共查询到18条相似文献,搜索用时 125 毫秒
1.
裙带菜假根中褐藻糖胶的提取 总被引:1,自引:0,他引:1
研究了温度、pH值、提取时间和水藻比及添加果胶酶和纤维素酶对采用乙醇沉淀法提取裙带菜假根中褐藻糖胶的影响。单因素和正交实验结果表明,80℃p、H 5、提取时间5 h下的褐藻糖胶得率最高,水藻比影响不明显。添加500 u/g果胶酶和纤维素酶可分别增加57%和46%。 相似文献
2.
羊栖菜中褐藻糖胶的组分分离及分析 总被引:4,自引:0,他引:4
为了确定羊栖菜褐藻糖胶中的活性组分 ,用分子量结合硫酸化程度的分级方法将其分成不同组分。经热水抽提的羊栖菜多糖 ,将去除褐藻胶、海带淀粉后剩余的褐藻糖胶经过DEAE—Sepharose离子交换色谱柱和Sepharose 4B凝胶层析柱被分成 5个褐藻糖胶组分。 5个组分中硫酸基和岩藻糖近似的分子数量之比分别为 1.863、0 .0 68、1.2 2 9、1.62 9,1.0 10 ;平均分子量分别为 2 .0× 10 4 、2 .2× 10 4 、8.2× 10 4 、2 1.4× 10 4 、32 .0× 10 4 ;百分含量分别为 8.6%、2 2 .9%、4 8.5 %、5 .7%、14.3%。 相似文献
3.
海带中褐藻糖胶的分级纯化与结构研究 总被引:15,自引:0,他引:15
4.
褐藻裙带菜色素—蛋白质复合物的分离与命名 总被引:1,自引:0,他引:1
以非离子去污剂癸基-N-甲基葡萄糖胺为增溶剂,采用聚丙烯酰胺凝胶电泳技术从褐藻裙带菜(Undaria pinnatifida Harv.)的类囊体膜上分离到8种色素-蛋白质复合物。根据其表观分子量、光谱特性和多肽分析结果,并以高等植物菠菜(Spinacia oleracea L.)为对照,按照Anderson命名系统,8种色素-蛋白质复合物分别命名为CPⅠ a、CPⅠ、CPa、LHC1、LHC2、 相似文献
5.
综述了褐藻糖胶结构方面的研究进展,以及褐藻糖胶的抗凝血活性和抗病毒活性与结构之间的关系。 相似文献
6.
羊栖菜褐藻糖胶抗凝血活性的研究 总被引:7,自引:2,他引:7
本文研究了羊栖菜褐藻糖胶的化学组成和抗凝血活性之间的关系。采用热水提取得羊栖菜粗多糖,CaCl2纯化得褐藻糖胶,DEAE Sepharose CL-6B柱层析与Sepharose CL-6B柱层析对褐藻糖胶进行分级,得到F1、F2、F31、F32和F33五个级分,均为岩藻糖、半乳糖和甘露糖等糖基组成的杂多糖,并含有硫酸酯和糖醛酸以及少量的蛋白质,相对分子质量范围2.5万~95万。采用活化部分凝血活酶时间(APTT)和凝血酶时间(TT)检测了这5个级分的抗凝血活性,结果显示,羊栖菜褐藻糖胶能显著延长APTT的凝血时间,而对TT的影响不明显。F1、F31和F32对APTT的影响比较显著,而F2、F33和羊栖菜粗多糖的影响较小。研究表明,羊栖菜褐藻糖胶主要是通过抑制内源凝血途径而达到抗凝血的效果,其抗凝血活性与褐藻糖胶的硫酸基含量成正相关,而与相对分子质量和糖醛酸含量无关。 相似文献
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研究了超声波辅助提取褐藻糖胶的新工艺,以“硫酸根+多糖”质量分数作为产品的主要理化指标,得到优化的提取工艺。硫酸根和多糖质量分数分别达到22.49%和36.97%。 相似文献
10.
胡乃华 《天然产物研究与开发》2022,(2)
褐藻糖胶也称为岩藻黄质、岩藻多糖、褐藻多糖,是一种硫化的多糖,常见于海参、海藻等褐藻纲及海藻类的植物中。研究发现,具有特定硫酸化结构的糖类结构域在生物功能中起着至关重要的作用。因此,对褐藻糖胶寡糖进行精细的结构表征,尤其是确定其硫酸化结构,对于理解褐藻糖胶的结构-功能关系至关重要。 相似文献
11.
Mika Takai Yoshiyuki Miyazaki Hirofumi Tachibana 《Bioscience, biotechnology, and biochemistry》2013,77(10):1743-1747
In this study, we revealed that a Mekabu (Udaria pinnantifida) extract enhanced immunoglobulin (Ig) production of mouse spleen lymphocytes. Furthermore, it was suggested that water-soluble and high molecular weight ingredients in the Mekabu extract have significant enhancing effect on Ig production. Therefore, fucoidan was estimated as the active component. 相似文献
12.
本文报告了用14C标记的光同化产物在裙带菜[Undariapinnatifida(Hary.)Sur]孢子体中的运转现象。观察到光同化产物约需20min才能从叶片表皮进入中肋的髓部。在自然条件下,光同化产物主要自叶片梢部经中肋向生长部运转,在叶片梢部和生长部之间存在明显的源一库关系。运转速度约为42~48cm/h。用组织放射自显影的方法证实了髓部的喇叭丝是主要的运转组织。向下运转的溶于酒精的光同化产物中,甘露醇占50%以上。还在光同化产物中观察到游离的谷氨酸、天门冬氨酸和丙氨酸。 相似文献
13.
Eight kinds of pigment-protein complexes were resolved from the thylakoid membrane of the brown alga (Undaria pinnatifida Harv.) by using non-ionic detergent decanoyl-N-methylglucamide and PAGE technique. According to the apparent molecular weights, spectra characteristics, polypeptide compositions and referring to the higher plant spinach, eight pigment-protein complexes were named under Anderson′s terminology system as CPⅠa, CPⅠ, CPa, LHC1, LHC2, LHC3, LHC4, LHC5. 相似文献
14.
The relative growth rate of young sporophytes of Undaria pinnatifida (Harvey) Suringar and Undaria undarioides (Yendo) Okamura was examined in order to understand the difference in distribution of these two species around the coast of Japan. The optimal temperature for growth of both species was similar at 20°C and the upper critical temperature for growth was also similar, at 27°C for U. pinnatifida and 26°C for U. undarioides. Therefore, the optimal and upper critical temperatures for growth of the young sporophytes are not the main factors determining the distribution of each species. Next, the lower critical temperatures for growth were examined. For the young sporophytes of U. pinnatifida, the lower limit was less than 5°C while for those of U. undarioides it was 15°C. Thus, the difference in the lower critical temperature for growth between the two species was approximately 10°C. During the period of young sporophyte growth in the field, the temperature at the mouth of Ise Bay, Japan, where U. pinnatifida occurs, ranges from 12.7°C in December to 13.1°C in April, with a minimum of 7.9°C in February. Our experiments indicate that young sporophytes are able to grow throughout this period. The temperature off Hamajima, Japan, where U. undarioides occurs, ranges from 19.1°C to 14.8°C during the same time period. Again, young sporophytes are able to growth throughout this period, although minimum winter temperatures are only just high enough for growth. These natural temperature ranges during the growth season of the sporophytes agree well with the experimentally determined temperature requirements for growth of each species. Therefore, the difference between the two species in the critical temperature required for growth of the young sporophytes, especially in the low temperature range, is one of the major factors determining the distribution pattern of each species. 相似文献
15.
A slow-release ammonium phosphate fertilizer coated with porous plastic was tested on Undaria pinnatifida (Harvey) Suringar as a possible solution to the nutrient deficiency in seawater that causes quality and yield deterioration in seaweed farming. The yield of U. pinnatifida within the fertilized area was 17–40% greater than that of the control area (unfertilized area). In addition, two harvests were possible per season and the quality of harvested U. pinnatifida was also better than that outside the fertilizer diffusion area. The released NH4-N did not increase the concentration of NH4-N outside the farming area. Therefore, this fertilizer increased yield and improved quality without causing water pollution. 相似文献
16.
褐藻裙带菜孢子叶粗提物体外抗单纯疱疹病毒Ⅱ型活性研究 总被引:5,自引:0,他引:5
从药物对细胞的保护、对HSV-2增殖的影响及对HSV-2感染细胞的综合作用三个方面研究不同稀释度的裙带菜孢子叶粗提物抑制单纯疱疹病毒Ⅱ型对Vero细胞的感染作用,细胞病变效应法(Cytopathogenic effect,CPE)观察和MTT法测定裙带菜多糖抗HSV-2活性,结果表明裙带菜多糖能明显抑制HSV-2对Vero细胞的致病变作用,使细胞存活率升高,其水提醇沉法所得裙带菜多糖的IC50为6.49μg/mL,并初步推测其抗HSV-2活性是作用在HSV-2和受体结合,侵入Vero细胞阶段,为筛选新型抗病毒药物、研究海藻多糖抗HSV-2活性机理及优化裙带菜孢子叶的提取工艺提供参考依据。 相似文献
17.
Undaria cultivation on a commercial scale began in China only in the last decade. Today, Undaria pinnatifida is the main species
under cultivation concentrated in two provinces, Liaoning and Shandong. The annual production in the early nineties was 8000-13
000 tons dry weight, which is two or three times the pre-1980 figures. The raft cultivation method maintaining the alga at
the desired depths generally ensures the light saturated rate of photosynthesis on clear days, and enhances production. Under
the cultivated condition, the calculated annual primary productivity of this alga is 160 gC m−2 y−1.
Translocation of 14C-labelled photoassimilates in rapidly growing sporophyte of Undaria pinnatifida was studied in the open sea. Samples from
different parts of the blade with counterparts exposed to tracer (NaH14CO3) showed that the translocation that occurred mainly from the tip of the blade to the growing region had obvious source-sink
relationship. It took 20 minutes to translocate the labelled photoassimilates from the epidermis, via cortex, to the medulla
of the midrib, where rates of translocation averaging 42–48 cm h−1 were observed in the open sea.
Production experiments of tip-cutting of the blades showed an increased production of 9%.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
18.
We sequenced mitochondrial 23S ribosomal DNA (rDNA) from two Undaria species and compared them with homologous sequences from related brown algae. The 23S rDNA sequences from Undaria pinnatifida (Harvey) Suringar were 2707 bp in length, of which 1290–1292 sites were assigned to stem regions and the rest to loop regions in the rRNA secondary structure model. In Undaria undarioides (Yendo) Okamura, the sequences were 2708–2709 bp in length and 1290 sites were assigned to stems. There were three sequence types among the seven U. pinnatifida individuals examined and two among the four U. undarioides individuals. A maximum parsimony analysis of these sequence data with those from related brown algal species resolved a robust monphyly of each of the two Undaria species, suggesting the utilities of 23S rDNA for phylogeny at species or intraspecific level in brown algae. Accounting for variations among sequences parsimoniously, sequence changes were sorted into 423 events, of which 165 were mapped to stems of the secondary structure. 相似文献