辽宁地区普通级和SPF级Wistar大鼠血液生化指标检测

目的建立本省、本地区实验动物生理指标数据背景资料,为科学研究和新药安全性评价提供准确、可靠、科学的参考依据。方法普通级、SPF级Wistar大白鼠（6～8周龄）各60只,雌雄各半,腹主动脉采血,用全自动生化分析仪及配套试剂盒检测血液生化指标。结果普通级Wistar大白鼠的ALT、ALP、CHO、CK、CRE雌雄之间差异显著（P〈0．05或P〈0．01）;SPF级Wistar大白鼠的ALB、ALT、ALP、CHO、BUN、CK、AST、GLU、TBIL雌雄之间差异显著（P〈0．05或P〈0．01）;普通级与SPF级Wistar大白鼠的ALT、ALP、TP、BUN、TBIL、CRE、CHO、CK、AST、ALB、GLU差异显著（P〈0．01）。结论本研究的结果证明普通级与SPF级Wistar大白鼠的血液生化指标差异显著,多数生化指标雌雄之间存在显著差异,为应用提供了有价值的参数。     

引入西藏高原家兔的血清生化指标测定

目的了解和掌握引入西藏高原家兔的生理生化指标,以便为实践教学、临床诊疗和科学研究提供参数。方法采用朗道全自动生化分析仪对引入西藏高原20年的加利福尼亚兔(Californian rabbit)和中国白兔(Chinese white rabbit)的12项血清生化指标进行了测定。结果加利福尼亚兔在品种内比较发现TP、ALB、A/G、CRE、CHOL、LDH指标表现极强的雄兔特征(P0.01),而ALT、GLU测定值雌兔明显高于雄兔(P0.05);中国白兔在品种内比较发现CRE、LDH指标显示差异有显著性(P0.01),CRE指标是雄兔高于雌兔,LDH参数是雌兔高于雄兔。加利福尼亚雄兔与中国白雄兔的参数比较中发现AST、TP、ALB、GLO、A/G、GLU、CRE、LDH指标显示差异有显著性(P0.01),而TG、BUN指标显示差异有显著性(P0.05);加尼福利亚母兔与中国白母兔的参数比较中发现ALT、GLO、CRE、TG指标显示差异有显著性(P0.05),AST、TP、BUN、LDH指标显示差异有显著性(P0.01)。结论实验结果将为西藏高原的教学、临床诊疗和科学研究提供生理数据参考。     

不同因素致亚健康大鼠血液生化、血气电解质指标的影响

目的观察不同因素致亚健康模型大鼠血液生化和血气电解质指标的变化。方法 60只雄性Wistar大鼠随机分为5组,即多因素（MF）组、热水游泳（WS）组、睡眠不足（SD）组、单纯束缚（PC）组和正常对照（C）组,每组12只。采用热水游泳、睡眠剥夺和单纯束缚等单因素或多因素联合建立亚健康大鼠模型,各组造模5d后,处死6只,剩余动物进行恢复性饲养3 d后处死。分别测定造模结束后和恢复3 d时大鼠的血清中的丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、总蛋白（TP）、白蛋白（ALB）、尿素氮（BUN）、肌酐（CREA）、血糖（GLU）、总胆固醇（TC）、甘油三酯（TG）、肌酸磷酸激酶（CK）、乳酸脱氢酶（LDH）和血气电解质等指标。结果 （1）与正常对照（C）组比,造模5d后MF组的大鼠BUN和TG水平显著降低（P〈0.05,P〈0.01）,同时CREA、AST、CK、CK/AST和LDH水平显著升高（P〈0.01）,WS组的大鼠TC、CK、CK/AST和LDH水平亦升高显著（P〈0.01）,且各模型组的大鼠GLU浓度明显增加（P〈0.01）;恢复3 d后,MF组的大鼠TC、AST、ALT、ALB、BUN、CK、CK/AST和LDH水平均明显高于C组（P〈0.05,P〈0.01）;WS组的大鼠血清AST、CK、CK/AST比值和LDH水平亦显著高于C组（P〈0.01）,SD组的大鼠CK显著高于C组（P〈0.05）;（2）与C组比,造模5d后MF组、SD组和PC组的大鼠PCO2、Hct和Hb均明显升高（P〈0.05,P〈0.01）,WS组Hct和Hb升高显著且Na＋显著降低（P〈0.01）,MF组的大鼠PO2和SO2%均显著降低且K＋明显升高（P〈0.05）,且各造模组的大鼠HCO3-♂的浓度均显著升高（P〈0.05,P〈0.01）;恢复3 d后,除各造模大鼠的血液pH值和PO2均明显下降（P〈0.05,P〈0.01）外,MF组、SD组和PC组的大鼠PCO2升高并伴有SO2%的降低（P〈0.05,P〈0.01）,MF组和WS组Na＋仍明显低于C组（P〈0.05,P〈0.01）,SD组的大鼠K＋和Mg2＋则明显高于C组（P〈0.05,P〈0.01）。结论不同因素诱导的亚健康状态大鼠的血液生化、血气电解质指标均出现了不同程度上的改变,故血液生化和血气电解质指标能有效评估亚健康状态机体血液微环境的改变。     

重度子痫前期患者血清CXCL10、CXCL16水平与肝肾功能损害的相关性研究

摘要 目的：探讨重度子痫前期患者血清趋化因子配体（CXCL）10、CXCL16水平与肝肾功能损害的相关性。方法：选取我院于2016年9月至2019年9月收治的轻度子痫前期患者65例（轻度组）、重度子痫前期患者80例（重度组）,另选取同期60例正常妊娠孕妇为对照组,对比各组血清CXCL10、CXCL16、肝功能、肾功能相关指标水平,Pearson相关性分析重度组患者血清CXCL10、CXCL16水平与肝功能、肾功能相关指标间的关系。结果：重度组患者血清CXCL10、CXCL16、丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、谷氨酰转肽酶（GGT）、尿酸（UA）、肌酐（Cr）、尿素氮（BUN）、胱抑素C（Cys-C）水平及24h尿蛋白均高于轻度组和对照组（P＜0.05）,直接胆红素（DBIL）、白蛋白（ALB）、碱性磷酸酶（ALP）、肌酐清除率（CCr）均低于轻度组和对照组（P＜0.05）;轻度组血清CXCL10、CXCL16、ALT、AST、UA、Cr水平及24 h尿蛋白高于对照组（P＜0.05）,ALP和CCr低于对照组（P＜0.05）,轻度组和对照组的ALB、DBIL、GGT、BUN、Cys-C水平比较无差异（P>0.05）。Pearson相关性分析结果显示,重度子痫前期患者CXCL10、CXCL16与ALT、AST、UA、Cr、24h尿蛋白呈正相关（P＜0.05）,与ALP呈负相关（P＜0.05）。结论：血清CXCL10、CXCL16水平升高与重度子痫前期患者肝肾功能改变有关,临床可能通过检测二者水平来辅助评估重度子痫前期患者的病情以便及时予以治疗。     

佳助结扎血管夹对胆囊切除术后兔的安全性研究

目的：观察佳助结扎血管夹对胆囊切除术兔机体的影响,为临床的研究应用提供参考。方法将36只日本大耳白兔随机分成3组,即正常对照组、试验组和同类产品对照（ Hem-o-lok）组,每组12只,除正常对照组外,试验组和同类产品对照组分别用佳助结扎血管夹和Hem-o-lok结扎夹,在开腹胆囊摘除术中夹闭胆囊管及胆囊动脉。观察术后12个月内实验兔的血液生化、电解质、血液学、凝血指标、结扎效果和脏器系数的变化。结果与正常对照组比,试验组兔体重在术后3 d时明显降低（P ＜0.01）,ALT在术后1周时明显升高（P ＜0.05）, CREA和ALB水平均在术后1-2周时显著降低（ P ＜0.05,P ＜0.01）,NEUT数目和TG含量在术后2周时明显升高（P ＜0.05）,GLU在术后1周和1个月时明显降低（P ＜0.01）;LYM和RBC数目分别在术后1个月和1周时降低显著（P ＜0.05）,PLT数目在术后1-2周时均明显升高（P ＜0.01）;同类产品对照组兔体重在术后3 d-1个月时明显降低（P ＜0.05,P ＜0.01）,血浆ALT、NEUT和PLT数目在术后1周时亦明显升高（P ＜0.05,P ＜0.01）,ALB和GLU含量在术后2周时均明显降低（P ＜0.01）,TC含量在术后1-2周时则明显升高（P ＜0.05）;其它时间段,试验组和同类产品对照组兔体重、血液生化指标、血液学参数均与正常对照组比无明显影响（P ＞0.05）;术后各组电解质指标、凝血指标和脏器系数亦均无明显差异（P ＞0.05）。结论佳助结扎血管夹在兔夹闭胆囊管残端和血管中的应用是安全、可靠的,其安全性与Hem-o-lok结扎夹基本一致。     

雌雄金黄地鼠血液生理生化指标的比较分析

目的测定金黄地鼠的血液生理生化参考值,并对两性的参考值进行比较。方法将88只4周龄金黄地鼠,80只8周龄金黄地鼠,采血后,用日本光电MEK-7222K血球分析仪测定血液生理指标,用Olympus AU400全自动生化分析仪测定血清生化指标。结果4周龄金黄地鼠血液生理指标中RBC、HGB、HCT、MCV和RDW性别差异有显著性（P〈0.05）,而WBC、MCH、MCHC、PLT、PCT、MPV和PDW等性别差异无显著性（P〉0.05）;4周龄金黄地鼠血清生化指标中ALB、ALP、GLU、CA、P、CHO和TG性别差异有显著性（P〈0.05）,而ALT、AST、TP、BUN和CREA等性别差异无显著性（P〉0.05）。8周龄金黄地鼠血液生理指标中WBC、LYM、MON、BAS、LYM％、NEUT％、MCH、RBC、HGB、HCT、MCV、PDW和RDW性别差异有显著性（P〈0.05）,而MCHC、PLT、MPV、PCT、NEUT、EOS、BAS、MON％和EOS％等性别差异无显著性（P〉0.05）;8周金黄地鼠血清生化指标中ALT、ALB、BUN、GLU、CREA、P、CHO和TG性别差异有显著性（P〈0.05）,而AST、TP、ALP和CA等性别差异无显著性（P〉0.05）。结论金黄地鼠血液的生理生化值受多种因素影响,部分指标两性问测定值有差异,但多数指标差异不显著。     

超声引导下经皮穿刺聚桂醇注射液与无水乙醇硬化治疗单纯性肝囊肿的疗效对比研究

目的:探讨并对比超声引导下经皮穿刺聚桂醇注射液与无水乙醇硬化治疗单纯性肝囊肿的疗效。方法:选择2014年5月至2016年9月我院收治的60例单纯性肝囊肿患者为研究对象,按随机数字表法分为两组。实验组30例在超声引导下给予聚桂醇注射液进行硬化治疗,对照组30例在超声引导下给予无水乙醇进行硬化治疗。比较两组患者临床有效率、不良反应发生率;于术前及术后24 h检测并对比两组患者血常规指标水平;于术前、术后1周及术后1月检测并对比两组患者总胆红素(TBIL)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)、胆碱酯酶(CHE)及白蛋白(ALB)。结果:实验组总有效率为93.33%,对照组总有效率为90.00%,两组总有效率比较,差异无统计学意义(P0.05)。术前、术后24 h两组各项血常规指标对比,差异均无统计学意义(P0.05)。与术前相比,术后1周实验组患者血清TBIL、ALT、AST、ALP、CHE及ALB均无明显变化(P0.05),对照组患者血清ALT和AST升高(P0.05)。与术后1周相比,术后1个月实验组患者血清ALT、ALP降低,CHE升高(P0.05),对照组ALT、AST及ALP降低,CHE升高(P0.05)。术后1周实验组患者血清ALT和AST水平明显低于对照组(P0.05),TBIL、ALP、CHE及ALB均无明显差异(P0.05);术后1月实验组患者血清ALT水平明显低于对照组,ALB水平明显高于对照组(P0.05),TBIL、AST、ALP及CHE均无明显差异(P0.05)。实验组不良反应发生率为16.67%,低于对照组的30.00%,差异具有统计学意义(P0.05)。结论:超声引导下经皮穿刺聚桂醇注射液与无水乙醇治疗单纯性肝囊肿均具有较好的疗效,但聚桂醇注射液作为硬化剂的不良反应率明显低于无水乙醇,对肝功能的损伤也较无水乙醇小。因此对于单纯性肝囊肿的硬化治疗,聚桂醇注射液是一种安全有效的硬化剂,值得在临床上推广。     

Beagle犬血清与胎牛血清生化特性及其对体外培养的人肺癌细胞生长的影响

目的比较Beagle犬血清与胎牛血清生化特性,探讨它们对体外培养的人肺癌细胞生长的影响。方法收集、制备Beagle犬血清与胎牛血清,采用全自动血液生化分析仪测定23项血清生化指标,比较分析两组血清生化特征差异。取对数期人肺癌细胞QG-56,分别培养于含10%FBS或Beagle-S的RPMI-1640合成培养基中,于第0、24、48、72、96、120和144 h各取出一块培养板进行检测,观察不同血清对细胞生长MTT曲线、细胞形态学的影响。结果FBS中CK、CK-MB、LDH、HBDH、GGT水平明显高于Beagle-S（P〈0.05）;CHE水平却明显低于Beagle-S,两者之间比较,均具有显著统计学意义。血清中的BUN、CRE、GLU、TP、ALB、ALT、ALP、TBIL、DBIL、TG、K^＋、Na^＋、Cl^-、Ca^2＋、Pi^3-水平在Beagle犬与胎牛间无显著性差异。MTT曲线和细胞形态学观察,两组于干预后24、48、72、96、120、144 h对QG-56细胞株生长的影响无显著性差异（P〈0.05）。结论Beagle犬的血清生化特性及其对体外肺癌细胞生长的影响与胎牛相似,可作为血清药理研究的优选动物之一。     

添加鱼油脂肪乳全合一静脉营养对肝功能的影响

目的：了解添加n-3鱼油脂肪乳全合一静脉营养是否可改善肠外营养引起的肝功能损害和胆汁淤积。方法：将100例需要中长期全合一静脉营养支持的患者随机分为2组：对照组50例,使用普通脂肪乳剂250mL;实验组50例,使用普通脂肪乳剂250mL加鱼油脂肪乳剂100mL。输注肠外营养1w后,比较输注前后2组患者血浆转氨酶、胆红素及血浆蛋白水平。结果：肠外营养治疗前后,实验组患者自身对比,血清平均总胆红素（TBIL）、直接胆红素（DBIL）、丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）均明显降低（P＜0.05）。对照组患者自身对比,血清平均ALT、碱性磷酸酶（ALP）、γ-谷氨酰转移酶（γ-GT）均明显升高（P＜0.05）。实验组在肠外营养支持后血清平均TBIL、DBIL、ALT、AST、ALP、γ-GT均明显低于对照组（P＜0.01）。结论：添加n-3鱼油脂肪乳可改善患者的肝功能损害和胆汁淤积。     

血脂康胶囊对冠心病患者的抗氧化及抗炎效果研究

目的：探讨血脂康胶囊对冠心病患者的抗氧化及抗炎效果和意义。方法：选取冠心病并且有一定程度的脂代谢紊乱的患者64例,按随机对照原则随机分为A组（32例）及B组（32例）,A组给予血脂康胶囊600mg,日2次;B组予洛伐他汀40mg/d,丙氨酸转氨酶或天冬氨酸转氨酶升高超过正常值3倍以上的患者服用剂量减半,即洛伐他汀20mg/d,经治疗或者观察恢复正常后再加到40mg/d。共比8周,检测实验前后的血浆ALT,AST,CK,OX-LDL,CRP及SOD水平。结果：血脂康胶囊可以明显的降低血浆TG,CHOL,LDL水平,并且与对照组差异有显著性意义（P〈0.05）。血脂康胶囊可以明显的降低血浆OX-LD,CRP水平,与对照组差异有非常显著性意义（P〈0.01）,血脂康胶囊可以提高血浆SOD水平优于对照组（P〈0.01）,而升高ALT,AST,CK的副作用较对照组低（P〈0.05）。结论：血脂康胶囊常规剂量不仅有效调脂而安全,并且有一定的抗氧化及抗炎效果。     

Bioelement Status with Oral Administration of Fish Oil Methyl Ester and Diesel Fuel in Male Rats

This paper is a study on the effects on the amounts of trace elements in case of possible repeat accidental or environmental exposure with fish oil biodiesel. For this purpose, 35 male Wistar albino rats were used in the study. Rats were divided into five groups. The first group was determined as the control group. The rats in this group were gavaged orally with 250?mg/kg sunflower oil. The rats in the second and third groups were administered by oral gavage of 250?mg/kg (D1) and 500?mg/kg (D2) diesel fuel mixed with equal amounts of sunflower oil, respectively. The rats in the fourth group were administered by oral gavage of 250?mg/kg fish oil biodiesel (F1) and the rats in the fifth group were administered by oral gavage of 500?mg/kg fish oil biodiesel (F2), both mixed with equal amounts of sunflower oil. At the end of the study, bioelement concentrations in the serum and the kidney, lung, and liver tissues were measured using inductively coupled plasma-optical emission spectroscopy. It was observed that serum Ca, Mg, and Sr concentrations were significantly (p?相似文献   

Synergic effects of ultrasound and laser therapies on mesentery for management of obesity and diabetes in rats

Nowadays, the mesentery as a new organ was discovered. Mesenteric events may play an important role in the pathophysiology of several diseases. The aim of our study was investigate the synergic effects of ultrasound (US) and laser therapies on mesentery in obese-hyperglycemic rats. The 25 male Wistar rats were randomized into five groups. Obese nondiabetic (OND) group: obese rats without diabetes and no treatment conditions; SHAM group: obese-hyperglycemic rats treated with placebo; US group: obese-hyperglycemic rats treated with US; LASER group: obese-hyperglycemic rats treated with laser and; US+LASER group: obese-hyperglycemic rats treated with US plus laser. An animal model of type 2 diabetes based on a hyperlipidemic diet combined with a low dose of streptozotocin was used in this study. Body mass and biochemical measurements were performed. GOT and GPT level showed a significant reduction in the treated groups than SHAM. The total cholesterol, triglycerides and very low-density lipoprotein (VLDL) levels showed significantly lower values for the US+LASER group. There was also reduced risk of cardiovascular diseases evidenced by Castelli index in the treated groups than SHAM. This study showed that the US and laser treatment on mesentery resulted to an improvement in biochemical measurements of the obese-hyperglycemic rats, especially the total cholesterol, triglycerides and VLDL levels.     

rhBNP通过抑制细胞凋亡减轻大鼠心肌缺血再灌注损伤

为了研究重组人B型钠尿肽(recombinant human B-type natriuretic peptide, rhBNP)对减轻大鼠心肌缺血再灌注损伤的机制,本研究采用尾部静脉注射的方法对I/R大鼠成功建模,并设计注射生理盐水(I/R组)、rhBNP (I/R+rhBNP组)和假手术组CK组3个处理组,通过TUNEL法检测各处理组大鼠心肌细胞的凋亡情况。本实验还用生理和生化方法检测了心肌细胞中超氧化物歧化酶(superoxidedismutase, SOD)和丙二醛(malondialdehyde, MDA)活性和含量的变化情况,用RT-PCR和免疫印迹方法检测了Bax/Bcl-2信号通路中基因和蛋白表达水平变化。结果表明,rhBNP可以提高I/R大鼠心肌细胞中SOD酶活性,同时使MDA含量降低,表明rhBNP能够保护心肌细胞,使细胞受损程度减小。与此同时本研究发现rhBNP处理后大鼠心肌细胞中Bax基因和蛋白的表达量显著下调,且Bcl-2基因和蛋白的表达显著上调,从而使I/R大鼠心肌细胞的凋亡数目减少,缩小心肌坏死的面积。本研究表明rhBNP可以通过调节Bax/Bcl-2信号通路、提高SOD酶活性使I/R大鼠心肌细胞内MDA含量减少,以及心肌细胞凋亡数目减少,从而有效地减轻大鼠心肌缺血再灌注损伤,以达到保护心肌细胞的目的。     

Effect of Long-term Fluoride Exposure on Lipid Peroxidation and Histology of Testes in First- and Second-generation Rats

This experiment was designed to investigate the histological and lipid peroxidation effects of chronic fluorosis on testes tissues of first- and second-generation rats. Sixteen virgin female Wistar rats were mated with eight males (2:1) for approximately 12 h to obtain first-generation rats. Pregnant rats were divided into two groups: controls and fluoride-given group, each of which containing five rats. Pregnant rats in the fluoride-given group were exposed to a total dose of 30 mg/l sodium fluoride (NaF) in commercial drinking water containing 0.07 mg/l of NaF throughout the gestation and lactation periods. After the lactation period, the young animals (first generation, F1) were exposed to the same dose of NaF in drinking water for 4 months. At the end of the 4 months of experimental period, nine randomly chosen male rats (F1) were killed and testes tissues were taken for histopathological and biochemical analysis. The remaining eight female rats were mated with four males (2:1) for approximately 12 h to obtain second-generation rats. Six female were identified as pregnant and treated with similarly throughout the gestation and the lactation periods. After the lactation period, the young male animals (second generation, F2) were also treated in the same way for 4 months. At the end of the 4 months of experimental period, nine randomly chosen male rats (F2) were killed and testes tissues were collected for histopathological and biochemical analysis. The rats in the control group were applied the same procedure without NaF administration. In biochemical analysis of the fluoride given F1 and F2 rats, it has been found that plasma fluoride levels and testes thiobarbituric acid reactive substance levels were significantly increased when compared with the control group. In F1 and F2 rats, similar histopathological changes were observed. In both groups, spermatogenesis was severely reduced. Spermatogonia and primary spermatocytes were normal, however, there was a widespread degeneration in other spermatogenic cell lines of the seminiferous epithelium. The histological structures of the Sertoli and interstitial Leydig cells were normally observed. It is concluded that chronic fluorosis exposure leads to a remarkable destruction in testes tissues of F1 and F2 rats via lipid peroxidation. The study was carried out in Suleyman Demirel University.     

藏红花提取液对去卵巢大鼠骨密度及骨代谢生化指标的影响

目的：研究中药藏红花提取液对去卵巢大鼠股骨骨密度及血清骨代谢生化指标的影响。方法：选用48只4月龄SD雌性大鼠,随机分为6组：假手术组、模型组、戊酸雌二醇组、藏红花低、中、高剂量组。术后4周各组分别给予相应制剂灌胃,术后12周处死,分别测定股骨骨密度、子宫指数、雌二醇、血钙、血磷、碱性磷酸酶。结果：与模型组相比,藏红花各剂量组股骨骨密度明显升高（p〈0.01）,雌二醇测定值升高（p〈0.01）,碱性磷酸酶显著降低（p〈0.01）,血钙及血磷无统计学差异（p〉0.05）;与戊酸雌二醇组比较,藏红花各剂量组子宫指数显著降低（p〈0.01）。结论：藏红花提取液有助于抑制去卵巢大鼠骨量的丢失,改善骨代谢,对骨质疏松症具有防治作用。     

Myo-Inositol Treatment and GABA-A Receptor Subunit Changes After Kainate-Induced Status Epilepticus

Identification of compounds preventing the biochemical changes that underlie the epileptogenesis process is of great importance. We have previously shown that myo-Inositol (MI) daily treatment prevents certain biochemical changes that are triggered by kainic acid (KA)-induced status epilepticus (SE). The aim of the current work was to study the further influence of MI treatment on the biochemical changes of epileptogenesis and focus on changes in the hippocampus and neocortex of rats for the following GABA-A receptor subunits: α1, α4, γ2, and δ. After SE, one group of rats was treated with saline, while the second group was treated with MI. Control groups that were not treated by the convulsant received either saline or MI administration. 28–30 h after the experiment, a decrease in the amount of the α1 subunit was revealed in the hippocampus and MI had no significant influence on it. On the 28th day of the experiment, the amount of α1 was increased in both the KA? and KA + MI-treated groups. The α4 and γ2 subunits were strongly reduced in the hippocampus of KA-treated animals, but MI significantly halted this reduction. The effects of MI on α4 and γ2 subunit changes were significantly different between hippocampus and neocortex. On the twenty-eighth day after SE, a decrease in the amount of α1 was found in the neocortex, but MI treatment had no effect on it. The obtained results indicate that MI treatment interferes with some of the biochemical processes of epileptogenesis.     

The Effect of Glutathione Treatment on the Biochemical and Immunohistochemical Profile in Streptozotocin-Induced Diabetic Rats

This study investigated the possible role of glutathione (GSH) in diabetic complications and its biochemical safety in experimental diabetic rats. Serum biochemical parameters and the histology of the pancreas were investigated. Seven rats were separated as controls. To create the diabetes in rats, 45 mg/kg single-dose streptozotocin (STZ) was administered i.p. The treatment was continued for 1 month. STZ was administered to the diabetes + GSH group, then reduced GSH, dissolved in isotonic salt solution (200 mg/kg), was applied i.p. two times a week. The GSH group received i.p. GSH. Serum biochemical parameters were determined by autoanalyzer. Immunohistochemical procedures were used to determine the percentage of the insulin-immunoreactive β-cell area in the islets of Langerhans. The biochemical parameters changed to different degrees or did not change. Pancreatic cells of the control and GSH groups were healthy, but in the diabetic and GSH-treated diabetic groups we found damage in different numbers. The results from these analyses show that GSH supplementation can exert beneficial effects on pancreatic cells in STZ-induced diabetic rats and can safely be used for therapy in and protection from diabetes and complications of diabetes.     

Establishment of a biochemically active intestinal ecosystem in ex-germfree rats.

A time course study for the establishment of some biochemical microbial intestinal functions was undertaken in ex-germfree rats conventionalized, i.e., colonized with conventional flora, in three different ways: untreated (group 1); contact with visitor rats (group 2); inoculated with intestinal contents from conventional rats (group 3). The first two groups of rats were inoculated with the intestinal contents from conventional rats after being out of the germfree isolators for 4 weeks. The biochemical parameters studied were degradation of mucin, inactivation of tryptic activity, conversion of cholesterol to coprostanol and of bilirubin to urobilinogen, degradation of beta-aspartylglycine, and formation of short-chain fatty acids. The results showed that the way in which the microbes were introduced and the microbial biochemical functions themselves were of importance. In several cases, social contacts, i.e., contact with visitor rats, were just as effective for the functionally adequate establishment of microbial intestinal functions as was inoculation with intestinal contents from conventional rats. Some of the biochemical parameters studied were established after a few days, whereas the establishment of others was markedly delayed. When inoculated after 4 weeks, all rats in the first two groups were colonized with conventional flora within 1 week. The results indicate that the model system described is suitable when studying buildup mechanisms in intestinal ecosystem(s).     

Establishment of a biochemically active intestinal ecosystem in ex-germfree rats

A time course study for the establishment of some biochemical microbial intestinal functions was undertaken in ex-germfree rats conventionalized, i.e., colonized with conventional flora, in three different ways: untreated (group 1); contact with visitor rats (group 2); inoculated with intestinal contents from conventional rats (group 3). The first two groups of rats were inoculated with the intestinal contents from conventional rats after being out of the germfree isolators for 4 weeks. The biochemical parameters studied were degradation of mucin, inactivation of tryptic activity, conversion of cholesterol to coprostanol and of bilirubin to urobilinogen, degradation of beta-aspartylglycine, and formation of short-chain fatty acids. The results showed that the way in which the microbes were introduced and the microbial biochemical functions themselves were of importance. In several cases, social contacts, i.e., contact with visitor rats, were just as effective for the functionally adequate establishment of microbial intestinal functions as was inoculation with intestinal contents from conventional rats. Some of the biochemical parameters studied were established after a few days, whereas the establishment of others was markedly delayed. When inoculated after 4 weeks, all rats in the first two groups were colonized with conventional flora within 1 week. The results indicate that the model system described is suitable when studying buildup mechanisms in intestinal ecosystem(s).     

Quercetin protection against ciprofloxacin induced liver damage in rats

Ciprofloxacin is a common, broad spectrum antibacterial agent; however, evidence is accumulating that ciprofloxacin may cause liver damage. Quercetin is a free radical scavenger and antioxidant. We investigated histological changes in hepatic tissue of rats caused by ciprofloxacin and the effects of quercetin on these changes using histochemical and biochemical methods. We divided 28 adult female Wistar albino rats into four equal groups: control, quercetin treated, ciprofloxacin treated, and ciprofloxacin + quercetin treated. At the end of the experiment, liver samples were processed for light microscopic examination and biochemical measurements. Sections were prepared and stained with hematoxylin and eosin, and a histopathologic damage score was calculated. The sections from the control group appeared normal. Hemorrhage, inflammatory cell infiltration and intracellular vacuolization were observed in the ciprofloxacin group. The histopathological findings were reduced in the group treated with quercetin. Significant differences were found between the control and ciprofloxacin groups, and between the ciprofloxacin and ciprofloxacin + quercetin groups. Quercetin administration reduced liver injury caused by ciprofloxacin in rats. We suggest that quercetin may be useful for preventing ciprofloxacin induced liver damage.