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1.
K. Richter J. Schondelmaier C. Jung 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(8):1225-1234
Resistance loci for seedling-stage resistance to net blotch disease (Drechslera teres) in barley were mapped with molecular markers in an F2 population derived from a cross between the susceptible barley cultivar ‘Arena’ and the resistant Ethiopian landrace ‘Hor
9088’. Disease reactions were scored with first and second leaves of 2-week-old plants 7 and 9 days after inoculation with
a single spore-derived isolate. For linkage analysis, 22 RFLP markers and 284 AFLP markers were used. The seven linkage groups
covered 1153.3 cM with an average marker interval of 3.76 cM. The resistance was determined to be inherited in a quantitative
manner. Altogether, 12 QTLs were mapped with positions depending on the leaf used for testing and the time period after infection.
Heritability in the broad sense ranged between 0.21 and 0.37.
Received: 26 May 1998 / Accepted: 9 June 1998 相似文献
2.
Identification of quantitative trait loci contributing to Fusarium wilt resistance on an AFLP linkage map of flax (Linum usitatissimum) 总被引:3,自引:0,他引:3
W. Spielmeyer A. G. Green D. Bittisnich N. Mendham E. S. Lagudah 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(4):633-641
An AFLP genetic linkage map of flax (Linum usitatissimum) was used to identify two quantitative trait loci (QTLs) on independent linkage groups with a major effect on resistance
to Fusarium wilt, a serious disease caused by the soil pathogen Fusarium oxysporum (lini). The linkage map was constructed using a mapping population from doubled-haploid (DH) lines. The DH lines were derived
from the haploid component of F2 haploid-diploid twin seed originating from a cross between a polyembryonic, low-linolenic-acid genotype (CRZY8/RA91) and
the Australian cultivar ‘Glenelg’. The AFLP technique was employed to generate 213 marker loci covering approximately 1400 cM
of the flax genome (n=15) with an average spacing of 10 cM and comprising 18 linkage groups. Sixty AFLP markers (28%) deviated
significantly (P<0.05) from the expected segregation ratio. The map incorporated RFLP markers tightly linked to flax rust (Melamspora lini) resistance genes and markers detected by disease resistance gene-like sequences. The study illustrates the potential of
the AFLP technique as a robust and rapid method to generate moderately saturated linkage maps, thereby allowing the molecular
analysis of traits, such as resistance to Fusarium wilt, that show oligogenic patterns of inheritance.
Received: 8 December 1997 / Accepted: 7 April 1998 相似文献
3.
Analysis of complex leaf and flower characters in Rhododendron using a molecular linkage map 总被引:10,自引:0,他引:10
F. Dunemann R. Kahnau I. Stange 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(6-7):1146-1155
A molecular linkage map of Rhododendron has been constructed by using a segregating population from an interspecific cross. Parent-specific maps based on 239 RAPD,
38 RFLP, and two microsatellite markers were aligned using markers heterozygous in both parents. The map of the male parent
‘Cunningham’s White’ comprised 182 DNA markers in 13 linkage groups corresponding to the basic chromosome number. In the female
parent ‘Rh 16’ 168 markers were located on 18 linkage groups. An assignment of putative homologous linkage groups was possible
for 11 groups of each parent. QTL analyses based on the non-parametric Kruskal-Wallis rank-sum test were performed for the
characters “leaf chlorosis” and “flower colour” scored as quantitative traits. For leaf chlorosis, two genomic regions bearing
QTLs with significant effects on the trait were identified on two linkage groups of the chlorosis-tolerant parent. RAPD marker
analysis of additional lime-stressed genotypes tested under altered environmental conditions verified the relationship between
marker allele frequencies and the expression of chlorosis. Highly significant QTL effects for flower colour were found on
two chromosomes indicating major genes located in these genome areas. The prospects for utilization of a linkage map in Rhododendron are discussed.
Received: 28 September 1998 / Accepted: 5 November 1998 相似文献
4.
A linkage map of the pea (Pisum sativum L.) genome containing cloned sequences of known function and expressed sequence tags (ESTs) 总被引:3,自引:0,他引:3
B. J. Gilpin J. A. McCallum T. J. Frew G. M. Timmerman-Vaughan 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(8):1289-1299
A linkage map of the pea (Pisum sativum L.) genome is presented which is based on F2 plants produced by crossing the marrowfat cultivar ‘Primo’ and the blue-pea breeding line ‘OSU442-15’. This linkage map consists
of 209 markers and covers 1330 cM (Kosambi units) and includes RFLP, RAPD and AFLP markers. By mapping a number of anchor
loci, the ‘Primo’בOSU442-15’ map has been related to other pea linkage maps. A feature of the map is the incorporation of
29 loci representing genes of known function, obtained from other laboratories. The map also contains RFLP loci detected using
sequence-characterized cDNA clones developed in our laboratory. The putative identities of 38 of these cDNA clones were assigned
by examining public-sequence databases for protein or nucleotide-sequence similarities. The conversion of sequence-characterized
pea cDNAs into PCR-amplifiable and polymorphic sequence-tagged sites (STSs) was investigated using 18 pairs of primers designed
for single-copy sequences. Eleven polymorphic STSs were developed.
Received: 18 June 1997 / Accepted: 11 August 1997 相似文献
5.
I. Eujayl M. Baum W. Powell W. Erskine E. Pehu 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(1-2):83-89
A genetic linkage map of Lens sp. was constructed with 177 markers (89 RAPD, 79 AFLP, six RFLP and three morphological markers) using 86 recombinant inbred
lines (F6:8) obtained from a partially interspecific cross. The map covered 1073 cM of the lentil genome with an average distance of
6.0 cM between adjacent markers. Previously mapped RFLP markers were used as anchor probes. The morphological markers, pod
indehiscence, seed-coat pattern and flower-color loci were mapped. Out of the total linked loci, 8.4% showed segregation distortion.
More than one-fourth of the distorted loci were clustered in one linkage group. AFLP markers showed more segregation distortion
than the RAPD markers. The AFLP and RAPD markers were intermingled and clustering of AFLPs was seldom observed. This is the
most extensive genetic linkage map of lentil to-date. The marker density of this map could be used for the identification
of markers linked to quantitative trait loci in this population.
Received: 6 November 1997 / Accepted: 10 February 1998 相似文献
6.
V. Katoch Susheel Sharma S. Pathania D. K. Banayal S. K. Sharma R. Rathour 《Molecular breeding : new strategies in plant improvement》2010,25(2):229-237
Powdery mildew caused by Erysiphe pisi D.C. is one of the most serious diseases that inflict heavy losses to pea crop world-wide. Identification of resistance sources
and their incorporation into susceptible cultivars remains the most effective method of controlling the disease. The present
study investigated the resistance phenotype, inheritance, and genomic location of gene(s) controlling resistance to powdery
mildew in pea genotype ‘JI2480’. The powdery mildew resistance in ‘JI2480’ appeared to be a spatial phenomenon showing expression
only in leaf tissues. By segregation analysis of an F2 progeny of cross ‘Lincoln/JI2480’, the leaf resistance of ‘JI2480’ was shown to be controlled by a single recessive gene,
presumed to be er2. Through linkage analysis of 111 resistant F2 progeny plants with simple sequence repeat (SSR) and random amplified polymorphic DNA (RAPD) markers adopted from the published
linkage maps, the er2 gene was localized on pea linkage group III (LGIII). The assignment of er2 to LGIII, a position different from that reported for er1, has resolved the long standing controversy in the literature regarding the existence and genomic location of er2 gene. A RAPD marker OPX-17_1400, exhibiting cis phase linkage (2.6 cM) to er2 was successfully converted to a sequence characterized amplified region (SCAR) marker, ScX17_1400. The SCAR marker ScX17_1400
will ensure speedy and precise introgression of er2 into susceptible cultivars by permitting selection of er2 heterozygotes amongst BC
n
F1s without progeny tests and resistance screening. 相似文献
7.
A genetic linkage map of tef [Eragrostis tef (Zucc.) Trotter] based on amplified fragment length polymorphism 总被引:2,自引:0,他引:2
G. Bai H. Tefera M. Ayele H. T. Nguyen 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(3-4):599-604
A genetic linkage map of tef was constructed with amplified fragment length polymorphism (AFLP) markers using F5 recombinant inbred lines (RILs) derived by single seed descent from the intraspecific cross of ’Kaye Murri’×’Fesho’. A total
of 192 EcoRI/MseI primer combinations were screened for parental polymorphism. Around three polymorphic fragments per primer combination were
detected, indicating a low polymorphism level in tef. Fifty primer combinations were selected to assay the mapping population,
and 226 loci segregated among 85 F5 RILs. Most AFLP loci behaved as dominant markers (presence or absence of a band), but about 15% of the loci were codominant.
Significant deviations from the expected Mendelian segregation ratio were observed for 26 loci. The genetic linkage map comprised
211 markers assembled into 25 linkage groups and covered 2,149 cM of genome. AFLP is an efficient marker system for mapping
plant species with low polymorphism such as tef. This is the first genetic linkage map constructed for tef. It will facilitate
the mapping of genes controlling agronomically important traits and cultivar improvement in tef.
Received: 27 April 1998 / Accepted: 4 January 1999 相似文献
8.
G. J. King F. H. Alston L. M. Brown E. Chevreau K. M. Evans F. Dunemann J. Janse F. Laurens J. R. Lynn C. Maliepaard A. G. Manganaris P. Roche H. Schmidt S. Tartarini J. Verhaegh R. Vrielink 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(5):699-708
Apple scab, caused by the fungus Venturia inaequalis (Cke.) Wint., is an important disease in commercial apple production. A mapping population of 155 individuals, derived from
a cross between the apple varieties ‘Prima’ (resistant)בFiesta’ (susceptible), was scored for response to the disease in
replicated field and glasshouse trials throughout Europe. Twenty data sets were selected and cluster analysis was used to
form a consensus score for the population fitting a 1 : 1 segregation ratio of resistance:susceptibility. The progeny were
scored with molecular markers. A detailed map covering 54 cM of the ‘Prima’ linkage group containing the Vf gene for scab resistance was constructed using 24 molecular markers linked to the resistance gene. One isoenzyme marker (Pgm-1), six RFLP markers and 17 RAPD markers formed a linkage group with the consensus measure of resistance to scab. Four marker
bridges were established with the corresponding ‘Fiesta’ linkage group with additional markers (one isozyme, one RFLP, three
RAPD and one AFLP). A low chi-square value indicated a good fit of the marker ordering, which was in close agreement with
previously reported linkage positions for some of the markers and Vf. Differences were observed in the ability of different scoring methods to resolve susceptible and resistant classes. The
results obtained for the consensus classification of resistance to scab for the population may suggest the presence of virulent
inocula at some sites, which could overcome the Vf gene for resistance. The consequences of relying on individual scoring occasions for studying Vf scab resistance are discussed in the context of linkage analysis, conventional breeding selection, and marker-assisted selection.
Received: 23 July 1997 / Accepted: 31 October 1997 相似文献
9.
Riaz S Krivanek AF Xu K Walker MA 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,113(7):1317-1329
A framework genetic map based on genomic DNA-derived SSR, EST-derived SSR, EST-STS and EST-RFLP markers was developed using 181 genotypes generated from D8909-15 (female) × F8909-17 (male), the ‘9621’ population. Both parents are half siblings with a common female parent, Vitis rupestris ‘A. de Serres’, and different male parents (forms of V. arizonica). A total of 542 markers were tested, and 237 of them were polymorphic for the female and male parents. The female map was developed with 159 mapped markers covering 865.0 cM with an average marker distance of 5.4 cM in 18 linkage groups. The male map was constructed with 158 mapped molecular markers covering 1055.0 cM with an average distance of 6.7 cM in 19 linkage groups. The consensus ‘9621’ map covered 1154.0 cM with 210 mapped molecular markers in 19 linkage groups, with average distance of 5.5 cM. Ninety-four of the 210 markers on the consensus map were new. The ‘Sex’ expression locus segregated as single major gene was mapped to linkage group 2 on the consensus and the male map. PdR1, a major gene for resistance to Pierce’s disease, caused by the bacterium Xylella fastidiosa, was mapped to the linkage group 14 between markers VMCNg3h8 and VVIN64, located 4.3 and 2.7 cM away from PdR1, respectively. Differences in segregation distortion of markers were also compared between parents, and three clusters of skewed markers were observed on linkage groups 6, 7 and 14. 相似文献
10.
V. Konduri I. D. Godwin C. J. Liu 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(6):866-871
A linkage map of Lablab purpureus consisting of 127 RFLP and 91 RAPD loci was constructed in an F2 population of 119 individuals. This population was derived from a cross between ’Rongai’ (an annual cultivar) and CPI 24973
(a perennial wild accession). The map comprises 17 linkage groups and covers 1610 centiMorgans (cM) with an average distance
of 7 cM between markers. Severe segregation distortions were observed, with the very extreme situation where no paternal type
was recovered from the mapping population. These results strongly suggest the presence of a gene conferring preferential transmission
from the maternal parent ’Rongai’. It was also clear that, while the majority of RAPD markers are valuable when used together
with RFLP or other stringent marker systems, they could be problematic when used solely in mapping exercises.
Received: 20 April 1999 / Accepted: 23 August 1999 相似文献
11.
J. B. Clarke D. J. Sargent R. I. Bošković A. Belaj K. R. Tobutt 《Tree Genetics & Genomes》2009,5(1):41-51
One hundred and sixty microsatellite (simple sequence repeat (SSR)) and six gene-specific markers revealing 174 loci were
scored in 94 seedlings from the inter-specific cross of Prunus avium ‘Napoleon’ × Prunus nipponica accession F1292. The co-segregation data from these markers were used to construct a linkage map for cherry which spanned
680 cM over eight linkage groups with an average marker spacing of 3.9 cM per marker and just six gaps longer than 15 cM.
Markers previously mapped in Prunus dulcis ‘Texas’ × Prunus persica ‘Earlygold’ allowed the cherry map to be anchored to the peach × almond map and showed the high level of synteny between
the species. Eighty-four loci segregated in P. avium ‘Napoleon’ versus 159 in P. nipponica. The segregations of 32 isoenzyme loci in a subset of 47 seedlings from the progeny were scored, using polyacrylamide gel
electrophoresis and/or isoelectric focusing separation followed by activity staining, and the co-segregation data were analysed
along with those for 39 isoenzymes reported previously and for the 174 sequence-tagged site loci plus an additional two SSR
loci. The second map incorporates 233 loci and spans 736 cM over eight linkage groups with an average marker spacing of 3.2 cM
per marker and just two gaps greater than 15 cM. The microsatellite map will provide a useful tool for cherry breeding and
marker-assisted selection and for synteny studies within Prunus; the gene-specific markers and isoenzymes will be useful for comparisons with maps of other rosaceous fruit crops.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
12.
Junke Zhang Ludger Hausmann Rudolf Eibach Leocir J. Welter Reinhard T?pfer Eva M. Zyprian 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,119(6):1039-1051
Grapevine rootstock cultivar ‘B?rner’ is a hybrid of Vitis riparia and Vitis cinerea Arnold that shows high resistance to phylloxera (Daktulosphaira vitifoliae Fitch). To localize the determinants of phylloxera root resistance, the susceptible grapevine V3125 (Vitis vinifera ‘Schiava grossa’ × ‘Riesling’) was crossed to ‘B?rner’. Genetic framework maps were built from the progeny. 235 microsatellite
markers were placed on the integrated parental map. They cover 1,155.98 cM on 19 linkage groups with an average marker distance
of 4.8 cM. Phylloxera resistance was scored by counting nodosities after inoculation of the root system. Progeny plants were
triplicated and experimentally infected in 2 years. A scan of the genetic maps indicated a quantitative trait locus on linkage
group 13. This region was targeted by six microsatellite-type markers newly developed from the V. vinifera model genome sequence. Two of these appear closely linked to the trait, and can be useful for marker-assisted breeding. 相似文献
13.
L. Gentzbittel E. Mestries S. Mouzeyar F. Mazeyrat S. Badaoui F. Vear D. Tourvieille de Labrouhe P. Nicolas 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(1-2):218-234
A map of the sunflower genome, based on expressed sequences and consisting of 273 loci, was constructed. The map incorporates
data from seven F2 populations, for a total of 1115 individuals. Two hundred and fourty five loci corresponding to 170 anonymous cDNA markers
and four loci for morphological markers were mapped. We also mapped 18 loci corresponding to previously described genes or
to sequences obtained through homology cloning. The unit maps vary from 774 cM to 1060 cM, with an average value of 14 major
linkage groups. The integrated map is arranged in 17 major linkage groups including 238 loci, plus four small segments with
2–5 marker loci; and covers 1573 cM with an overall average marker interval of 7 cM. Thirty five percent of the markers were
dominant in nature and 30% showed inter-linkage group duplication without any indication of homoeologous linkage groups. Evidence
is provided for the independence of two distinct fertility restoration genes, for the presence of two loosely linked branching
loci, and for marker tightly linked to the Rf1 restoration locus. This map provides an efficient tool in breeding applications such as disease-resistance mapping, QTL analyses
and marker-assisted selection.
Received: 27 August 1998 / Accepted: 28 December 1998 相似文献
14.
Genetics of resistance to ascochyta blight (Ascochyta lentis) of lentil and the identification of closely linked RAPD markers 总被引:1,自引:0,他引:1
R. Ford E. C. K. Pang P. W. J. Taylor 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(1):93-98
Foliar resistance to Ascochyta lentis is controlled at a single major locus by a dominant gene (AbR
1
) in the lentil accession ILL5588 (cv ‘Northfield’). Flanking RAPD markers that are closely linked to the resistance locus
in coupling phase were identified by bulked segregant analysis. Out of 261 decanucleotide primers screened 7 produced a polymorphic
marker that segregated with the resistance locus, and all markers were found to exist within a single linkage group. Five
of the seven RAPD markers were within 30 cM of the resistance locus. Log likelihood analysis for detecting QTL associated
with the foliar resistance revealed that a single narrow peak accounted for almost 90% of the variance of resistance between
the bulks. Preliminary mapping in an F3 population revealed that the closest flanking markers were approximately 6 and 14 centiMorgans (cM) away from the resistance
locus. These markers should be useful for the discrimination of resistant germplasm through marker-assisted selection in future
breeding programmes and represent the first essential step towards the map-based cloning of this resistance gene.
Received: 18 December 1997 / Accepted: 9 June 1998 相似文献
15.
V. Laporte D. Merdinoglu P. Saumitou-Laprade G. Butterlin P. Vernet J. Cuguen 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(6-7):989-996
The present study shows that the recently described mitochondrial H haplotype is associated with cytoplasmic male-sterility
(CMS). This new source of CMS appears to be different from the mitotype E-associated CMS most frequently found in natural
populations. A mitotype H progeny with a sexual phenotype segregation was used to identify a gene restoring male fertility
(R1H ). Using bulk segregant analysis (BSA), nine RAPD markers linked to this restorer locus were detected and mapped. The comparison
with other Beta genetic maps shows that the closest RAPD marker, distant from R1H by 5.2 cM, belongs to the same linkage group as the monogermy locus. In order to determine the position of R1H more precisely, four RFLP loci within this linkage group were mapped in the segregating progeny. It thus became possible
to construct a linkage map of the region containing the RFLP, RAPD and R1H loci. The closest RFLP marker was located 1.7 cM away from R1H. However, a nuclear gene restoring the ‘Owen’ CMS which is currently used in sugar beet breeding is reportedly linked to
the monogermy locus, raising the question of a possible identity between the new CMS system and the ‘Owen’ CMS.
Received: 15 September 1997 / Accepted: 1 December 1997 相似文献
16.
Development of a genetic linkage map and identification of homologous linkage groups in sweetpotato using multiple-dose AFLP markers 总被引:1,自引:0,他引:1
Jim C. Cervantes-Flores G. Craig Yencho Albert Kriegner Kenneth V. Pecota Maria A. Faulk Robert O. M. Mwanga Bryon R. Sosinski 《Molecular breeding : new strategies in plant improvement》2008,21(4):511-532
Sweetpotato genomic research is minimal compared to most other major crops despite its worldwide importance as a food crop.
The development of a genetic linkage map in sweetpotato will provide valuable information about the genomic organization of
this important species that can be used by breeders to accelerate the introgression of desired traits into breeding lines.
We developed a mapping population consisting of 240 individuals of a cross between ‘Tanzania’, a cream-fleshed African landrace,
and ‘Beauregard’, an orange-fleshed US sweetpotato cultivar. The genetic linkage map of this population was constructed using
Amplified Fragment Length Polymorphism (AFLP) markers. A total of 1944 (‘Tanzania’) and 1751 (‘Beauregard’) AFLP markers,
of which 1511 and 1303 were single-dose markers respectively, were scored. Framework maps consisting of 86 and 90 linkage
groups for ‘Tanzania’ and ‘Beauregard’ respectively, were developed using a combination of JoinMap 3.0 and MAPMAKER/EXP 3.0.
A total of 947 single-dose markers were placed in the final framework linkage map for ‘Tanzania’. The linkage map size was
estimated as 5792 cM, with an average distance between markers of 4.5 cM. A total of 726 single-dose markers were placed in
the final framework map for ‘Beauregard’. The linkage map length was estimated as 5276 cM, with an average distance between
markers of 4.8 cM. Duplex and triple-dose markers were used to identify the corresponding homologous groups in the maps. Our
research supports the hypothesis that sweetpotato is an autopolyploid. Distorted segregation in some markers of different
dosages in this study suggests that some preferential pairing occurs in sweetpotato. However, strict allopolyploid inheritance
in sweetpotato can be ruled out due to the observed segregation ratios of the markers, and the proportion of simplex to multiple-dose
markers.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
This paper is a portion of a dissertation submitted by Jim C. Cervantes-Flores. 相似文献
17.
A genetic linkage map of durum wheat 总被引:20,自引:6,他引:14
A. Blanco M. P. Bellomo A. Cenci C. De Giovanni R. D’Ovidio E. Iacono B. Laddomada M. A. Pagnotta E. Porceddu A. Sciancalepore R. Simeone O. A. Tanzarella 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(5-6):721-728
A genetic linkage map of tetraploid wheat [Triticum turgidum (L.) Thell.] was constructed using segregation data from a population of 65 recombinant inbred lines (RILs) derived from
a cross between the durum wheat cultivar Messapia and accession MG4343 of T. turgidum (L.) Thell. ssp dicoccoides (Korn.) Thell. A total of 259 loci were analysed, including 244 restriction fragment length polymorphisms (RFLPs), one PCR
(polymerase chain reaction) marker (a sequence coding for a LMW (low-molecular-weight) glutenin subunit gene located at the
Glu-B3 locus), seven biochemical (six seed-storage protein loci and one isozyme locus) and seven morphological markers. A total
of 213 loci were mapped at a LOD≥3 on all 14 chromosomes of the A and B genomes. The total length of the map is 1352 cM and
the average distance between adjacent markers is 6.3 cM. Forty six loci could not be mapped at a LOD≥3. A fraction (18.6%)
of the markers deviated significantly from the expected Mendelian ratios; clusters of loci showing distorted segregation were
found on chromosomes 1B, 3AL, 4AL, 6AL and 7AL. The durum wheat map was compared with the published maps of bread wheat using
several common RFLP markers and general features are discussed. The markers detected the known structural rearrangements involving
chromosomes 4A, 5A and 7B as well as the translocation between 2B-6B, but not the deletion on 2BS. This map provides a useful
tool for analysing and breeding economically important quantitative traits and for marker-assisted selection, as well as for
studies of genome organisation in small grain cereal species.
Received: 5 January 1998 / Accepted: 31 March 1998 相似文献
18.
A genetic map of Asparagus officinalis based on integrated RFLP, RAPD and AFLP molecular markers 总被引:6,自引:0,他引:6
A. Spada E. Caporali G. Marziani P. Portaluppi F. M. Restivo F. Tassi A. Falavigna 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(7):1083-1089
An integrated genetic map of the dioecious species Asparagus officinalis L. has been constructed on the basis of RFLP, RAPD, AFLP and isoenzyme markers. The segregation analysis of the polymorphic
markers was carried out on the progeny of five different crosses between male and female doubled-haploid clones generated
by anther culture. A total of 274 markers have been organized to ten linkage groups spanning 721.4 cM. Since the haploid chromosome
number of asparagus is ten, the established linkage groups probably represent the different chromosomes; however, the only
group associated with a specific chromosome is the one which includes sex, whose determinant genes have been located on chromosome
5. A total of 33 molecular markers (13 RFLPs, 18 AFLPs, 2 RAPDs and 1 isoenzyme) have been located on this chromosome. The
closest marker to the sex determinant is the AFLP SV marker at 3.2 cM.
Received: 26 March 1998 / Accepted: 30 April 1998 相似文献
19.
Fernando J. Yuste-Lisbona Carmen Capel Emilio Sarria Rocío Torreblanca María L. Gómez-Guillamón Juan Capel Rafael Lozano Ana I. López-Sesé 《Molecular breeding : new strategies in plant improvement》2011,27(2):181-192
Powdery mildew caused by Podosphaera xanthii has become a major problem in melon since it occurs all year round irrespective of the growing system. The TGR-1551 melon
genotype was found to be resistant to several melon diseases, among them powdery mildew. However, the corresponding resistance
genes have been never mapped. We constructed an integrated genetic linkage map using an F2 population derived from a cross
between the multi-resistant genotype TGR-1551 and the susceptible Spanish cultivar ‘Bola de Oro’. The map spans 1,284.9 cM,
with an average distance of 3.6 cM among markers, and consists of 354 loci (188 AFLP, 39 RAPD, 111 SSR, 14 SCAR/CAPS/dCAPS,
and two phenotypic traits) distributed in 14 linkage groups. QTL analysis identified one major QTL (Pm-R) on LG V for resistance to races 1, 2, and 5 of powdery mildew. The PM4-CAPS marker is closely linked to the Pm-R QTL at a genetic distance of 1.9 cM, and the PM3-CAPS marker is located within the support interval of this QTL. These codominant
markers, together with the map information reported here, could be used for melon breeding, and particularly for genotyping
selection of resistance to powdery mildew in this vegetable crop species. 相似文献
20.
K. D. Jermstad D. L. Bassoni N. C. Wheeler D. B. Neale 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(5-6):762-770
We have constructed a sex-averaged genetic linkage map in coastal Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco var ‘menziesii’) using a three-generation outcrossed pedigree and molecular markers. Our research objectives are to learn about genome organization
and to identify markers associated with adaptive traits. The map reported here is comprised of 141 markers organized into
17 linkage groups and covers 1,062 centiMorgans (cM). Of the markers positioned on the map, 94 were derived from a Douglas-fir
complimentary-DNA (cDNA) library that was constructed from new-growth needle tissue. Other markers include 11 Douglas-fir
genomic-DNAs, 20 loblolly pine (Pinus taeda L.) cDNAs, 15 random amplified polymorphic DNAs (RAPDs) and a PCR-amplified phytochrome probe. A high degree of variation
was detected in each of the two parents of our mapping population, and many of the restriction fragment length polymorphism
(RFLP) and RAPD phenotypes were complex. Marker data were analyzed for linkage using mapping software JOINMAP version 2.0.
Received: 16 March 1998 / Accepted: 22 April 1998 相似文献