共查询到20条相似文献,搜索用时 31 毫秒
1.
Stéphanie Jaubert-Possamai Gaël Le Trionnaire Joël Bonhomme Georges K Christophides Claude Rispe Denis Tagu 《BMC biotechnology》2007,7(1):63
Background
RNA interference (RNAi) is a powerful method to inhibit gene expression in a sequence specific manner. 相似文献2.
Gene finding in novel genomes 总被引:1,自引:0,他引:1
Background
Computational gene prediction continues to be an important problem, especially for genomes with little experimental data. 相似文献4.
Russell L Legg Jessica R Tolman Cameron T Lovinger Edwin D Lephart Kenneth DR Setchell Merrill J Christensen 《Reproductive biology and endocrinology : RB&E》2008,6(1):57
Background
High dietary intake of selenium or soybean isoflavones reduces prostate cancer risk. These components each affect androgen-regulated gene expression. The objective of this work was to determine the combined effects of selenium and isoflavones on androgen-regulated gene expression in rat prostate. 相似文献5.
Daniel B Sloan Andrew J Alverson Helena Štorchová Jeffrey D Palmer Douglas R Taylor 《BMC evolutionary biology》2010,10(1):274
Background
Mitochondrial gene loss and functional transfer to the nucleus is an ongoing process in many lineages of plants, resulting in substantial variation across species in mitochondrial gene content. The Caryophyllaceae represents one lineage that has experienced a particularly high rate of mitochondrial gene loss relative to other angiosperms. 相似文献6.
Nélida Pohl Marilou P Sison-Mangus Emily N Yee Saif W Liswi Adriana D Briscoe 《BMC evolutionary biology》2009,9(1):99
Background
The increase in availability of genomic sequences for a wide range of organisms has revealed gene duplication to be a relatively common event. Encounters with duplicate gene copies have consequently become almost inevitable in the context of collecting gene sequences for inferring species trees. Here we examine the effect of incorporating duplicate gene copies evolving at different rates on tree reconstruction and time estimation of recent and deep divergences in butterflies. 相似文献7.
Larry R Karns PhD Anne Kisielewski BSc Kathryn M Gulding BSc Dan Theodorescu MD PhD 《BMC biotechnology》2001,1(1):11-12
Background
Rapid, robust and reversible induction of transgene expression would significantly facilitate cancer gene therapy as well as allow the in vivo functional study of newly discovered genes in tumor formation and progression. The popularity of the ecdysone inducible gene switch system has led us to investigate whether such a system can successfully regulate gene expression in a syngeneic tumor system in vivo. 相似文献8.
Background
Allopolyploid speciation requires rapid evolutionary reconciliation of two diverged genomes and gene regulatory networks. Here we describe global patterns of gene expression accompanying genomic merger and doubling in inter-specific crosses in the cotton genus (Gossypium L.). 相似文献9.
Background
The objectives of this study were to develop an easy and rapid method for measuring gene expression in a small number of cells by real-time PCR without RNA extraction and purification, and to use this method to determine more precisely IGF-I gene expression in the cumulus cells surrounding oocytes. 相似文献10.
Seon-Young Kim 《BMC bioinformatics》2009,10(1):147
Background
Few overlap between independently developed gene signatures and poor inter-study applicability of gene signatures are two of major concerns raised in the development of microarray-based prognostic gene signatures. One recent study suggested that thousands of samples are needed to generate a robust prognostic gene signature. 相似文献11.
Background
Recently duplicated genes are often subject to genomic rearrangements that can lead to the development of novel gene structures. Here we specifically investigated the effect of variations in internal tandem repeats (ITRs) on the gene structure of human paralogs located in segmental duplications. 相似文献12.
Andrew S Warren Jeremy Archuleta Wu-chun Feng João Carlos Setubal 《BMC bioinformatics》2010,11(1):131
Background
Protein-coding gene detection in prokaryotic genomes is considered a much simpler problem than in intron-containing eukaryotic genomes. However there have been reports that prokaryotic gene finder programs have problems with small genes (either over-predicting or under-predicting). Therefore the question arises as to whether current genome annotations have systematically missing, small genes. 相似文献13.
Sally-Anne Stephenson Stefan Slomka Evelyn L Douglas Peter J Hewett Jennifer E Hardingham 《BMC molecular biology》2001,2(1):15-9
Background
We have used commercially available cDNA arrays to identify EphB4 as a gene that is up-regulated in colon cancer tissue when compared with matched normal tissue from the same patient. 相似文献14.
Background
Many important evolutionary adaptations originate in the modification of gene regulatory circuits to produce new gene activity phenotypes. How do evolving populations sift through an astronomical number of circuits to find circuits with new adaptive phenotypes? The answer may often involve phenotypic plasticity. Phenotypic plasticity allows a genotype to produce different - alternative - phenotypes after non-genetic perturbations that include gene expression noise, environmental change, or epigenetic modification. 相似文献15.
Yuexing Zhang Yan Lu Hua Zhou Myounghee Lee Zhenqiu Liu Bret A Hassel Anne W Hamburger 《BMC cell biology》2008,9(1):69
Background
The ErbB3 binding protein-1 (Ebp1) belongs to a family of DNA/RNA binding proteins implicated in cell growth, apoptosis and differentiation. However, the physiological role of Ebp1 in the whole organism is not known. Therefore, we generated Ebp1-deficient mice carrying a gene trap insertion in intron 2 of the Ebp1 (pa2g4) gene. 相似文献16.
17.
Background
With the ever-increasing number of gene sequences in the public databases, generating and analyzing multiple sequence alignments becomes increasingly time consuming. Nevertheless it is a task performed on a regular basis by researchers in many labs. 相似文献18.
Alicia S Linford Heriberto Moreno Katelyn R Good Hanbang Zhang Upinder Singh William A Petri 《BMC microbiology》2009,9(1):38
Background
Entamoeba histolytica is an intestinal protozoan parasite of humans. The genome has been sequenced, but the study of individual gene products has been hampered by the lack of the ability to generate gene knockouts. We chose to test the use of RNA interference to knock down gene expression in Entamoeba histolytica. 相似文献19.
Barry?R?Zeeberg Joseph?Riss David?W?Kane Kimberly?J?Bussey Edward?Uchio W?Marston?Linehan J?Carl?Barrett John?N?Weinstein
Background
When processing microarray data sets, we recently noticed that some gene names were being changed inadvertently to non-gene names. 相似文献20.
Radim Cegan Gabriel AB Marais Hana Kubekova Nicolas Blavet Alex Widmer Boris Vyskot Jaroslav Doležel Jan Šafář Roman Hobza 《BMC plant biology》2010,10(1):180