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1.
Observation of an improved healing process in superficial skin wounds after irradiation with a blue‐LED haemostatic device 下载免费PDF全文
Riccardo Cicchi Francesca Rossi Domenico Alfieri Stefano Bacci Francesca Tatini Gaetano De Siena Gaia Paroli Roberto Pini Francesco S. Pavone 《Journal of biophotonics》2016,9(6):645-655
The healing process of superficial skin wounds treated with a blue‐LED haemostatic device is studied. Four mechanical abrasions are produced on the back of 10 Sprague Dawley rats: two are treated with the blue‐LED device, while the other two are left to naturally recover. Visual observations, non‐linear microscopic imaging, as well as histology and immunofluorescence analyses are performed 8 days after the treatment, demonstrating no adverse reactions neither thermal damages in both abraded areas and surrounding tissue. A faster healing process and a better‐recovered skin morphology are observed: the treated wounds show a reduced inflammatory response and a higher collagen content.
2.
Dual‐polarization Raman spectral imaging to extract overlapping molecular fingerprints of living cells 下载免费PDF全文
Liang‐da Chiu Almar F. Palonpon Nicholas I. Smith Satoshi Kawata Mikiko Sodeoka Katsumasa Fujita 《Journal of biophotonics》2015,8(7):546-554
Raman spectral imaging is gaining more and more attention in biological studies because of its label‐free characteristic. However, the discrimination of overlapping chemical contrasts has been a major challenge. In this study, we introduce an optical method to simultaneously obtain two orthogonally polarized Raman images from a single scan of the sample. We demonstrate how this technique can improve the quality and quantity of the hyperspectral Raman dataset and how the technique is expected to further extend the horizons of Raman spectral imaging in biological studies by providing more detailed chemical information.
3.
Label‐free and non‐invasive monitoring of porcine trophoblast derived cells: differentiation in serum and serum‐free media 下载免费PDF全文
Qifei Li Edison Suasnavas Lifu Xiao Sierra Heywood Xiaojun Qi Anhong Zhou S. Clay Isom 《Journal of biophotonics》2015,8(8):638-645
Traditional approaches to characterize stem cell differentiation are time‐consuming, lengthy and invasive. Here, Raman microspectroscopy (RM) and atomic force microscopy (AFM) – both considered as non‐invasive techniques – are applied to detect the biochemical and biophysical properties of trophoblast derived stem‐like cells incubated up to 10 days under conditions designed to induce differentiation. Significant biochemical and biophysical differences between control cells and differentiated cells were observed. Quantitative real time PCR was also applied to analyze gene expression. The relationship between cell differentiation and associated cellular biochemical and biomechanical changes were discussed.
4.
Mechanisms of renal autoregulation generate oscillations in arterial blood flow at several characteristic frequencies. Full‐field laser speckle flowmetry provides a real‐time imaging of superficial blood microcirculation. The possibility to detect changes in oscillatory dynamics is an important issue in biomedical applications. In this paper we show how laser power density affects quality of the recorded signal and improves detectability of temporal changes in microvascular perfusion.
5.
An infrared sensor analysing label‐free the secondary structure of the Abeta peptide in presence of complex fluids 下载免费PDF全文
Andreas Nabers Julian Ollesch Jonas Schartner Carsten Kötting Just Genius Ute Haußmann Hans Klafki Jens Wiltfang Klaus Gerwert 《Journal of biophotonics》2016,9(3):224-234
The secondary structure change of the Abeta peptide to beta‐sheet was proposed as an early event in Alzheimer's disease. The transition may be used for diagnostics of this disease in an early state. We present an Attenuated Total Reflection (ATR) sensor modified with a specific antibody to extract minute amounts of Abeta peptide out of a complex fluid. Thereby, the Abeta peptide secondary structure was determined in its physiological aqueous environment by FTIR‐difference‐spectroscopy. The presented results open the door for label‐free Alzheimer diagnostics in cerebrospinal fluid or blood. It can be extended to further neurodegenerative diseases.
6.
Erik Bélanger Raphaël Turcotte Amy Daradich Grégory Sadetsky Pierre Gravel Karine Bachand Yves De Koninck Daniel C. Côté 《Journal of biophotonics》2015,8(11-12):884-888
Polarimetric measurements in multiphoton microscopy can reveal information about the local molecular order of a sample. However, the presence of a dichroic through which the excitation beam propagates will generally scramble its polarization. We propose a simple scheme whereby a second properly‐oriented compensation dichroic is used to negate any alteration regardless of the wavelength and the initial polarization. We demonstrate how this robust and rapid approach simplifies polarimetric measurements in second‐harmonic generation, two‐photon excited fluorescence and coherent anti‐Stokes Raman scattering.
7.
Inverse SORS for detecting a low Raman‐active turbid sample placed inside a highly Raman‐active diffusely scattering matrix – A feasibility study 下载免费PDF全文
Khan Mohd. Khan Surjendu B. Dutta Hemant Krishna Shovan K. Majumder 《Journal of biophotonics》2016,9(9):879-887
The broad range of applications of spatially‐offset Raman spectroscopy (SORS) were found to involve samples having only marginal differences in Raman cross‐sections between the surface and subsurface targets. We report the results of a feasibility study to evaluate the potential of the approach to identify the presence of a very low Raman‐active turbid sample placed inside a highly Raman‐active diffusely scattering matrix. Paraffin sandwiched tissue blocks prepared by embedding slices of chicken muscle tissue into solid paraffin blocks were employed as representative samples for the study. It was found that in contrast to the several millimetres of probing depth reported in the earlier applications, the Raman signatures of tissue were best recovered when it was located beneath the surface of the paraffin block at a depth of around a millimetre, beyond which the quality of recovery was increasingly poorer. However, the probing depth could be further increased by increasing the thickness of the embedded tissue sections. The results clearly suggest that though the probing depth achievable under the current condition is less than that found in previous applications, nevertheless it is sufficient for various other applications that would not require probing as deep as was required earlier.
8.
Pejhman Ghassemi Lauren T. Moffatt Jeffrey W. Shupp Jessica C. Ramella‐Roman 《Journal of biophotonics》2016,9(1-2):100-108
A study of polarized light transport in scattering media exhibiting directional anisotropy or linear birefringence is presented in this paper. Novel theoretical and experimental methodologies for the quantification of birefringent alignment based on out‐of‐plane polarized light transport are presented here. A polarized Monte Carlo model and a polarimetric imaging system were devised to predict and measure the impact of birefringence on an impinging linearly polarized light beam. Ex‐vivo experiments conducted on bovine tendon, a biological sample consisting of highly packed type I collagen fibers with birefringent property, showed good agreement with the analytical results.
9.
Synergistic effects of atmospheric pressure plasma‐emitted components on DNA oligomers: a Raman spectroscopic study 下载免费PDF全文
Eugen Edengeiser Jan‐Wilm Lackmann Erik Bründermann Simon Schneider Jan Benedikt Julia E. Bandow Martina Havenith 《Journal of biophotonics》2015,8(11-12):918-924
Cold atmospheric‐pressure plasmas have become of increasing importance in sterilization processes especially with the growing prevalence of multi‐resistant bacteria. Albeit the potential for technological application is obvious, much less is known about the molecular mechanisms underlying bacterial inactivation. X‐jet technology separates plasma‐generated reactive particles and photons, thus allowing the investigation of their individual and joint effects on DNA. Raman spectroscopy shows that particles and photons cause different modifications in DNA single and double strands. The treatment with the combination of particles and photons does not only result in cumulative, but in synergistic effects. Profilometry confirms that etching is a minor contributor to the observed DNA damage in vitro.
10.
Robert Koprowski 《Journal of biophotonics》2015,8(11-12):935-943
The paper presents problems and solutions related to hyperspectral image pre‐processing. New methods of preliminary image analysis are proposed. The paper shows problems occurring in Matlab when trying to analyse this type of images. Moreover, new methods are discussed which provide the source code in Matlab that can be used in practice without any licensing restrictions.
11.
Luca Dalbosco Giulia Zanini Elvira D'Amato Francesco Tessarolo Sebastiana Boi Paolo Bauer Albrecht Haase Renzo Antolini 《Journal of biophotonics》2015,8(10):816-825
Photodamage, induced by femtosecond laser radiation, was studied in thick samples of human skin tissue (healthy skin and neoplastic lesions). Photobleaching, photoionization, and thermomechanical damage effects were characterized comparatively. The laser power dependence of the damage rates allowed to connect macroscopic effects to underlying molecular processes. Optical effects were correlated to histopathological changes. Tissue alterations were found only from thermomechanical cavitation and limited to superficial layers of the epidermis. From the depth‐dependencies of all damage thresholds a depth‐dependent power‐compensation scheme was defined allowing for damage‐free deep tissue optical biopsy.
12.
Back Cover: Non‐invasive optical method for real‐time assessment of intracorneal riboflavin concentration and efficacy of corneal cross‐linking (J. Biophotonics 7/2018) 下载免费PDF全文
Giuseppe Lombardo Valentina Villari Norberto L. Micali Nancy Leone Cristina Labate Maria P. De Santo Marco Lombardo 《Journal of biophotonics》2018,11(7)
We disclose a theranostic device for performing image‐guided riboflavin/UV‐A corneal cross‐linking. The device determines treatment efficacy by real time monitoring of riboflavin concentration in the corneal stroma. The study shows efficacy of the device in eye bank human donor tissues. Further details can be found in the article by Giuseppe Lombardo et al. ( e201800028 )
13.
Earu Banoth Vamshi Krishna Kasula Veerendra Kalyan Jagannadh Sai Siva Gorthi 《Journal of biophotonics》2016,9(6):610-618
In this work, an optofluidic flow analyzer, which can be used to perform malaria diagnosis at the point‐of‐care is demonstrated. The presented technique is based on quantitative optical absorption measurements carried out on a single cell level for a given population of Human Red Blood Cells (RBCs). By measuring the optical absorption of each RBC, the decrease in the Hemoglobin (Hb) concentration in the cytoplasm of the cell due to the invasion of malarial parasite is detected. Cells are assessed on a single cell basis, as they pass through a microfluidic channel. The proposed technique has been implemented with inexpensive off‐the‐shelf components like laser diode, photo‐detector and a micro‐controller. The ability of the optofluidic flow analyzer to asses about 308,049 cells within 3 minutes has been demonstrated. The presented technique is capable of detecting very low parasitemia levels with high sensitivity.
14.
Farshid Bahrami Mathieu Maisonneuve Michel Meunier Arthur O. Montazeri Yujin Kim Nazir P. Kherani J. Stewart Aitchison Mo Mojahedi 《Journal of biophotonics》2017,10(2):271-277
A plasmon waveguide resonance (PWR) sensor is proposed for studying the interaction between gold nanoparticles and proteins. The ability of the PWR sensor to operate in both TM and TE Polarizations, i.e. its polarization diversity, facilitates the simultaneous spectroscopy of the nanoparticles surface reactions using both polarizations. The response of each polarization to streptavidin‐biotin binding at the surface of gold nanoparticles is investigated in real time. Finally, using the principles of multimode spectroscopy, the nanoparticle's surface reactions are decoupled from the bulk solution refractive index variations.
15.
Subcellular measurements of mechanical and chemical properties using dual Raman‐Brillouin microspectroscopy 下载免费PDF全文
Zhaokai Meng Sandra C. Bustamante Lopez Kenith E. Meissner Vladislav V. Yakovlev 《Journal of biophotonics》2016,9(3):201-207
Brillouin microspectroscopy is a powerful technique for noninvasive optical imaging. In particular, Brillouin microspectroscopy uniquely allows assessing a sample's mechanical properties with microscopic spatial resolution. Recent advances in background‐free Brillouin microspectroscopy make it possible to image scattering samples without substantial degradation of the data quality. However, measurements at the cellular‐ and subcellular‐level have never been performed to date due to the limited signal strength. In this report, by adopting our recently optimized VIPA‐based Brillouin spectrometer, we probed the microscopic viscoelasticity of individual red blood cells. These measurements were supplemented by chemically specific measurements using Raman microspectroscopy.
16.
Inside Back Cover: The conformation of bovine serum albumin adsorbed to the surface of single all‐dielectric nanoparticles following light‐induced heating (J. Biophotonics 7/2018) 下载免费PDF全文
Andrei A. Krasilin Katerina Volodina Arina A. Sukhova Mihail I. Petrov Dmitry A. Zuev Vyacheslav A. Dyachuk Valentin A. Milichko 《Journal of biophotonics》2018,11(7)
Germanium vs Silicon: All‐dielectric nanoparticles provides the heat resistance for proteins under light‐induced heating. Further details can be found in the article by Andrei A. Krasilin et al. ( e201700322 )
17.
Label‐free,zeptomole cancer biomarker detection by surface‐enhanced fluorescence on nanoporous gold disk plasmonic nanoparticles 下载免费PDF全文
Greggy M. Santos Fusheng Zhao Jianbo Zeng Ming Li Wei‐Chuan Shih 《Journal of biophotonics》2015,8(10):855-863
We experimentally demonstrate a label‐free biosensor for the ERBB2 cancer gene DNA target based on the distance‐dependent detection of surface‐enhanced fluorescence (SEF) on nanoporous gold disk (NPGD) plasmonic nanoparticles. We achieve detection of 2.4 zeptomole of DNA target on the NPGD substrate with an upper concentration detection limit of 1 nM. Without the use of molecular spacers, the NPGD substrate as an SEF platform was shown to provide higher net fluorescence for visible and NIR fluorophores compared to glass and non‐porous gold substrates. The enhanced fluorescence signals in patterned nanoporous gold nanoparticles make NPGD a viable material for further reducing detection limits for biomolecular targets used in clinical assays.
18.
Significance of the follicular pathway for dermal substance penetration quantified by laser Doppler flowmetry 下载免费PDF全文
Staffan Vandersee Ute Erdmenger Alexa Patzelt Marc Beyer Martina C. Meinke Maxim E. Darvin Jürgen Koscielny Jürgen Lademann 《Journal of biophotonics》2016,9(3):276-281
The understanding of transdermal substance penetration pathways remains an important field for the development of future topical drugs and cosmetics. Laser Doppler flowmetry is a well‐established method for evaluating cutaneous perfusion. In a study on 6 healthy male volunteers, we topically applied the vasoactive substance benzyl nicotinate on two test areas with open and obturated hair follicles and measured changes in the blood flow by Doppler flowmetry. Contrary to occluded follicles, the application onto the test area with open follicles led to a statistically significant perfusion increase within the first 5 minutes, emphasizing the importance of the follicular pathway for epidermal penetration.
19.
Multi‐modal acousto‐optic/ultrasound imaging of ex vivo liver tumors at 790 nm using a Sn2P2S6 wavefront adaptive holographic setup 下载免费PDF全文
Jean‐Baptiste Laudereau Emilie Benoit à La Guillaume Vincent Servois Pascale Mariani Alexander A. Grabar Mickaël Tanter Jean‐Luc Gennisson François Ramaz 《Journal of biophotonics》2015,8(5):429-436
Biological tissues are very strong light‐scattering media. As a consequence, current medical imaging devices do not allow deep optical imaging unless invasive techniques are used. Acousto‐optic imaging is a light‐ultrasound coupling technique that takes advantage of the ballistic propagation of ultrasound in biological tissues to access optical contrast with a millimeter resolution. We have developed a photorefractive‐crystal‐based system that performs self‐adaptive wavefront holography and works within the optical therapeutic window. As it works at an appropriate wavelength range for biological tissues imaging, it was tested on ex vivo liver samples containing tumors as a pre‐clinical study. Optical contrast was obtained even if acoustical one was not significant.
20.
Rapid and label‐free detection and assessment of bacteria by terahertz time‐domain spectroscopy 下载免费PDF全文
Shihan Yan Yueping Liu Shu Yu Mingkun Zhang Zhongbo Yang Xiaoyan Zhu Qing Huang Hong‐Liang Cui Weiling Fu 《Journal of biophotonics》2016,9(10):1050-1058
Here we demonstrated the potential and applicability of terahertz (THz) spectroscopy to detect four commonly found bacteria in the infectious diseases. Besides the different spectral characteristics between bacterial species, THz absorption differences for living bacteria, dead bacteria and bacterial powder of the same species were also investigated. Our results revealed that small differences in water contents between bacterial cells account for distinct discrepancies of the absorption coefficients, which can be used for bacterial species identification. Furthermore, living and dead bacteria showed different absorption coefficients as a result of their different hydration levels, suggesting that THz spectroscopy can be used to rapidly assess the living state of bacteria under test. Our results clearly demonstrated the ability of THz spectroscopy for time‐saving and label‐free detection of bacteria with minimal sample preparation, potentially to be utilized for point‐of‐care tests in the near future.