共查询到20条相似文献,搜索用时 31 毫秒
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Mager GM Ward RM Srinivasan R Jang SW Wrabetz L Svaren J 《The Journal of biological chemistry》2008,283(26):18187-18197
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Novel mutants of NAB corepressors enhance activation by Egr transactivators. 总被引:1,自引:0,他引:1 下载免费PDF全文
J Svaren B R Sevetson T Golda J J Stanton A H Swirnoff J Milbrandt 《The EMBO journal》1998,17(20):6010-6019
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Egr1 regulates the coordinated expression of numerous EGF receptor target genes as identified by ChIP-on-chip 总被引:1,自引:1,他引:0
Shilpi Arora Yipeng Wang Zhenyu Jia Saynur Vardar-Sengul Ayla Munawar Kutbuddin S Doctor Michael Birrer Michael McClelland Eileen Adamson Dan Mercola 《Genome biology》2008,9(11):R166
Background
UV irradiation activates the epidermal growth factor receptor, induces Egr1 expression and promotes apoptosis in a variety of cell types. We examined the hypothesis that Egr1 regulates genes that mediate this process by use of a chip-on-chip protocol in human tumorigenic prostate M12 cells. 相似文献13.
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Chasseigneaux S Dinc L Rose C Chabret C Coulpier F Topilko P Mauger G Allinquant B 《PloS one》2011,6(1):e16301
Background
sAPPα released after α secretase cleavage of Amyloid Precursor Protein (APP) has several functions including the stimulation of neurite outgrowth although detailed morphometric analysis has not been done. Two domains involved in this function have been described and are present in sAPPβ released at the first step of amyloid peptide cleavage, raising the possibility that sAPPβ could also stimulate neurite outgrowth. We investigated the morphological effects of sAPPα and sAPPβ on primary neurons and identified a key signaling event required for the changes observed.Methodology/Principal Findings
Final concentrations of 50 to 150 nM bacterial recombinant sAPPα or sAPPβ added to primary neuronal cultures after 1 day in vitro decreased cell adhesion 24 hours later and primary dendrite length 96 hours later. 150 nM sAPPα and sAPPβ induced a similar increase of axon outgrowth, although this increase was already significant at 100 nM sAPPα. These morphological changes induced by sAPPs were also observed when added to differentiated neurons at 5 days in vitro. Real time PCR and immunocytochemistry showed that sAPPα and sAPPβ stimulated Egr1 expression downstream of MAPK/ERK activation. Furthermore, in primary neurons from Egr1 −/− mice, sAPPs affected dendritic length but did not induce any increase of axon length.Conclusion/Significance
sAPPα and sAPPβ decrease cell adhesion and increase axon elongation. These morphological changes are similar to what has been observed in response to heparan sulfate. The sAPPα/sAPPβ stimulated increase in axon growth requires Egr1 signaling. These data suggest that sAPPβ is not deleterious per se. Since sAPPβ and sAPPα are present in the embryonic brain, these two APP metabolites might play a role in axon outgrowth during development and in response to brain damage. 相似文献16.
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