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1.
A benthic algae sampling device designed for use through surface ice, uses the principle of pivoting arms. It is designed to be activated below ice sheets and is capable of passing easily through a hole of 11 cm diameter bored by a SIPRE ice auger. 相似文献
2.
André M. Comeau Benoît Philippe Mary Thaler Michel Gosselin Michel Poulin Connie Lovejoy 《Journal of phycology》2013,49(2):229-240
Global climate change is having profound impacts on polar ice with changes in the duration and extent of both land‐fast ice and drift ice, which is part of the polar ice pack. Sea ice is a distinct habitat and the morphologically identifiable sympagic community living within sea ice can be readily distinguished from pelagic species. Sympagic metazoa and diatoms have been studied extensively since they can be identified using microscopy techniques. However, non‐diatom eukaryotic cells living in ice have received much less attention despite taxa such as the dinoflagellate Polarella and the cercozoan Cryothecomonas being isolated from sea ice. Other small flagellates have also been reported, suggesting complex microbial food webs. Since smaller flagellates are fragile, often poorly preserved, and are difficult for non‐experts to identify, we applied high throughput tag sequencing of the V4 region of the 18S rRNA gene to investigate the eukaryotic microbiome within the ice. The sea ice communities were diverse (190 taxa) and included many heterotrophic and mixotrophic species. Dinoflagellates (43 taxa), diatoms (29 taxa) and cercozoans (12 taxa) accounted for ~80% of the sequences. The sympagic communities living within drift ice and land‐fast ice harbored taxonomically distinct communities and we highlight specific taxa of dinoflagellates and diatoms that may be indicators of land‐fast and drift ice. 相似文献
3.
《Bioscience, biotechnology, and biochemistry》2013,77(9):1950-1958
Some ice-nucleating bacterial strains, including Pantoea ananatis (Erwinia uredovora), Pseudomonas fluorescens, and Pseudomonas syringae isolates, were examined for the ability to shed ice nuclei into the growth medium. A novel ice-nucleating bacterium, Pseudomonas antarctica IN-74, was isolated from Ross Island, Antarctica. Cell-free ice nuclei from P. antarctica IN-74 were different from the conventional cell-free ice nuclei and showed a unique characterization. Cell-free ice nuclei were purified by centrifugation, filtration (0.45 μm), ultrafiltration, and gel filtration. In an ice-nucleating medium in 1 liter of cell culture, maximum growth was obtained with the production of 1.9 mg of cell-free ice nuclei. Ice nucleation activity in these cell-free ice nuclei preparations was extremely sensitive to pH. It was demonstrated that the components of cell-free ice nuclei were protein (33%), saccharide (12%), and lipid (55%), indicating that cell-free ice nuclei were lipoglycoproteins. Also, carbohydrate and lipid stains showed that cell-free ice nuclei contained both carbohydrate and lipid moieties. 相似文献
4.
Janne-Markus Rintala Jonna Piiparinen Jens Ehn Riitta Autio Harri Kuosa 《Hydrobiologia》2006,554(1):11-24
The response of Baltic Sea ice communities to changing light climate was studied in three subsequent 3 week in situ experiments on the SW coast of Finland. The investigation covered three different winter periods, short day with low solar
angles leading to limited light in the ice, late winter with deep snow cover and early spring with melting snow and increasing
light availability. The experimental setup consisted of transparent (no snow) and completely darkened (heavy snow cover) plexiglass
tubes in which the ice cores were incubated in situ from 1 to 2 weeks. Changes in the concentrations of inorganic nutrients (NO3−-–N, PO43−-–P, SiO4−-–Si) and chlorophyll-a concentration in the phytoplankton community composition were recorded as responses to different light manipulations. Changes
in inner ice light intensity in untreated ice as well as the temperature both in air and ice were recorded over the entire
study period. Increased irradiance in late winter/early spring and during meltdown affected the chlorophyll-a amount in the sea ice. During these periods the phytoplankton community in the top layers decreased possibly as a consequence
of photo-acclimation. Closer to the bottom of the ice, however, the increased inner ice light intensity induced algal growth.
Complete exclusion of light stopped the algal growth in the whole ice column. Darkening the ice cores also slowed down the
ice melting opposite to accelerated melting caused by increased light. The significant differences found in nutrient concentrations
between the light and dark treatments were mostly explicable by changes in algal biomass. No obvious changes were observed
in the phytoplankton community composition due to light manipulation, diatoms and heterotrophic flagellates dominating throughout
the study period. 相似文献
5.
Ben Raymond K. Meiners C. W. Fowler B. Pasquer G. D. Williams S. Nicol 《Polar Biology》2009,32(3):443-452
We present a computational model of the large-scale cumulative light exposure of sea ice in the Southern Ocean off East Antarctica
(30°E–150°E). The model uses remotely sensed or modelled sea ice concentration, snow depth over sea ice, and solar irradiance
data, and tracks sea ice motion over the season of interest in order to calculate the cumulative exposure of the ice field
to photosynthetically active radiation (PAR). Light is the limiting factor to sea ice algal growth over winter and early spring,
and so the results have implications for the estimation of algal biomass in East Antarctica. The model results indicate that
highly light-exposed ice is restricted to within a few degrees of the coast in the eastern part of the study region, but extends
much further north in the 30°E–100°E sector. The relative influences of sea ice motion, solar flux, and snow depth variations
on interannual variations in model predictions were evaluated. The model estimates of cumulative PAR were found to correlate
with satellite estimates of subsequent open-water chlorophyll-a concentration, consistent with the notion that sea ice algae can provide inocula for phytoplankton blooms. 相似文献
6.
Michael G. Janech Andreas Krell Thomas Mock Jae‐Shin Kang James A. Raymond 《Journal of phycology》2006,42(2):410-416
Sea ice diatoms thrive under conditions of low temperature and high salinity, and as a result are responsible for a significant fraction of polar photosynthesis. Their success may be owing in part to secretion of macromolecules that have previously been shown to interfere with the growth of ice and to have the ability to act as cryoprotectants. Here we show that one of these molecules, produced by the sea ice diatom Navicula glaciei Vanheurk, is a ~25 kDa ice‐binding protein (IBP). A cDNA obtained from another sea ice diatom, Fragilariopsis cylindrus Grunow, was found to encode a protein that closely matched the partially sequenced N. glaciei IBP, and enabled the amplification and sequencing of an N. glaciei IBP cDNA. Similar proteins are not present in the genome of the mesophilic diatom Thalassiosira pseudonana. Both proteins closely resemble antifreeze proteins from psychrophilic snow molds, and as a group represent a new class of IBPs that is distinct from other IBPs found in fish, insects and plants, and bacteria. The diatom IBPs also have striking similarities to three prokaryotic hypothetical proteins. Relatives of both snow molds and two of the prokaryotes have been found in sea ice, raising the possibility of a fungal or bacterial origin of diatom IBPs. 相似文献
7.
Climate warming is causing unidirectional changes to annual patterns of sea ice distribution, structure, and freeze‐up. We summarize evidence that documents how loss of sea ice, the primary habitat of polar bears (Ursus maritimus), negatively affects their long‐term survival. To maintain viable subpopulations, polar bears depend on sea ice as a platform from which to hunt seals for long enough each year to accumulate sufficient energy (fat) to survive periods when seals are unavailable. Less time to access to prey, because of progressively earlier breakup in spring, when newly weaned ringed seal (Pusa hispida) young are available, results in longer periods of fasting, lower body condition, decreased access to denning areas, fewer and smaller cubs, lower survival of cubs as well as bears of other age classes and, finally, subpopulation decline toward eventual extirpation. The chronology of climate‐driven changes will vary between subpopulations, with quantifiable negative effects being documented first in the more southerly subpopulations, such as those in Hudson Bay or the southern Beaufort Sea. As the bears' body condition declines, more seek alternate food resources so the frequency of conflicts between bears and humans increases. In the most northerly areas, thick multiyear ice, through which little light penetrates to stimulate biological growth on the underside, will be replaced by annual ice, which facilitates greater productivity and may create habitat more favorable to polar bears over continental shelf areas in the short term. If the climate continues to warm and eliminate sea ice as predicted, polar bears will largely disappear from the southern portions of their range by mid‐century. They may persist in the northern Canadian Arctic Islands and northern Greenland for the foreseeable future, but their long‐term viability, with a much reduced global population size in a remnant of their former range, is uncertain. 相似文献
8.
Justin A. Crawford Kathryn J. Frost Lori T. Quakenbush Alex Whiting 《Polar Biology》2012,35(2):241-255
Habitat partitioning by adult and subadult ringed seals (Phoca hispida) is poorly understood. Conclusions about displacement of subadult seals to suboptimal offshore habitat are largely based
on nearshore observations as few satellite tagging studies include data from winter months. In this study, movement patterns
of 14 subadult and 11 adult ringed seals were monitored in the Bering and Chukchi seas using satellite-linked telemetry. Seals
were captured in Kotzebue Sound, Alaska, during October 2007 and 2008 and tracked for 17–297 days. Subadult ringed seals traveled
south from the Chukchi Sea into the Bering Sea ([`(x)] \bar{x} = 36 km/day) as sea ice coverage increased during November and December, remained ~1,000 km south near the ice edge during
winter and returned north in the spring with the receding ice edge. Adults remained in the Chukchi and northern Bering seas,
where their movements were more localized ([`(x)] \bar{x} = 22 km/day). Adults were on average 322 km farther from the ice edge and 48 km closer to land and shorefast ice than were
subadults. During winter, adult ringed seals construct and maintain breathing holes through the ice, and in spring, females
give birth in subnivean lairs, mostly in shorefast ice; adult males defend breeding territories around those lairs. Our results
show that subadult ringed seals, unconstrained by the need to maintain territories that contain stable breeding/pupping habitat,
moved south to the Bering Sea ice edge, where there are better feeding opportunities, lower energetic costs (no breathing
hole maintenance), and less exposure to predation. 相似文献
9.
Gilbert Neuner Benjamin Kreische Dominik Kaplenig Kristina Monitzer Ramona Miller 《Plant, cell & environment》2019,42(7):2065-2074
The frost survival mechanism of vegetative buds of angiosperms was suggested to be extracellular freezing causing dehydration, elevated osmotic potential to prevent freezing. However, extreme dehydration would be needed to avoid freezing at the temperatures down to ?45°C encountered by many trees. Buds of Alnus alnobetula, in common with other frost hardy angiosperms, excrete a lipophilic substance, whose functional role remains unclear. Freezing of buds was studied by infrared thermography, psychrometry, and cryomicroscopy. Buds of A. alnobetula did not survive by extracellular ice tolerance but by deep supercooling, down to ?45°C. An internal ice barrier prevented ice penetration from the frozen stem into the bud. Cryomicroscopy revealed a new freezing mechanism. Until now, supercooled buds lost water towards ice masses that form in the subtending stem and/or bud scales. In A. alnobetula, ice forms harmlessly inside the bud between the supercooled leaves. This would immediately trigger intracellular freezing and kill the supercooled bud in other species. In A. alnobetula, lipophilic substances (triterpenoids and flavonoid aglycones) impregnate the surface of bud leaves. These prevent extrinsic ice nucleation so allowing supercooling. This suggests a means to protect forestry and agricultural crops from extrinsic ice nucleation allowing transient supercooling during night frosts. 相似文献
10.
Başyiğit G Kuleaşan H Karahan AG 《Journal of industrial microbiology & biotechnology》2006,33(9):796-800
A mixture of human-derived probiotic strains of Lactobacillus acidophilus, L. agilis and L. rhamnosus was used as a probiotic culture in ice cream manufacture. Viability and survival of these probiotic cultures were investigated in two different ice cream formulations. Ice cream with sucrose and ice cream with aspartame were prepared and each of these was divided into two subgroups: one with direct addition of the probiotic culture and one with milk fermented by the same probiotic culture. Ice cream samples were stored at −20°C for 6 months and the survival rate of cultures were determined monthly. Probiotic cultures underwent tests for resistance to bile salts, antibiotics, acidic conditions; they were found to be highly resistant to such challenges. Chemical analysis of ice cream samples, such as determination of acidity, pH and solid matter, was also performed. The probiotic cultures remained unchanged in ice cream stored for up to 6 months regardless of the sweeteners used. Using probiotic cultures in ice cream mixes did not alter the characteristics of the product. 相似文献
11.
Thin sections of laminated cores from different Antarctic coastal areas have demonstrated the potential of diatom species to document climate change at the seasonal scale. Here we present the relative abundances of four diatom species and species groups (Fragilariopsis curta group as a proxy for yearly sea ice cover, F. kerguelensis as a proxy for summer sea-surface temperature, Chaetoceros Hyalochaete resting spores as a proxy for spring sea ice melting and the Thalassiosira antarctica group as a proxy for autumn sea ice formation) in core MD03-2601 retrieved off Adélie Land on the Antarctic continental shelf. These abundances were compared to surface temperatures and sea ice cover modelled over the last 9000 years. Both the marine records and the simulated climate demonstrated a cooler Early Holocene (9000–7700 years BP), a warmer Mid-Holocene (7700–4000 years BP) and a colder Late Holocene (4000–1000 years BP). Yearly sea ice cover followed an inverse pattern to temperatures with less sea ice during the Mid-Holocene Hypsithermal than during the Late Holocene Neoglacial. However, diatom census counts and model output indicate that sea ice spring melting happened earlier in the season, as expected, but that autumn sea ice formation also occurred earlier in the season during the Hypsithermal than during the colder Neoglacial, thereby following seasonal changes in local insolation. 相似文献
12.
The impacts of ultraviolet‐B radiation (UVB) on polar sea‐ice algal communities have not yet been demonstrated. We assess the impacts of UV on these communities using both laboratory experiments on algal isolates and by modification of the in situ spectral distribution of the under‐ice irradiance. In the latter experiment, filters were attached to the upper surface of the ice so that the algae were exposed in situ to treatments of ambient levels of PAR and UV radiation, ambient radiation minus UVB, and ambient radiation minus all UV. After 16 d, significant increases in chl a and cell numbers were recorded for all treatments, but there were no significant differences among the different treatments. Bottom‐ice algae exposed in vitro were considerably less tolerant to UVB than those in situ, but this tolerance improved when algae were retained within a solid block of ice. In addition, algae extracted from brine channels in the upper meter of sea ice and exposed to PAR and UVB in the laboratory were much more tolerant of high UVB doses than were any bottom‐ice isolates. This finding indicates that brine algae may be better adapted to high PAR and UVB than are bottom‐ice algae. The data indicate that the impact of increased levels of UVB resulting from springtime ozone depletion on Antarctic bottom‐ice communities is likely to be minimal. These algae are likely protected by strong UVB attenuation by the overlying ice and snow, by other inorganic and organic substances in the ice matrix, and by algal cells closer to the surface. 相似文献
13.
Jennifer Pike Xavier Crosta Eleanor J. Maddison Catherine E. Stickley Delphine Denis Loïc Barbara Hans Renssen 《Marine Micropaleontology》2009,73(1-2):14-25
The available ecological and palaeoecological information for two sea ice-related marine diatoms (Bacillariophyceae), Thalassiosira antarctica Comber and Porosira glacialis (Grunow) Jørgensen, suggests that these two species have similar sea surface temperature (SST), sea surface salinity (SSS) and sea ice proximity preferences. From phytoplankton observations, both are described as summer or autumn bloom species, commonly found in low SST waters associated with sea ice, although rarely within the ice. Both species form resting spores (RS) as irradiance decreases, SST falls and SSS increases in response to freezing ice in autumn. Recent work analysing late Quaternary seasonally laminated diatom ooze from coastal Antarctic sites has revealed that sub-laminae dominated either by T. antarctica RS, or by P. glacialis RS, are nearly always deposited as the last sediment increment of the year, interpreted as representing autumn flux. In this study, we focus on sites from the East Antarctic margin and show that there is a spatial and temporal separation in whether T. antarctica RS or P. glacialis RS form the autumnal sub-laminae. For instance, in deglacial sediments from the Mertz Ninnis Trough (George V Coast) P. glacialis RS form the sub-laminae whereas in similar age sediments from Iceberg Alley (Mac.Robertson Shelf) T. antarctica RS dominate the autumn sub-lamina. In the Dumont d'Urville Trough (Adélie Land), mid-Holocene (Hypsithermal warm period) autumnal sub-laminae are dominated by T. antarctica RS whereas late Holocene (Neoglacial cool period) sub-laminae are dominated by P. glacialis RS. These observations from late Quaternary seasonally laminated sediments would appear to indicate that P. glacialis prefers slightly cooler ocean–climate conditions than T. antarctica. We test this relationship against two down-core Holocene quantitative diatom abundance records from Dumont d'Urville Trough and Svenner Channel (Princess Elizabeth Land) and compare the results with SST and sea ice concentration results of an Antarctic and Southern Ocean Holocene climate simulation that used a coupled atmosphere–sea ice–vegation model forced with orbital parameters and greenhouse gas concentrations. We find that abundance of P. glacialis RS is favoured by higher winter and spring sea ice concentrations and that a climatically-sensitive threshold exists between the abundance of P. glacialis RS and T. antarctica RS in the sediments. An increase to > 0.1 for the ratio of P. glacialis RS:T. antarctica RS indicates a change to increased winter sea ice concentration (to >80% concentration), cooler spring seasons with increased sea ice, slightly warmer autumn seasons with less sea ice and a change from ~ 7.5 months annual sea ice cover at a site to much greater than 7.5 months. In the East Antarctic sediment record, an increase in the ratio from <0.1 to above 0.1 occurs at the transition from the warmer Hypsithermal climate into the cooler Neoglacial climate (~ 4 cal kyr) indicating that the ratio between these two diatoms has the potential to be used as a semi-quantitative climate proxy. 相似文献
14.
Dependency of Antarctic zooplankton species on ice algae‐produced carbon suggests a sea ice‐driven pelagic ecosystem during winter
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Doreen Kohlbach Martin Graeve Benjamin A. Lange Carmen David Fokje L. Schaafsma Jan Andries van Franeker Martina Vortkamp Angelika Brandt Hauke Flores 《Global Change Biology》2018,24(10):4667-4681
How the abundant pelagic life of the Southern Ocean survives winter darkness, when the sea is covered by pack ice and phytoplankton production is nearly zero, is poorly understood. Ice‐associated (“sympagic”) microalgae could serve as a high‐quality carbon source during winter, but their significance in the food web is so far unquantified. To better understand the importance of ice algae‐produced carbon for the overwintering of Antarctic organisms, we investigated fatty acid (FA) and stable isotope compositions of 10 zooplankton species, and their potential sympagic and pelagic carbon sources. FA‐specific carbon stable isotope compositions were used in stable isotope mixing models to quantify the contribution of ice algae‐produced carbon (αIce) to the body carbon of each species. Mean αIce estimates ranged from 4% to 67%, with large variations between species and depending on the FA used for the modelling. Integrating the αIce estimates from all models, the sympagic amphipod Eusirus laticarpus was the most dependent on ice algal carbon (αIce: 54%–67%), and the salp Salpa thompsoni showed the least dependency on ice algal carbon (αIce: 8%–40%). Differences in αIce estimates between FAs associated with short‐term vs. long‐term lipid pools suggested an increasing importance of ice algal carbon for many species as the winter season progressed. In the abundant winter‐active copepod Calanus propinquus, mean αIce reached more than 50% in late winter. The trophic carbon flux from ice algae into this copepod was between 3 and 5 mg C m?2 day?1. This indicates that copepods and other ice‐dependent zooplankton species transfer significant amounts of carbon from ice algae into the pelagic system, where it fuels the food web, the biological carbon pump and elemental cycling. Understanding the role of ice algae‐produced carbon in these processes will be the key to predictions of the impact of future sea ice decline on Antarctic ecosystem functioning. 相似文献
15.
Morten Stickler Knut Alfredsen David A. Scruton Curtis Pennell Atle Harby Finn Økland 《Hydrobiologia》2007,582(1):81-89
A telemetry study in a Norwegian regulated river was conducted through a 12-day period in mid-winter 2003. The objective was
to study activity (defined as number of movement per hour) and movement (defined as distance moved per hour) during different
ice formation events. Twenty-four Atlantic salmon (Salmo salar L.) parr were radio tagged and continuously monitored by both manually tracking (N = 24) and by fixed recording stations (N = 15). Detailed data on climate, flow and ice formation and its spatial distribution were collected and used in the analyses.
Fish activity was not found to be affected by their size (L
F). There was a significant difference in activity between diel periods with highest activity during dusk (5–6 p.m.). Between
high and low flow (mean ± SD, 21.1 m3 s−1 ± 1.7 SD and 11.1 m3 s−1 ± 1.7 SD, respectively) no significant difference in activity was found. During the experiment extensive anchor ice growth
occurred mainly in the riffle part with thickness up to 50 cm. Juveniles tend to avoid riffle section during anchor ice formation
and exploited ice covered areas, indicating critical and preferable habitats respectively. Further, a significant difference
in movement was found between five selected ice events with highest mean movement during an anchor ice event and lowest mean
movement during an ice break up with no anchor ice formation. No significant difference in activity or movement between parr
exposed to frazil ice and parr not exposed were found. 相似文献
16.
During spring, extensive blooms of microalgae grow on the underside of arctic sea ice. The brownish, algal layer penetrates ca. 2 cm into the bottom surface of the ice and the algae are potentially exposed to very high salinities. Four diatom species, Melosira juergensii Ag., Porosira glacialis (Grun.) Jørg., Navicula transitans var. derasa (Grun.) Cleve, and Coscinodiscus lacustris Grun., isolated from, sea ice samples taken from the Beaufort and Chukchi seas near Barrow, Alaska, were grown at 11 salinities ranging from 5 to 70‰ at 5 C under constant illumination. All of the species grew at 5‰ except N. transitans whose lower growth limit was 15‰. Growth was high over a broad range of salinities, but none of the species grew at salinities above 60‰. These diatom species appear to be well suited to tolerate the salinities in the brine pockets near the bottom of annual arctic sea ice where they are found. High brine-cell salinity, however, may limit the upward, penetration of ice algae into the bottom of sea ice. 相似文献
17.
Lisa G. Neven John G. Duman Martin G. Low Louis C. Sehl Francis J. Castellino 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1989,159(1):71-82
Summary A lipoprotein with ice nucleator activity was purified from the hemolymph of the freezetolerant larvae of the craneflyTipula trivittata. Characterization of this lipoprotein ice nucleator (LPIN) showed that it differed from other previously described insect hemolymph lipoproteins which lack ice nucleator activity, by the presence of phosphatidylinositol (PI) at 11.0% by weight of the total phospholipid content. The potential roles of PI and other lipoprotein components in the ice nucleating activity were examined using various phospholipases, proteases, LPIN antibodies, borate compounds and various lipid-protein reconstitutions. It was found that phosphatidylinositol specific phospholipase C was the most effective phospholipase in eliminating the activity of the LPIN. Borate compounds effectively depressed activity. Treatment of the LPIN with protease also eliminated ice nucleator activity but the binding of LPIN specific antibody did not. Reconstitutions consisting of the native LPIN lipids, PI specific phospholipase-treated native LPIN lipids, or pure standard phospholipids with the apolipoproteins of the LPIN andManduca sexta larval lipoproteins gave evidence that both the apolipoproteins of the LPIN and PI are necessary for the ice nucleating activity.Abbreviations LPIN
polyclonal antibodies to lipoprotein ice nucleator
-
ANOVA
analysis of variance
-
Apo-I
apolipoprotein I
-
Apo-II
apolipoprotein II
-
LPIN
lipoprotein ice nucleator
-
PAGE
polyacrylamide gel electrophoresis
-
PAS
Periodoacetate-Schiff's base
-
PC
phosphatidylcholine
-
PE
phosphatidylethanolamine
-
PI
phosphatidylinositol
-
SCP
supercooling point (ice nucleation temperature)
-
SDS
sodium dodecyl sulfate
-
SDS-PAGE
sodium dodecyl sulfate polyacrylamide gel electrophoresis
-
TLC
thin layer chromatography 相似文献
18.
Direct cell injury in cryosurgery is highly related to intracellular ice formation (IIF) during tissue freezing and thawing. Mechanistic understanding of IIF in tumor cells is critical to the development of tumor cryo-ablation protocol. In aid of a high speed CMOS camera system, the events of IIF in MCF-7 cells have been studied using cryomicroscopy. Images of ‘darkening’ type IIF and recrystallization are compared between cells frozen with and without ice seeding. It is found that ice seeding has significant impact on the occurrence and growth of intracellular ice. Without ice seeding, IIF is observed to occur over a very small range of temperature (∼1 °C). The crystal dendrites are indistinguishable, which is independent of the cooling rate. Ice crystal grows much faster and covers the whole intracellular space in comparison to that with ice seeding, which ice stops growing near the cellular nucleus. Recrystallization is observed at the temperature from −13 °C to −9 °C during thawing. On the contrary, IIF occurs from −7 °C to −20 °C with ice seeding at a high subzero temperature (i.e., −2.5 °C). The morphology of intracellular ice frozen is greatly affected by the cooling rate, and no ‘darkening’ type ice formed inside cells during thawing. In addition, the intracellular ice formation is directional, which starts from the plasma membrane and grows toward the cellular nucleus with or without ice seeding. These results can be used to explain some findings of tumor cryosurgery in vivo, especially the causes of insufficient killing of tumor cells in the peripheral area near vessels. 相似文献
19.
R. L. Kepner Jr. R. A. Wharton Jr. R. D. Collier C. S. Cockell W. H. Jeffrey 《Hydrobiologia》2000,427(1):155-165
High-resolution spectral scans of solar ultraviolet radiation (UVR) were obtained directly beneath the 4.0–5.0 m thick, perennial ice cover of Lake Hoare, South Victoria Land, Antarctica. Both UVA (320–400 nm) and UVB (280–320 nm) radiation were detectable beneath the ice using a diver-deployed, underwater scanning spectroradiometer which permitted accurate measurement in the 280–340 nm range, while avoiding effects of surface shading and/or hole effects. UVR at wavelengths <310 nm was not detectable below the ice. This lower wavelength UVB appears to penetrate the Lake Hoare ice to depths of no more than 1.5 m during relatively cloud-free austral summer days. Based upon estimated biologically effective UVR dosages and DNA dosimeter data, exposure of benthic and planktonic microbes to the UVR encountered immediately beneath the ice is unlikely to inhibit microbial metabolism. Although waters of oligotrophic antarctic lakes are highly transparent to UVR, the thick, high scattering and optically dense ice covers on many of these lakes offers organisms a degree of protection largely unavailable in temperate and tropical systems. Thinning or complete loss of these overlying ice covers is likely to have major consequences for the structure of antarctic lake microbial communities. 相似文献
20.
Preliminary investigation of Okhotsk Sea ice algae; taxonomic composition and photosynthetic activity 总被引:1,自引:1,他引:0
Okhotsk Sea pack ice from Shiretoko in northern Hokkaido, sampled in March 2007, contained microalgal communities dominated
by the centric diatoms Thalassiosira
nordenskioeldi and T. punctigera. Domination by this genus is very unusual in sea ice. Communities from nearby fast ice at Saroma-ko lagoon were dominated by Detonula conferavea and Odontella aurita. Average microalgal biomass of the Okhotsk Sea pack ice (surface and bottom) was 1.59 ± 1.09 μg chla l−1 and for fast ice (bottom only) at nearby Saroma-ko lagoon, 16.5 ± 3.2 μg l−1 (=31.1 ± 5.0 mg chla m−2). Maximum quantum yield of the Shiretoko pack ice algal communities was 0.618 ± 0.056 with species-specific data ranging
between 0.211 and 0.653. These community values are amongst the highest recorded for sea ice algae. Rapid light curves (RLC) on individual cells indicated maximum relative electron transfer rates (relETR) between 20.8 and
60.6, photosynthetic efficiency values (α) between 0.31 and 0.93 and onset of saturation values (E
k) between 33 and 91 μmol photons m−2 s−1. These data imply that the pack ice algal community at Shiretoko was healthy and actively photosynthesising. Maximum quantum
yield of the Saroma-ko fast ice community was 0.401 ± 0.086, with values for different species between 0.361 and 0.560. RLC
data from individual Saroma-ko fast ice algal cells indicated relETR between 55.3 and 60.6, α values between 0.609 and 0.816
and E
k values between 74 and 91 μmol photons m−2 s−1 which are consistent with measurements in previous years. 相似文献