共查询到20条相似文献，搜索用时 46 毫秒
1.
α-
Jean-Paul Mornon Dyna Halaby Marc Malfois Patrick Durand Isabelle Callebaut Annette Tardieu 《International journal of biological macromolecules》1998,22(3-4)
New results obtained from a two-dimensional sequence analysis of the small heat shock protein (shsp) family are described. It is confirmed that the conserved C-terminal α-crystallin domain is essentially made of β-strands, most probably two groups of β-strands separated by a large loop. A direct correspondence between the putative β-strands that have been identified in shsps and the seven β-strands of a classical immunoglobulin-like fold is proposed. The hypothesis that the shsp family could belong to the immunoglobulin superfamily (IgSF) is consistent with the ubiquitous distribution and the multifunctional properties of the crystallins that are now emerging. 相似文献
2.
3.
Determination of
Ichiro Ono Keizo Matsuda Sachiko Kanno 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1997,692(2)
A simple, rapid and sensitive two column-switching high-performance liquid chromatographic (HPLC) method with ultraviolet detection at 210 nm has been developed for the determination of
(AY4166, I) and its seven metabolites in human plasma and urine. Measurements of I and its metabolites were carried out by two column-switching HPLC, because metabolites were classified into two groups according to their retention times. After purification of plasma samples using solid-phase extraction and direct dilution of urinary samples, I and each metabolite were injected into HPLC. The calibration graphs for plasma and urinary samples were linear in the ranges 0.1 to 10 μg ml−1 and 0.5 to 50 μg ml−1, respectively. Recoveries of I and its seven metabolites were over 88% by the standard addition method and the relative standard deviations of I and its metabolites were 1–6%. 相似文献
Full-size image
4.
5.
Synthesis of methyl
-Ribose was converted into methyl
and this, on tin-mediated allylation, gave a mixture of the 2-O-allyl and 3-O-allyl derivatives which were separated by chromatography. The more polar isomer was characterised as the 3-O-allyl derivative after conversion via
(which was also synthesised from
) into the known
. Methyl
was converted into methyl
via methyl
. 相似文献
Full-size image
Full-size image
Full-size image
Full-size image
Full-size image
Full-size image
6.
7.
8.
9.
10.
11.
12.
Cytogenetic evaluation of occupational exposure to external γ-rays and internal
M. Bauchinger E. Schmid H. Braselmann 《Mutation Research - Genetic Toxicology and Environmental Mutagenesis》1997,395(2-3)
Chromosome analyses were performed on peripheral lymphocytes from seven radiation workers exposed to external γ-radiation within maximum annual permissible dose limits during 11 to 22 years of employment. Five years prior to blood sampling, six workers had additionally incorporated the α-emitting radionuclide
which contributed between five to 25% of the total accumulated whole body dose in five workers and about 70% in one worker. For the radiation workers as a group, both the mean frequencies of symmetrical translocations measured by fluorescence in situ hybridisation (FISH)/chromosome painting and dicentrics+ring chromosomes scored in first division metaphases of conventional preparations were significantly elevated compared with respective controls. The mean biodosimetry estimate for the group was 270 mSv when based on stable translocations which compares well with the mean dose of 247 mSv based on official dosimetry records. The lower mean dose estimate of 160 mSv based on unstable dicentrics is compatible with the well-known loss of dicentrics from the circulating blood with time after exposure. 相似文献
Full-size image
13.
17.
18.
Manual and automated determination of
Daisy B. Whigan Alan E. Schuster 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1995,664(2)
This paper describes the determination of
and its metabolite (E)-5-(2-bromovinyl)uracil in urine. The method involves sample clean-up by liquid-liquid extraction with ethyl acetate followed by high-performance liquid chromatographic (HPLC) analysis. The sample preparation may be performed either manually or automatically using a Zymark Py-robotic system. The chloro analog of the parent compound, CV-araU, is used as the internal standard. As low as 0.1 μg of analyte per ml of urine can be measured. This sensitivity is adequate for pharmacokinetic studies but could be improved quite easily if necessary. 相似文献
Full-size image
19.
20.