of shoot bud differentiation as well as multiplication. Multiple shoot bud differentiation occurred in most of the primary calli. The best medium for shoot bud differentiation from cotyledon derived callus, contained BAP (2 mg/l) and 15% CM (V/V). More efficient shoot bud organogenesis was recorded with BAP than Kn. Supplementation with CM in MS media accelerated shoot bud organogenesis in differentiating callus tissue. Rooting of differentiated shoots was achieved by a three step culture procedure involving (a) MS solid medium containing IBA (2 mg/l), (b) growth regulator free half strength MS medium with 1% charcoal, and (c) half strength MS liquid medium free of vitamins, growth regulators and charcoal.Abbreviations IAA
indoleacetic acid
- IBA
indole-3-butyric acid
- NAA
naphthaleneacetic acid
- 2,4-D
2,4-dichlorophenoxyacetic acid
- BAP
6-benzylaminopurine
- Kn
kinetin
- CM
coconut milk
- MS
Murashige and Skoog's medium
- SBI
shoot bud inducing medium 相似文献
14.
A. M. S. Pereira J. R. Moro R. M. M. Cerdeira S. C. França 《Plant Cell, Tissue and Organ Culture》1995,42(3):295-297
Micropropagated shoots of Maytenus ilicifolia Mart. were obtained from axillary buds cultured in Murashige & Skoog medium supplemented with 13.3 M 6-benzyladenine (BA). Addition of 1.1 M 1-indole-3-acetic acid (IAA) to the medium increased shoot elongation. The number of shoots formed was influenced by BA concentration, degree of juvenility of the explant, and by bud explant position on the stem. Cultures of buds taken from stem parts located close to the shoot tip yielded more callus than shoots, whereas axillary buds at distant positions from the apical bud yielded more shoots.Abbreviations BA
6-benzyladenine
- IAA
indole-3-acetic-acid 相似文献
15.
Somatic embryogenesis and subsequent plant regeneration from inflorescence callus of Bambusa beecheyana Munro var. beecheyana 总被引:1,自引:0,他引:1
Somatic embryos of bamboo, Bambusa beecheyana Munro var. beecheyana were developed in callus derived from young florets and adventive roots obtained from floret callus. The medium was a modified Murashige and Skoog medium (1962) supplemented with 3 mg/l 2,4-dichlorophenoxyacetic acid, 2 mg/l kinetin, a high content of sucrose (6%) and 0.7% agar. The embryoids germinated spontaneously to yield whole plantlets on this medium with or without the hormonal adjuvants.Abbreviations 2,4-D
2,4-dichlorophenoxyacetic acid
- IBA
indole-3-butyric acid
- NAA
naphthaleneacetic acid
- MS
Murashige and Skoog's (1962) medium 相似文献
16.
A procedure is outlined for in vitro propagation of two medicinal herbs, Ocimum americanum L. syn. O. canum Sims (hoary basil) and Ocimum sanctum L. (holy basil), using axillary shoot buds. Multiple shoot formation was induced from shoot bud explants of both species on Murashige and Skoog medium (MS) supplemented with benzyladenine (BA). The optimum BA concentrations for shoot proliferation were 0.25 mg/l for O. americanum and 1.0 mg/l for O. sanctum. Incorporation of 0.5 mg/l gibberellic acid (GA3) along with BA in the culture medium resulted in a marked increase in the frequency of axillary branching as well as multiple shoot formation. Shoot buds collected between September through December were most responsive in culture. Shoots of O. americanum were rooted on half-strength MS supplemented with 1.0 mg/l indole-3-butyric acid (IBA), whereas O. sanctum rooted best on medium with 1.0 mg/l naphthaleneacetic acid (NAA). The plantlets were hardened off and successfully established in natural soil, where they grew and matured normally.Abbreviations BA
N6-benzyladenine
- 2,4-D
2,4-dichlorophenoxyacetic acid
- GA3
gibberellic acid
- IAA
indole-3-acetic acid
- IBA
indole-3-butyric acid
- MS
Murashige and Skoog (1962) medium
- NAA
1-naphthaleneacetic acid 相似文献
17.
Summary Factors affecting in vitro shoot production and regeneration of Cercis yunnanensis Hu et Cheng were investigated by comparing various growth regulators and explant types. For optimum shoot production from
axillary buds, Murashige and Skoog (MS) media containing 6-benzyladenine, either alone or in combination with a low concentration
of thidiazuron, resulted in the greatest number of shoots formed per explant (>3). Explants (2 mm long) containing one axillary
bud placed in directcontact with the medium yielded the most shoots per bud (1.6) when grown on growth regulator-free medium.
Root formation on 70–80% of shoot explants was accomplished using either indole-3-butyric acid or α-naphthaleneacetic acid
in the medium, with significantly more roots formed on explants possessing and apical bud than those without the bud. Direct
shoot organogenesis from leaf explants occurred on MS medium containing 10–30 μM thidiazuron, with up to 42% of leaf explants producing shoots. 相似文献
18.
In vitro regeneration of Trifolium glomeratum, a leguminous forage species, was attempted through leaf, petiole, cotyledon, hypocotyl, collar and root explants and two
media combinations. Root and collar explants showed no callus induction. Medium with 0.05 mg dm−3 α-naphthaleneacetic acid (NAA) and 0.10 mg dm−3 N6-benzyladenine (BA) was more effective for hypocotyl explant whereas cotyledon and petiole explant were more responsive to
5.0 mg dm−3 NAA and 1.0 mg dm−3 BA. Friable, green calli obtained from petiole explant on this medium showed organogenetic potential. Modified root-inducing
medium having 0.21 mg dm−3 indole-3-acetic acid and 2.5 % sucrose was successful for root induction and plantlets were successfully transferred to field
after hardening and Rhizobium inoculation. 相似文献
19.
Leaf, stem and root explants of Mandevilla velutina were cultured in vitro and produced vigorous callus in LS basal medium containing one auxin (2,4-D or NAA) plus BAP. Calli can be subcultured indefinitely with vigorous growth. Subculture of calli to NAA (1.0 mg/l) plus BAP (5.0 mg/l) caused profuse regeneration of shoots. Isolated shoots were rooted in basal medium plus NAA (5.0 mg/l) or IBA (8.0 mg/l). Rapidly growing cell suspensions can be easily obtained from friable callus cultured in liquid medium.Abbreviations LS
Linsmaier & Skoog
- 2,4-D
2,4 dichlorophenoxi-acetic acid
- NAA
-naphthalene-acetic acid
- GA3
gibberellic acid
- IAA
indole-3-acetic acid
- BAP
6-benzylaminopurine
- IBA
indole-3-butyric acid 相似文献
20.
Callus was induced on the wounded immature seeds and mature zygotic embryos of Dysosma pleiantha (Hance) Woodson (Berberidaceae) on a medium based on Murashige and Skoog's (1962) formula supplemented with 1 mg/l 2,4-dichlorophenoxy-acetic acid (2,4-D). Spontaneous embryoid formation occurred on the media containing low concentrations of 2,4-D (0.1–0.5 mg/l). These embryoids germinated in either MS or B5 medium containing 1 mg/l N6-benzyladenine and 1 mg/l gibberellic acid. The regenerated plantlets were successfully transferred to soil.Abbreviations 2,4-D
2,4-dichlorophenoxyacetic acid
- BA
N6-benzyladenine
- GA3
gibberellic acid
- MS medium
Murashige and Skoog's (1962) medium 相似文献