共查询到20条相似文献,搜索用时 15 毫秒
1.
Lectins are carbohydrate-binding proteins that are ubiquitous in nature. Their ability to specifically bind carbohydrates
has been used as a means of purification mainly through affinity chromatography techniques. Plant lectins are one of the most
thoroughly studied class of lectins, however, details of theirin situ function remains elusive. Recent advances in recombinant DNA techniques have been used in several laboratories to study the
function of these lectins by heterologous over-expression. The larger subunit of theDolichos biflorus seed lectin was described by Chao et al. in 1994 and purification through affinity chromatography techniques was described.
Here we report on a new method for the purification of this recombinant protein with techniques that are not dependent on
the ability of the lectin to bind sugars. This method may have uses in the purification of mutant proteins that may not bind
carbohydrates. Characterization of the purified protein by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)
and matrix-assisted laser desorption ionization (MALDI) mass spectroscopy shows that the lectin is over 99% pure with a molecular
weight of 27,090±16.17 Da, and hemagglutination assays confirm that the lectin retains its biological activity. 相似文献
2.
Roberto Colangeli Anna Heijbel Alan M Williams Claudia Manca Joena Chan Konstantin Lyashchenko Maria Laura Gennaro 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1998,714(2):32
Previous work has shown that the study of host immune responses against Mycobacterium tuberculosis, the causative agent of tuberculosis, requires the availability of multiple mycobacterial antigens. Since purification of protein from M. tuberculosis cells is extremely cumbersome, we developed a protocol for purifying milligram amounts of ten recombinant antigens of M. tuberculosis from E. coli cells. Purified proteins were immunologically active and free of contaminants that confound interpretation of cell-based immunological assays. The method utilizes a three-step purification protocol consisting of immobilized metal-chelate affinity chromatography, size exclusion chromatography and anion-exchange chromatography. The first two chromatographic steps yielded recombinant protein free of protein contaminants, while the third step (anion-exchange chromatography) efficiently removed E. coli lipopolysaccharide, a potent polyclonal activator of lymphoid cells. The recombinant proteins were immunologically indistinguishable from their native (i.e., purified from M. tuberculosis) counterparts. Thus the method provides a way to utilize recombinant proteins for immunological analyses that require highly purified antigens. 相似文献
3.
A lectin was purified from fruit bodies of the milk mushroom Lactarius pergamenus (Fr.) Fr. by a combination of ethanol precipitation, affinity chromatography on copolymer of polyvinyl alcohol and human
blood B-group-specific sub-stance, and ion-exchange chromatography on DEAE-Toyopearl. The lectin yield was 3 mg/kg of fresh
mushrooms. Considerable loss of primary activity was observed during its purification, which, presumably, could be explained
by disin-tegration of the lectin molecule, which consisted of six subunits, first to two molecules of three subunits, and
then to individual subunits. There was a reverse tendency to aggregation during concentration of lectin solutions. Similar
processes can take place in nature because of considerable individual variations of the lectin activity during growth of mushroom
fruit bodies. The lectin weakly interacts with DGalNAc, while DGalβ1-3DGalNAc and DGalβ1-3DGlcNAc are the most probable candidates
for ligands, with which the L. pergamenus lectin interacts at disaccharides level. The purified lectin may find application in histochemical research. 相似文献
4.
Purification and functional characterization of lectin with phenoloxidase activity from the hemolymph of cockroach,Periplaneta americana
下载免费PDF全文
![点击此处可从《Archives of insect biochemistry and physiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Ganesh Arumugam Bhuvaragavan Sreeramulu Ramaraj Paulchamy Sakthivel Thangavel Janarthanan Sundaram 《Archives of insect biochemistry and physiology》2017,95(2)
Lectins also identified as hemagglutinins are multivalent proteins and on account of their fine sugar‐binding specificity play an important role in immune system of invertebrates. The present study was carried out on the hemolymph lectin of cockroach, Periplaneta americana with appropriate screening and purification to understand its molecular as well as functional nature. The lectin from the hemolymph was purified using ion‐exchange chromatography. The approximate molecular weight of purified lectin was 340 kDa as determined by FPLC analysis. Rabbit erythrocytes were highly agglutinated with purified lectin from the hemolymph of P. americana. The hemagglutination activity (HA) of lectin was specifically inhibited by fucose. Glycoproteins also inhibited the HA activity of lectin. The amino acid sequences of the purified lectin revealed homology with amino acid sequences of allergen proteins from P. americana. Purified lectin showed the highest phenoloxidase activity against dopamine. The activators such as exogenous proteases and LPS from Escherichia coli and Salmonella minnesota significantly enhanced the PO activity of the purified lectin. Besides, the presence of copper and hemocyanin conserved domain in the purified lectin provided a new facet that insects belonging to the ancient clade such as cockroaches retained some traces of evolutionary resemblance in possessing lectin of ancient origin. 相似文献
5.
《Bioscience, biotechnology, and biochemistry》2013,77(8):1237-1242
A lectin was isolated from hemolymph of the Southeast Asian horseshoe crab Tachypleus gigas by using glycophorin HA affinity chromatography and Sephacryl S-300 gel filtration. The purified lectin had a molecular mass of approximately 396 kDa and was composed of 13 identical subunits with molecular masses of 31 kDa. The serological specificity of the purified lectin was specifically inhibited by sialic acids sialoglycoproteins, but not by neutral sugars, hexosamines, N-acetylhexosamines, or asialoglycoproteins. Although the N-terminal amino acid sequence of the lectin from T. gigas was identical to that from American horseshoe crab (liphemin) by the same purification method and cross reacted with the anti-liphemin serum, the calcium concentration of hemagglutinating activity of the purified lectin showed a smaller optimal concentration than that of liphemin. 相似文献
6.
Three mannose-specific lectins exhibiting considerable similarities in NH2-terminal amino acid sequence were isolated from leaves of the Chinese daffodil Narcissus tazetta (Family Amaryllidaceae). The purification protocol involved extraction with an aqueous buffer, anion exchange chromatography on DEAE-cellulose using stepwise elution with increasing salt concentrations, affinity chromatography on mannose-agarose, and FPLC-gel filtration on Superose 12. From the peak unadsorbed on DEAE-cellulose, and two peaks adsorbed on the ion exchanger and eluted respectively with 0.2 M Tris-HCl buffer and 0.5 M NaCl, were prepared fractions which yielded isolectins 1, 2, and 3 after adsorption on mannose-agarose and FPLC-gel filtraton. All three isolectins were homodimers with a molecular weight of 26 kDa. The lectin unadsorbed on DEAE-cellulose had the lowest, while the most strongly adsorbed lectin had the highest hemagglutinating activity. 相似文献
7.
Evidence for the occurrence of calmodulin in the yeasts Candida albicans and Saccharomyces cerevisiae 总被引:5,自引:0,他引:5
The lectin extracted from Vicia graminea seeds has been purified by conventional techniques but such procedures did not give a satisfactory yield. We describe a new purification which involves 3 steps after obtention of the crude extract. The first step is based on affinity chromatography on con A—Sepharose. Further purification steps were performed on DEAE-Sephacel chromatography and ultrogel AcA44 gel filtration. The homogeneity of the lectin was demonstrated by polyacrylamide gel electrophoresis. Purification of the lectin by this new method was less time consuming, the yield was higher and the specific activity increased. 相似文献
8.
Two lectin fractions (So20 w = 6,8 and 4,9 S) were purified from Ricinus communis seeds. The purification was carried out in four steps : ammonium sulfate fractionation, affinity chromatography on Sepharose 4 B, gel filtration on Sephadex G 150 and chromatography on CM cellulose. The purified lectins were glycoproteins whose chemical composition was determined. Amino terminal analysis of the two fractions revealed glycine and serine. Polyacrylamide gel electrophoresis of the higher molecular weight fraction allowed the separation of several components with different affinity for PAS staining. 相似文献
9.
Holger Husi Malcolm D. Walkinshaw 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2001,755(1-2)
Human factor X has been purified to homogeneity by hydrophobic interaction chromatography on phenyl-sepharose. The coagulation protein did not interact with the resin in the presence of 2–3 M NaCl whereas contaminants were retained. This single purification step, in conjunction with classical purification strategies, is a powerful tool in generating high purity factor X and is based on resins which are readily available. 相似文献
10.
We describe here a simple, general procedure for the purification of a variety of lectins, and for the preparation of lectin-ferritin conjugates of defined molar composition and binding properties to be used as probes for cell surface saccharides. The technique uses a “universal” affinity column for lectins and their conjugates, which consists of hog sulfated gastric mucin glycopeptides covalently coupled to agarose. The procedure involes: (a) purification of lectins by chromatography of aqueous extracts of seeds or other lectin-containing fluids over the affinity column, followed by desorption of the desired lectin with its hapten suge; (b) iodination of the lectin to serve as a marker during subsequent steps; (c) conjugation of lectin to ferritin with glutaraldehyde; (d) collection of active lectin-ferritin conjugates by affinity chromatography; and (e) separation of monomeric lectin-ferritin conjugates from larger aggregates and unconjugated lectin by gel chromatography. Based on radioactivity and absorbancy at 310 nm for lectin and ferritin, respectively, the conjugates consist of one to two molecules of lectin per ferrritin molecule. Binding studies of native lectins and their ferritin conjugates to dispersed pancreatic acinar cells showed that the conjugation procedure does not significantly alter either the affinity constant of the lectin for its receptor on the cell surface or the number of sites detected. 相似文献
11.
免疫亲和层析法纯化棕尾别麻蝇(Sarcophagaperegrina)幼虫血淋巴凝集素 总被引:1,自引:0,他引:1
报道了利用免疫亲和层析法纯化棕尾别麻蝇幼虫血淋巴凝集素的结果.哺乳动物红细胞能够特异地吸附凝集素.用兔红细胞与麻蝇幼虫血淋巴凝集素形成的复合体免疫供血家兔,得到麻蝇幼虫血淋巴凝集素的抗体.再利用抗体制备亲和吸附柱,通过免疫亲和层析一次性纯化了麻蝇幼虫血淋巴凝集素. S D S P A G E结果显示,该凝集素的分子量约为73 k D.这一结果,与用对麻蝇幼虫血淋巴凝集素有抑制作用的糖蛋白—胎球蛋白和甲状腺球蛋白为配基,亲和层析纯化的结果完全相同,表明用这种免疫亲和层析法纯化凝集素是可行的.为不清楚专一性识别糖或专一性识别糖不典型,难于用普通亲和层析纯化的凝集素,提供了一种有效的纯化方法. 相似文献
12.
One-step partial purification of Solanum tuberosum tuber lectin using magnetic chitosan particles 总被引:2,自引:0,他引:2
A one-step procedure for a partial purification of Solanum tuberosum tuber lectin has been developed. Lectin from tuber extract or from potato wastewater was adsorbed to magnetic chitosan particles and eluted with low pH buffer. The specific activity of separated lectin increased 13 times during the purification process and the recovery was 50%. 相似文献
13.
Juan Lin Jianhong Yao Xuanwei Zhou Xiaofen Sun Kexuan Tang 《Molecular biotechnology》2003,25(3):215-221
Pinellia ternata agglutinin (PTA) from the tubers of P. ternata is a monocot mannose-binding lectin that catalytically agglutinated rabbit erythrocytes. The potential effect of PTA has
gained considerable interest in recent years owing to clinical use of native PTA as the preparation against cancer and for
plant protection against insect pests. Here we report a successful strategy to allow high-level expression of PTA as inclusion
bodies in Escherichia coli M15. Purification of refolded recombinant protein from solubilized inclusion bodies by Ni-NTA agarose affinity chromatography
yielded biological activity recombinant PTA (final yield of about 10 mg/L). The recombinant PTA agglutinated rabbit erythrocytes
to a dilution similar to that determined for “native” lectin purified from P. ternata. The expression and purification system makes it possible to obtain sufficient quantities of biologically active and homogenous
recombinant PTA sufficient to carry out advanced clinical trials. This is the first report on the large-scale expression and
purification of biologically active recombinant PTA from E. coli. 相似文献
14.
Lectins have been used in glycoprotein purification, oligosaccharide analysis, and in cell‐selection processes. Here, we utilize lectins in a rational attempt to select a subpopulation of insect cells (Estigmene acrea, EAA) with more complete glycosylation capacity by selecting cells that display more complex‐type cell‐surface oligosaccharides than the general population of cells. A lectin (ECA) from Erythrina cristagalli, specific for galactose β(1‐4)N‐acetylglucosamine, was found to be useful in recognizing a small subpopulation of Sf‐21 and EAA cells. Cell selections were performed by lectin affinity chromatography and by selective agglutination. Analysis by lectin blots of cell lysates and a quantitative agglutination assay did not reveal significant differences in regard to the level of complex glycosylation between the negatively and positively selected subpopulations of EAA cells. Statistically significant differences in binding the fluorescently labeled lectin, ECA‐TRITC were observed even 30 passages post‐selection between EAA subpopulations that were negatively and positively selected by lectin affinity chromatography. There were no differences in the two subpopulations in the ECA quantitative agglutination assay. Thus, the hypothesis that a subpopulation differing in glycosylation capacity exists and that such a subpopulation can be identified by the character of cell‐surface oligosaccharides is plausible. However, these differences appear to be too small to be of practical use. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 64: 616–619, 1999. 相似文献
15.
Min Gui Jung Key Pyoung Lee Han-Gu Choi Sung-Ho Kang Tatyana A. Klochkova Jong Won Han Gwang Hoon Kim 《Journal of applied phycology》2010,22(6):793-802
Bryohealin is a lectin involved in the wound-healing process of the marine green alga Bryopsis plumosa. In the previous purification study, it has been shown that lectin was composed of two identical subunits of 27 kDa, cross-linked
by disulfide bond, and showed binding specificity to N-acetyl-d-glucosamine and N-acetyl-d-galactosamine (GlcNAc and GalNAc, respectively). To determine if the lectin recognize the two different sugars at the same
binding domain, the carbohydrate binding sites of Bryohealin was analyzed using chromatography and chemical modification methods.
Results showed that the same binding site of the lectin was responsible for the recognition of two sugars, GalNAc as well
as GlcNAc. Chemical modification studies showed that hemagglutinating activities of Bryohealin were not affected by modification
of histidine, tryptophan, aspartic acid, and glutamic acid. When arginine residues were modified with 1,2-cyclohexanedione,
the activity of Bryohealin rapidly decreased. The sugar binding sites remained intact when the lectin was treated with inhibitory
sugars (0.2 M GalNAc and/or GlcNAc) prior to 1,2-cyclohexanedione treatment. The sugar binding domain of Bryohealin was predicted
from the MALDI-TOF analysis and the full cDNA sequence of the lectin gene. 相似文献
16.
Non-specifically bound nucleic acid contaminants are an unwanted feature of recombinant RNA-binding proteins purified from Escherichia coli (E. coli). Removal of these contaminants represents an important step for the proteins’ application in several biological assays and structural studies. The method described in this paper is a one-step protocol which is effective at removing tightly bound nucleic acids from overexpressed tagged HIV-1 Rev in E. coli. We combined affinity chromatography under denaturing conditions with subsequent on-column refolding, to prevent self-association of Rev while removing the nucleic acid contaminants from the end product. We compare this purification method with an established, multi-step protocol involving precipitation with polyethyleneimine (PEI). As our tailored protocol requires only one-step to simultaneously purify tagged proteins and eliminate bound cellular RNA and DNA, it represents a substantial advantage in time, effort, and expense. 相似文献
17.
18.
Microcystis viridis lectin (MVL), a sugar-binding protein originally isolated from freshwater blue-green algae Microcystis viridis, has been reported to have potent anti-HIV activity. In this paper, we described the expression and purification of recombinant-MVL
(R-MVL) gene in E. coli. The results demonstrated that the R-MVL in shake flask cultures was primarily expressed either in the form of inclusion
bodies at 37°C or in the soluble fraction at 23 °C. Secondly, a one-step purification based on nickel-affinity chromatography
was employed and 15 mg of highly purified (>95%) R-MVL from 1 l of cell cultures was yielded. The purified R-MVL was then
subjected to MALDI-TOF–MS analysis for protein identification. In conclusion, for the first time, the R-MVL was successfully
cloned and expressed in E. coli, which is useful for further study and large-scale cost-effective production of MVL protein. 相似文献
19.
A lectin has been isolated from rhizomes of ground elder (Aegopodium podagraria) using a combination of affinity chromatography on erythrocyte membrane proteins immobilized on cross-linked agarose and hydroxyapatite, and ion-exchange chromatography. The molecular structure of the lectin was determined by gelfiltration, sucrose density-gradient centrifugation and gel electrophoresis under denaturing conditions. It has an unusually high Mr (about 480000) and is most probably an octamer composed of two distinct types of subunits with slightly different Mr (about 60000). Hapten inhibition assays indicated that the Aegopodium lectin is preferentially inhibited by N-acetylgalactosamine. Nevertheless, it does not agglutinate preferentially blood-group-A erythrocytes. The ground-elder lectin is a typical non-seed lectin, which occurs virtually exclusively in the underground rhizomes. In this organ it is an abundant protein as it represents up to 5% of the total protein content. The lectin content of the rhizome tissue varies strongly according to its particular location along the organ. In addition, the lectin content changes dramatically as a function of the seasons. The ground-elder lectin differs from all other plant lectins by its unusually high molecular weight. In addition, it is the first lectin to be isolated from a species of the family Apiaceae.Abbreviations APA
Aegopodium podagraria agglutinin
- PBS
phosphate-buffered saline
- SDS-PAGE
sodium dodecyl sulfate polyacrylamide gel electrophoresis 相似文献
20.
Atsuya Ishiwari Tomoko Akutsu Hiroshi Ikegaya Koichi Sakurada 《Journal of plant biochemistry and biotechnology.》2010,19(2):243-245
Chaenomeles sinensis lectin (CSL) was isolated from Chinese quince fruit by affinity chromatography. The molecular weight of native CSL was estimated to be 16 kD by gel filtration chromatography. This lectin was found to contain approximately 57% carbohydrates. The molecular weight of deglycosylated CSL was estimated to be 3.6 kD by tricine-SIDS-PAGE under reduced conditions. Our results suggest that CSL may be a homodimer. The hemagglutinating activity of CSL was inhibited by N-acetyllactosamine and chicken ovalbumin, and it was drastically decreased at pH levels of 9.0 or greater. CSL may be a useful tool for the purification of glycoconjugates. 相似文献