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1.
材料和方法:在同一病人新鲜组织中选4枚为0.7×0.6×0.6cm大小的淋巴结备用,先后作了5例。将1枚淋巴结速冻做冰冻切片4μm,另将3枚一分为二,厚度为0.2cm。把6块标本分别放入以下固定液中:①10%福尔马林。②95%酒精。③AF液(福尔马林... 相似文献
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蝴蝶兰根段的组织培养 总被引:36,自引:2,他引:36
1 植物名称 蝴蝶兰(PhalaenopsisMellerGold“NFS”)。2 材料类别 根段。3 培养条件 (1)愈伤组织的诱导及分化培养基:B5+NAA1.5mg·L-1(单位下同)+KT0.2+CM150ml·L-1+3%蔗糖;(2)原球茎增殖培养基:B5+GA0.05+CH120+3%蔗糖;(3)小苗生长培养基:1/2MS+20%香蕉泥+2%蔗糖;(4)诱导生根培养基:1/2MS+IBA0.3+2%蔗糖。上述培养基均加0.2%活性炭,0.58%琼脂粉,pH为5.5;培养基在121℃高… 相似文献
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紫菀花序芽培养及植株再生 总被引:1,自引:0,他引:1
1植物名称紫菀(Astertataricus)。2材料类别花序芽。3培养条件愈伤组织、不定芽诱导及增殖培养基:(1)MS+6-BA0.5~2.0mg·L-1(单位下同);生根培养基:(2)MS+NAA0.5~2.0+6-BA0.2,(3)1/2MS(大量元素减半)+NAA0.5~2.0+6-BA0.2。上述培养基均加0.8%琼脂和3%蔗糖,高温高压灭菌前pH值调至5.8。培养温度为(2312)℃,每天光照12h,光照度为1500~2000lx4生长与分化情况4.1愈伤组织及不定芽的诱导剪取长约0… 相似文献
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《中国组织化学与细胞化学杂志》2016,(3)
目的探讨碳蜡(聚乙二醇)包埋技术、冰冻切片及饿酸染色在脂肪染色中的应用,综合比较几种方法的优缺点,以便在病理工作及科研工作中能找到更适合的脂肪染色方法。方法取新鲜脂肪组织及肝组织,每份标本各取3块组织,分为A、B、C三组:A组和B组标本分别进行碳蜡包埋切片和常规冰冻切片,油红O法染色;C组以饿酸浸染组织块后进行石蜡包埋切片及眦复染。结果 A组切片脂肪滴呈红色,细胞核呈蓝色;B组切片脂肪滴呈红色,细胞核呈蓝色;C组切片脂肪滴呈黑色。结论饿酸染色和碳蜡包埋技术在脂肪染色中,具有冰冻切片和石蜡切片的优点,弥补了常规冰冻切片脂肪染色的缺点和局限性,在病理检验及科研中具有一定应用价值。 相似文献
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藏红花愈伤组织诱导及其细胞培养的研究 总被引:4,自引:0,他引:4
藏红花幼叶愈伤组织的诱导频率在MS,B5和White三种培养基上均高达98%;幼叶和芽的诱导率差异不大,高达99%;不同时期的叶片差异较大,以幼叶诱导为佳;球茎诱导率为近80%;激素配比以2.4-D2.0mg/L,BAP0.1~0.5ml/L为宜。在继代培养阶段,MS比B5和White更适合细胞的快速生长繁殖;并且以叶片的愈伤组织生长较快,芽次之,球茎最慢,适合于细胞生长的激素配比为NAA2.0~3.0mg/L,BAP0.5~1.0mg/L为宜。 相似文献
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宫粉郁金的组织培养和快速繁殖 总被引:7,自引:1,他引:7
1植物名称宫粉郁金(Curcuma kwangsiensis)。2材料类别 块茎萌动芽。3培养条件 萌动芽生长培养基:(1)MS+6-BA1mg·L-1(单位下同)+NAA0.2。不定芽增殖与愈伤组织诱导培养基:(2)MS+6-BA10+KT5;(3)MS+6.BA10;(4)MS+6-BA5+KT2.5;(5)MS+6-BA5;(6)MS+6-BA2+KT1。生根培养基:(7)MS+NAA0.5;(8)MS+6-BA0.5+NAA0.5;(9)MS。以上培养基均加0.7%琼脂,3%蔗糖,pH5… 相似文献
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沙葱的组织培养和植株再生 总被引:7,自引:0,他引:7
1植物名称沙葱(Alliummongolicum),又名蒙古韭。种子来源于中国科学院沙漠研究所宁夏沙坡头沙漠试验站。2材料类别幼苗的叶片。3培养条件(1)愈伤组织诱导培养基:MS+2,4-D2.0mg·L-1(单位下同)+KT0.2+CH(水解酪蛋白)500+蔗糖3%;(2)分化培养基同(1),但2,4-D为0.5;(3)生根培养基:1/2MS+1BA1.0+蔗糖3%。上述培养基均加0.7%琼脂粉固化,pH调至5.8~6.2。培养温度(25±2)℃。愈伤组织诱导阶段为暗培养,分化阶段每日光照12… 相似文献
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菜心下胚轴原生质体培养和植株再生 总被引:6,自引:0,他引:6
以萌发3—4 天(长约4 cm )的菜心(Brassica campestris var.parachinesis)无菌苗苍白下胚轴为材料,酶解分离原生质体。经纯化的原生质体,在含0.5 m g/LZT、0.5 m g/L2,4-D、1.0 m g/LNAA 和0.4 m ol/L葡萄糖的K8p 培养基中,进行微滴培养。在起始培养14—18小时,原生质体再生新的细胞壁。36 小时再生细胞开始第一次分裂。第三天分裂细胞频率可达35% 。培养第8—9 天,可见含8—16个细胞的小细胞团,植板率为15% —18% 。3 周后将发育成直径为2 m m 的白色小愈伤组织,转到含0.3 m g/L 2,4-D并用gelrite半固化的培养基上,增殖成4—5 m m 直径的愈伤组织。在MS+ 3.2(或1.6) m g/L BA+ 1.6(或0.8) m g/LZT+ 0.01 m g/L NAA+ 0.1 m g/LGA3 和0.2% 蔗糖的分化培养基上,获得芽的分化。切下约2 cm 长的芽苗,转移到含0.2 m g/LIAA 和2% 蔗糖的培养基上,生根形成完整植株 相似文献
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本实验应用血脂检测及肠道菌群检查法,探讨了对人工高血脂症小鼠模型灌喂厌氧-1号活菌制剂后,小鼠血脂的变化。结果表明:每天给予模型小鼠厌氧-1号菌液(1亿活菌/毫升)0.5毫升,第7天血指即明显下降,与空白对照(自然恢复)组比有显著性差别,血清胆固醇(P<0.01),降低率为45.1%;高密度脂蛋白胆固醇(P<0.05),降低率为49.1%;甘清三酯(P<0.05),降低率为19.2%。到第14天时血脂均已恢复至正常水平,而自然恢复组小鼠血清胆固醇水平仍比正常对照组略高。 相似文献
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香石竹的叶片培养及植株再生 总被引:14,自引:0,他引:14
1植物名称香石竹(Dianthuscaryophy-llus),别名康乃馨。2材料类别无菌苗叶片。3培养条件以MS为基本培养基。分化培养基附加:(1)6-BA1.0mp·L-1(单位下同)+NAA0.3;(2)6-BA1.0+NAA0.1;(3)6-BA1.0+NAA0.05。增殖培养基附加6-BA0.5+NAA0.1。分化和增殖培养基均加蔗糖3%、琼脂0.7%,pH5.8。生根培养基为1/2MS附加NAA0.1,蔗糖2%,琼脂0.6%,pH5.8。培养温度为(25±1)℃,光照12h·d-1,… 相似文献
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BACKGROUND:
Hemophilia A (HA), being an X-linked recessive disorder, females are rarely affected, although they can be carriers.AIMS:
To study the mutation in F8 gene in an extended family with a homozygous female HA.MATERIALS AND METHODS:
All the seven affected members (six males and one female) were initially screened by Conformation Sensitive Gel Electrophoresis (CSGE) and direct DNA sequencing.RESULTS:
A homozygous missense mutation c.1315G>A (p.Gly420Ser) was identified in exon 9 of F8 gene in homozygous state in the affected female born of 1° consanguinous marriage and in all the affected male members of the family. Her factor VIII levels was found to be 5.5%, vWF:Ag 120%.CONCLUSION:
In India, as consanguineous marriages are very common in certain communities (up to 30%), the likelihood of encountering female hemophilia is higher, although this is the first case of HA out of 1600 hemophilia families registered in our Comprehensive Haemophilia Care Center. Genetic diagnosis in such cases is not necessary as all the male children will be affected and daughters obligatory carriers. 相似文献12.
N.A.Abdel Salam Zeinab F. Mahmoud Jürgen Ziesche Ferdinand Bohlmann 《Phytochemistry》1982,21(11):2746-2747
The aerial parts of Urospermum picroides afforded, in addition to urospermal A a p-hydroxylphenyl acetate of a glucoside of urospermal A. 相似文献
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Replacement of two to four guanines by adenines in the human telomere DNA repeat dG3(TTAG3)3 did not hinder the formation of quadruplexes if the substitutions took place in the terminal tetrad bridged by the diagonal loop of the intramolecular antiparallel three‐tetrad scaffold, as proved by CD and PAGE in both Na+ and K+ solutions. Thermodynamic data showed that, in Na+ solution, the dG3(TTAG3)3 quadruplex was destabilized, the least by the two G:A:G:A tetrads, the most by the G:G:A:A tetrad in which the adenosines replaced syn‐guanosines. In physiological K+ solution, the highest destabilization was caused by the 4A tetrad. In K+, only the unmodified dG3(TTAG3)3 quadruplex rearranged into a K+‐dependent quadruplex form, none of the multiple adenine‐modified structures did so. This may imply biological consequences for nonrepaired A‐for‐G mutations. © 2010 Wiley Periodicals, Inc. Biopolymers 93: 880–886, 2010. 相似文献
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P. Kugler 《Histochemistry and cell biology》1982,74(2):229-245
Summary Quantitative histochemical measurements of aminopeptidase A (APA; E.C.3.4.11.7) were done kinetically in the kidney glomeruli of rat and mouse with an instrumental setup consisting of a microdensitometer and a computer-supported morphometric system. The histochemical demonstration of APA was carried out using the simultaneous azo coupling technique (purest-grade Fast Blue B as coupling agent and -l-glutamic acid-4-methoxy-2-naphthylamide as substrate). The methodological studies show that APA activity is calcium-ion-dependent and increases linearly with the thickness of the tissue section (3–12 m) and that the time-course of APA activity as determined by linear regression is linear only for the first 1 to 2 min of the reaction. — Kinetic measurements indicate a 40% decrease in APA activities when -l-glutamic acid-4-methoxy-2-naphthylamide (-l-Glu-MNA) is replaced by -l-aspartic acid-4-methoxy-2-naphthylamide. When -l-Glu-MNA is replaced with l-alanine-4-methoxy-2-naphthylamide, which is a substrate of aminopeptidase M (APM) only very low reaction rates are measurable (about 1.4% of those with -l-Glu-MNA). 100 and 130 mM NaCl in the incubation medium increase APA activities by approximately 16%–17%. — To clarify the functional importance of APA in the kidney, their activities were measured under the influence of angiotensins. The glomerulus was selected as the measuring site, for besides APA it contains no APM or other peptidases that could degrade angiotensins (the glomerular dipeptidyl peptidase IV is not inhibited by angiotensin II). Using the Lineweaver-Burk plot, we determined a K
m of 0.16 mM for the APA in rat glomeruli and 0.14 mM in mouse glomeruli. The V
max in mouse glomeruli is 1.6 times higher than in rat glomeruli. Ang iotensin I, II and III competitively inhibit APA in the rat and mouse glomeruli. — With quantitative histochemical techniques it was possible to show that APA is equivalent to angiotensinase A (splitting off the N-terminal aspartic acid from angiotensin I and II).Supported by the Deutsche Forschungsgemeinschaft (SFB 105) 相似文献
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有或没有基细胞是毛鞘藻属(Bulbochaets)与枝鞘藻属(Oedocladium)的区别之一。这里叙述了Mrozinska在其专著(1985)中,将Oedocladium indicum Kama附图(即模式图)上的一个基细胞错误地移置到Oe.PrescottiiIslam上去的情况。 相似文献