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1.
R. D. Reeves A. J. M. Baker T. Becquer G. Echevarria Z. J. G. Miranda 《Plant and Soil》2007,293(1-2):107-119
Major collections of the ultramafic flora of Goiás, central Brazil, were made by Brooks and co-workers in 1988 and 1990. At
the time of reports on this material in 1990–1992 much of it had been identified only tentatively and incompletely, but the
area was clearly interesting for taxonomic and biogeochemical reasons. Further progress has been made but still only two-thirds
of the specimens are identified at the species level. Following a third collection in early 2005, we now have 800 specimens
from this area, with chemical analyses of all the plants and of more than 120 representative soil samples. New species have
been found, e.g., in Paspalum (Poaceae) and Pterolepis (Melastomataceae). There is a need for more taxonomic work in genera such as Cnidoscolus (Euphorbiaceae), Lippia (Verbenaceae), Turnera and Piriqueta (Turneraceae), and Vellozia (Velloziaceae). Ni hyperaccumulation (>1,000 mg/kg in dry plant matter) has now been found in a total of 79 specimens, representing
more than 30 different species. Notable Ni hyperaccumulators include Pfaffia sarcophylla (Amaranthaceae), species of Justicia, Lophostachys and Ruellia (Acanthaceae), Porophyllum (Asteraceae), several species of Lippia (Verbenaceae), Turnera and Piriqueta (Turneraceae), and a possibly new Cnidoscolus (Euphorbiaceae). Ni hyperaccumulation has not been found in plants of the outcrops of Morro Feio or Crominia-Mairipotaba;
it seems to be confined to the extensive layered ultramafics of Barro Alto and the Macedo-Niquelandia areas. The distribution
of Ni-values in the Brazilian plant collection is different from that found in the Mediterranean and California, where there
is a clear distinction between accumulator and non-accumulator plants: in Brazil the distribution is more continuous, and
median Ni concentrations are much greater. An ultramafic hill just north of Niquelandia deserves to be protected because of
the presence there of many of the hyperaccumulators and species probably endemic to the Goiás ultramafics. 相似文献
2.
Weber Igor Daniel Lopes Welinton Ribamar de Paula Letícia Chiara Baldassio Albernaz-Godinho Karina Cordeiro Czepak Cecilia 《BioControl》2021,66(5):625-637
BioControl - Lepidopterans are among the most important groups of pests for crops in central Brazil. Fortunately, there is a significant diversity of natural enemies acting in the natural... 相似文献
3.
Gadelha SR Shindo N Cruz JN Morgado MG Galvão-Castro B 《Memórias do Instituto Oswaldo Cruz》2003,98(4):461-463
We analyzed, by env and gag heteroduplex mobility assay, 149 human immunodeficiency virus (HIV-1) positive samples collected in Ceará during the year 2000. The prevalence of subtype B was 81.2% and the prevalence of subtype F and B/F recombinants were both 2.7%. Eight (5.4%) and 12 (8%) out of 149 samples showed indeterminate results in the env and gag analysis respectively. By FokI restriction fragment length polymorphism, 34% of the subtype B samples were identified as the typical Brazilian subtype B.In the present study, we identified HIV-1 subtype F and B/F in Ceará for the first time. Our results contribute to the understanding of HIV in Brazil, and may prove useful for the development of vaccine candidates. 相似文献
4.
Cardoso AM Martins de Sousa E Viana-Niero C Bonfim de Bortoli F Pereira das Neves ZC Leão SC Junqueira-Kipnis AP Kipnis A 《Microbes and infection / Institut Pasteur》2008,10(14-15):1552-1557
A cluster of surgical site infection cases after arthroscopic and laparoscopic procedures occurred between 2005 and 2007 in Goiania, in the central region of Brazil. Nontuberculous mycobacteria (NTM) were isolated from samples (exudates from cutaneous abscesses) from 18 patients of seven private hospitals. There were no reports of post-surgical arthroscopic and laparoscopic mycobacterial infections in Goiania apart from this period. The 18 isolates were identified as Mycobacterium massiliense by PCR-restriction digestion of the hsp65 gene, pulsed-field gel electrophoresis (PFGE) comparisons, and rpoB partial gene sequencing. All isolates were typed as a single clone, indicating that they have the same origin, which suggests a common source of infection for all patients. 相似文献
5.
In 1943, the Center for the Study and Prophylaxis of Chagas Disease, Oswaldo Cruz Foundation, state of Minas Gerais (MG), was created in the municipality of Bambuí to carry out studies related to Chagas disease in the mid-western region of MG. Since that time, several investigations have been conducted to determine the natural habitat of triatomines, but Panstrongylus megistus colonies have never been found in this region. This paper records the first finding of a P. megistus sylvatic colony in 69 years of research. The characteristics of this ecotope and its implications for the epidemiology of Chagas disease are discussed. 相似文献
6.
Costa IR Silva RC Frare AB Silva CT Bordin BM Souza SR Ribeiro Júnior CL Moura KK 《Genetics and molecular research : GMR》2011,10(3):1364-1370
We investigated a possible link between endometriosis and polymorphism of the progesterone receptor gene (PROGINS). The endometriosis group consisted of 54 patients with a diagnosis of endometriosis by laparoscopy, and the control group comprised 44 women without endometriosis. Genotypes for PROGINS polymorphisms (A1/A1, A1/A2 and A2/A2) were determined by polymerase chain reaction and analyzed on a 2% agarose gel stained with ethidium bromide. The frequency of polymorphic genotypes (A1/A2 and A2/A2) was significantly higher in patients with endometriosis (33%) than in the control group (16%). We conclude that there is a significant correlation between PROGINS polymorphism and endometriosis. 相似文献
7.
Bueno VF de Mesquita AJ Neves RB de Souza MA Ribeiro AR Nicolau ES de Oliveira AN 《Mycopathologia》2006,161(3):141-145
The purpose of this survey was to describe the occurrence of bovine mastitis caused by Prototheca zopfii in Goiás State, Brazil. Samples of milk, environment and udder were taken from a herd of 120 Holstein cows. Sabourauds dextrose
agar plates were incubated under aerobic conditions at 37 °C/96 h, for microbiological analysis. Somatic cell count and milk
composition were also determined. Histological sections from two udders were stained with HE and PAS. Prototheca zopfii was identified in six cows whose milk had a watery appearance. They also showed a pronounced decrease in milk yield, fat
and lactose. Pronounced infiltration of mononuclear cells, atrophy of alveoli and fibrosis were observed. The presence of
this agent in other herds in the State is highly likely. 相似文献
8.
9.
Cardoso Dd Soares CM Dias e Souza MB de Azevedo Mda S Martins RM Queiróz DA de Brito WM Munford V Rácz ML 《Memórias do Instituto Oswaldo Cruz》2003,98(1):25-29
A total of 2,605 faecal specimens from children up to 10 years old with or without diarrhoea were collected. Samples were obtained from 1986 to 2000 in hospitals, outpatient clinics and day-care centers in Goiania, Goiás. Two methodologies for viral detection were utilized: a combined enzyme immunoassay for rotavirus and adenovirus and polyacrylamide gel electrophoresis. Results showed 374 (14.4%) faecal specimens positive for Rotavirus A, most of them collected from hospitalized children. A significant detection rate of rotavirus during the period from April to August, dry season in Goiania, and different frequencies of viral detection throughout the years of study were also observed. Rotavirus was significantly related to hospitalization and to diarrhoeal illness in children up to 24 months old. This study reinforces the importance of rotavirus as a cause of diarrhoea in children and may be important in regards to the implementation of rotavirus vaccination strategies in our country. 相似文献
10.
Yamasaki T Duarte AM Curado I Summa ME Neves DV Wunderlich G Malafronte RS 《Journal of medical primatology》2011,40(6):392-400
Background In some states of the Brazilian extra‐Amazonian region, such as the Atlantic Forest area, autochthonous human cases of malaria were related to simian malarias and vice versa. Methods To verify the presence of Plasmodium, 50 blood samples of howler monkeys (Alouatta guariba clamitans) rescued from the Metropolitan Region of Saõ Paulo city, where the Atlantic Forest is present, were analyzed. The samples were submitted to microscopy (thin and thick blood smears), enzyme‐linked immunosorbent assays (ELISA), indirect immunofluorescent assay (IFA), and polymerase chain reaction (PCR). Results Only one smear showed forms reminiscent of Plasmodium vivax. In ELISA, the frequencies of antibodies against synthetic peptides corresponding to circumsporozoite protein of P. vivax VK210 ‘classic’ (Pvc), P. vivax VK247, human P. vivax‐like (Pvk and Pvl), P. malariae/P. brasilianum (Pm), and P. falciparum (Pf) were 24.0% (12/50) for Pvc, 8.0% (04/50) for Pvk, 6.0% (03/50) for Pvl, 24.0% (12/50) for Pm, and 28.0% (14/50) for Pf, while the frequency of antibodies against PvMSP119 recombinant proteins was 42.0% (21/50). No serum reacted against PfMSP1‐19. In IFA,the seropositivity of antibodies against asexual forms of P. malariae was 31.3% (15/48). We utilized three PCR protocols to develop a molecular consensus (positive results in, at least, two protocols). The frequency of Plasmodium infections detected by PCR was 18.0% (09/50) for P. vivax, 4.0% (02/50) for P. malariae, and 76.0% (38/50) of samples were negative. The molecular consensus was not seen in 4.0% (02/50) of samples. Conclusions These results suggest that a possible interaction between human and simian malaria coming from a zoonotic cycle cannot be discarded because simians that live in the areas of the Atlantic Forest could play a role as a reservoir for Plasmodium. 相似文献
11.
A total of 283 Salmonella Typhimurium strains isolated from cases of human infections and non human sources, were examined for antimicrobial susceptibility and the incidence of resistance was 38% and multiple resistance (to three or more antimicrobials) was 15%. All 43 multidrug-resistant strains (MDR) and 13 susceptible ones were characterized by phage typing and pulsed- field gel electrophoresis (PFGE). The strains encompassed 14 definitive phage types (DT), three were untypable (UT), and 18 atypicals or reaction does not conform (RDNC), which belonged to 21 PFGE patterns, A1-A21. The predominant phage types were DT49, DT193, and RDNC and two strains belonging to DT 104 and 104b were also identified. The most common PFGE patterns were A1 and A8. Analysis by PFGE and phage typing demonstrated that the most of the MDR were multiclonal and association among multiresistance, phage typing, and PFGE patterns was not so significant. 相似文献
12.
Velho L. F. M. Lansac-Tôha F. A. Bonecker C. C. Bini L. M. Rossa D. C. 《Hydrobiologia》2001,(1):385-391
The assessment of factors that determine the spatial distribution of zooplankton abundance is a central issue in zooplankton ecology. Since these factors are, in the most cases, spatially autocorrelated, especially in reservoirs, it is frequently difficult to isolate the primary causes that explain the variation in zooplankton abundance. In this paper, the spatial variation of copepods in Corumbá Reservoir (Goiás, Brazil) was studied. Sets of variables were used to model copepod abundance variation: limnology (abiotic variables), phytoplankton and spatial position of sampling sites. The copepod assemblage was composed of Thermocyclops minutus, Thermocyclops decipiens, Mesocyclops longisetus and Notodiaptomus iheringi. Along the reservoir's main axis, abundance increases, albeit not linearly, towards the lacustrine zone. High abundance, similar to that found in the lacustrine region, was also observed in the lateral arms. These patterns were very similar for different years and seasons. Copepods were significantly correlated with phytoplankton and geography. After controlling for the effect of space, the relationship between copepods and phytoplankton was not significant (partial Mantel test). This result indicates that hydrology is the primary process that controls not only the abundance of copepods, but also limnological features and food resources, which are commonly used to explain variations in zooplankton abundance. 相似文献
13.
Based on recent surveys of the freshwater decapod fauna, distributional data of five exotic species of freshwater decapod crustaceans for the hydrographic basins of the state of São Paulo are presented, as part of a large initiative for a comprehensive survey of the state’s biodiversity (BIOTA-FAPESP Program). These species are the North American crayfish Procambarus clarkii (Girard) (Cambaridae), the crab Dilocarcinus pagei Stimpson (Trichodactylidae) from the Amazon and Paraguay/lower Paraná River Basins, and the palaemonid shrimps Macrobrachium rosenbergii (De Man), from the Indo-Pacific region, Macrobrachium amazonicum (Heller) and Macrobrachium jelskii (Miers), both from the Orinoco, Amazon and the Paraguay/lower Paraná River Basins. Possible modes by which their introduction might have occurred are commented upon and potential consequences are discussed. 相似文献
14.
15.
To determine the polymorphisms of the prion protein gene in sheep from the state of Paraná, Brazil, 323 animals of meat breeds (Suffolk, Hampshire Down, Texel, Ile de France, Dorper, Dorset, Santa Inês and crossbreds) were genotyped by restriction fragment length polymorphism (RFLP) analysis. The most frequent allele was ARQ, with a frequency of 0.61, followed by ARR (0.30). VRQ and AHQ alleles were present at very low frequencies (0.13 and 0.05 respectively), and the ARH allele was not found. Seven genotypes were identified (ARR/ARR, ARR/ARQ, ARQ/ARQ, ARR/VRQ, ARR/AHQ, ARQ/VRQ and ARQ/AHQ), of which ARQ/ARQ was the most frequent (0.41). The Santa Inês breed and crossbred animals showed the highest genotypic variability. 相似文献
16.
Débora Dias Da Silva Lilian Berta Rihs Maria Da Luz Rosário de Sousa 《Gerodontology》2020,37(3):288-296
Objectives: The objective of this study was to verify the association between the number of teeth present with socio-demographic and economic variables and with the access to dental services and self-perception of oral health among the elderly. Materials and Methods: The sample was composed of individuals from 65 to 74 years of age, which was representative of the state of São Paulo (n = 781). In this study, the analysis of data considered dentate elderly only, who were divided into two groups: those with one to 19 teeth and those with 20 teeth or more. The chi-squared test was used in the bivariate analysis and the logistic regression was also performed (p < 0.05). Results: Among the 313 dentate elderly, 235 (75.1%) presented one to 19 teeth. The average number of teeth was 9.5 for the elderly with fewer teeth and 25 for those with more teeth. In the bivariate analysis, all variables related to the access to dental services were associated and the chances of having less teeth was among elderly who did not visit the dentist for a long time and among those who sought public dental services and did not receive information on how to avoid oral problems. In addition, the elderly who classified their speech as not good were those who presented with fewer teeth. Conclusion: Most subjects presented one to 19 teeth and this fact was directly associated, among others factors, to the unsatisfactory perception that they reported in relation to their speech and to the too long period without visiting the dentist. 相似文献
17.
Lorosa ES Valente MV Cunha V Lent H Jurberg J 《Memórias do Instituto Oswaldo Cruz》2003,98(7):885-887
Following the report of triatomine nymphs in a house in Arcadia, Miguel Pereira, state of Rio de Janeiro, Brazil, the infested dwelling was checked. Several eggs and 46 specimens of Triatoma vitticeps (Stal, 1859) were collected. Among them, adults and nymphal instars accounted for 43.5% and 56.5%, respectively. Analysis of blood meals showed the ecletism of this species; 24 (52.2%) were single feeds, 18 insects (39.1%) fed on two hosts and 4 (8.7%) on three hosts. Trypanosoma cruzi infection rate of examined specimens was 13%. Finally one of the residents of the house was positive for anti-T. cruzi antibodies using indirect immunofluorescence. 相似文献
18.
Alves SL Metzker FS Araújo-Filho JA Junqueira-Kipnis AP Kipnis A 《Memórias do Instituto Oswaldo Cruz》2011,106(6):655-661
Drug resistance is one of the major concerns regarding tuberculosis (TB) infection worldwide because it hampers control of the disease. Understanding the underlying mechanisms responsible for drug resistance development is of the highest importance. To investigate clinical data from drug-resistant TB patients at the Tropical Diseases Hospital, Goiás (GO), Brazil and to evaluate the molecular basis of rifampin (R) and isoniazid (H) resistance in Mycobacterium tuberculosis. Drug susceptibility testing was performed on 124 isolates from 100 patients and 24 isolates displayed resistance to R and/or H. Molecular analysis of drug resistance was performed by partial sequencing of the rpoB and katGgenes and analysis of the inhA promoter region. Similarity analysis of isolates was performed by 15 loci mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) typing. The molecular basis of drug resistance among the 24 isolates from 16 patients was confirmed in 18 isolates. Different susceptibility profiles among the isolates from the same individual were observed in five patients; using MIRU-VNTR, we have shown that those isolates were not genetically identical, with differences in one to three loci within the 15 analysed loci. Drug-resistant TB in GO is caused by M. tuberculosis strains with mutations in previously described sites of known genes and some patients harbour a mixed phenotype infection as a consequence of a single infective event; however, further and broader investigations are needed to support our findings. 相似文献
19.
Araújo-Coutinho CJ Bernardino TC Pires ES Esch LV Viviani AB Cavados CF 《Journal of invertebrate pathology》2011,107(1):11-15
The aim of this study was to investigate the naturally occurring bacteria from Culicidae larvae found in São Paulo state between 2006 and 2008 from the Tiete Ecological Park in the municipality of Caraguatatuba. Bacterial strains were obtained after surface sterilization of larvae followed by thermal treatment and incubation in bacteriological media under laboratory conditions. Identification was determined using cytomorphology, biochemical and physiological tests. Strains were characterized by qualitative evaluation of biological activity against Culicidae larvae, comparing protein profiles obtained by electrophoresis of crystal protoxins, electrophoresis of Lysinibacillus sphaericus isoenzymes, detection of the L2 fraction of Hemolytic BL enterotoxin and amplification of DNA using Multiplex-PCR to detect HBL, NHE, CytK, BceT and EntFM enterotoxins. The results obtained in this study demonstrated that the endosymbiotic bacterial diversity belonging to Bacillus and related genera associated with Culicidae larvae is limited to a number of species that does not vary throughout the seasons. 相似文献
20.
This study evaluates the relationships between rotifers and phytoplankton and rotifers and bacterioplankton in a tropical reservoir. Fourteen stations in the reservoir were sampled, including in its arms and tributaries, in the dry and rainy seasons. The highest rotifer density was found in the dry season, mainly in the upper and intermediary stretches of the reservoir. Brachionus calyciflorus, Polyarthra vulgaris, Keratella tropica, K. cochlearis, K. americana and Pompholyx complanata were the most abundant species. Densities of B. calyciflorus and bacteria were significantly correlated. On the other hand, there was an inverse relationship between P. vulgaris and bacteria. Diatoms (Bacillariophyceae) were observed to be associated to K. americana. We suggest that the rotifer populations play a part in microbial and herbivory food webs. 相似文献