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1.
Gerhard Thiel 《European biophysics journal : EBJ》1995,24(2):85-92
TheCl– and K+ currents underlying the action potential (AP) in the giant alga Chara were directly recorded with the action potential clamp method. An electrically triggered action potential was recorded and repetitively replayed as command voltage to the same cell under voltage clamp. The resulting clamp current was close to zero. Only the initial rectangular current used for stimulation was approximately reproduced by the clamp circuit. Inhibition of Cl– channels with niflumic acid or ethacrynic acid and of K+ channels with Ba2+ evoked characteristic compensation currents because the amplifier had to add the selectively inhibited currents. Integration of the compensation currents revealed a mean flux through Cl– and K+ channels of 3.3 10–6 and 2.1 10–6 mole M–2 AP–1 respectively. The dynamics of CI– and K+ channel activation/inactivation were obtained by converting the relevant clamp currents to ionic permeabilities using the Goldman-Hodgkin-Katz current equation. During the AP the Cl– permeability reaches a peak 370 ms, on average, after termination of the stimulating pulse. The following inactivation proceeds 3.6 times slower than the activation. The increase in K+ permeability lags behind the rise in Cl– permeability, reaching a peak approximately 2 s after the latter. 相似文献
2.
W. -M. Weber K. M. Liebold F. W. Reifarth W. Clauss 《The Journal of membrane biology》1995,148(3):263-275
Defolliculated oocytes of Xenopus laevis responded to removal of external divalent cations with large depolarizations and, when voltage clamped, with huge currents. Single channel analysis revealed a Cl– channel with a slope conductance of about 90 pS at positive membrane potentials with at least four substates. Single channel amplitudes and mean channel currents had a reversal potential of approximately –15 mV as predicted by the Nernst equation for a channel perfectly selective for Cl–. Readdition of Ca2+ immediately inactivated the channel and restored the former membrane potential or clamp current. The inward currents were mediated by a Ca2+ inactivated Cl– channel (CaIC). The inhibitory potency of Ca2+ was a function of the external Ca2+ concentration with a half maximal blocker concentration of about 20 m.These channels were inhibited by the Cl– channel blockers flufenamic acid, niflumic acid and diphenylamine-2-carboxylate (DPC). In contrast, 4,4-acetamido-4-isothiocyanatostilbene-2,2-disulfonicacid (SITS), another Cl– channel blocker, led to activation of this Cl– channel. Like other Cl– channels, the CaIC was activated by cytosolic cAMP. Extracellular ATP inhibited the channel while ADP was without any effect. Injection of phorbol 12-myristate 13-acetate (PMA), a protein kinase C activating phorbol ester, stimulated the Cl– current. Cytochalasin D, an actin filament disrupting compound, reversibly decreased the clamp current demonstrating an influence of the cytoskeleton.The results indicate that removal of divalent cations activates Cl– channels in Xenopus oocytes which share several features with Cl– channels of the CLC family. The former so-called leak current of oocytes under divalent cation-free conditions is nothing else than an activation of Cl– channels.The microelectrode measurements are part of the PhD thesis of K. Liebold; the patch clamp contributions are part of the PhD thesis of F.W. Reifarth. This study was supported by the Deutsche Forschungsgemeinschaft (We1858/2-l) and by Sonderforschungsbereich 249. 相似文献
3.
Barrière H Rubera I Belfodil R Tauc M Tonnerieux N Poujeol C Barhanin J Poujeol P 《The Journal of membrane biology》2003,193(3):153-170
Volume-sensitive chloride and potassium currents were studied, using the whole-cell clamp technique, in cultured wild-type mouse proximal convoluted tubule (PCT) epithelial cells and compared with those measured in PCT cells from null mutant kcne1 –/– mice. In wild-type PCT cells in primary culture, a Cl– conductance activated by cell swelling was identified. The initial current exhibited an outwardly rectifying current-voltage (I-V) relationship, whereas steady-state current showed decay at depolarized membrane potentials. The ion selectivity was I– > Br– > Cl– >> gluconate. This conductance was sensitive to 1 mM 4,4-Diisothiocyanostilbene-2,2-disulfonic acid (DIDS), 0.1 mM 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB) and 1 mM diphenylamine-2-carboxylate (DPC). Osmotic stress also activated K+ currents. These currents are time-independent, activated at depolarized potentials, and inhibited by 0.5 mM quinidine, 5 mM barium, and 10 µM clofilium but are insensitive to 1 mM tetraethylammonium (TEA), 10 nM charybdotoxin (CTX), and 10 µM 293B. In contrast, the null mutation of kcne1 completely impaired volume-sensitive chloride and potassium currents in PCT. The transitory transfection of kcne1 restores both Cl– and K+ swelling-activated currents, confirming the implication of KCNE1 protein in the cell-volume regulation in PCT cells in primary cultures. 相似文献
4.
The cystic fibrosis transmembrane conductance regulator (CFTR) is a chloride (Cl−) channel known to influence the function of other channels, including connexin channels. To further study potential functional interactions between CFTR and gap junction channels, we have co-expressed CFTR and connexin45 (Cx45) in Xenopus oocytes and monitored junctional conductance and voltage sensitivity by dual voltage clamp electrophysiology. In single oocytes expressing CFTR, an increase in cAMP caused by forskolin application induced a Cl− current and increased membrane conductance; application of diphenylamine carboxylic acid (CFTR blocker) readily blocked the Cl− current. With co-expression of CFTR and Cx45, application of forskolin to paired oocytes induced a typical outward current and increased junctional conductance (Gj). In addition, the presence of CFTR reduced the transjunctional voltage sensitivity of Cx45 channels without affecting the kinetics of junctional current inactivation. The drop in voltage sensitivity was further enhanced by forskolin application. The data indicate that CFTR influences cell-to-cell coupling mediated by Cx45 channels. 相似文献
5.
An anion channel that only allows outward current flow (anion influx) has been identified in protoplasts derived from wheat (Triticum aestivum L., Triticum turgidum L.) roots. The anion outward rectifier (anion OR) measured by patch-clamp of whole cells activated very quickly, usually reaching a steady-state level in less than 100 ms and was easily distinguished from the cation outward rectifier (cation OR) which activated more slowly during membrane depolarisation. The anion OR is permeable to NO
3
–
and Cl–, moderately permeable to I–, and relatively impermeable to H2PO4/– and ClO4/–. An anomalous mole-fraction effect between ClO4/
– and Cl– was observed on the outward current, indicating that the channel is a multi-ion pore. The anion OR is gated by both voltage and external anion concentration such that it activates near to the equilibrium potential for the permeant anion. It activated at more negative membrane potentials when NO
3
–
was substituted for Cl– in the external medium, indicating that the channel may function to allow NO
3
–
influx under luxuriant external NO
3
–
concentrations. For most experiments, K+ and Cl– were the main cation and anion in solution, and under these conditions it appeared likely that the anion OR functioned in membrane-potential regulation by facilitating a Cl– influx at membrane potentials more positive than the chloride reversal potential (ECl). If ECl was more negative than the K+ reversal potential (EK) then the anion OR dominated but both the anion and cation ORs occurred together when the membrane potential difference (Vm) was positive of both ECl and EK. The cation OR was inhibited by increasing external Cl– concentrations, but the anion OR was not affected by external K+ or Na+ concentration. The anion-transport inhibitors, zinc and phenylglyoxal were ineffective in blocking the anion OR. 4,4-Di-isothiocyanostilbene-2, 2-disulfonic acid (DIDS) irreversibly blocked about 34% of the current when applied extracellularly at a concentration of 25 M, and about 69% at a concentration of 200 M. However, DIDS (200 M) also occasionally acted as an irreversible blocker of the cation OR. Perchlorate blocked irreversibly 75% of the current at an external concentration of 10 mM and did not block the cation OR. Whole-cell currents also indicated that the anion OR was insensitive to external pH (pH=5–7) and calcium concentration ([Ca2+]=0.1–10 mM). Increasing intracellular calcium concentration significantly increased the occurrence of the fast outward current in whole cells (P < 0.005, X2 test). With approximately 10 nM calcium inside the cell the anion outward current was observed in 64% (n = 45) of cells and with 50 nM calcium inside the cell the anion current was observed in 88% (n = 69) of cells. Single-anion OR channels observed in outside-out patches had a conductance in 300 mM KCl (external) of about 4 pS. When voltage pulses were applied to outside-out patches the average currents were similar to those observed in whole cells. The significance of the anion OR as a likely route for Cl uptake in high salinities is discussed.Abbreviations Bath
solution bathing the extracellular face of the membrane
- DIDS
(4,4-diisothiocyanostilbene-2,2-disulfonic acid)
- Ex
reversal potential for ion x
- OR
outward rectifier
-
Pip
solution inside the pipette
- TEACl
(tetraethyl-ammonium chloride)
- Vm
membrane potential difference
We thank the Australian Research Council for financial support, G.P. Findlay and A. Garrill for helpful discussions, and K. Morris and D. Mackenzie for expert technical assistance. M.S. was supported by an Australian Postgraduate Research Award. 相似文献
6.
Ugarte G Delgado R O'Day PM Farjah F Cid LP Vergara C Bacigalupo J 《The Journal of membrane biology》2005,207(3):151-160
We report that Drosophila retinal photoreceptors express inwardly rectifying chloride channels that seem to be orthologous to mammalian ClC-2 inward
rectifier channels. We measured inwardly rectifying Cl− currents in photoreceptor plasma membranes: Hyperpolarization under whole-cell tight-seal voltage clamp induced inward Cl− currents; and hyperpolarization of voltage-clamped inside-out patches excised from plasma membrane induced Cl− currents that have a unitary channel conductance of ∼3.7 pS. The channel was inhibited by 1 mM Zn2+ and by 1 mM 9-anthracene, but was insensitive to DIDS. Its anion permeability sequence is Cl− = SCN−> Br−>> I−, characteristic of ClC-2 channels. Exogenous polyunsaturated fatty acid, linolenic acid, enhanced or activated the inward
rectifier Cl− currents in both whole-cell and excised patch-clamp recordings. Using RT-PCR, we found expression in Drosophila retina of a ClC-2 gene orthologous to mammalian ClC-2 channels. Antibodies to rat ClC-2 channels labeled Drosophila photoreceptor plasma membranes and synaptic regions. Our results provide evidence that the inward rectification in Drosophila retinal photoreceptors is mediated by ClC-2-like channels in the non-transducing (extra-rhabdomeral) plasma membrane, and
that this inward rectification can be modulated by polyunsaturated fatty acid.
G. Ugarte and R. Delgado contributed equally to this work. 相似文献
7.
The whole-cell patch-clamp technique has been used to study membrane currents in cultured rabbit medullary thick ascending limb (MTAL) epithelial cells. A Ca2+-activated K+ current was characterized by its voltage-dependent and Ca2+-dependent properties. When the extracellular K+ ion concentration was increased from 2 to 140 mm, the rereversal potential (Ek) was shifted from –85 to 0 mV with a slope of 46 mV per e-fold change. The Ca2+-activated K+ current is blocked by charybdotoxin (CTX) in a manner similar to the apical membrane Ca2+-activated K+ channel studied with the single channel patch-clamp technique. The results suggest that the Ca2+-activated K+ current is the predominant, large conductance and Ca2+-dependent K+ pathway in the cultured MTAL cell apical membrane. The biophysical properties and physiological regulation of a Cl– current were also investigated. This current was activated by stimulation of intracellular cAMP using forskolin and isobutyl-1-methylxanthine (IBMX). The current-voltage (I–V) relationship of the Cl– current showed an outward-rectifying pattern in symmetrical Cl– solution. The Cl– selectivity of the whole-cell current was confirmed by tail current analysis in different Cl– concentration bath solutions. Several Cl– channel blockers were found to be effective in blocking the outward-rectifying Cl– current in MTAL cells. The cAMP-dependent Cl– transport in MTAL cells was further confirmed by measuring changes in the intensity of Cl– sensitive dye using fluorescence microscopy. These results suggest that the Cl– channel in the apical or basolateral membrane of MTAL cells may be regulated by cAMP-dependent protein-kinase-induced phosphorylation.This study was supported by the National Institutes of Health grants GM46834 to L.L. and DK32753 to W.B.G., and by a Grant-in-Aid from the American Heart Association of Ohio to L.L. 相似文献
8.
Cl– conductance in cultured embryonic chick cardiac myocytes was characterized using whole-cell patch clamp techniques. Following elimination of cation currents in Na+and K+-free internal and external solutions, the basal whole-cell current was predominantly a Cl– current. Cl–-sensitive current (I
Cl) was defined as the difference between the whole-cell currents recorded in normal and low [Cl–]
o
when measured in the same cell. The whole-cell current in the absence or presence of 10 m cAMP was time independent, displayed outward rectification with the pipette [Cl–] < 40 mm, and was not saturated with a physiological Cl– gradient. The Cl– current was also activated by 1 m forskolin and inhibited by 0.3 mm anthracene-9-carboxylic acid (9-AC). Forskolin was less effective than cAMP (internal dialysis) in activating the Cl– current. The cAMP- or forskolin-activated and basal Cl– current were reasonably fit by the Goldman-Hodgkin-Katz equation. The calculated P
Cl in the presence of cAMP was increased by fiveto sixfold over the basal level. In the presence of 5 mm EGTA to decrease free [Ca2+]
i
, the whole-cell current could not be stimulated by cAMP, forskolin or IBMX (0.1 mm). These data suggest that cultured chick cardiac myocytes have a low basal Cl– conductance, which, as in some mammalian cardiac ventricular myocytes, can be activated by cAMP. However, this study shows that the activation process requires physiological free [Ca2+]
i
.This study was supported by grants from the National Institutes of Health (HL-17670, HL-27105 and HL-07107) for M.L. and by Institutional funds of the University of Arkansas for Medical Sciences for S.L.We thank Meei-Yueh Liu, Kathleen Mitchell, and Shirley Revels for their technical assistance. 相似文献
9.
F. Trischitta M. G. Denaro C. Faggio F. Fucile T. Schettino 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1994,164(4):286-291
The sensitivity to external pH of Cl- absorption was studied in isolated stripped intestinal mucosa of the eel, Anguilla anguilla, mounted in Ussing chambers. Short-circuit current, transepithelial potential difference and conductance were measured in bathing solutions containing various combinations of HCO3
--concentration (0–25 mmol·l-1), partial pressure of CO2 (0–76 mm Hg) and pH (6.9–7.9). A linear relationship was found between pH and short-circuit current in the range of pH studied both in HCO3
-/CO2 Ringer and in Hepes Ringer. The pH effect was almost completely reversible. It was not affected by the presence of mucosal Ba2+ (10-3 mol·l-1) but it was inhibited by the presence of luminal (10-5 mol·l-1) or serosal (10-4 mol·l-1) bumetanide. The results obtained suggest that the Cl- absorption in the European eel intestine is pH sensitive. The data do not indicate whether the pH affects directly the Na+–K+–Cl- cotransport and/or the basolateral Cl- conductance or other mechanisms indirectly linked to Cl- absorption.Abbreviations g
t
transepithelial conductance
- Hepes
N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid
-
I
sc
short circuit current
-
R
t
transepithelial resistence
-
V
t
transepithelial potential difference 相似文献
10.
Alteration of anion channel kinetics in wild-type and abi1-1 transgenic Nicotiana benthamiana guard cells by abscisic acid 总被引:3,自引:1,他引:2
Alexander Grabov Jeffrey Leung Jerome Giraudat Michael R. Blatt 《The Plant journal : for cell and molecular biology》1997,12(1):203-213
The influence of the plant water-stress hormone abscisic acid (ABA) on anion channel activity and its interaction with protein kinase and phosphatase antagonists was examined in stomatal guard cells of wild-type Nicotiana benthamiana L. and of transgenic plants expressing the dominant-negative (mutant) Arabidopsis abi1-1 protein phosphatase. Intact guard cells were impaled with double-barrelled micro-electrodes and membrane current was recorded under voltage clamp in the presence of 15 mM CsCI and 15 mM tetraethylammonium chloride (TEA-CI) to eliminate K+ channel currents. Under these conditions, the free-running voltage was situated close to 0 mV (+9 ± 6 mV, n = 18) and the membrane under voltage clamp was dominated by anion channel current (ICl) as indicated from tail current reversal near the expected chloride equilibrium potential, current sensitivity to the anion channel blockers 9-anthracene carboxylic acid and niflumic acid, and by its voltage-dependent kinetics. Pronounced activation of ICl was recorded on stepping from a conditioning voltage of ?250 mV to voltages between ?30 and +50 mV, and the current deactivated with a voltage-dependent halftime at more negative voltages (τ? 0.3 sec at ?150 mV). Challenge with 20 µM ABA increased the steady-state current conductance, gCl, near 0 mV by 1.2- to 2.8-fold and at ?150 mV by 4.5- to sixfold with a time constant of 40 ± 4 sec, and it slowed ICl deactivation as much as fourfold at voltages near ?50 mV, introducing two additional voltage-sensitive kinetic components to these current relaxations. Neither the steady-state and kinetic characteristics of ICl, nor its sensitivity to ABA were influenced by H7 or staurosporine, both broad-range protein kinase antagonists. However, the protein phosphatase 1/2A antagonist calyculin A mimicked the effects of ABA on gCl and current relaxations on its own and exhibited a synergistic interaction with ABA, enhancing ICl sensitivity to ABA three- to four-fold. Quantitatively similar current characteristics were recorded from guard cells of abi1-1 transgenic N. bentamiana, indicating that the abi1-1 protein phosphatase does not influence the ànion current or its response to ABA directly. These results demonstrate that ABA stimulates ICl and modulates its voltage sensitivity. Furthermore, they show that ABA promotes ICl, either by introducing additional long-lived states of the channel or by activating a second anion channel with similar permeation characteristics but with a very long dwell time in the open state. Overall, the data are broadly consistent with the view that ABA action engenders coordinate control of ICl together with guard cell K+ channels to effect solute loss and stomatal closure. 相似文献
11.
12.
F. Zufall Ch. Franke H. Hatt 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1988,163(5):609-620
Summary In conventional two microelectrode experiments, acetylcholine had qualitatively the same effect as GABA and glutamate on membrane potential and input resistance of muscle fibres of the opener and intrinsic stomach muscles of crayfish (Austropotamobius torrentium). In patch-clamp experiments, acetylcholine occasionally elicited single channel openings in cell-attached patches on these muscles. If outside-out patches were excised and the Cl– concentration was high on both sides of the membrane, acetylcholine at concentrations of 1 nM regularly elicited single channel currents. The amplitude of single channel currents depended strongly on the intracellular concentration of Cl–. The reversal potential of the channel, determined after replacing intracellular K+ with Cs+, corresponded to the Nernst potential for Cl–. The voltage dependence and the reversal potential of single channel current amplitudes elicited by ACh, glutamate and GABA were identical. The distribution of life times of openings (>1 ms) elicited by ACh and glutamate could be fitted by a single exponential with a time constant of about 2.5 ms, corresponding to the mean open time. ACh and glutamate applied to the same outside-out patch showed cross-desensitization, and thus ACh and glutamate activate the same channels. An excitatory, cationic ACh-activated channel could not be identified. Permeabilities of the chloride channel were calculated according to the Goldman-Hodgkin-Katz equation at different membrane potentials. Negative single channel current amplitudes (inward currents) could be fitted with a permeability of
2= 3.9×10–14 cm3s–1. For positive currents (outward) the channel had a permeability of
1= 1.4× 10–14 cm3s–1. The permeability of the channel declined from 16×10–14 cm3s–1 to 2.3×10–14 cm3s–1 if the intracellular Cl–-concentration was raised from 6 to 257 mM. The activation elicited by acetylcholine was inhibited by extracellular Ca++. The mean current activated by ACh was reduced by a factor of 50 if the extracellular concentration of Ca++ was raised from 0.1 mM to the physiological concentration of 13.5 mM. 相似文献
13.
The endogenous volume-regulated anion channel (VRAC) from HEK293 cells was pharmacologically characterized using the whole-cell patch-clamp technique. Under isotonic conditions a small (1.3 nS), Ca2+-independent Cl conductance was measured. However, swelling at 75% tonicity activated a VRAC identified as an outward-rectifying anion current (P
l > P
Cl > P
gluconate), which was ATP-dependent and showed inactivation at positive potentials. Activation of this current followed a sigmoid time course, reaching a plateau conductance of 42.6 nS after 12–15 min (t
1/2 = 7 min). The pharmacology of this VRAC was investigated using standard Cl–-channel blockers (NPPB, DIDS, and tamoxifen) as well as a new group (acidic di-aryl ureas) of Cl–-channel blockers (NS1652, NS3623, NS3749, and NS3728). The acidic di-aryl ureas were originally synthezised for inhibition of the human erythrocyte Cl– conductance in vivo. NS3728 was the most potent VRAC blocker in this series (IC
50 = 0.40 µM) and even more potent than tamoxifen (2.2 µM). NS3728 accelerated channel inactivation at positive potentials. These results show that acidic di-aryl ureas constitute a promising starting point for the synthesis of potent inhibitors of VRAC. 相似文献
14.
J. P. Danaceau M. T. Lucero 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1998,183(2):225-235
Isolated olfactory receptor neurons from the squid Lolliguncula brevis respond to betaine, a repellent odorant, with hyperpolarizing receptor potentials. Using perforated-patch techniques, we
determined that the hyperpolarizing conductance was selective for Cl− and could be reversibly blocked by the Cl− channel blockers 4-acetamido-4′-isothio-cyanatistilbene-2,2′disulfonic acid and niflumic acid. Gramicidin-patch recordings
revealed that [Cl−]i in squid olfactory receptor neurons is normally very low compared to vertebrate olfactory receptor neurons, and that activating
a Cl− conductance would hyperpolarize the cell in vivo. The lack of dependence on internal or external K+ or Na+ ruled out the possibility that the Cl− conductance was generated by a cation-dependent cotransporter or pump. Common G-protein-dependent signalling pathways, including
phospholipase C, arachidonic acid, and cyclic nucleotides, do not appear to be involved. Ca2+ imaging experiments showed that betaine did not affect [Ca2+]i, suggesting that the Cl− current is not Ca2+ dependent. Our findings represent the first report of an odorant-activated, hyperpolarizing chloride conductance in olfactory
receptor neurons.
Accepted: 20 March 1998 相似文献
15.
Summary The outer membrane of mechanically prepared protoplasmic droplets fromAcetabularia mediterranea has been investigated by patch-clamp techniques. These membranes are shown to consist of physiologically intact plasmalemma. With the Cl– pump inhibited, microscopic currents through K+-selective channels were studied. These currents compare well with macroscopic K+ currents as previously determined by standard microelectrode techniques and tracer flux measurements. There is about one K+ channel per m2 in the plasmalemma. The current-voltage relationship (I–V curve) of the main open channel (channel A) is sigmoid over a voltage range between about –100 and +100 mV with saturation currents of about ±10 pA. A second species (or different state of channel A) of K+-selective channels (channel B) differs from channel A by smaller saturation currents (about ±7 pA) and a much smaller open probability. The open probability of channel A increases from almost zero at large negative voltages to about 1/2 at large positive voltages. Taking the closed times into account, the mean steady-stateI–V curve of channel A displays outward rectification about the equilibrium voltage for K+ and negative slope conductance at larger negative voltages. The open channelI–V curve of the open channels A and B, the changes of theI–V curve of the open channel A upon variation of the external K+ concentration, as well as the mean steady-stateI–V curves of channel A are described by simple reaction kinetic models, the parameters of which are determined to fit the experimental data. The results are discussed with respect to data from other K+ channels in plants and with respect to regulation of the cytoplasmic K+ concentration inAcetabularia. 相似文献
16.
Patch clamp studies show that there may be as many as seven different channel types in the plasma membrane of protoplasts derived from young leaves of the halophytic angiosperm Zostera muelleri. In whole-cell preparations, both outward and inward rectifying currents that activate in a timeand voltage-dependent manner are observed as the membrane is either depolarized or hyperpolarized. Current voltage plots of the tail currents indicate that both currents are carried by K+. The channels responsible for the outward currents have a unit conductance of approximately 70 pS and are five times more permeable to K+ than to Na+. In outside-out patches we have identified a stretch-activated channel with a conductance of 100 pS and a channel that inwardly rectifies with a conductance of 6 pS. The reversal potentials of these channels indicate a significant permeability to K+. In addition, the plasma membrane contains a much larger K+ channel with a conductance of 300 pS. Single channel recordings also indicate the existence of two Cl– channels, with conductances of 20 and 80 pS with distinct substates. The membrane potential difference of perfused protoplasts showed rapid action potentials of up to 50 mV from the resting level. The frequency of these action potentials increased as the external osmolarity was decreased. The action potentials disappeared with the addition of Gd3+, an effect that is reversible upon washout.We would like to thank K. Morris and D. McKenzie for technical assistance and the Australian Research Council for financial support. 相似文献
17.
Cell swelling has been shown to increase the permeability of the plasma membrane to ions such as K+, Na+, Ca2+ or Cl– in many types of cells. In cardiac cells, swelling has been reported to increase Cl– conductance, but whether cation-selective currents are activated by swelling is not known. Low Cl– or Cl–-free solutions were used to study the presence of such currents. Lowering the osmolarity of the extracellular medium from 299 to 219 mOsm resulted in cell swelling and concurrent activation of a cation-selective whole-cell current. When cell-attached patches were formed on swollen cells, opening of bursting single channel currents were observed in 18% of the patches studied. Ion substitution experiments indicated that the channel discriminated poorly among monovalent cations, and was impermeable to Cl–. The channel was permeable to Ca2+. In symmetrical 140 mM K+, the current-voltage relation was linear with a single channel conductance of 36 ± 3 pS. Depolarization increased channel open probability. Interestingly, depending on the membrane patch studied, application of negative pressure to the pipette caused either an increase or a decrease in the open probability of the channel already activated by swelling. Thus, the sensitivity to tension of the swelling-activated channel was different from those of previously reported stretch-activated channels. These findings suggest that nonselective cation channels exist in rat atrial cells and may be involved in swelling-induced changes in cell function.Dr. Kim is an Established Investigator of the American Heart Association. 相似文献
18.
Adam Bertl 《The Journal of membrane biology》1989,109(1):9-19
Summary The membrane of mechanically prepared vesicles ofChara corallina has been investigated by patch-clamp techniques. This membrane consists of tonoplast as demonstrated by the measurement of ATP-driven currents directed into the vesicles as well as by the ATP-dependent accumulation of neutral red. Addition of 1mm ATP to the bath medium induced a membrane current of about 3.2 mA·m–2 creating a voltage across the tonoplast of about –7 mV (cytoplasmic side negative). On excised tonoplast patches, currents through single K+-selective channels have been investigated under various ionic conditions. The open-channel currents saturate at large voltage displacements from the equilibrium voltage for K+ with limiting currents of about +15 and –30 pA, respectively, as measured in symmetric 250mm KCl solutions. The channel is virtually impermeable to Na+ and Cl–. However, addition of Na+ decreases the K+ currents. TheI–V relationships of the open channel as measured at various K+ concentrations with or without Na+ added are described by a 6-state model, the 12 parameters of which are determined to fit the experimental data. 相似文献
19.
Ordaz B Vaca L Franco R Pasantes-Morales H 《American journal of physiology. Cell physiology》2004,286(6):C1399-C1409
Volume changes and whole cell ionic currents activated by gradual osmolarity reductions (GOR) of 1.8 mosM/min were characterized in C6 glioma cells. Cells swell less in GOR than after sudden osmolarity reductions (SOR), the extent of swelling being partly Ca2+ dependent. In nominally Ca2+-free conditions, GOR activated predominantly whole cell outward currents. Cells depolarized from the initial 79 mV to a steady state of 54 mV reached at 18% osmolarity reduction [hyposmolarity of 18% (H-18%)]. Recordings of Cl and K+ currents showed activation at H-3% of an outwardly rectifying Cl current, with conductance of 1.6 nS, sensitive to niflumic acid and 5-nitro-2-(3-phenylpropylamino)benzoic acid, followed at H-18% by an outwardly rectifying K+ current with conductance of 4.1 nS, inhibited by clofilium but insensitive to the typical K+ channel blockers. With 200 nM Ca2+ in the patch pipette, whole cell currents activated at H-3% and at H-13% cells depolarized from 77 to 63 mV. A K+ current activated at H-1%, showing a rapid increase in conductance, suppressed by charybdotoxin and insensitive to clofilium. These results show the operation of two different K+ channels in response to GOR in the same cell type, activated by Ca2+ and osmolarity and with different osmolarity activation thresholds. Taurine and glutamate efflux, monitored by labeled tracers, showed delayed osmolarity thresholds of H-39 and H-33%, respectively. This observation clearly separates the Cl and amino acid osmosensitive pathways. The delayed amino acid efflux may contribute to counteract swelling at more stringent osmolarity reductions. volume regulation; taurine; hyposmolarity; isovolumetric regulation; regulatory volume decrease 相似文献
20.
Masashi Sawada Mitsuyuki Ichinose George B. Stefano 《Cellular and molecular neurobiology》1994,14(2):175-184
Summary 1. The effects of bath-applied recombinant human interleukin-1 (rhIL-1) and interleukin-2 (rhIL-2) on the calcitonin (CT)-induced outward current recorded from identified neurons (R9–R12) ofAplysia kurodai were investigated with conventional voltage-clamp and pressure ejection techniques.2. Micropressure ejection of CT onto the soma of the neuron induced a slow outward current [I
o(CT); 4–6 nA in amplitude, 30–40 sec in duration] associated with a decrease in input membrane conductance.3.I
o(CT) was increased by hyperpolarization.4. The extrapolated reversal potential was +10 mV. Additionally,I
o(CT) was sensitive to changes in (Na+)o but not to changes in (K+)o, (Ca2+)o, and (Cl–)o.5. Micropressure-ejected forskolin produced a slow outward current similar to that induced by CT.6. Bath-applied rhIL-1 and rhIL-2 (10–40 U/ml) reduced the CT-induced current in identifiedAplysia neurons without affecting the resting membrane conductance or the holding current.7. The inhibitory effects of both cytokines on the current were completely reversible. Heat-inactivated rhIL-1 and rhIL-2 were without effect.8. These results suggest that the immunomodulators, IL-1 and IL-2, can modulate the CT-induced outward current associated with a decrease in Na+ conductance in the nervous system ofAplysia. Therefore, the study suggests that these cytokines may also serve as neuromodulators. 相似文献