共查询到20条相似文献,搜索用时 31 毫秒
1.
Summary The filum terminale, or terminal portion of the spinal cord, was studied in normal adult frogs (Rana pipiens) by means of light and electron microscopy. Astroglial cells are the predominant elements in this region. The rostral portion of the filum terminale consists mainly of (1) a peripheral dense ring of myelinated and some unmyelinated nerve fibers, and processes of astrocytes terminating at the subpial space; (2) an intermediate zone, in which astrocytes are the main cellular elements in addition to a few degenerated neurons; and (3) a central region where the central canal is lined by dark and light ependymal cells. In the caudal portion of the filum terminale, the amount of neuropil is greatly reduced. This region is formed mainly by astrocytic glial cells and very few neuronal elements. The central canal in the caudal portion is located ventrally and contains a lining consisting almost exclusively of dark ependymal cells. 相似文献
2.
Kazuyori Yamada 《Cell and tissue research》1967,84(4):463-472
Summary Electron microscopic observations have been made of the two epithelial cell types, light barrel-shaped and dark rod-shaped cells in the gall bladder of the mouse.The light cells have a voluminous cytoplasm of low electron opacity in which cell organelles such as mitochondria, elements of granular endoplasmic reticulum, and free ribosomes undergo more or less degenerative changes. However, there are a relatively abundant Golgi apparatus and numerous lysosomal dense bodies. The ultrastructural features of the light cells suggest that they are an aged, degenerative cell type with declining functional activity and a high degree of hydration.The dark cells are characterized by a high concentration of mitochondria and free ribosomes, more or less distinctive elements of granular endoplasmic reticulum, and well developed components of the Golgi apparatus. Such ultrastructural characteristics indicate that the dark cell type has a high synthetic activity.What has been observed in the present study can well be correlated with the results of previous studies on the same cells by methods of light microscopic histochemistry. 相似文献
3.
Summary
Amaranthus plants infected with a virus of rod-shaped particles showed under the light microscope intracytoplasmic amorphous and crystalline inclusions.The submicroscopic organization of mesophyll cells from infectedAmaranthus leaves by electron microscopy is described. Besides big crystalline inclusions, long dark inclusions correspondent to needle-like inclusions observed by light microscopy are definable in the cytoplasm. The amorphous inclusion bodies were formed by an overgrown protrusion of vacuolate cytoplasm containing virus particles, long very dark stained inclusions forming dense bands and rings, normal elements of the cytoplasm such as mitochondria, endoplasmic reticulum and ribosomes, and some spherosomes. Inclusions and virus particles were not found in chloroplasts, mitochondria or nuclei of infected cells. 相似文献
4.
The midgut epithelium of larval and early postlarval brown shrimp has been studied with light and electron microscopy. Ultrastructurally the features of the midgut do not change during these stages of development. On the basis of electron density, two epithelial cell types can be distinguished, and these are referred to as light and dark cells. The dark cells contain more rough endoplasmic reticulum and more free ribosomes than the light cells. Mitochondria in the dark cells have a matrix which is less electron dense than the mitochondrial matrix of the light cells. Both cell types have a microvillous border with a surface coat. The microvilli lack microfilaments within their core, and a terminal web is not differentiated in the stages examined. Tubular smooth endoplasmic reticulum is abundant in the basal portions of the cells. Electron dense, membrane bound vesicles are consistently seen in association with the Golgi apparatus, apical cell surface, and gut lumen and therefore are believed to be secretory granules. Cells in the anterior portion of the midgut often contain very large lipid droplets in the cytoplasm. 相似文献
5.
In vertebrate rod photoreceptor cells, arrestin and the visual G-protein transducin move between the inner segment and outer segment in response to changes in light. This stimulus dependent translocation of signalling molecules is assumed to participate in long term light adaptation of photoreceptors. So far the cellular basis for the transport mechanisms underlying these intracellular movements remains largely elusive. Here we investigated the dependency of these movements on actin filaments and the microtubule cytoskeleton of photoreceptor cells. Co-cultures of mouse retina and retinal pigment epithelium were incubated with drugs stabilizing and destabilizing the cytoskeleton. The actin and microtubule cytoskeleton and the light dependent distribution of signaling molecules were subsequently analyzed by light and electron microscopy. The application of cytoskeletal drugs differentially affected the cytoskeleton in photoreceptor compartments. During dark adaptation the depolymerization of microtubules as well as actin filaments disrupted the translocation of arrestin and transducin in rod photoreceptor cells. During light adaptation only the delivery of arrestin within the outer segment was impaired after destabilization of microtubules. Movements of transducin and arrestin required intact cytoskeletal elements in dark adapting cells. However, diffusion might be sufficient for the fast molecular movements observed as cells adapt to light. These findings indicate that different molecular translocation mechanisms are responsible for the dark and light associated translocations of arrestin and transducin in rod photoreceptor cells. 相似文献
6.
Dr. Raymond Coleman 《Cell and tissue research》1975,164(2):215-232
A comparative optical microscopic and ultrastructural study on the ultimobranchial (UB) glands of three common species of Israeli anurans: Bufo viridis, Hyla arborea, and Rana ridibunda during metamorphosis is presented. The UB glands typically consist of a single follicle with a central lumen, though occasionally secondary follicles are present in Hyla and Rana. A single UB cell type is found which appears either in a very electron-dense "dark" form or as a less dense "light" form, though the ratio of dark: light cells from gland to gland at any one stage of metamorphic development is quite variable. By the end of metamorphosis in Bufo and Hyla all the UB cells are usually of the light variety, whereas in Rana the dark cells persist. The organelles of these secretory cells including secretory granules, granular endoplasmic reticulum, free ribosomes, tonofilaments, microtubules, Golgi bodies, and lipid droplets, their distribution, abundance, and possible functions in relation to metamorphosis are described. Apocrine secretion into the central lumen of the gland is also described and discussed. 相似文献
7.
8.
R.Y. Zacharuk 《Journal of invertebrate pathology》1974,23(1):13-21
Toxins produced by the fungus Metarrhizium anisopliae and the bacterium Pseudomonas aeruginosa in the ecdysial space of a molting wireworm are absorbed through the thin new cuticle and ultrastructurally change the epidermal cells into two distinct types. One is a rounded, degenerative type characterized by a “light” cytoplasm with vesiculated rough endoplasmic reticulum, rounded mitochondria with degenerated cristae, little ground plasm, and a rounded nucleus with little nucleoplasm and large globules of condensed chromatin from which chromatin fibrils separate in loose folds or granulelike tight folds. The other type has very irregular outlines and is characterized by a “dark” cytoplasm with abundant, whorled laminae of rough endoplasmic reticulum and abundant free ribosomes in a dense ground plasm, large rounded clear vacuoles, and apparently normal mitochondria and nuclei. The fungal toxins are believed to be primarily responsible for the formation of the light cells, and the bacterial toxins, for the separation of the chromatin into fibrils in the light cells, the fusion of their nuclei into large nuclear bodies, and the changes in the cytoplasmic contents of the dark cells. The dark cells, although abnormal, appear to retain a limited secretory activity. 相似文献
9.
Identification of two types of melanocyte within the stria vascularis of the mouse inner ear 总被引:4,自引:0,他引:4
J Cable K P Steel 《Pigment cell research / sponsored by the European Society for Pigment Cell Research and the International Pigment Cell Society》1991,4(2):87-101
We have distinguished two types of melanocyte within the intermediate layer of the stria vascularis in the cochlea of normally pigmented mice: light and dark intermediate cells. The light intermediate cells are present in the stria from birth and have the typical appearance of a melanocyte. They are large and dendritic with electron-lucent cytoplasm containing numerous vesicles that show tyrosinase activity, and pigment granules in various stages of development. These granules have the ultrastructural and histochemical characteristics of premelanosomes and melanosomes. The light intermediate cells persist throughout life, but less frequently contain pigment in older animals. The dark intermediate cells, present only in adult mice, vary considerably in number and distribution between animals. Pigment granules, bound within an electron-dense acid phosphatase-rich matrix, form the main component of the dark intermediate cells. The intermediate cells may comprise either two distinct cell populations or different developmental stages of the same cell type; ultrastructural observations suggest the latter. In young mice, light intermediate cells contain the electron-dense matrices, which at later stages of development are found almost exclusively in dark cells. The dark intermediate cells contain few cell organelles other than pigment granules accumulated within lysosomal bodies and they often have pycnotic nuclei. These observations suggest that the dark intermediate cells are a degenerate form of the light intermediate cells. Clusters of melanosomes also occur in the basal cells, and to a much lesser extent in the marginal cells. These cells do not stain after incubation in DOPA, suggesting that they are not capable of melanin synthesis, and therefore probably acquire melanin by donation from adjacent melanocytes. Pigment clusters are also found within the spiral ligament at all stages of development. 相似文献
10.
Summary The processes of horizontal cells invaginating teleost cone pedicles are studded with small finger-like projections which are present only in the light-adapted state. The aim of this study was to investigate whether the formation and degradation of these so-called spinules, which are thought to function as feed-back synapses onto the cones, is endogenously controlled.Three types of experiment were carried out involving fish entrained to a 12 h light/dark cycle: 1) The number of spinules was determined in goldfish at various times during exposure to either constant darkness (36 h) or constant light (57 h). 2) The time course of spinule formation and degradation in goldfish was investigated following exposure to light or darkness at various times during the light/dark cycle. 3) The time course of flash-induced spinule formation in tench following dark adaptation at noon was compared to that following dark adaptation at midnight.The results of these experiments show that spinule formation and degradation are partially under endogenous control but that they need light for full expression. This endogenous rhythm is reflected in the time courses of spinule formation and breakdown during different phases of the light/dark cycle. 相似文献
11.
Light-dependent dynamics of gap junctions between horizontal cells in the retina of the crucian carp
Summary The dynamics of gap junctions between outer horizontal cells or their axon terminals in the retina of the crucian carp were investigated during light and dark adaptation by use of ultrathin-section and freeze-fracture electron microscopy. Light adaptation was induced by red light, while dark adaptation took place under ambient dark conditions. The two principal findings were: (1) The density of connexons within an observed gap junction is high in dark-adapted retina, and low in light-adapted retina. This, respectively, may reflect the coupled and uncoupled state of the gap junction. (2) The size of individual gap junctions is larger in light-than in dark-adapted retinae. Whereas the overall area occupied by gap junctions is reduced with dark adaptation, the percentage of small and very small gap junctions increases dramatically. A lateral shift of connexons in the gap junctional membrane is strongly suggested by these reversible processes of densification and dispersion. Two additional possibilities of gap junction modulation are discussed: (1) the de novo formation of very small gap junctions outside the large ones in the first few minutes of dark adaptation, and (2) the rearrangement of a portion of the very large gap junctions. The idea that the cytoskeleton is involved in such modulatory processes is corroborated by thin-section observations.Dedicated to Professor J. Peiffer on the occasion of his 65th birthday 相似文献
12.
The structural-functional characteristics of the cells of wild type CC-124 and Brc-1 mutant of the unicellular green algae Chlamydomonas reinhardtii grown in the dark and in the light were studied. The cells of the wild type in heterotrophic and mixotrophic conditions had a well developed structure and high functional activity due to the ability of the cells to synthesize chlorophyll both in the light and in the dark. The cells of Brc-1 mutant lost their ability to synthesize chlorophyll in the dark and the cell color was orange due to brc-1 mutation in the nuclear gene LTS3 that regulated the activity of Mg-chelatase enzyme. In the dark the mutant cells accumulated protoporphyrin IX and had weakly developed structure with low functional activity. Because of the high content of protoporphyrin IX, even a short-term exposure of the Brc-1 mutant cells to the light was accompanied by very strong destructive changes in all the membranes in a cell: plasmalemma, chloroplast, mitochondrion, envelopes of the nucleus and vacuoles. The causes of significant impairment of the membrane components and O2-gas exchange in the Brc-1 mutant cells are discussed. 相似文献
13.
The parotid gland of Dasyuroides byrnei was examined by light microscopy, and transmission and scanning electron microscopy. The acini were composed predominantly of seromucous cells with a few mucous cells. The seromucous cells were light or dark cells containing acidophilic spherical granules of moderate to high electron density and had well-developed cytoplasmic organelles-ordinary mitochondria and large mitochondria with tubular cristae, RER with vesicular or tubular elements, and Golgi apparatus with lamellae, vesicles and vacuoles. The mucous cells had basophilic amorphous granules of low electron density, like those of ordinary mucous cells. The intercalated ducts were composed of simple cuboidal light cells having a few electron-dense granules. The striated ducts consisted of tall columnar light cells containing numerous vesicles and mitochondria with tubular cristae, the same as found in acinar seromucous cells. 相似文献
14.
O T Ganeshina 《Zhurnal evoliutsionno? biokhimii i fiziologii》1985,21(3):299-302
Ultrastructural studies have been made on the dark and light cells, as well as on their interconnections with capillaries in the tegmentum vasculosum of the chick cochlea. The dark cells exhibit ultrastructural peculiarities which are typical for ion transporting cells: their lower part is presented by a system of numerous cytoplasmic projections filled with mitochondria. The role of the light cells is less evident. It is suggested that they may control the exchange of ions and other metabolites between the dark cells and capillaries. Significant ultrastructural differences are described between the tegmentum vasculosum in chicks and the stria vascularis in mammals. 相似文献
15.
The distributions and relative frequencies of insulin-, glucagon- and somatostatin-immunoreactive cells were studied in dorsal, ventral, third and splenic lobes of developing chicken pancreas during embryonic periods (10 days of incubation to hatching) by immunohistochemical methods. The regions of pancreas were subdivided into three regions: exocrine, light and dark islet. Round, oval and spherical shaped immunoreactive cells were detected in all four lobes. According to developmental stages, the types of lobes and the regions of pancreas showed various distributions and relative frequencies. In the splenic lobes, insulin, glucagon and somatostatin-immunoreactive cells were detected in exocrine, dark islet and light islet from time differentiation of splenic lobes, 13 days of incubation. The insulin- and somatostatin-immunoreactive cells of the third lobes were detected in exocrine and light islets from 10 days of incubation, and in dark islets from 15 and 11 days of incubation respectively. Glucagon-immunoreactive cells were detected in exocrine, dark and light islets from 16, 11 and 19 days of incubation respectively. These immunoreactive cells of the ventral lobes were detected in exocrine and light islets. However, dark islets were not found in this lobe. Insulin-immunoreactive cells were demonstrated from 10 days of incubation in these two regions. Glucagon-immunoreactive cells were detected from 17 days of incubation in exocrine and 16 days of incubation in the light islets. Somatostatin-immunoreactive cells were demonstrated from 11 days of incubation in exocrine and 14 days of incubation in the light islets. In the dorsal lobes, insulin-immunoreactive cells were demonstrated in exocrine, dark and light islets from 12, 14, and 13 days of incubation, respectively. Glucagon- and somatostatin-immunoreactive cells were detected in dark and light islets from 13 and 14 days of incubation, respectively. Glucagon- and somatostatin-immunoreactive cells were demonstrated from 10 and 11 days of incubation in exocrine respectively. Generally, insulin-immunoreactive cells were increased in light islets but decreased in light islets with developmental stages. However, glucagon-immunoreactive cells were decreased in light islets but increased in dark islets. In addition, somatostatin-immunoreactive cells showed the same frequencies in light and dark islets with developmental stages except exocrine which increased with developmental stages. 相似文献
16.
FZ Djazouli Alim MJ Rodríguez C Andrade N Lebaili N Mahy 《Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society》2012,50(2):203-212
The aim of this work is to study the characteristics of the dromedary nervous lobe and determine how the seasons condition its organization. To this end, electron microscopy was performed and examined quantitatively on animals from winter and summer periods. The results show a higher number of cells in the nervous lobe in summer than in winter. The most abundant glial elements in winter are light pituicytes engulfing neurosecretory nerve fibers making neuroglial contact, and dark pituicytes containing numerous heterogeneous light bodies. In summer, the most distinctive glial cells may be pituicytes in a phagocytic state making contact with characteristic large light bodies that could represent a degenerative process of large neuropeptide storage. Granular pituicytes were also observed in contact with glial and neuronal components. However, lipid droplets, described in pituicytes of other mammals, were not observed in our samples. Quantitative analysis of neurovascular contacts revealed that the number of nerve terminals contacting the basal lamina did not differ between summer and winter, but the mean number of glial processes increased in winter. Our data provides evidence that the storage of neuropeptides is very marked in summer and that, associated with an autophagic and phagocytic phenomenon, this suggests an adaptation to anticipate any situation that would cause dehydration of the dromedary. Thus, in its tough environment, the animal remains permanently prepared to avoid any large water loss. 相似文献
17.
An anatomical analysis of the chicken trigeminal ganglion was made using light microscopy on specimens prepared by usual chemical fixation or freeze-drying methods and by electron microscopy. Two types of neurons were consistently seen, dark and light cells. Dark cells contained a dense cytoplasm with Nissl substance distributed evenly throughout, whereas light cells had a less dense cytoplasm containing clumps of Nissl substance. The Nissl bodies in light cells contained only a few small cisternae of granular endoplasmic reticulum as compared with many stacked cisternae in Nissl bodies of dark cells. The ratio of dark to light cells was approximately 62:38 in all regions of the ganglion. Dark cells were consistently smaller than light cells. In the seven-day old chick, the mean diameters of the dark and light neurons were 21.4 μ and 29.5 μ respectively; in the adult the values were 29.9 μ and 39.7 μ respectively. It is concluded that the dark and light cells belong to two distinct neuronal cell populations. 相似文献
18.
Pathway of dark inorganic carbon fixation in two species of diatoms: influence of light regime and regulator factors on diel variations 总被引:2,自引:0,他引:2
Planktonic algae submitted to vertical mixing with a short periodicitycommute many times a day from low to high irradiance levels.To study the influence of this light periodicity, two diatoms,Skeletonema coslatum and Nitzschia turgiduloides, were cultivatedunder alternating conditions of 2 h light/2 h dark (2 h/2 h),simulating vertical mixing in the natural environment. Two otherlight regimes were used: continuous light (CL) and alternatecycles of 12 h light/12 h dark (12 h/12 h). Products synthesizedin the dark by S.costmum during 60 s incubation for 2 h/2 hculture or during 5 min for 12 h/12 h culture were determined.They were essentially sugars, malate, aspartate and glyceratefor 2 h/2 h cells and 12 h/12 h cells taken at the beginningof the light period. In contrast, 12 h/12 h cells taken duringthe darkness or in the middle of the light period and set inthe dark synthesized only amino acids. Our results corroborateprevious reports on dark CO2 fixation via phosphoenolpyruvatecarboxykinase (PEPCKase, enzyme allowing the fixation of CO2on PEP and the synthesis of amino acids) with involvement ofa substrate synthesized during the light period, but demonstratethat incorporation also occurs by the C-3 pathway (pathway responsiblefor the major CO2 fixation in the light) in the very early stagesof the dark period. Another important result highlighted bythis study is the appreciable rate of dark fixation: on average6.7, 8.3 and 12.7% of photosynthesis at saturating photon fluxdensity for N.turgiduloides cultivated under 2 h/2 h, CL and12 h/12 h regime respectively and nearly 12% for S.costatumin the 2 h/2 h light regime. Variation of dark fixation wasinvestigated as a function of hour in the two species. Skeletonemacostatum cells submitted to the 2 h/2 h cycle show a constantrate of light-independent assimilation throughout the day. Bycontrast, both N.turgiduloides grown under the 12 h/12 h or2 h/2 h regime and S.costatum cultured under the 12 h/12 h cycleundergo fluctuations in the rate of dark fixation over the light/darkcycle. The mean dark fixation rate is controlled by the lengthof the photoperiod or the frequency of light fluctuations, dependingon species. We argue that this phenomenon must be taken intoconsideration in primary production calculations. Dependingon whether they are synthesized at the beginning or at the endof the light period, products are somewhat different and therate of fixation varies. This leads us to suggest that the pathwayof dark fixation may be regulated by at least two factors: amountof available substrate and enzyme (RuBPCase and PEPCKase) activityand/or amount. 相似文献
19.
Potassium ions are a prerequisite for the development and regulation of sensory cell stimulation in the inner ear. From the potassium-rich endolymph the ions flow into the sensory cells apically and are released basolaterally. After transport pathways of various lengths potassium is released again into the endolymph - in the cochlea by marginal cells of the stria vascularis, in the vestibular labyrinth by dark cells. While this long recycling pathway is relatively well-known in the cochlea, few studies have been conducted on the semicircular canal ampullae (SCCA) where its morphological basis is largely unknown. According to the present electron microscopic findings, potassium ions are initially released into the extracellular space during stimulation of the sensory cells and then absorbed by supporting and light cells. Finally they are transported transcellularly over numerous very long gap junctions into the region of the dark cells. From here they move to an extracellular compartment, which is more or less completely sealed off basally by basal plates of the light cells. Apically the intercellular space between light and dark cells is sealed by junctional complexes. This newly identified space in the SCCA corresponds to the extracellular compartment between the marginal and intermediate cells in the stria vascularis. At both sites, the cochlea and the SCCA, this probably serves as a regulatory valve, reservoir or storage space, particularly for potassium ions. It is likely that the different morphology of the ion transport pathways is related to the different flow levels of potassium ions expressed by the different levels of the so-called endocochlear potential and concomitant movement of other ions in the cochlea and SCCA. 相似文献
20.
Mildred Behrens Wolf Krebs 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1976,107(1):77-96
Summary The fine structure of retinular cells within lateral eyes ofLimulus polyphemus which had been dark or light adapted for 12 h in vivo was studied via electron microscopy. The ommatidium to ommatidium and retinular cell to retinular cell variability observed in light microscope studies was confirmed. The rhabdomeric microvilli were longer and narrower, the area of contiguous microvillar membranes greater, the endoplasmic reticulum less abundant and the mitochondrial granules (? calcium containing) more numerous in well dark adapted than in well light adapted retinular cells (Figs. 1, 3, 4, 7, 8) and membrane whorls or vacuoles were present in the peripheral cytoplasm of very well light adapted retinular cells (Fig. 6). Phagocytotic vesicles, multivesicular bodies and lysosomes were present in the interrhabdomeral cytoplasm of partially light adapted retinular cells (Figs. 1, 2, 3, 10). The number of retinular cell microvilli in contact with the eccentric cell dendrite was smaller in very well light adapted than in well dark adapted ommatidia (Fig. 9). The possible functional significance of these light-dependent structural changes is discussed.This investigation was supported in part by Grant 2 RO1 EY 00236 National Eye Institute, National Institutes of HealthMember of the SFB 160 of the Deutsche Forschungsgemeinschaft 相似文献