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Guohai Wu Ronald W. Wilen Albert J. Robertson Lawrence V. Gusta 《Plant physiology》1999,120(2):513-520
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We investigated the effect of salt stress on enzymatic activity of superoxide dismutase (SOD) isozymes in shoot and root tissues
of salt tolerant and sensitive wheat (Triticum aestivum L. and Triticum durum Defs.) cultivars. Ten day old seedlings were subjected to 0.7 M NaCl stress for 3 and 5 days. Seedlings treated in the same
manner without salt stress served as controls. Activity of SOD isozymes in root and shoot extracts was determined by activity
staining of native polyacrylamide gels. In both shoot and root extracts of examined cultivars two isozymes of SOD, namely
MnSOD and Cu/ZnSOD were identified. Cu/ZnSOD activity comprised 90 % of total SOD activity in both root and shoot tissues.
Salt stress caused 1–1.5 fold increase in MnSOD activity of shoots in tolerant cultivars when compared with non-stressed controls.
Under stress conditions, compared to controls all cultivars exhibited reduced MnSOD activity in root tissues. Cu/ZnSOD activity,
on the other hand, was remarkably enhanced (3–4 fold) in root extracts of the tolerant cultivars, whereas it was reduced in
the sensitive ones. 相似文献
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Igasaki T Akashi N Ujino-Ihara T Matsubayashi Y Sakagami Y Shinohara K 《Plant & cell physiology》2003,44(12):1412-1416
Phytosulfokine (PSK), which has been identified as a plant growth factor, had a dramatic stimulatory effect on the formation of somatic embryos of sugi (Cryptomeria japonica) in the presence of polyethylene glycol. The resultant somatic embryos germinated with synchronous sprouting of cotyledons, hypocotyls and roots, and most of the seedlings grew normally. A cDNA clone for the precursor to the PSK peptide of C. japonica was identified in an expressed sequence tags database. Our results support the existence of a PSK signaling pathway in C. japonica. 相似文献
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