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A relationship between the estrous cycle and non-specific host resistance to Paracoccidioides brasiliensis yeast cells was examined by using both sexes of adult BALB/c mice. They were divided into 6 groups, including a male group and females at proestrus, estrus, metestrus-I, metestrus-II and diestrus. The mice received yeast cells through three different inoculation routes; intravenous, intraperitoneal and intratracheal. In all of the inoculation routes, the clearance of the yeast cells was influenced by the estrous cycle. The female mice at estrus, which might have high blood estrogen levels, showed a marked clearance of the yeast cells from the blood, peritoneal cavity and lungs. These results suggested that non-specific host resistance to the yeast cells was enhanced by estrogen. All female groups inoculated by the three routes showed higher clearance of the yeast cells than the male group. 相似文献
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Christina Andersson Dunstan Jan Andersson Lars Bohlin Paul A. Cox Kjell-Olov Grönvik 《Cytotechnology》1994,14(1):27-38
A water soluble extract from the bark of the Samoan medicinal plantAlphitonia zizyphoides A. Gray (Rhamnaceae), enhances the plating efficiencyin vitro of lymphoid cell lines as well as the survival of bone marrow cells and normal T and B lymphocytes. Furthermore, the inclusion of bark-extract into culture media enhances the cloning efficiency of a T-hybridoma cell line by more than 30 times at otherwise unsuitably low serum concentrations, but does not completely substitute for serum. The enhanced growth of a B-cell hybridoma is also paralleled by an increased production of monoclonal antibodies in cultures containing low cell densities.Abbreviations AZH/w
water extract ofAlphitonia zizyphoides
- AZH/c
sugar-free fraction of the same extract
- CM
complete medium
- FCS
fetal calf serum
- NMR
nuclear magnetic resonance
- PBS
phosphate buffered saline
- SEM
standard error of the mean
- TLC
thin layer chromatography 相似文献