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1.
Quantitative trait loci for yield and related traits in the wheat population Ning7840 × Clark 总被引:1,自引:0,他引:1
Marza F Bai GH Carver BF Zhou WC 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(4):688-698
Grain yield and associated agronomic traits are important factors in wheat (Triticum aestivum L.) improvement. Knowledge regarding the number, genomic location, and effect of quantitative trait loci (QTL) would facilitate
marker-assisted selection and the development of cultivars with desirable characteristics. Our objectives were to identify
QTLs directly and indirectly affecting grain yield expression. A population of 132 F12 recombinant inbred lines (RILs) was derived by single-seed descent from a cross between the Chinese facultative wheat Ning7840
and the US soft red winter wheat Clark. Phenotypic data were collected for 15 yield and other agronomic traits in the RILs
and parental lines from three locations in Oklahoma from 2001 to 2003. Twenty-nine linkage groups, consisting of 363 AFLP
and 47 SSR markers, were identified. Using composite interval mapping (CIM) analysis, 10, 16, 30, and 14 QTLs were detected
for yield, yield components, plant adaptation (shattering and lodging resistance, heading date, and plant height), and spike
morphology traits, respectively. The QTL effects ranged from 7 to 23%. Marker alleles from Clark were associated with a positive
effect for the majority of QTLs for yield and yield components, but gene dispersion was the rule rather than the exception
for this RIL population. Often, QTLs were detected in proximal positions for different traits. Consistent, co-localized QTLs
were identified in linkage groups 1AL, 1B, 4B, 5A, 6A, and 7A, and less consistent but unique QTLs were identified on 2BL,
2BS, 2DL, and 6B. Results of this study provide a benchmark for future efforts on QTL identification for yield traits. 相似文献
2.
Hori K Sato K Takeda K 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,115(6):869-876
Seed dormancy is one of the most important traits in germination process to control malting and pre-harvest sprouting in barley
(Hordeum vulgare L.). EST based linkage maps were constructed on seven recombinant inbred (RI) and one doubled haploid (DH) populations derived
from crosses including eleven cultivated and one wild barley strains showing the wide range of seed dormancy levels. Seed
dormancy of each RI and DH line was estimated from the germination percentage at 5 and 10 weeks post-harvest after-ripening
periods in 2003 and 2005. Quantitative trait loci (QTLs) controlling seed dormancy were detected by the composite interval
mapping procedure on the RI and DH populations. A total of 38 QTLs clustered around 11 regions were identified on the barley
chromosomes except 2H among the eight populations. Several QTL regions detected in the present study were reported on similar
positions in the previous QTL studies. The QTL on at the centromeric region of long arm of chromosome 5H was identified in
all the RI and DH populations with the different degrees of dormancy depth and period. The responsible gene of the QTL might
possess a large allelic variation among the cross combinations, or can be multiple genes located on the same region. The various
loci and their different effects in dormancy found in the barley germplasm in the present study enable us to control the practical
level of seed dormancy in barley breeding programs.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
3.
Chloupek O Forster BP Thomas WT 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(5):779-786
Root system size (RSS) was measured in 12 diverse barley genotypes and 157 double-haploid lines (DHs), using electric capacitance.
The parents of the DHs, Derkado and B83-12/21/5, carry different semi-dwarfing genes, sdw1 and ari-e.GP, respectively. Estimates of RSS were taken in the field thrice during plant development: stem elongation (RSS1), heading
(RSS2) and grain filling (RSS3). The 12 barley genotypes were assessed over 3 years and at two or three locations each year;
the DH mapping population was assessed at two locations in 2002. Among the 12 barley genotypes, those with the semi-dwarf
genes had greater RSS values in all 3 years (28.9, 24.6 and 15.0% in years 1, 2 and 3, respectively) compared to non-semi-dwarf
controls. The DH population showed transgressive segregation on both sides of the parent means, indicating polygenic control
of RSS. Quantitative trait loci (QTLs) for RSS were found on five of the seven chromosomes: 1H, 3H, 4H, 5H and 7H and these
were compared with previously mapped agronomic traits. The TotalRSS QTL on 3H was associated with sdw1 and QTLs for height, plant yield and plant weight. The RSS3 QTL on 5H was associated with ari-e.GP and QTLs for height, plant yield, plant weight, harvest index and tiller number. The RSS3 QTL on 7H was also associated
with a TotalRSS QTL and QTLs for plant weight and harvest index. Other RSS QTLs were not associated with any other trait studied.
RSS is considered to be a polygenic trait linked to important traits, in particular to yield. The study highlights the effects
of semi-dwarfing genes and discusses the potential for breeding for root traits. 相似文献
4.
Yong-Bi Fu Gregory W. Peterson Brent D. McCallum Li Huang 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,120(2):271-281
Little is known about the genetic control of heterosis in the complex polyploid crop species oilseed rape (Brassica napus L.). In this study, two large doubled-haploid (DH) mapping populations and two corresponding sets of backcrossed test hybrids
(THs) were analysed in controlled greenhouse experiments and extensive field trials for seedling biomass and yield performance
traits, respectively. Genetic maps from the two populations, aligned with the help of common simple sequence repeat markers,
were used to localise and compare quantitative trait loci (QTL) related to the expression of heterosis for seedling developmental
traits, plant height at flowering, thousand seed mass, seeds per silique, siliques per unit area and seed yield. QTL were
mapped using data from the respective DH populations, their corresponding TH populations and from mid-parent heterosis (MPH)
data, allowing additive and dominance effects along with digenic epistatic interactions to be estimated. A number of genome
regions containing numerous heterosis-related QTL involved in different traits and at different developmental stages were
identified at corresponding map positions in the two populations. The co-localisation of per se QTL from the DH population
datasets with heterosis-related QTL from the MPH data could indicate regulatory loci that may also contribute to fixed heterosis
in the highly duplicated B. napus genome. Given the key role of epistatic interactions in the expression of heterosis in oilseed rape, these QTL hotspots might
harbour genes involved in regulation of heterosis (including fixed heterosis) for different traits throughout the plant life
cycle, including a significant overall influence on heterosis for seed yield. 相似文献
5.
6.
Genetic analysis of grain protein-content,grain yield and thousand-kernel weight in bread wheat 总被引:21,自引:0,他引:21
Groos C Robert N Bervas E Charmet G 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2003,106(6):1032-1040
Grain yield and grain protein content are two very important traits in bread wheat. They are controlled by genetic factors, but environmental conditions considerably affect their expression. The aim of this study was to determine the genetic basis of these two traits by analysis of a segregating population of 194 F(7) recombinant inbred lines derived from a cross between two wheat varieties, grown at six locations in France in 1999. A genetic map of 254 loci was constructed, covering about 75% of the bread wheat genome. QTLs were detected for grain protein-content (GPC), yield and thousand-kernel weight (TKW). 'Stable' QTLs (i.e. detected in at least four of the six locations) were identified for grain protein-content on chromosomes 2A, 3A, 4D and 7D, each explaining about 10% of the phenotypic variation of GPC. For yield, only one important QTL was found on chromosome 7D, explaining up to 15.7% of the phenotypic variation. For TKW, three QTLs were detected on chromosomes 2B, 5B and 7A for all environments. No negative relationships between QTLs for yield and GPC were observed. Factorial Regression on GxE interaction allowed determination of some genetic regions involved in the differential reaction of genotypes to specific climatic factors, such as mean temperature and the number of days with a maximum temperature above 25 degrees C during grain filling. 相似文献
7.
Molecular detection of QTLs for agronomic and quality traits in a doubled haploid population derived from two Canadian wheats (Triticum aestivum L.) 总被引:13,自引:0,他引:13
Huang XQ Cloutier S Lycar L Radovanovic N Humphreys DG Noll JS Somers DJ Brown PD 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,113(4):753-766
Development of high-yielding wheat varieties with good end-use quality has always been a major concern for wheat breeders. To genetically dissect quantitative trait loci (QTLs) for yield-related traits such as grain yield, plant height, maturity, lodging, test weight and thousand-grain weight, and for quality traits such as grain and flour protein content, gluten strength as evaluated by mixograph and SDS sedimentation volume, an F1-derived doubled haploid (DH) population of 185 individuals was developed from a cross between a Canadian wheat variety “AC Karma” and a breeding line 87E03-S2B1. A genetic map was constructed based on 167 marker loci, consisting of 160 microsatellite loci, three HMW glutenin subunit loci: Glu-A1, Glu-B1 and Glu-D1, and four STS-PCR markers. Data for investigated traits were collected from three to four environments in Manitoba, Canada. QTL analyses were performed using composite interval mapping. A total of 50 QTLs were detected, 24 for agronomic traits and 26 for quality-related traits. Many QTLs for correlated traits were mapped in the same genomic regions forming QTL clusters. The largest QTL clusters, consisting of up to nine QTLs, were found on chromosomes 1D and 4D. HMW glutenin subunits at Glu-1 loci had the largest effect on breadmaking quality; however, other genomic regions also contributed genetically to breadmaking quality. QTLs detected in the present study are compared with other QTL analyses in wheat. 相似文献
8.
Genetic dissection of interactions between wheat flour starch and its components in two populations using two QTL mapping methods 总被引:1,自引:0,他引:1
Zhiying Deng Wenqi Fang Xin Guo Xinying Zhao Hong Guo Shuna Hu Jichun Tian 《Molecular breeding : new strategies in plant improvement》2018,38(4):41
Starch content and its components are important for determining wheat end-use quality and yield. However, little information is available about their interactions at the QTL/gene level in more than one population using different QTL mapping methods. Therefore, to dissect these interactions, two mapping populations from two locations over 2 years were used. The QTLs for the populations were analyzed by unconditional and conditional QTL mapping by two different analysis methods. In the two populations, there were a total of 24 unconditional additive QTLs detected for flour amylose (FAMS), flour amylopectin (FAMP), flour total starch (FTSC), and the ratio of FAMS to FAMP using ICIMapping4.1 methods, but 26 unconditional QTLs were found using QTLNetwork2.0 methods. Of these QTLs, 10 stable major additive QTLs were identified in more than one environment, mainly distributed on chromosomes 3B, 4A, 5A, and 7D. The maximum percentage of phenotypic variance explained (PVE) reached 54.31%. Two new unconditional major additive QTLs on chromosome 3B (Qftsc3B and Qfamp3B) were found. A total of 23 and 19 conditional additive QTLs were identified in the two populations using two different methods, respectively. Of which, eight and six stable major conditional QTLs were detected on chromosomes 3B, 4A, and 7D, respectively. New repressed QTLs were identified, such as Qftsc/fams5B-1 and Qftsc/fams5B-2. There were 20 epistatic unconditional and 15 conditional QTLs detected. In all, important QTLs on chromosomes 3B, 4A, and 7A were found in both populations. However, the number of important QTLs in the special recombinant inbred line (RIL) population was higher than that in the double haploid (DH) population, especially on chromosomes 7D and 5B. Moreover, the QTLs on chromosomes 4A, 7A, and 7D were close to the Wx-1 loci in the RIL population. These indicated better results can be obtained by a special population to target traits than by a common population. The important QTLs on key chromosomes can always be detected no matter what kinds of populations are used, such as the QTLs on chromosome 4A. In addition, QTL clusters were found on chromosomes 4A, 3B, 7A, 7D, and 5A in the two populations, indicating these chromosome regions were very important for starch biosynthesis. 相似文献
9.
Sibov ST de Souza CL Garcia AA Silva AR Garcia AF Mangolin CA Benchimol LL de Souza AP 《Hereditas》2003,139(2):107-115
A previous genetic map containing 117 microsatellite loci and 400 F(2) plants was used for quantitative trait loci (QTL) mapping in tropical maize. QTL were characterized in a population of 400 F(2:3) lines, derived from selfing the F(2) plants, and were evaluated with two replications in five environments. QTL determinations were made from the mean of these five environments. Grain yield (GY), plant height (PH), ear height (EH) and grain moisture (GM) were measured. Variance components for genotypes (G), environments (E) and GxE interaction were highly significant for all traits. Heritability was 0.69 for GY, 0.66 for PH, 0.67 for EH and 0.23 for GM. Using composite interval mapping (CIM), a total of 13 distinct QTLs were identified: four for GY, four for PH and five for EH. No QTL was detected for GM. The QTL explained 32.73 % of the phenotypic variance of GY, 24.76 % of PH and 20.91 % of EH. The 13 QTLs displayed mostly partial dominance or overdominance gene action and mapped to chromosomes 1, 2, 7, 8 and 9. Most QTL alleles conferring high values for the traits came from line L-14-4B. Mapping analysis identified genomic regions associated with two or more traits in a manner that was consistent with correlation among traits, supporting either pleiotropy or tight linkage among QTL. The low number of QTLs found, can be due to the great variation that exists among tropical environments. 相似文献
10.
水稻穗部性状的QTL与环境互作分析 总被引:31,自引:3,他引:28
分别在两年收集珍汕97/明恢63的重组自交系群体的表现数据,运用混合线性模型的QTL定位方法,联合分析穗部5个性状的QTLs7及QTL与环境互作关系。每穗颖花数、每穗实粒数、结实率、穗长和穗着密度分别检测到10、3、6、8和7个QTLs分别解释各性状变异的29.13%、19.2%、29.46%、26.39%和35.76%。对于同一性状,高值亲本和低值亲本中均存在增效和减效QTL。相关性状QTL的位置表现相同或相似,高值亲本和低值亲本中均存在增效和减效QTL。相关性状QTL的位置表现相同或相似,成族分布。1个穗长QTL,2个每穗颖花数QTL3,3个结实率QTLs表现与环境显著互作,QTL与环境互作效应的贡献率比相应的QTL贡献率略大。遗传力稍高的每穗实粒数和穗着粒密度的DQTL与环境不互作。 相似文献
11.
Agricultural environments deteriorate due to excess nitrogen application.Breeding for low nitrogen responsive genotypes can reduce soil nitrogen input.Rice genotypes respond variably to soil available nitrogen.The present study attempted quantification of genotype x nitrogen level interaction and mapping of quantitative trait loci (QTLs) associated with nitrogen use efficiency (NUE) and other associated agronomic traits.Twelve parameters were observed across a set of 82 double haploid (DH) lines derived from IR64/Azucena.Three nitrogen regimes namely,native (0 kg/ha; no nitrogen applied),optimum (100 kg/ha) and high (200 kg/ha) replicated thrice were the environments.The parents and DH lines were significantly varying for all traits under different nitrogen regimes.All traits except plant height recorded significant genotype x environment interaction.Individual plant yield was positively correlated with nitrogen use efficiency and nitrogen uptake.Sixteen QTLs were detected by composite interval mapping.Eleven QTLs showed significant QTL x environment interactions.On chromosome 3,seven QTLs were detected associated with nitrogen use,plant yield and associated traits.A QTL region between markers RZ678,RZ574 and RZ284 was associated with nitrogen use and yield.This chromosomal region was enriched with expressed gene sequences of known key nitrogen assimilation genes. 相似文献
12.
Ghaffary SM Robert O Laurent V Lonnet P Margalé E van der Lee TA Visser RG Kema GH 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,123(5):741-754
The ascomycete Mycosphaerella graminicola is the causal agent of septoria tritici blotch (STB), one of the most destructive foliar diseases of bread and durum wheat
globally, particularly in temperate humid areas. A screening of the French bread wheat cultivars Apache and Balance with 30
M. graminicola isolates revealed a pattern of resistant responses that suggested the presence of new genes for STB resistance. Quantitative
trait loci (QTL) analysis of a doubled haploid (DH) population with five M. graminicola isolates in the seedling stage identified four QTLs on chromosomes 3AS, 1BS, 6DS and 7DS, and occasionally on 7DL. The QTL
on chromosome 6DS flanked by SSR markers Xgpw5176 and Xgpw3087 is a novel QTL that now can be designated as Stb18. The QTLs on chromosomes 3AS and 1BS most likely represent Stb6 and Stb11, respectively, and the QTLs on chromosome 7DS are most probably identical with Stb4 and Stb5. However, the QTL identified on chromosome 7DL is expected to be a new Stb gene that still needs further characterization. Multiple isolates were used and show that not all isolates identify all QTLs,
which clearly demonstrates the specificity in the M. graminicola–wheat pathosystem. QTL analyses were performed with various disease parameters. The development of asexual fructifications
(pycnidia) in the characteristic necrotic blotches of STB, designated as parameter P, identified the maximum number of QTLs. All other parameters identified fewer but not different QTLs. The segregation of
multiple QTLs in the Apache/Balance DH population enabled the identification of DH lines with single QTLs and multiple QTL
combinations. Analyses of the marker data of these DH lines clearly demonstrated the positive effect of pyramiding QTLs to
broaden resistance spectra as well as epistatic and additive interactions between these QTLs. Phenotyping of the Apache/Balance
DH population in the field confirmed the presence of the QTLs that were identified in the seedling stage, but Stb18 was inconsistently expressed and might be particularly effective in young plants. In contrast, an additional QTL for STB
resistance was identified on chromosome 2DS that is exclusively and consistently expressed in mature plants over locations
and time, but it was also strongly related with earliness, tallness as well as resistance to Fusarium head blight. Although
to date no Stb gene has been reported on chromosome 2D, the data provide evidence that this QTL is only indirectly related to STB resistance.
This study shows that detailed genetic analysis of contemporary commercial bread wheat cultivars can unveil novel Stb genes that can be readily applied in marker-assisted breeding programs. 相似文献
13.
Chaïb J Lecomte L Buret M Causse M 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(5):934-944
The efficiency of marker-assisted backcross for the introgression of a quantitative trait locus (QTL) from a donor line into
a recipient line depends on the stability of QTL expression. QTLs for six quality traits in tomato (fruit weight, firmness,
locule number, soluble solid content, sugar content and titratable acidity) were studied in order to investigate their individual
effect and their stability over years, generations and genetic backgrounds. Five chromosome regions carrying fruit quality
QTLs were transferred following a marker-assisted backcross scheme from a cherry tomato line into three modern lines with
larger fruits. Three sets of genotypes corresponding to three generations were compared: (1) an RIL population, which contained
50% of each parental genome, (2) three BC3S1 populations which segregated simultaneously for the five regions of interest
but were almost fully homozygous for the recipient genome on the eight chromosomes carrying no QTL and (3) three sets of QTL-NILs
(BC3S3 lines) which differed from the recipient line only in one of the five regions. QTL detection was performed in each
generation, in each genetic background and during 2 successive years for QTL-NILs. About half of the QTLs detected in QTL-NILs
were detected in both years. Eight of the ten QTLs detected in RILs were recovered in the QTL-NILs with the genetic background
used for the initial QTL mapping experiment, with the exception of two QTLs for fruit firmness. Several new QTLs were detected.
In the two other genetic backgrounds, the number of QTLs in common with the RILs was lower, but several new QTLs were also
detected in advanced generations. 相似文献
14.
Detection of QTLs for heading time and photoperiod response in wheat using a doubled-haploid population. 总被引:16,自引:0,他引:16
The genetic basis of heading time in wheat (Triticum aestivum L.) was investigated through the study of flowering under normal autumn sown field conditions as well as photoperiod responses under a controlled environment. Quantitative trait loci (QTLs) for these traits were mapped in a doubled-haploid (DH) population derived from a cross between the wheat cultivars 'Courtot' and 'Chinese Spring'. A molecular marker linkage map of this cross that was previously constructed based on 187 DH lines and 380 markers was used for QTL mapping. The genome was well covered (85%) except for chromosomes 1D and 4D, and a set of anchor loci regularly spaced over the genome (one marker each 15.5 cM) was chosen for marker regression analysis. The presence of a QTL was declared at a significance threshold of alpha = 0.005. The population was grown under field conditions in Clermont-Ferrand, France during two years (1994-1995), in Norwich, U.K. over one year (1998), and also under controlled environments in Norwich. For each trait, between 2 and 4 QTLs were identified with individual effects ranging between 6.3% and 44.4% of the total phenotypic variation. Two QTLs were detected that simultaneously affected heading time and photoperiod response. For heading time, these two QTLs were detected in more than one year. One QTL located on chromosome arm 2BS near the locus Xfbb121-2B, co-segregated with the gene Ppd-B1 known to be involved in photoperiod response. This chromosome region explained a large part of the variation (23.4-44.4% depending on the years or the traits). Another region located on chromosome arm 7BS between the loci Xfbb324-7B and Xfbb53-7B also had a strong effect (7.3-15.3%). This region may correspond to a QTL for earliness per se. 相似文献
15.
《Genomics》2022,114(3):110358
To date, very little research on drought tolerance has been conducted at the seedling stage in winter wheat. In this study, two types of traits, namely tolerance and recovery traits, associated with drought tolerance were scored in biparental mapping population (BPP) and association mapping population (A-set). The results of this study revealed no or weak significant correlation between the two types of traits. Based on GWAS and QTL mapping analyses, all QTLs associated with recovery traits were completely different from those associated with tolerance traits except one QTL in each population that was found to be associated with one tolerance trait and one recovery trait. The analysis of SNP and gene networks confirmed the results of combined GWAS and QTL mapping. One SNP marker located on the 2B chromosome (S2B_26494801) was found to be associated with recovery traits in both populations. The results of this study provided new information on understanding and improving drought tolerance in winter wheat. 相似文献
16.
Clerkx EJ El-Lithy ME Vierling E Ruys GJ Blankestijn-De Vries H Groot SP Vreugdenhil D Koornneef M 《Plant physiology》2004,135(1):432-443
Quantitative trait loci (QTL) mapping was used to identify loci controlling various aspects of seed longevity during storage and germination. Similar locations for QTLs controlling different traits might be an indication for a common genetic control of such traits. For this analysis we used a new recombinant inbred line population derived from a cross between the accessions Landsberg erecta (Ler) and Shakdara (Sha). A set of 114 F9 recombinant inbred lines was genotyped with 65 polymerase chain reaction-based markers and the phenotypic marker erecta. The traits analyzed were dormancy, speed of germination, seed sugar content, seed germination after a controlled deterioration test, hydrogen peroxide (H2O2) treatment, and on abscisic acid. Furthermore, the effects of heat stress, salt (NaCl) stress, osmotic (mannitol) stress, and natural aging were analyzed. For all traits one or more QTLs were identified, with some QTLs for different traits colocating. The relevance of colocation for mechanisms underlying the various traits is discussed. 相似文献
17.
Na‐Young Ro On‐Sook Hur Joung‐Ho Lee Jin‐Kyung Kwon Byoung‐Cheorl Kang 《Plant biotechnology journal》2018,16(9):1546-1558
Capsaicinoids are unique compounds produced only in peppers (Capsicum spp.). Several studies using classical quantitative trait loci (QTLs) mapping and genomewide association studies (GWAS) have identified QTLs controlling capsaicinoid content in peppers; however, neither the QTLs common to each population nor the candidate genes underlying them have been identified due to the limitations of each approach used. Here, we performed QTL mapping and GWAS for capsaicinoid content in peppers using two recombinant inbred line (RIL) populations and one GWAS population. Whole‐genome resequencing and genotyping by sequencing (GBS) were used to construct high‐density single nucleotide polymorphism (SNP) maps. Five QTL regions on chromosomes 1, 2, 3, 4 and 10 were commonly identified in both RIL populations over multiple locations and years. Furthermore, a total of 109 610 SNPs derived from two GBS libraries were used to analyse the GWAS population consisting of 208 C. annuum‐clade accessions. A total of 69 QTL regions were identified from the GWAS, 10 of which were co‐located with the QTLs identified from the two biparental populations. Within these regions, we were able to identify five candidate genes known to be involved in capsaicinoid biosynthesis. Our results demonstrate that QTL mapping and GBS‐GWAS represent a powerful combined approach for the identification of loci controlling complex traits. 相似文献
18.
QTL analysis for grain protein content using SSR markers and validation studies using NILs in bread wheat 总被引:11,自引:0,他引:11
Prasad M Kumar N Kulwal PL Röder MS Balyan HS Dhaliwal HS Gupta PK 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2003,106(4):659-667
QTL interval mapping for grain protein content (GPC) in bread wheat was conducted for the first time, using a framework map based on a mapping population, which was available in the form of 100 recombinant inbred lines (RILs). The data on GPC for QTL mapping was recorded by growing the RILs in five different environments representing three wheat growing locations from Northern India; one of these locations was repeated for 3 years. Distribution of GPC values followed normal distributions in all the environments, which could be explained by significant g x e interactions observed through analyses of variances, which also gave significant effects due to genotypes and environments. Thirteen (13) QTLs were identified in individual environments following three methods (single-marker analysis or SMA, simple interval mapping or SIM and composite interval mapping or CIM) and using LOD scores that ranged from 2.5 to 6.5. Threshold LOD scores (ranging from 3.05 to 3.57), worked out and used in each case, however, detected only seven of the above 13 QTLs. Only four (QGpc.ccsu-2B.1; QGpc.ccsu-2D.1; QGpc.ccsu-3D.1 and QGpc.ccsu-7A.1) of these QTLs were identified either in more than one location or following one more method other than CIM; another QTL (QGpc.ccsu-3D.2), which was identified using means for all the environments, was also considered to be important. These five QTLs have been recommended for marker-assisted selection (MAS). The QTLs identified as above were also validated using ten NILs derived from three crosses. Five of the ten NILs possessed 38 introgressed segments from 16 chromosomes and carried 42 of the 173 markers that were mapped. All the seven QTLs were associated with one or more of the markers carried by the above introgressed segments, thus validating the corresponding markers. More markers associated with many more QTLs to be identified should become available in the future by effective MAS for GPC improvement. 相似文献
19.
Location of genes involved in ear compactness in wheat (Triticum aestivum) by means of molecular markers 总被引:4,自引:0,他引:4
Sourdille P. Tixier M.H. Charmet G. Gay G. Cadalen T. Bernard S. Bernard M. 《Molecular breeding : new strategies in plant improvement》2000,6(3):247-255
Quantitative trait loci (QTLs) for three traits related to ear morphology (spike length, number of spikelets, and compactness as the ratio between number of spikelets and spike length) in wheat (Triticum aestivum L.) were mapped in a doubled-haploid (DH) population derived from the cross between the cultivars Courtot and Chinese Spring. A molecular marker linkage map of this cross that had previously been constructed based on 187 DH lines and 380 markers was used for QTL mapping. The genome was well covered (85%) except chromosomes 1D and 4D and a set of anchor loci regularly spaced (one marker each 15.5 cM) were chosen for marker regression analysis. The presence of a QTL was declared at a significance threshold = 0.001. The population was grown in one location under field conditions during three years (1994, 1995 and 1998). For each trait, 4 to 6 QTLs were identified with individual effects ranging between 6.9% and 21.8% of total phenotypic variation. Several QTLs were detected that affected more than one trait. Of the QTLs 50% were detected in more than one year and two of them (number of spikelets on chromosome 2B, and compactness on chromosome 2D) emerged from the data from the three years. Only one QTL co-segregated with the gene Q known to be involved in ear morphology, namely the speltoid phenotype. However, this chromosome region explained only a minor part of the variation (7.5–11%). Other regions had a stronger effect, especially two previously unidentified regions located on chromosomes 1A and 2B. The region on the long arm of chromosome 1A was close to the locus XksuG34-1A and explained 12% of variation in spike length and 10% for compactness. On chromosome 2B, the QTL was detected for the three traits near the locus Xfbb121-2B. This QTL explained 9% to 22% of variation for the traits and was located in the same region as the gene involved in photoperiod response (Ppd2). Other regions were located at homoeologous positions on chromosomes 2A and 2D. 相似文献
20.
Genomic regions affecting seed shattering and seed dormancy in rice 总被引:43,自引:0,他引:43
H.-W. Cai Hiroko Morishima 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(6):840-846
Non-shattering of the seeds and reduced seed dormancy were selected consciously and unconsciously during the domestication
of rice, as in other cereals. Both traits are quantitative and their genetic bases are not fully elucidated, though several
genes with relatively large effects have been identified. In the present study, we attempted to detect genomic regions associated
with shattering and dormancy using 125 recombinant inbred lines obtained from a cross between cultivated and wild rice strains.
A total of 147 markers were mapped on 12 rice chromosomes, and QTL analysis was performed by simple interval mapping and composite
interval mapping. For seed shattering, two methods revealed the same four QTLs. On the other hand, for seed dormancy a number
of QTLs were estimated by the two methods. Based on the results obtained with the intact and de-hulled seeds, QTLs affecting
hull-imposed dormancy and kernel dormancy, respectively, were estimated. Some QTLs detected by simple interval mapping were
not significant by composite interval mapping, which reduces the effects of residual variation due to the genetic background.
Several chromosomal regions where shattering QTLs and dormancy QTLs are linked with each other were found. This redundancy
of QTL associations was explained by ”multifactorial linkages” followed by natural selection favoring these two co-adapted
traits.
Received: 23 November 1998 / Accepted: 27 August 1999 相似文献