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Making muscle in mammals. 总被引:22,自引:0,他引:22
M Buckingham 《Trends in genetics : TIG》1992,8(4):144-148
Classical embryology has provided a conceptual basis for our understanding of where muscle comes from. Histological and morphological studies of muscle fibre formation in the foetus and neonate have provided information on how muscle matures. More recent advances in molecular genetics have led to the characterization of muscle structural genes, and to the striking discovery of the MyoD family of myogenic regulatory factors. The question of how myogenesis takes place can now be formulated in terms of gene regulation, and molecular tools can be used to describe this process in the embryo and foetus. 相似文献
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Biochemistry of dystrophic muscle. 2. Some enzyme changes in dystrophic mouse muscle 总被引:3,自引:0,他引:3
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Pennington RJ 《The Biochemical journal》1963,88(1):64-68
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Dystrophin-deficient muscle undergoes sudden, postnatal onset of muscle necrosis that is either progressive, as in Duchenne muscular dystrophy, or successfully arrested and followed by regeneration, as in most muscles of mdx mice. The mechanisms regulating regeneration in mdx muscle are unknown, although the possibility that there is renewed expression of genes regulating embryonic muscle cell proliferation and differentiation may provide testable hypotheses. Here, we examine the possibility that necrotic and regenerating mdx muscles exhibit renewed or increased expression of PDGF-receptors. PDGF-binding to receptors on muscle has been shown previously to be associated with myogenic cell proliferation and delay of muscle differentiation. We find that PDGF-receptors are present in 4-week-old mdx mice in muscles that undergo brief, reversible necrosis (hindlimb muscles) or progressive necrosis (diaphragm), as well as in 4-week-old control mouse muscles. Immunoblots indicate that the concentrations of PDGF-receptors in 4-week-old dystrophic (necrotic) and control muscles are similar. Prenecrotic, dystrophic fibers and control fibers possess some cell surface labeling of fibers treated with anti-PDGF-receptor and viewed by indirect immunofluorescence. Necrotic fibers in dystrophic muscle show cytoplasmic labeling for PDGF-receptors and labeling of perinuclear regions at the muscle cell surface. Adult dystrophic muscle displays higher concentrations of PDGF-receptor in both regenerated muscle (hindlimb) and progressively necrotic muscle (diaphragm) than found in controls. Anti-PDGF-receptor labeling of regenerated, dystrophic muscle is observed primarily in granules surrounding central nuclei or surrounding nuclei located at the surface of regenerated fibers. No labeling of perinuclear regions of control muscle or prenecrotic fibers was observed. Myonuclei fractionated from adult mdx hindlimb muscles contained no PDGF-receptor, indicating that PDGF-receptor-positive structures are not tightly associated with nuclei or within nuclei. L6 myoblasts show PDGF-receptor distributed diffusely on the cell surface. Stimulation of L6 myoblasts with 10 ng/ml of PDGF-BB causes receptor internalization and concentration in granules at perinuclear regions. Thus, PDGF stimulation of myoblasts causes a redistribution of PDGF-receptors to resemble receptor localization observed during muscle regeneration. These findings implicate PDGF-mediated mechanisms in regeneration of dystrophic muscle. 相似文献
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J F Aronson 《Developmental biology》1976,48(2):487-492
The number of sarcomeres in a given muscle of the mite Tarsonemus randsi was constant in both larval and adult stages, with the exception of the two medial dorsal metapodosomal muscles in males. These muscles have three sarcomeres in larvae and one sarcomere in adults. This change in sarcomere number within a muscle was observed in the living animal by polarized light microscopy using parthenogenetically derived male larvae. Initially the transforming muscles shortened slowly (hours) and the appearance of the sarcomeres was comparable to that seen during normal contraction. With continued shortening there was apposition of adjacent A bands and disappearance of clearly visible Z lines, but no loss of birefringence. Over the next 12 hr there was further shortening of the muscle and loss of birefringence. This was apparent as shortening of the three apposed A regions to the length of a single A band with a small increase in muscle width and no increase in the peak retardation of the birefringent region. The observations are discussed in terms of differential loss of the A filaments of the two terminal sarcomeres. 相似文献
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A J Buller R Pope 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》1977,278(961):295-305
While it has been recognized for many years that different limb muscles belonging to the same mammal may have markedly differing contractile characteristics, it is only comparatively recently that it has been demonstrated that these differences depend upon the motor innervation. By appropriately changing the peripheral nerve innervating a mammalian skeletal muscle, it is possible to change dramatically the contractile behaviour of the reinnervated muscle. The manner by which the motor innervation determines the nature of a muscle fibre's contractile machinery is not completely understood, but it appears that the number and pattern of motor nerve impulses reaching the muscle play an important role. The biochemical changes occurring within muscle fibres whose contractile properties have been modified by altered motor innervation include the synthesis of different contractile proteins. 相似文献
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Surface features of striated muscle. II. Guinea-pig skeletal muscle 总被引:12,自引:0,他引:12
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Chicken cardiac muscle myofibrils lack a visible M-line. Antibodies against chicken breast muscle M-protein, an M-line component with Mr = 165 000, were used to demonstrate the presence of a similar protein in chicken heart muscle. The immunoreplica technique showed the heart protein to have about the same molecular weight as the breast muscle M-protein on polyacrylamide slab gels in the presence of sodium dodecyl sulfate (SDS). Positive staining within the H-zone was observed when the indirect immunofluorescence technique was used to localize the M-protein in isolated heart myofibrils. This result was confirmed by electron microscopic investigations on longitudinal sections of antibody-incubated heart muscle fiber bundles showing the antibody against M-protein to be bound within a region corresponding to the M-line region of breast muscle myofibrils. 相似文献
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A recent study identifies a novel nonautonomous signaling pathway that regulates cell migration and differentiation in early Drosophila mesodermal tissues. 相似文献