共查询到10条相似文献,搜索用时 31 毫秒
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J I Peterson 《Analytical biochemistry》1968,25(1):257-259
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Ann M. Jose 《Planta》1977,134(3):287-293
Pelletable phytochrome from hypocotyl hooks of Cucurbita pepo L. seedlings has been separated into two fractions by gel filtration on Sepharose CL-2B. One fraction with a K
av of 0.7 was detected only after red irradiation (in vivo or in vitro). This separated from a ribonucleoprotein fraction during gel filtration. The weak interaction with ribonucleo-protein which required magnesium (optimal at 10 mM) was overcome by high salt concentrations and prevented by ribonuclease treatment. The second phytochrome fraction was strongly associated with a high molecular weight material with a K
av of less than 0.1. Low levels of this complex were detected in extracts from dark grown tissue but were increased by red irradiation of excised hooks or crude extracts. The binding of phytochrome to the high molecular weight material did not require magnesium, was unaffected by ribonuclease treatment, and was much more resistant to high salt concentrations than was the phytochrome—ribonucleoprotein association. These results suggest that the association of phytochrome with this membrane—containing fraction is not electrostatic.The separation by agarose-gel filtration offers a useful technique for the preparation of membraneassociated phytochrome for physiological studies.Abbreviations EDTA
ethylenediaminetetraacetic acid
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P
r and Pfr
phytochrome in the red and far-red absorbing forms
- RNase
ribonuclease
- RNP
ribonucleoprotein 相似文献
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J A Alhadeff 《The Biochemical journal》1978,173(1):315-319
The role of sialic acid in the gel-filtration behaviour of sialoglycoproteins was investigated by using the separated isoenzymes of purified human liver alpha-L-fucosidase and several other well-known sialic acid-containing glycoproteins (fetuin, alpha1-acid glycoprotein, thyroglobulin and bovine submaxillary mucin). For each glycoprotein studied, gel filtration of its desialylated derivative gave an apparent molecular weights much less than that expected just from removal of sialic acid. For the lower-molecular-weight glycoproteins (fetuin and alpha1-acid glyocprotein), gel filtration of the sialylated molecules led to apparent molecular weights much larger than the known values. The data indicate that gel filtration cannot be used for accurately determining the molecular weights of at least some sialoglycoproteins. 相似文献
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The behavior of a number of acylated deoxyribooligonucleotides in gel permeation chromatography has been studied and dependence on factors other than molecular size has been noted. Retardation has been observed to increase as follows: pT < d-pABz < d-pCAn < d-pGiBu, and this order is reflected in the elution parameters of derived oligomers. Certain nucleotide derivatives—notably d-pCAn and its relatives—were eluted in two peaks. 相似文献
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O. Smithies 《Genetics》1995,139(1):1-4