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1.
前体饲喂、诱导子和光照联合使用对葡萄细胞培养合成花青素的影响 总被引:4,自引:0,他引:4
苯丙氨酸前体饲喂分别和环糊精、葡聚糖、茉莉酸甲酯、黑曲霉和直喙镰孢菌提取液五种诱导子联合作用,其中以与茉莉酸甲酯的联合作用对葡萄细胞培养生产花青素的影响最大,可使单位鲜细胞花青素含量提高2.7倍,花青素产量提高3.4倍,实验证明两者在培养后第4天加入效果最好。在30μmol/L苯丙氨酸、218μmol/L茉莉酸甲酯和3000~4000lx光照条件下,不同花青素产量的细胞株都能显著提高花青素产量,但低产株VV06比高产株VV05具有更大的产率提高潜力。该条件下VV05和VV06花青素产量分别达到2975和4090CV/L,是对照组的2.5倍和5.2倍。 相似文献
2.
Junge Qu Wei Zhang Xingju Yu Meifang Jin 《Biotechnology and Bioprocess Engineering》2005,10(2):155-161
The inherent instability of metabolite production in plant cell culture-based bioprocessing is a major problem hindering its
commercialization. To understand the extent and causes of this instability, this study was aimed at understanding the variability
of anthocyanin accumulation during long-term subcultures, as well as within subculture batches, inVitis vinifera cell cultures. Therefore, four cell line suspensions ofVitis vinifera L. var. Gamay Fréaux, A, B, C and D, originated from the same callus by cell-aggregate cloning, were established with starting
anthocyanin contents of 2.73±0.15, 1.45±0.04, 0.77±0.024 and 0.27±0.04 CV (Color Value)/g-FCW (fresh cell weight), respectively.
During weekly subculturing of 33 batches over 8 months, the anthocyanin biosynthetic capacity was gradually lost at various
rates, for all four cell lines, regardless of the significant difference in the starting anthocyanin content. Contrary to
this general trend, a significant fluctuation in the anthocyanin content was observed, but with an irregular cyclic pattern.
The variabilities in the anthocyanin content between the subcultures for the 33 batches, as represented by the variation coefficient
(VC), were 58, 57, 54, and 84% forV. vinifera cell lines A, B, C and D, respectively. Within one subculture, the VCs from 12 replicate flasks for each of 12 independent
subcultures were averaged, and found to be 9.7%, ranging from 4 to 17%. High- and low-producing cell lines, VV05 and VV06,
with 1.8-fold differences in their basal anthocyanin contents, exhibited different inducibilities tol-phenylalanine feeding, methyl jasmonate and light irradiation. The low-producing cell line showed greater potential in enhanced
the anthocyanin production. 相似文献
3.
Milen I. Georgiev Sonya L. Kuzeva Atanas I. Pavlov Elena G. Kovacheva Mladenka P. Ilieva 《World journal of microbiology & biotechnology》2007,23(2):301-304
The growth of Lavandula vera MM plant cell suspension culture and rosmarinic acid biosynthesis under elicitation with benzothiadiazole and methyl jasmonate
were investigated. Upon elicitation with 50 μM methyl jasmonate, the production of rosmarinic acid was enhanced 2.4-fold (3348 mg/l)
compared to the non-elicited cells. The influence of benzothiadiazole on rosmarinic acid biosynthesis was weaker and 12 h
after its addition the achieved yields were 20–30% higher compared to the control variant at this time. The influence of both
elicitors on rosmarinic acid secretion into the culture medium was also discussed. 相似文献
4.
Latiporn Udomsuk Kanokwan Jarukamjorn Hiroyuki Tanaka Waraporn Putalun 《Biotechnology letters》2011,33(2):369-374
The effect of abiotic and biotic elicitors (methyl jasmonate, chitosan, salicylic acid, Agrobacterium, and yeast extract) at various concentrations on total isoflavonoid accumulation was studied in the hairy root cultures of
Pueraria candollei. All elicitors stimulated isoflavonoid production. Yeast extract (0.5 mg/ml) was the most efficient giving total isoflavonoids
at 60 ± 1 mg/g dry wt, which was 4.5-fold higher than control hairy roots on day 3 of elicitation. 相似文献
5.
Zhenzhen Cai Anja Kastell Inga Mewis Dietrich Knorr Iryna Smetanska 《Plant Cell, Tissue and Organ Culture》2012,108(3):401-409
The effects of yeast extract and selected polysaccharide elicitors on secondary metabolite production, particularly of anthocyanin
and phenolic acid, in cell suspension cultures of Vitis vinifera were investigated. All elicitors either maintained or promoted cell growth in culture. Overall, secondary metabolite production
in V. vinifera cell suspension cultures responded differently to different elicitors. Chitosan, pectin, and alginate enhanced production
of anthocyanin within 13 days of culture with levels of 2.5-, 2.5-, and 2.6-fold increase, respectively, over that of control.
Chitosan, alginate, and gum arabic significantly promoted accumulation of phenolic acids, particularly 3-O-glucosyl-resveratrol, in V. vinifera cultures, as well as in the culture medium. Intracellular phenolic acid production was significantly enhanced by alginate
and chitosan, with 1.7- and 1.5-fold levels, respectively, of that of control. Extracellular phenolic acid production was
also significantly increased in the presence of chitosan and gum arabic, with levels of 3.3- and 1.7-fold higher, respectively,
than those of control. In addition, DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity was enhanced in the presence
of elicitors, and this was positively correlated with increased accumulation of anthocyanin in V. vinifera cell suspension cultures. 相似文献
6.
Cell suspension cultures of Gymnema sylvestre treated with four different elicitors, methyl jasmonate (MJ), yeast extract, chitin and pectin were studied for the production
of gymnemic acid as gymnemagenin equivalent, that was analyzed by high performance liquid chromatography (HPLC). All the four
tested elicitors induced gymnemic acid production in cell suspension cultures. Highest gymnemic acid content was achieved
following treatment with yeast extract (100.47 ± 0.28 mg/l), this was followed by MJ (70.43 ± 0.26 mg/l), pectin (64.19 ± 0.23 mg/l)
and chitin (62.72 ± 0.13 mg/l). The addition of elicitors has shown a significant influence on cell growth that affected cell
growth compared to respective controls. The highest gymnemic acid production was obtained after 20 days of elicitation in
cultures treated with 0.5 g l−l yeast extract, it was 5.25-folds greater than in control. These results suggest that the addition of an elicitor to Gymnema sylvestre cell suspension cultures could stimulate and enhance gymnemic acid production. In our present study we could able to overproduce
gymnemic acid up to 51.97 ± 0.26 mg l−l (dry weight basis) in yeast extract treated cell suspension cultures. 相似文献
7.
Effects of exogenous methyl jasmonate in elicited anthocyanin-producing cell cultures of ohelo (Vaccinium phalae) 总被引:1,自引:0,他引:1
Yimin Fang M. A. L. Smith M. -F. Pépin 《In vitro cellular & developmental biology. Plant》1999,35(1):106-113
Summary Elicitation of anthocyanin-producing cells of ohelo (Vaccinium pahalae) by both biotic (purified β-glucan and chitosan) and abiotic [sodium ferric ethylenediamine di-(o-hydroxyphenylacetate) FeEDDHA, and CuSO4] elicitors resulted in significant enhancement of anthocyanin accumulation. Anthocyanin production increased up to 1.8 and
1.5-fold over the control in the presence of abiotic elicitors (90 μM FeEDDHA and 20 μM CuSO4, respectively), and increased 1.9 and 1.6-fold in the presence of biotic elicitors (10 mg L−1 β-glucan and 100 mg L−1 chitosan). Maximum anthocyanin production with the two most effective elicitors was achieved when cultures were treated on
Day 3 (β-glucan) or Day 0 (FeEDDHA) after the initiation of fresh cell cultures. A concentration-dependent response was exhibited
by cultures treated with exogenous methyl jasmonate (MJ). The addition of 0.5 μM MJ alone provoked a 2–3-fold increase in anthocyanin production over that of the control; however, no additive effect on
anthocyanin production was observed in any treatments which combined MJ and β-glucan or FeEDDHA. Conditioning of the cells
with a preculture in either MJ, β-glucan, or FeEDDHA similarly did not enhance anthocyanin production. Inoculation of cultures
elicited by MJ or β-glucan with ibuprofen, a reported inhibitor of jasmonate biosynthesis, dramatically stimulated, rather
than inhibited, anthocyanin production, resulting in levels of accumulation beyond any of the tested elicitor combinations.
Hypotheses for the observed influence of ibuprofen in this system are discussed. 相似文献
8.
Ming-Bo Gao Wei Zhang Cheng-jiang Ruan 《World journal of microbiology & biotechnology》2011,27(10):2271-2279
A highly efficient process intensification strategy was developed to enhance the taxuyunnanine C (Tc) production in Taxus chinensis (T. chinensis) by combining repeated elicitation with a synthesized jasmonate analog, 2,3-dihydroxypropyl jasmonate (DHPJA), sucrose feeding
and in situ adsorption. Suspension cultures of a high Tc-producing cell line of T. chinensis were supplemented twice, on day 7 and day 12, with 100 μM DHPJA. The cultures were also supplemented by feeding 20 g sucrose/l
and 100 g Ambelite XAD-7/l on day 7. This strategy had a synergistic effect on Tc synthesis, causing a significant increase
in the Tc yield to 1,715.13 ± 124.12 mg/l on day 21. The yield was 3.28-fold above that produced by repeated DHPJA elicitation
combined with sucrose feeding. When XAD-7 was replaced by Amberlite XAD-7 HP, the result was slightly different. The highest
Tc yield of 1,728.84 ± 50.37 mg/l was achieved on day 27. The Tc yield achieved here is greater than twice the highest yield
reported previously, the sole reported taxoid production yield above the gram per liter level. Given the pharmaceutical importance
of taxoids, the results of this study will greatly contribute to the potential industrial production of Tc and other taxoids
by plant cell cultures. 相似文献
9.
The effects of methyl jasmonate and jasmonic acid on galanthamine production, phenolic acid content and growth of Leucojum aestivum L. shoot culture, cultivated in submerged conditions were investigated. The best time-point for addition of elicitors was during the exponential phase of the culture growth. The maximal contents of galanthamine and lycorine (226.9 μg/flask and 491.4 μg/flask, 1.36 and 1.67-fold higher compared to the control, respectively) were achieved after elicitation with jasmonic acid, whereas the elicitation with methyl jasmonte resulted in maximal accumulation of phenolic acids. It was demonstrated that the boosting effect of jasmonic acid on Amaryllidacea alkaloid biosynthesis was due to induction of the activity of tyrosine decarboxylase, whereas methyl jasmonate stimulates the biosynthesis of phenolic acids by inducing mainly the activity of phenylalanine ammonia-lyase. 相似文献
10.
The Root cultures of Cayratia trifolia (Vitaceae) a tropical lianas, were maintained in liquid Murashige and Skoog’s medium containing 0.5 mg l−1 NAA, 0.1 mg l−1 kinetin with 3% sucrose. These root cultures when grown with 6% sucrose accumulated stilbenes (piceid, resveratrol, viniferin,
ampelopsin) in high amounts, which on elicitation by 500 mg l−1 yeast extract, 50 μM salicylic acid (SA), 50 μM methyl jasmonate (MeJa), 500 μM ethrel added at 25th day, increased up to
ninefolds (7.1 mg l−1). Addition of alar or phenylalanine along with the elicitors further enhanced the stilbenes content. In the present study,
stilbenes accumulation up to 12 folds (9.2 mg l−1) was obtained with SA and alar. The SA was the most effective in increasing the stilbenes contents while less than control
values were recorded in the cells treated with MeJa. The roots could be grown up to 2 l flasks. The present work demonstrates
that presence of precursor and sucrose during elicitation at an appropriate time combined with growth retardation significantly
increased the production of stilbenes in C. trifolia cell cultures. 相似文献
11.
细胞均一性对葡萄细胞生长和花青素合成的影响 总被引:1,自引:1,他引:0
通过色差筛选法建立了一个相对均一的葡萄细胞悬浮系E,其细胞团较小,在长期继代培养过程中花青素合成能力的变异系数为8.7%,重复摇瓶实验的变异系数为5%。以E为实验材料进行的各组前体饲喂、诱导子添加、光照等联合作用实验,其生物量和花青素合成的变异系数均可控制在12%以内,充分说明了培养体系的均一性对维持稳定生产的重要性;黑暗条件下添加30μmol/L苯丙氨酸(Phe)和218μmol/L茉莉酸甲酯(MeJA)可使单位细胞花青素含量达到对照组的5.89倍,花青素产量为对照组的4.30倍,且连续5次继代培养过程中生物量和花青素合成的变异系数均比对照组降低。 相似文献
12.
Amit N. Shinde Nutan Malpathak Devanand P. Fulzele 《Biotechnology and Bioprocess Engineering》2009,14(3):288-294
The effect of biotic elicitors (yeast extract, chitosan), signaling molecule (salicylic acid), and polyamines (putrescine
and spermidine) was studied with respect to isoflavones accumulation in hairy root cultures of Psoralea corylifolia L. Untreated hairy roots (control) accumulated 1.55% dry wt of daidzein and 0.19% dry wt of genistein. In precursor feeding
experiment, phenylalanine at 2 mM concentration led to 1.3 fold higher production of daidzein (1.91% dry wt) and genistein
(0.27% dry wt). In biotic elicitors, chitosan (2 mg/L) was found to be the most efficient elicitor to induce daidzein (2.78%
dry wt) and genistein (0.279% dry wt) levels in hairy roots. Salicylic acid at 1 mM concentration stimulated the maximum accumulation
of daidzein (2.2% dry wt) and genistein (0.228% dry wt) 2 days after elicitation. In case of polyamines, putrescine (50 mM)
resulted in highest accumulation of daidzein (3.01% dry wt) and genistein (0.227% dry wt) after 5 days of addition. Present
results indicated the effectiveness of elicitation and precursor feeding on isoflavones accumulation in hairy roots of P. corylifolia. This is the first report of elicitation on isoflavones production by hairy roots of P. corylifolia. 相似文献
13.
Cheryl R. Frankfater Michael K. Dowd Barbara A. Triplett 《Plant Cell, Tissue and Organ Culture》2009,98(3):341-349
The effect of two chemical elicitors, salicylic acid and methyl jasmonate, on the production of gossypol, 6-methoxygossypol,
and 6,6′-dimethoxygossypol in Gossypium barbadense hairy roots was examined. Methyl jasmonate, but not salicylic acid, was found to increase the production of gossypol and
its methylated forms, but with a concomitant reduction in culture growth. The optimal methyl jasmonate dose was between 100
and 300 μM for hairy roots harvested 7 days after elicitation. After 20 d of induction with 100 μM methyl jasmonate, an eightfold
increase in the level of gossypol was observed in elicited cultures compared with control cultures, double the highest gossypol
levels previously reported for any cotton tissue. A two to threefold increase in the level of 6-methoxygossypol and a slight
increase in the levels of 6,6′-dimethoxygossypol were also observed. Although methyl jasmonate stimulated the production of
both optical forms of gossypol, the distribution of the enantiomers was different between elicited and control cultures. 相似文献
14.
Justyna Krzyzanowska Anna Czubacka Lukasz Pecio Marcin Przybys Teresa Doroszewska Anna Stochmal Wieslaw Oleszek 《Plant Cell, Tissue and Organ Culture》2012,108(1):73-81
The effects of two elicitors: jasmonic acid and methyl jasmonate on cell growth as well as on rosmarinic acid accumulation
in cell suspension cultures of Mentha × piperita were investigated. The highest rosmarinic acid accumulation 117.95 mg g−1 DW (12% DW) was measured 24 h after addition of 100 μM methyl jasmonate. A similar concentration 110.12 mg g−1 DW was detected 48 h after application of 200 μM jasmonic acid. Those values were nearly 1.5 times higher compared to the
control sample, without elicitation. There was no substantial influence of elicitors on rosmarinic acid secretion into the
culture media. Extracellular concentrations of rosmarinic acid were similar to the values from the control variants. It was
documented that suspension cultures of M. piperita treated with elicitors showed a decrease in biomass accumulation when compared to the control. 相似文献
15.
Amit N. Shinde Nutan Malpathak Devanand P. Fulzele 《Biotechnology and Bioprocess Engineering》2009,14(5):612-618
The effect of biotic elicitors (yeast extract, chitosan), signaling molecule (salicylic acid), and polyamines (putrescine
and spermidine) was studied with respect to isoflavones accumulation in cell suspension cultures of corylifolia L. Untreated cell suspension (control) accumulated 1.66% dry wt of daidzein and 0.165% dry wt of genistein. In precursor
feeding experiment, phenylalanine at 0.5 mM concentration led to 1.3 fold higher production of daidzein (1.99% dry wt) and
genistein (0.22% dry wt). In biotic elicitors, yeast extract (100 mg/L) was found to be the most efficient elicitor to induce
higher production levels of daidzein (2.21% dry wt) and genistein (0.293% dry wt) in suspension cultures. Salicylic acid (signaling
molecule) at 1 mM concentration stimulated the maximum accumulation of daidzein (3.4% dry wt) and genistein (0.41% dry wt)
2 days after elicitation. In case of polyamines, spermidine (100 mM) resulted in highest accumulation of daidzein (3.2% dry
wt) and genistein (0.475% dry wt) after 7 days of addition, which was 2.4 fold of that in control. This is the first report
on kinetics of isoflavone production in response to elicitation in cell suspension of P. corylifolia. 相似文献
16.
Hong Soon Rhee Hwa-Young Cho Seok Young Son Sung-Yong H. Yoon Jong Moon Park 《Plant Cell, Tissue and Organ Culture》2010,101(3):295-302
Angelica gigas root cultures were elicited with various elicitors, including yeast extract, chitin, methyl jasmonate, salicylic acid, and
copper, with the aim of increasing the production of decursin and decursinol angelate. The treatment of A. gigas root cultures with a combination of yeast extract (2 g l−1) and copper ion (0.5 mM) at the late exponential growth phase increased decursinol angelate accumulation up to 6.86 mg l−1. The best elicitor preparation selected through in vitro experiments was also applied to roots of A. gigas whole plants grown in the field in order to investigate the potential of elicitation as a novel cultivation practice for
producing medicinal herbs of improved quality. Biweekly treatments with the elicitor at 70 mg g l−1 FW roots for 10 weeks before the annual harvest resulted in an increment in both plant yields and specific productivity of
decursins by 1.5- and 1.7-fold, respectively. This result implies that in vitro screening of elicitors with the ultimate aim
of in planta elicitation of whole plants could be effective in terms of time and expense. The elicitation technique reported
here demonstrates it potential as a strategy for improving growth and active compounds productivity of medicinal plants through
short-term and pre-harvest treatment of the elicitor preparation. 相似文献
17.
We have studied the effects of precursor amino acids (phenylalanine and cystein), elicitors (salicylic and acetylsalicylic acids, methyl jasmonate, β-aminobutyric acid, yeast extract), PAL-inhibitor (1-amino-2-phenylethylphosphonic acid) applied alone or in combination on glucotropaeolin production and myrosinase activity in hairy root cultures of Tropaeolum majus. Short, 24-h treatment, and subsequent transfer of hairy roots to the fresh medium enabled avoiding detrimental effect of studied stimulators on biomass growth. In control cultures the highest glucotropaeolin content, 58.1 ± 6.7 mg g−1 DW, was detected on the 3rd day after transfer of the roots to the fresh medium but glucotropaeolin yield (mg 100 ml–1 culture volume) had been increasing until the 9th day after transfer as a result of continuous biomass growth. Glucotropaeolin content and yield were 2-fold enhanced after treatment with precursor amino acids or PAL-inhibitor alone, but their combination additively led to 4-fold increase in glucotropaeolin production. Among the studied elicitors acetylsalicylic acid induced the highest, 3-fold increase in glucotropaeolin production, it also enhanced myrosinase activity, but to a smaller extend (by about 50%). Acetylsalicylic acid also potentiated induced by precursors, PAL-inhibitor, methyl jasmonate and yeast extract production of glucotropaeolin. The highest, 4.8-fold increase in glucotropaeolin production was found after combined acetylsalicylic acid and precursors treatment. Additive effect of acetylsalicylic acid-combined treatment on myrosinase activity was not detected. The obtained results indicate that amino acid precursors, phenylalanine and cystein, availability may be a limiting factor in the process of stimulation of glucotropaeolin production in T. majus hairy root cultures. 相似文献
18.
G. Suvarnalatha L. Rajendran G. A. Ravishankar L. V. Venkataraman 《Biotechnology letters》1994,16(12):1275-1280
Summary A cell line of carrot (Daucus carota L) which produces anthocyanin was subjected to various elicitors and abiotic stresses: The elicitors tested were culture filtrates (CF) and cell extracts (CE) of certain bacteria and yeasts. The abiotic stresses were salts of certain metal ions. The production increase obtained with cell extracts of Bacillus cereus. Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus were 49, 72, 45 and 41% respectively over the control. Maximum elicitation was obtained with elicitor derived from cell extract of the yeast Rhodotorula rubra where it enhanced anthocyanin production by two fold. The abiotic stress agents Ca, Mn, Zn, Co, Fe & V enhanced anthocyanin production. Of all the metal ions tested Ca was the most effective. The elicitation process was governed by the type and level of elicitor. 相似文献
19.
A new 10-hydroxycamptothecin (HCPT)-producing fungus was isolated from Camptotheca
acuminata. The strain was classified as a Xylaria sp. based on the internal transcribed spacer and 18S rDNA gene analysis. All elicitors tested, except methyl jasmonate, increased
HCPT production in submerged culture. The maximum yield was 5.4 mg HCPT/l−1, when salicylic acid was added at 0.1 mM to the culture medium 相似文献