共查询到20条相似文献,搜索用时 15 毫秒
1.
Javier Turnay Nieves Olmo José G. Gavilanes Javier Benitez Maria A. Lizarbe 《Cytotechnology》1990,3(1):75-88
A new cell line designated as BCS-TC2 was established in culture from a primary human colon adenocarcinoma. This cell line has been in continuous culture over a 36-month period. The cells grow as a monolayer sheet, displaying areas with a multilayered pattern as well as single cells and free-floating aggregates. The morphological, immunological, and ultrastructural features of these cells are in agreement with their epithelial origin. The characterization of this cell line indicated a 38 hr doubling time, and a colony forming efficiency of 2% in semisolid media and 22% in liquid culture, at low cell densities. These cells produce low amounts of carcinoembryonic antigen in culture (0.1 ng of CEA/106 cells). Sub-cutaneous injection into athymic mice shows that these cells have a non-tumorigenic capacity. Chromosomal analysis showed a karyotype 46 XX,-15, +der (15), inv (16) (p13::q13). BCS-TC2 cell line, which maintains in culture several characteristics of the original tumor, represents a useful model system for cell biology studies of primary and non-metastatic tumors. 相似文献
2.
Souei Sekiya Teruyo Kaiho Syoichi Shirotake Hiroshi Iwasawa Noriyuki Inaba Makoto Kawata Koji Higaki Hideo Ishige Hiroyoshi Takamizawa Masako Minamihisamatsu Tsuguo Kuwata 《In vitro cellular & developmental biology. Plant》1983,19(6):489-494
Summary A human nongestational choriocarcinoma cell line of ovarian origin (IMa) was established in vitro. This cell line had been
subcultured serially more than 22 times over 18 months. Small polygonal cells with a prominent nucleus were dominant and a
sparsity of cytoplasmic organelles was an ultrastructural characteristic of the IMa cells. The production and secretion of
human chorionic gonadotropin and its subunits were identified by radioimmunoassay. The IMa cells were transplantable in the
hamster cheek pouch and the histological diagnosis was choriocarcinoma. A newly established ovarian choriocarcinoma cell line
can be considered useful for clarifying the biological differences between nongestational and gestational choriocarcinoma
cells.
This research was supported in part by a Grant-in-Aid for Cancer Research from both the Ministry of Health and Welfare and
the Ministry of Education, Science, and Culture of Japan. 相似文献
3.
R. T. Morgan L. K. Woods G. E. Moore L. McGavran L. A. Quinn T. U. Semple 《In vitro cellular & developmental biology. Plant》1981,17(6):503-510
Summary A continuous cell line, COLO 346, was established from a liver metastasis in a patient with adenocarcinoma of the gallbladder.
COLO 346 grew as an adherent monolayer of pleomorphic epithelioid cells. COLO 346 cells produced esterone, but no estradiol,
progesterone, or cortisol. No adrenocorticotropic hormone, β-subunit of human chorionic gonadotropin, carcinoembryonic antigen,
or α-fetoprotein production by the cells was detected. Cell doubling time was 36 h. Seven allelic isozymes were assayed. COLO
346 had a chromosome mode of 74 at 21 months postestablishment with 6 marker chromosomes present in 100% of the cells analyzed.
COLO 346 has been in continuous culture for over 2 yr and is available to other investigators for their studies.
This paper was presented in part at the 31 st Annual Meeting of the Tissue Culture Association, June 1–5, 1980. The work was
supported by Grants CA15018 and CA29514 from the National Cancer Institute, and by the Mary B. and L. H. Marshall Fund. 相似文献
4.
Human colonic adenocarcinoma cells 总被引:5,自引:0,他引:5
Baldwin H. Tom Lynne P. Rutzky Milda M. Jakstys Ryoichi Oyasu Celia I. Kaye Barry D. Kahan 《In vitro cellular & developmental biology. Plant》1976,12(3):180-191
Summary A series of human colonic epithelial cell lines have been cultured from a single patient: LS-180 the original adenocarcinoma,
LS-174T a trypsinized variant, and normal colonic tissue. The malignant cells, 20 to 40 μm in diameter and oval to polygonal,
exhibited characteristics of normal colonic mucosal cells, namely, abundant microvilli prominent in secretory cells, and the
presence of intracytoplasmic mucin vacuoles. The cultured adenocarcinoma cells, but not normal, demonstrated neoplastic properties
by producing high levels of carcinoembryonic antigen (CEA) and by the ability to be propagated in hamster cheek pouches and
in immunodeprived mice. The CEA production by the newly established line LS-180 released 900 times more CEA per cell into
the culture medium and bore 30 times more cell-associated material than the established line, HT-29. These cell lines may
permit detection of distinctive chemical, physiological, pharmacologic, and immunologic characteristics of neoplastic colonic
cells.
The use of the term “line” conforms with the recent definitions published by Fedoroff in theTissue Culture Association Manual, Vol. 1, No. 1, pp. 53–57, 1975. 相似文献
5.
研究东亚钳蝎毒素对人结肠癌细胞Caco-2增殖的影响。以不同浓度的东亚钳蝎(Buthus martensii Karsch)毒素(10、20、40滋g/mL)干预体外培养的Caco-2细胞,分别于24 h、48 h后,用四甲基偶氮唑盐(MTT)比色法,观察毒素对Caco-2细胞的增殖抑制作用。运用淋巴细胞转化实验和乳酸脱氢酶(LDH)释放实验检测蝎毒素对Caco-2细胞的作用途径。结果表明:东亚钳蝎毒不仅能抑制Caco-2细胞的增殖而且能促进淋巴细胞转化,毒素对Caco-2细胞增殖的抑制作用与浓度和作用时间密切相关。 相似文献
6.
Characterization of WiDr: A human colon carcinoma cell line 总被引:1,自引:0,他引:1
P. Noguchi R. Wallace J. Johnson E. M. Earley S. O'Brien S. Ferrone M. A. Pellegrino J. Milstien C. Needy W. Browne J. Petricciani 《In vitro cellular & developmental biology. Plant》1979,15(6):401-408
Summary We describe the establishment and characterization of WiDr, a cell line derived from a human colon carcinoma. It produces
carcinoembryonic antigen in culture, and has a doubling time of 15 hr with plating efficiency of 51%. The HLA antigenic profile
and the allozyme genetic signature (composed of eight gene-enzyme systems) of WiDr cells are different from those of HeLa
cells. Furthermore, WiDr cells possess three marker chromosomes, again distinct from the HeLa marker chromosomes. Finally,
it is highly tumorigenic in four different xenogeneic animal models. Based on these studies, WiDr represents a useful model
cell line for tumor cell biology investigations. 相似文献
7.
Terry B. White Dianne K. Hammond Hernán Vásquez Henry W. Strobel 《Molecular and cellular biochemistry》1991,102(1):61-69
Cytochrome P450 is known to cause carcinogen activation and correspondingly increased cancer risk in animal models. In order to determine whether P450 in the colon may be involved in cancer development in the human, the human colon cell line LS174T was examined for the presence of various cytochromes P450. Two isozymes of P450 were identified in the human cell line. Expression of P450IAl or IA2 was increased by treatment of the cell line with benzanthracene; the induction was demonstrated by an increase in RNA hybridizing to a probe for P4501Al and by ethoxyresorufin deethylation activity. Western analysis of microsomes isolated from human colon tissue also demonstrated the presence of P4501A1, as well as a form which cross-reacted to an antibody to human P450IIC9. Another isozyme, P450IIE1, was identified by polymerase chain reaction amplification of RNA from LS174T cells. These results underscore the presence of cytochromes P450 in colonic tissue and provide a basis for the involvement of isozyme-specific P450 mediated reactions in carcinogenesis of the colon.Some of the data presented here were taken from a thesis submitted by D.K.H. in partial fulfillment of the requirements for the Ph.D. degree in the University of Texas Graduate School of Biomedical Sciences. 相似文献
8.
Leonard A. Cohen 《In vitro cellular & developmental biology. Plant》1982,18(6):565-575
Summary A new in vitro model for human breast cancer is described. Derived from anN-nitrosomethylurea (NMU) induced rat mammary adenocarcinoma, this serially cultivated cell line has been demonstrated, by
a variety of criteria, to be an authentic neoplastic, rat mammary epithelial cell line. The criteria used include morphological
and growth characteristics; the presence of specific cell surface antigens; steroid hormone receptors; hormone responsiveness;
casein production; karyotype and isoenzyme profile analysis; anchorage independent growth and oncogenicity. Inasmuch as the
NMU cell line possesses high concentrations of glucocorticoid and androgen receptors, it may provide a useful model for study
of the action of these hormones in human breast cancer. In addition, the NMU line may serve as a valuable in vitro model in
which to assess the effects of a variety of endogenous and exogenous agents known to influence mammary tumor growth in vivo,
including drugs, nutrients, and growth factors.
This work was supported by Grants CA29602 and RR05775-05 from the National Cancer Institute, Bethesda, Maryland. 相似文献
9.
Characterization of a cell line (SW756) derived from a human squamous carcinoma of the uterine cervix 总被引:6,自引:0,他引:6
Ralph S. Freedman James M. Bowen Albert Leibovitz Sen Pathak Michael J. Siciliano Harry S. Gallager Beppino C. Giovanella 《In vitro cellular & developmental biology. Plant》1982,18(8):719-726
Summary An established cell line, SW756, derived from a primary squamous carcinoma of the uterine cervix is described by its morphology,
ultrastructure, karyotype, genetic signature analysis, HLA typing, and tumorigenesis in the nude mouse. Cultured cells obtained
from the SW756 derived nude mouse tumor also were studied for chromosome and isozyme markers. The original tumor was poorly
differentiated carcinoma with minimal keratinization and is compared with that occurring in the nude mouse after the cultured
cells were inoculated. The nude mouse tumor showed similar histological features, but better differentiation than the original
tumor. Karyotype analysis of SW756 demonstrated a hyperdiploid stem line number and several marker chromosomes (MI-M6). No
HeLa marker chromosomes were identified. The isozyme pattern for SW756 reported by others has been confirmed. The unique chromosome
and isozyme features have been identified repeatedly in the cultured cells and, most importantly, in the post nude mouse culture.
We recommend SW756 as a defined human tumorigenic cell line derived from a primary squamous carcinoma of the uterine cervix.
This investigation was supported in part by Public Health Research Grant CA-06294 from the National Cancer Institute, Department
of Health and Human Services. 相似文献
10.
Establishment of a human fetal cardiac myocyte cell line 总被引:4,自引:0,他引:4
Yi-Chong Wang Nicolas Neckelmann Ann Mayne Ahvie Herskowitz Alagarsamy Srinivasan Kenneth W. Sell Aftab Ahmed-Ansari 《In vitro cellular & developmental biology. Animal》1991,27(1):63-74
Summary Human cardiac myocytes undergo degeneration, cytolysis, and necrosis in a number of clinical disease conditions such as myocarditis,
dilated cardiomyopathy, and during episodes of cardiac allograft rejection. The precise cellular, biochemical, and molecular
mechanisms that lead to such abnormalities in myocytes have been difficult to investigate because at present it is not possible
to obtain and maintain viable cell cultures of human adult cardiac myocytes in vitro. However, human fetal cardiac myocytes
are relatively easy to maintain and culture in vitro, but their limited availability and growth, variability from one preparation
to another, and varying degrees of contamination with endothelial and epithelial cell types have made it difficult to obtain
reliable data on the effect of cardiotropic viruses and cardiotoxic drugs on such myocytes. These thoughts prompted us to
attempt to derive a cell line of human cardiac origin. Highly enriched human fetal cardiac myocytes were transfected with
the plasmids pSV2Neo and pRSVTAg and gave rise to a cell line (W1) which has been maintained in culture for 1 yr. Morphologic
and phenotypic analyses of W1 cells by flow microfluorometry and immunoperoxidase techniques indicate that the W1 cell line
shares many properties of human fetal cardiac myocytes, but appears not to react with specific antibodies known to react with
markers unique to human endothelial, epithelial, skeletal muscle, and dendritic cells. These preliminary data suggest that
the W1 cells may provide a unique source of an established cell line that shares many properties ascribed to human cardiac
myocytes.
This study was supported by grant 1RO1-25566-03 from the National Institutes of Health, Bethesda, MD, to A. Ahmed-Ansari and
by a Grant-in-Aid from the American Heart Association, Georgia Affiliate, to Nicolas Neckelmann. 相似文献
11.
A new diploid nontumorigenic human breast epithelial cell line isolated and propagated in chemically defined medium 总被引:10,自引:0,他引:10
P. Briand O. W. Petersen B. Van Deurs 《In vitro cellular & developmental biology. Plant》1987,23(3):181-188
Summary A new, nontumorigenic human breast epithelial cell line, HMT-3522, has been established from fibrocystic breast tissue. Cells
were explanted and propagated in chemically defined medium including insulin, transferrin, epidermal growth factor, hydrocortisone,
estradiol, prolactin, and Na-selenite. The epithelial nature of the cell line was established by immunocytochemical detection
of cytokeratins. Moreover, electronmicroscopy revealed monolayers of polarized cells connected by desmosomes and provided
with apical microvilli. Milk fat globule membrene antigen, specific for the apical membrane domain of normal, luminal breast
epithelial cells, was expressed only in confluent cultures where some cells overlaid others, indicating “stem cell”-like properties.
After 25 to 30 passages, the cells are diploid with a few marker chromosomes and loss of chromosomes in the D-group. The cells
are nontumorigenic in athymic mice; they lack estrogen receptors, and estradiol does not stimulate growth. The HMT-3522 cell
line may represent a useful model for the study of brest cell differentiation and carcinogenesis in vitro.
This work was supported by a grant from the Danish Cancer Society. 相似文献
12.
Summary Primary and passaged cultures of normal colon epithelial cells, derived from human fetuses (13 to 17 wk of conceptual age)
have been established. These cultures have been passaged 16 times thus far. The cultures have been initiated and maintained
in medium consisting of 50% Dulbecco's minimum essential medium and 50% Ham's F12 medium and supplemented with antibiotics
(penicillin, 100 U/ml; streptomycin, 100 μg/ml); ascorbic acid, 40 μg/ml;l-isoleucine, 50 μg/ml; epidermal growth factor, 20 ng/ml; insulin, 5 μg/ml; cholera toxin, 5 ng/ml; transferrin, 1 μg/ml;
fetal bovine serum (10%); and HEPES, 25 mM final concentration, and incubated at 37°C in humidified gas containing 5% CO2: 95% air.
The cellular and subcellular characteristics of primary and passaged cultures were defined using light microscopy and scanning
and transmission electron microscopy. The cells exhibited microvilli on cell surfaces and showed junctional complexes and
interdigitations between cells. Indented nuclei with dense chromatin and marginated heterochromatin, numerous mitochondria,
rough endoplasmic reticulum, polysomes, and extensive Golgi zones were conspicuous. Also, periodic acid Schiff's reagent-positive
staining of the cells suggests the active synthesis of complex mucopolysaccharides in the cytoplasm.
This study was supported by USPHS Grant CA-30185 from the National Large Bowel Cancer Project, National Cancer Institute. 相似文献
13.
Lun He Kurt J. Isselbacher Jack R. Wands Howard M. Goodman Chiaho Shih Andrea Quaroni 《In vitro cellular & developmental biology. Plant》1984,20(6):493-504
Summary A human hepatocellular carcinoma cell line (FOCUS—Friendship of China and United States) was derived from a patient with primary
hepatocellular carcinoma. This cell line has been in continuous culture over an 18-mo period. The morphological and ultrastructural
features of FOCUS are consistent with its neoplastic hepatocellular orgin. FOCUS cells contain aspartate aminotransferase
and glucose-6-phosphatase activity. In addition, α1-antitrypsin, fibrinogen, alpha fetoprotein, and carcinoembryonic antigens were detectble in the cytoplasm of the cultured
cells by immunochemical staining techniques. The karyotype of the FOCUS cell is human in origin and it contains human DNA
sequences as detected by molecular hybridization analysis. The FOCUS cells do not show evidence of density-dependent inhibition
of growth under confluent conditions. Repeated growth curves over an 18-mo period were identical, revealing a doubling time
of 42 to 48 h. The malignant potential of FOCUS cells was further demonstrated by their ability to lead to gross tumor formation
after subcutaneous infection into nude mice. From one of the solid tumors grown in nude mice, recultured cell lines have been
established and found to have properties identical to the original FOCUS cell line. This FOCUS cell line represents an additional
model for further investigation of tumor specific antigens and the relationship between hepatitis B virus (HBV) and hepatocellular
carcinoma. Preliminary molecular characterization has indicated the existence of integrated HBV sequences within the FOCUS
genome. 相似文献
14.
Aoki D Suzuki N Susumu N Noda T Suzuki A Tamada Y Higashiguchi A Oie S Nozawa S 《Human cell》2005,18(3):143-146
A cell line, designated as RMG-V, was established from a patient with clear cell adenocarcinoma of the ovary. The cell line has grown without interruption and has been propagated continuously by serial passaging (more than 36 times) over 5 years. The cells are spindle-shaped, display neoplastic and pleomorphic features, and grow in a jigsaw puzzle-like arrangement while forming monolayers without contact inhibition. These cells proliferate rapidly, and the population doubling time is about 15.5 hours. The number of chromosomes ranges between 77 and 85, with a modal number of 83. 相似文献
15.
Summary Lectins were used to characterize mucin glycoproteins and other secretory glycoconjugates synthesized by a human colon adenocarcinoma-derived cell line which expresses a goblet cell phenotype. Despite being clonally derived, HT29-18N2 (N2) cells, like normal goblet cells in situ were heterogeneous in their glycosylation of mucin. Only wheat-germ agglutinin, which recognizes N-acetylglucosamine and sialic acid residues, and succinylated wheat-germ agglutinin, which binds N-acetylglucosamine, stained the contents of all secretory granules in all N2 goblet cells. The N-acetylgalactosamine binding lectins Dolichos biflorus and Glycine max stained 20% and 21% of N2 goblet cells respectively. Ricinus communis I, a galactose-binding lectin, stained 67% of N2 goblet cells although staining by another galactose-binding lectin, Bandeiraea simplicifolia I, was limited to 19%. Peanut agglutinin, a lectin whose Gal(1–3)GalNAc binding site is not present on mucins produced in the normal colon but which is found on most mucins of cancerous colonic epithelia, stained 68% of the cells. Ulex europeus I, a fucose-binding lectin, did not stain any N2 goblet cells. Four lectins (Lens culinaris, Pisum sativum, Phaseolus vulgaris E, Phaseolus vulgaris L) which recognize sugars normally present only in N-linked oligosaccharides stained up to 38% of N2 goblet cells. The binding of these lectins indicates either both O-linked and N-linked oligosac-charide chains are present on the mucin protein backbone or the co-existence of non-mucin N-linked glycoproteins and O-linked mucins within the goblet cell secretory granule. 相似文献
16.
Kwong-Y. Tsang G. Shashidhar Pai H. Hugh Fudenberg 《In vitro cellular & developmental biology. Plant》1981,17(4):308-314
Summary The characteristics of Cell Line LM-1, established from a human osteosarcoma, have been studied extensively. The cell produced
both bone-specific and placental-like alkaline phosphatases when treated with hydrocortisone 21-phosphate; they had specific
membrane antigens that reacted with sera from osteosarcoma patients. Injection of LM-1 cells into newborn hamsters treated
with antilymphocyte serum produced nodular tumors. The characteristics of LM-1 suggest that this tumor cell line has unique
features that may be useful in a variety of studies of human and animal osteosarcoma.
This research is supported in part by USPHS Grants HD-09938 and CA-25746. 相似文献
17.
18.
Shashi Shrivastav Yousuf Sharief John Day Charles F. Reich Robert A. Bonar 《In vitro cellular & developmental biology. Plant》1981,17(12):1117-1124
Summary A new cell line, SS78, was established from a primary renal cell carcinoma of a Caucasian male. The tissue was dispersed with
collagenase, and viable cells were separated by flotation on a Ficoll-Hypaque gradient. In culture, the SS78 cells retained
a distinct epithelial morphology, and no fibroblastlike cells were seen. The cultured cells were aneuploid with a modal chromosome
number of 80 and had several marker chromosomes. Inoculation of the cultured cells into athymic nude mice caused tumors at
the sites of inoculation.
This research was supported in part by Grants CA 15972 and CA 14930 from the National Cancer Institute through the National
Bladder Cancer Project and by the Medical Research Service of the Veterans Administration. 相似文献
19.
Amylin binding sites in a human hepatoblastoma cell line (HepG2) have been characterized in detail. 125I-Amylin (rat) bound to HepG2 cells with high affinity. Binding was reversible and selective, and dependent on time and temperature. Scatchard analysis revealed the presence of high (Kd = 0.11 ± 0.04 nM) and low (Kd = 1.3 ± 0.4 μM) affinity binding sites for 125I-amylin in HepG2 cells. The dissociation experiments also showed that 125I-amylin dissociated from high- and low-affinity sites. The association data, however, indicated the presence of only one binding site. Rat amylin was more potent than human amylin and rat calcitonin gene-related peptide (CGRP) in displacing 125I-amylin bound to HepG2 cells. Nonhomologous peptides did not displace 125I-amylin. Rat amylin was, however, less potent than rat CGRP in displacing 125I[Tyr0]CGRP from HepG2 cells. Pretreatment of HepG2 cells with rat amylin (10 nM) reduced the specific binding of 125I-amylin by 75%, whereas rat CGRP (10 nM) pretreatment had no effect on amylin binding. Calcitonin gene-related peptide, as well as rat and human amylin, stimulated the adenylate cyclase activity of HepG2 cell membrane preparation in a dose-dependent manner, with an order of potency of CGRP > rat amylin > human amylin. A CGRP antagonist, CGRP(8–37), significantly attenuated the stimulatory effect of both amylin and CGRP on adenylate cyclase activity. These investigations show that distinct receptors of amylin and CGRP are present in HepG2 cells and that amylin stimulates adenylate cyclase activity through CGRP receptors. This system could now be exploited for studying amylin receptors and amylin-mediated signal transduction. 相似文献
20.
Hirohisa Yano Masamichi Kojiro Toshiro Nakashima 《In vitro cellular & developmental biology. Plant》1986,22(11):637-646
Summary A human hepatocellular carcinoma (HCC) cell line (KYN-1) has been established from a resected HCC of a 58-yr-old Japanese,
male patient with HCC. Original resected HCC was moderately differentiated and proliferated in a solid pattern with vague
trabecular structure in part. This cell line has been maintained for 10 mo. through 50 passages. Morphological features of
KYN-1 cells demonstrated one or more large, round-to-oval nuceli with prominent nucleoli and eosinophilic polygonal-to-spindle
abundant cytoplasm. In addition, some of these cells contained mucicarmin-positive materials in the cytoplasm. The cells exhibited
a typical epithelial feature with pavementlike cell arrangement, and lacked contact inhibition. The doubling times of the
cells grown in a serum-containing and a serum-fre, medium were about 31 h and 10 to 11 d, respectively. Functonally, KYN-1
cells produced albumin, α-fetoprotein (AFP), carcinoembryonic antigen (CEA), ferritin, β2-microglobulin (BMG), and α1-anti-trypsin (AAT). Positive reactions for albumin, AFP, CEA, and ferritin were identified in the cells by immunohistochemical
techniques. Chromosome study revealed the chromosome number in a range from 61 to 74 without mode. The tumorigenicity of KYN-1
cells was identified by the tumor formation after subcutaneous inoculation of the cells into nude mice. The developed tumor
showed compact growth of the tumor cells with gland formations containing mucicarmin-positive materials. Features of adenocarcinoma
were identified by electron microscopy. The tumor cells were also identified to contain albumin, AFP, CEA, ferritin, and AAT
by immunohistochemical techniques. AFP, CEA, and BMG were detected in the sera of nude mice. Thus, KYN-1 cells represented
the morphologic features of adenocarcinoma, retaining some characteristics of original HCC. These findings suggest that KYN-1
is a new human HCC cell line with transformation to adenocarcinoma, which will provide useful information to clarify the histogenesis
of combined hepatocellular and cholangiocellular carcinoma.
This study was supported in part by the Sarah Cousins Fund. 相似文献