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1.
Nematospiroides dubius: passive transfer of protective immunity to mice with monoclonal antibodies 总被引:1,自引:0,他引:1
I J East E A Washington P J Brindley G F Monroy N Scott-Young 《Experimental parasitology》1988,66(1):7-12
Nine hybridoma cell lines secreting monoclonal antibodies specific for Nematospiroides dubius were produced by fusion of the mouse myeloma cell line NS-1 to either spleen cells or mesenteric lymph node cells from mice repeatedly infected with N. dubius. Seven of the antibodies were identified as IgM and two as IgG1. Each monoclonal antibody bound to polypeptide epitopes on both infective larvae (L3) and adult worms. However, five antibodies bound preferentially to L3 and three to adult worms. All nine antibodies reacted with high molecular weight protein antigens. Passive protective immunity in Balb/c mice was demonstrated with monoclonal antibodies Nd2 and Nd3 in ascites fluid which stunted both male and female worms and reduced parasite fecundity. 相似文献
2.
Allogeneic mice were reared over 5 generations in a 2-way selection for high (H) and low (L) immune responsiveness to the intestinal trichostrongylid nematode Nematospiroides dubius. After 5 generations of selective breeding, the H mice passed fewer N. dubius eggs and harbored fewer, smaller and less fecund worms than did the L mice. These trends were observed after each of 4 consecutive infections, which were all treated with levamisole before reinfection. Further, more H than L mice were worm-free, and H mice were sensitized to sterile immunity by fewer infections than were needed by the L mice. These results encourage the view that the selective breeding of host species of agricultural importance is a valuable means of biologic control of nematodiasis. 相似文献
3.
Test mice have been selectively reared for high (H) or low (L) immune responses to Nematospiroides dubius. After secondary infection with N. dubius, the L mice voided ten times as many eggs in their faeces as the H mice, and at necropsy, 71% versus 20% of the inoculum of N. dubius were recovered as adult worms from the L and H mice respectively. Furthermore, N. dubius were more fecund in the L than in H mice. High or low immune responsiveness was not restricted to N. dubius infection in these mice but was also observed during Toxocara canis infection. The migration of T. canis larvae from gut via the liver to skeletal muscle and CNS was inhibited in H versus L mice. Many more larvae were recovered from the livers of H compared with L mice which was indicative of greater immunity in the H mice. The protective immune response in H compared with L mice to both N. dubius and T. canis included pronounced eosinophilia and elevated antiparasite antibody titres. 相似文献
4.
D C Jenkins 《Parasitology》1975,71(2):349-355
The fecundity and longevity of Nippostrongylus brasiliensis was prolonged in mice previously infected with Nematospiroides dubius only when the former developed from the larval stage in those mice. Such worms appeared to be less immunogenic than worms which developed in mice never exposed to N. dubius. It is proposed that prolonged fecundity and longevity resulted from an adaptation undertaken by the worms in the face of host antibodies which had been developed against the pre-existing N. dubius infection. 相似文献
5.
The selection of mice for innate immunity to Nematospiroides dubius was not specific. Mice bred as refractory (R) to N. dubius infection were more refractory to primary infections with Nippostrongylus brasiliensis than other mice bred as liable (L) to infection with N. dubius. R and L, as well as randomly-bred (Rd) and inbred C3H mice were all immune to challenge infection with N. brasiliensis. Previous infections with N. brasiliensis failed to influence the course of N. dubius infections or the status of the selected mice as R, Rd and L to infection with N. dubius. R and L mice were equally susceptible to Mesocestoides corti infection but more resistant than Rd mice. R and L mice died sooner after infection with Salmonella typhimurium than Rd, although R survived longer than L mice. 相似文献
6.
High and low immune responder lines of mice were bred selectively from an allogeneic stock over 10 generations, based on their fecal parasite egg count assayed 3 weeks after reinfection with 100 Nematospiroides dubius larvae. By generation 10, (F10), the low immune response mice voided about 10 times as many fecal N. dubius eggs as the high immune response mice. Realized heritability for the selected trait, fecal egg count after secondary infection (= protective immunity), was 0.35 at F7. F7 was considered the selection limit. Selection for change in fecal egg count did not significantly influence the conformational nor reproductive characteristics of these mice. Significant phenotypic and genetic correlations were evident between the selected character and innate immunity to N. dubius, humoral antibody response to N. dubius infection, and establishment, growth, and reproduction of N. dubius in the selected mice. 相似文献
7.
The generation of protective immunity by various stages in the life-cycle of Strongyloides ratti and the phases against which resistance is directed has been examined in murine strongyloidiasis. Mice were exposed to natural, complete infections, were treated with thiabendazole (which largely resembles the natural infection), were treated with cambendazole (which restricts infection to the larval stage), or infected directly by oral transfer of adult worms. Mice that were infected with infective larvae alone did not become resistant to infective larvae or the complete infection but were resistant to adult worms implanted directly into the gut. Mice exposed to adult worms alone were resistant to natural infections and adults worms implanted directly but were not resistant to infective larvae. On the other hand, mice that had received prior natural infections showed evidence of resistance to infective larvae, adult worms, and natural, complete infections. It is concluded that there is immunological cross-reactivity between infective larvae and adult worms but that under certain circumstances the infective larvae are able to evade the host's protective immune response. 相似文献
8.
V Chaicumpa C R Jenkin D Rowley 《The Australian journal of experimental biology and medical science》1976,54(3):245-252
The results given below show that, whilst it is not possible to transfer immunity to infections with Nematospiroides dubius passively with serum from immune mice to normal adult mice, young born to immune females are resistant to this infection. The immunity is dependent on an intake of immunoglobulin via the milk for a period longer than 24 h. The passive transfer of immunity from immune mothers to neonatal mice does not appear to be dependent on a specific class of immunoglobulins. 相似文献
9.
J H Adams I J East F Monroy E A Washington C Dobson 《The Journal of parasitology》1987,73(6):1164-1168
Antigens were identified from Nematospiroides dubius recovered from outbred Quackenbush mice between 4 and 10 days postinoculation (PI). Parasite surface proteins were radioiodinated and extracts of the whole worms were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to nitrocellulose, and reacted with normal and immune mouse sera followed by an avidin-biotin-peroxidase assay. Antigens ranged between 250,000 and 20,000 molecular weight (MW). A major surface antigen, 60,000 MW, which appeared to be a complex of different antigens, and a 250,000-MW internal antigen were found on fourth-stage (L4) and fifth-stage (L5) larvae 5-10 days PI but not earlier. A group of minor surface antigens (24,000-30,000 MW) were also expressed as larvae molted from L4 to L5, 6 and 7 days PI, but they differed from antigens of similar MW expressed by adult worms. An antigen, 45,000 MW, was detected in worms 5-10 days PI, but it was only expressed on the surface of L5 worms 9 and 10 days PI. We suggest that the antigen(s) common to adults and larvae may account for protective immunity. 相似文献
10.
S J Prowse G F Mitchell P L Ey C R Jenkin 《The Australian journal of experimental biology and medical science》1978,56(5):561-570
BALB/c-nu/nu mice and their intact nu/+ littermates are equally susceptible to infection with third-stage larvae of Nematospiroides dubius. Unlike their heterozygous littermates, however, the nu/nu mice are unable to form ganulomata in the intestinal wall and become only partially resistant to rechallenge. Following two or more infections, nu/nu mice maintain a high burden of adult intestinal worms, whereas worms are lost from immune nu/+ mice. Studies in T cell-injected nu/nu mice suggest that a full complement of T cells is needed to develop maximum resistance against the infective third-stage larvae and to expel adult worms. Measurement of serum immunoglobulin levels indicate that infected nu/+ mice have very high levels of IgG1 whereas the levels of IgG2a are reduced. In infected T cell-injected nu/nu mice, IgG1 levels increase with the number of T cells injected, whereas IgG2a levels are variable but always higher than in infected nu/+ mice. 相似文献
11.
H-2 congenic strains expressing resistant (H-2q, H-2f) or susceptible (H-2k) haplotypes were compared for their ability to resist challenge infection with N. dubius following a 6- or 14-day ivermectin-abbreviated immunizing infection. B10.BR mice (H-2k) were considerably more resistant to infection when the priming interval was shortened from 14 to 6 days. B10.Q (H-2q) and B10.M (H-2f) mice resisted challenge regardless of which immunization regimen was used. The influence of parasite numbers on the response to challenge was studied by comparing infections in resistant DBA/1 (H-2q) and susceptible CBA/J (H-2k) mice that differ at both H-2 and non-H-2 genes. DBA/1 mice, immunized with 50 or 150 L3 of N. dubius for 14 days, resisted challenge, whereas mice receiving 300 worms did not. In contrast, CBA/J mice failed to resist challenge at all priming doses tested. When the immunizing infection was shortened from 14 to 6 days, DBA/1 mice resisted challenge regardless of priming dose and CBA/J mice resisted challenge only when the highest dose of 300 worms was used for priming. The data suggest that susceptible strains of mice may be preferentially immunosuppressed, particularly at low infective doses, and that suppression is associated with adult worms present in the lumen of the small intestine. 相似文献
12.
S O Alghali 《Journal of helminthology》1986,60(3):219-233
The dynamics of secondary infections with Hymenolepis citelli in mice are described. A primary infection of one and six cysticercoids for 21 days sensitized CFLP male mice against homologous challenge infections. Acquired resistance was manifested mainly as stunting/destrobilation of secondary worms. The severity of stunting depended on the intensity of the primary infection. Secondary worms were not expelled more rapidly than primary worms but the protective response retards growth early in challenge infections. Sensitization of mice for seven days with six or 24 cysticercoids did not confer a measurable protective response, whereas priming by the same regime for 21 days induced a significant protective response. Acquired resistance to challenge waned with time in the absence of the primary worms. The growth and survival of a six-cysticercoid primary infection was enhanced by the administration of the immunosuppressant drug cortisone acetate. Worms from cortisone-treated mice were heavier than those from untreated controls. Acquired resistance to homologous challenge was also partially ablated in cortisone-treated mice. It is suggested that rejection of primary infections and stunting/destrobilation of secondary worms may be immunologically mediated. 相似文献
13.
The pattern of peripheral blood leucocyte changes in mice infected with Nematospiroides dubius 总被引:2,自引:0,他引:2
Experiments were carried out to define the haematological changes taking place during the first six weeks of a primary infection with Nematospiroides dubius. The general pattern of changes was observed to comprise a rapid increase in circulating leucocytes (4 to 5-fold increase) which consisted of a neutropl a, lymphocytosis, monocytosis and an eosinophilia. However, in strong responder NIH mice leucocyte counts returned to normal more rapidly than in other strains (by day 28). In contrast, in weak responder C57BL/10 mice the leucocyte counts whilst falling significantly relative to day 7 did not return to normal within the experimental period. Mice infected with irradiated larvae did not experience as high a leucocytosis as did mice given an identical number of normal larvae. The peak lymphocytosis, neutrophilia and monocytosis were all lower. The removal of adult worms from infected animals by treatment with pyrantel on days 9, 11, 13 and 16, also significantly altered the pattern of leucocytosis. The neutrophilia which was evident on day 7 returned rapidly to normal, whereas in mice which had retained their worms a peak neutrophilia was observed on day 14. These haematological changes were discussed and related to the failure of host-protective immunity to operate effectively during the early stages of a primary infection with N. dubius. 相似文献
14.
Nematospiroides dubius: influence of adjuvants on immunity in mice vaccinated with antigens isolated by affinity chromatography from adult worms 总被引:1,自引:0,他引:1
Five adjuvants were examined for their ability to potentiate the immune response of mice to soluble antigens from adult Nematospiroides dubius prepared by affinity chromatography against antibodies from repeatedly infected mice as ligands (IMIgAg). Immunized mice were better protected against N. dubius by IMIgAg injected intraperitoneally with either pertussigen (75%) or aluminium hydroxide (Alum) (67%) as adjuvants than with Freud's complete (54%) or incomplete adjuvants (31%). Protection was correlated with elevated specific antibody values and with cellular responses. Quil A was toxic to recipient mice at the concentration used. Alum may be a more practical adjuvant than pertussigen, which may activate protective immunity only in specific recipient genotypes and oil-based adjuvants which appear to be less efficient, to vaccinate mice with soluble parasite antigens. 相似文献
15.
Dobson C. & Owen M. E. 1978. Effect of host sex on passive immunity in mice infected with Nematospiroides dubius. International Journal for Parasitology8: 359–364. Female C3H but not Quackenbush (Q) mice harboured fewer Nematospiroides dubius than male C3H and Q mice. Both strains lost worms 21 days after infection. C3H and Q mice became progressively immune to infection following 4 sequential doses of N. dubius larvae and showed a sex resistance to infection. Hypothymic nu/nu CBA Balb/c mice did not show these effects on N. dubius infection. The reciprocal transfer of male and female immune mesenteric lymph node cells (IMLNC) to syngeneic male and female recipients showed that the female environment enhanced the protective qualities of both male and female IMLNC but the male environment suppressed these effects. Gonadectomized male and female recipients of male and female IMLNC had levels of infection similar to the entire female recipients. Serum from immune female donor mice protected both male and female recipients better than immune serum from male donors, but female mice in each treatment group were better protected than male mice. Immune serum transferred greater levels of protection then IMLNC to recipient mice against N. dubius infections. These data are consistent with the conclusion that the male environment suppresses lymphocyte activity and the production of protective antibodies and additionally may depress the effectiveness of sensitized lymphocytes and antibodies in ejecting N. dubius. On the other hand the female environment does not appear to adversely affect the mobilization of the protective immune response and may enhance immune effector mechanisms in protecting mice against N. dubius infections. 相似文献
16.
Observations on the gross changes in the secondary lymphoid organs of mice infected with Nematospiroides dubius 总被引:2,自引:0,他引:2
Gross changes in the size of the secondary lymphoid organs were studied during infection with the nematode parasite Nematospiroides dubius. In the strong responder NIH strain, the wet weight of the mesenteric lymph nodes (MLN) increased rapidly following infection with 400 larvae to peak on day 28 at approximately three times the resting weight. Enlargement of the spleens was also marked but regression to normal size took place when the MLN had achieved maximum size. In contrast in C57BL/10 mice, a slow responder strain, the enlargement of the MLN following infection was relatively slow, and there was no evidence of the regression of the spleen, once maximum enlargement had been achieved. When adult worms were removed by anthelmintic, the enlarged MLN and spleens returned rapidly to normal size. However, in mice infected with irradiated larvae (25 krad) the MLN stayed enlarged, despite the absence of adult worms but the spleens of these mice returned to normal size fairly rapidly. It was suggested that irradiated worms survive, perhaps as arrested larvae in the intestinal tissue, for a fairly long time, thereby providing a continual stimulus for the MLN. 相似文献
17.
Adoptive immunization of A/Tru mice with splenic B cells or T cells from syngeneic donors with a primary, nonvirulent, Plasmodium yoelii (17X) infection confers on these recipients the capacity to resist a challenge infection with a virulent strain (YM) of P. yoelii. Unfractionated spleen cells as well as spleen cells enriched for T or B cells capable of transferring protective immunity were detected as early as Day 7 of the primary nonvirulent infection, and reached peak levels on Day 14. Spleen cells that were harvested from donor animals after resolution of the immunizing infection [on Days 21 or 28] were incapable of transferring protective immunity. The capacity of 7-day immune spleen cells to transfer immunity could be abolished by pretreatment with mitomycin C. In addition, it was found that immunocompetent recipient mice were required for successful adoptive immunization, since thymectomized, irradiated, bone marrow reconstituted mice infused with immune spleen cells failed to survive lethal challenge infections. 相似文献
18.
Experiments were conducted to examine adult worm burdens, fecal egg output, and in vitro fecundity of Nematospiroides dubius in resistant LAF1 and susceptible CBA mice 12, 15, 18, and 21 days following primary and challenge infections. A strong correlation was obtained on the number of eggs produced by worms cultured in vitro and the egg production as assessed by fecal egg count. Worm counts, fecal egg counts, and in vitro fecundity were similar on all days studied following a primary infection in both mouse strains. However, after challenge infection, LAF1 mice showed lower worm burdens, fecal egg output, and in vitro egg production when compared to CBA mice. Although the egg production of surviving female worms from immune LAF1 mice was decreased, it never fell below a threshold of 100 eggs/day. The reduced fecundity may be a manifestation of a general anti-worm response rather than responses directed specifically at worm reproduction. 相似文献
19.
Inbred strains of mice differ in their susceptibility and resistance to challenge infections with Nematospiroides dubius. In our studies, F1 hybrid mice from resistant SJL and susceptible CBA parents were resistant to N. dubius challenge infections. Only 22% of backcrosses to SJL were susceptible while backcrosses to CBA had a wide range of susceptibility. Male mice were more susceptible than female mice. In another experiment, inbred strains of mice were compared in their ability to resist N. dubius challenge infection: SJL and A.SW (H-2s) mice became resistant after one immunizing infection, A, A/He (both H-2a), as well as BALB/c and DBA/2 (both H-2d) mice became resistant after two immunizing infections, while C57BL/6 (H-2b), C3H/He, CBA, and AKR (H-2k) mice remained susceptible. The resistance to reinfections was characterized by reduction of worm burdens between Days 6 and 14 postinfection. It was concluded that (1) resistance to N. dubius challenge infections is inherited in a dominant fashion and that multiple genes may influence such response, which in turn might be modulated by the Y chromosome; (2) both MHC and non-MHC genes may influence, in conjunction with the number of exposures to parasite antigens, the resistance to challenge infections. 相似文献
20.
Holmes JC 《The Journal of parasitology》2002,88(3):434-9; discussion 433
Hymenolepis diminuta developing in the presence of Moniliformis dubius are lighter, shorter, have a lower average weight:length ratio, and are limited to the posterior part of the intra-intestinal range they occupy in single infections. M. dubius in the concurrent infections are lighter, possibly shorter, have a lower average weight:length ratio, and tend to attach further anterior than in single infections. No effects were noted on the percent recovery of either helminth. Highly significant regressions of the wet weight, length, and weight:length ratio of H. diminuta and M. dubius on the number of larvae administered in single infections have been demonstrated. The weight and length of the H. diminuta, and the weight and weight:length ratios of the M. dubius, from concurrent infections were significantly lower than values predicted for single-species infections with a comparable number of worms. In crowded single-species infections, the distribution of attachment points of the H. diminuta is extended to include the anterior three-fourths of the intestine; in concurrent infections the tapeworms are limited to the posterior part of this range. Under crowded conditions, the M. dubius extend their linear intestinal distribution only slightly, all of the acanthocephalans attaching in the anterior half of the small intestine; in concurrent infections the acanthocephalans tend to be limited to the anterior part of this range. The distributional limitations in concurrent infections and the similarities between the effects of concurrent infection and crowding suggest that similar mechanisms, i.e., competition, possibly for carbohydrate, may be involved in the production of the effects of concurrent infection. 相似文献