On the stomata ofSalvinia herzogii (Salviniaceae,Pteridophyta)

The structure of the stomata of the floating leaves ofSalvinia herzogii has been analyzed with light and scanning electron microscopy. Peculiar extensions (cuticle?) of the outer and inner tangential sides of the walls of the guard-cells are described. Both extensions, partially joined at their ends, are encircling a pore. This structure is used to propose an alternative explanation for the risk of the occlusion of the ostioles, different from that given byHaberlandt. The conductivity of these stomata was checked with a porometer and the ostioles proved to be permanently open. Their type of stomatogenesis is discussed. The stomata ofSalvinia are considered to be vestigial structures and their sunken position is taken as evidence in support of a xeric hypothetic ancestor of the genus.     

IAA-induced apogamy in Platycerium coronarium (Koenig) Desv. gametophytes cultured in vitro

Apogamous sporophytes were produced on Platycerium coronarium gametophytes cultured in the presence of indole-3-acetic acid (IAA). The percentage of apogamy as well as the total number of apogamous sporophytes produced per gametophyte clump were highest in the presence of 40 M IAA. When ethylene was allowed to accumulate in the culture vessel in the presence of an optimum level of IAA, the percentage and total number of apogamous sporophyte production decreased significantly. Using light microscope and confocal laser scanning microscope we have shown that nuclear size can be used as a quick parameter to estimate the ploidy level of P. coronarium.Abbreviations CLSM confocal laser scanning microscope - IAA Indole-3-acetic acid - MS Murashige and Skoog     

Developmental Morphology of Platycerium coronarium (Koenig) Desv

The growth habit and certain developmental aspects of Platyceriumcoronarium (stag's horn fern) are described. Starting from theprimordial stage, the nest and pendulous leaves develop to maturityin 90 and 80 days respectively. The fertile lobe, which is partof the pendulous leaf, reaches its maximum size in 40 days.The morphogenesis of the nest leaf is more variable, and itmatures and deteriorates earlier than the pendulous leaf. Theacrostichoid sorus formation is completed in 3 weeks from inceptionand spore dispersal takes place when the fertile lobe is about100 days old. The area of the fertile lobe and number of sporesproduced were determined. On Knop's agar medium the gametophytesdevelop in 2 months and 85 per cent of them are unisexual (bothmale and female) and 15 per cent bisexual. Less than 1 per centof the gametophytes give rise to sporophytes. The juvenile leavesare simple, displaying open dichotomous venation; the firstnest and pendulous leaves are produced 24 months after the dateof spore germination. Platycerium coronarium, stag's horn fern, leaf development, morphogenesis, spore production     

Herbarium survey on the genus Azolla (Salviniaceae) in Italy: distributive and taxonomic implications

An analysis of Italian herbarium specimens belonging to the genus Azolla was carried out using optical microscope and SEM techniques. According to recent international reviews, three micro-morphological characters have been considered for the specimen analysis: (i) number of leaf trichome cells, (ii) septation of glochidia and (iii) features of the macrospore perine. The analysis of the patterns concerning both vegetative and reproductive characters was complicated by the relatively low number of sporulated specimens stored in the Italian Herbaria. The analysis of the selected specimens showed the presence of two well distinct systematic groups. One group was characterized by bicellular leaf trichomes, pluriseptate glochidia and not-warty perine, traits that permitted to taxonomically attribute it to A. cristata. The second group was characterized by monocellular leaf trichomes, non-septate or uniseptate glochidia and warty perine, so that it could be taxonomically attributed to A. filiculoides Lam. This study confirmed the large use in the past of the name ‘A. caroliniana’ to indicate the species actually named A. cristata. In Italy, this species was found to be collected only in the past, while A. filiculoides was found to be present with both ancient and recent herbarium specimens.     

中国肿足蕨科一新记录属——翼囊蕨属（英文）

报道了中国肿足蕨科一新记录属——翼囊蕨属(Didymochlaena Desv.)。该属为泛热带分布的单型属,在中国为首次记录。本文结合原始文献对该属及其折囊蕨种的特征进行了详细描述。     

EinAecidium aufMyricaria germanica Desv.

Ohne Zusammenfassung     

Sporophyte and gametophyte development of Platycerium coronarium (Koenig) Desv. and P. grande (Fee) C. Presl. (Polypodiaceae) through in vitro propagation

The sporophyte and gametophyte development of Platycerium coronarium and P. grande were compared through ex situ propagation using in vitro culture technique and under greenhouse and field conditions.The morphology of the sporophyte and gametophyte, type of spore germination and prothallial development of P. coronarium and P. grande were documented. Gametophytes of P. coronarium and P. grande were cultured in vitro using different media. The gametophytes were then transferred and potted in sterile chopped Cyathea spp. (anonotong) roots and garden soil for sporophyte formation. Sporophytes (plantlets) of the two Platycerium species were attached on the slabs of anonotong and on branches and trunks of Swietenia macrophylla (mahogany) under greenhouse and field conditions.Sporophyte morphology of P. coronarium and P. grande varies but not their gametophyte morphology. P. coronarium and P. grande exhibited rapid spore germination and gametophyte development in both spore culture medium and Knudson C culture medium containing 2% glucose. Gametophytes of P. coronarium and P. grande transferred to potting medium produced more number of sporophytes while the gametophytes inside the culture media did not produce sporophytes. Sporophytes of P. grande attached on mahogany branches produced more number of leaves with bigger leaf area than those attached on anonotong slabs. Likewise, sporophytes of P. coronarium attached on mahogany branches and anonotong slabs did not develop new leaves during two weeks monitoring and are still in a period of adjustment to its environment. Sporophytes of P. grande grown or attached on the trunk of mahogany trees in the field and under shaded environment favored their growth.     

Die Fossilen Vertreter der Gattung Salvinia Mich. I. Ein Beitrag zur Kenntnis der Mikrosporangien der Salvinia Formosa Heer

Zusammenfassung Schon in meiner 1928 erschienenen monographischen Studie über die Verbreitung und die Arten der HydropteridengattungSalvinia Mich. im Tertiär des Vogelsberges und der Wetterau (1), konnte ich S. 146–147 auf die bemerkenswerte Entdeckung vonSalviniasporangien in der miozänen Braunkohle des Himmelsberges bei Fulda hinweisen. Bereits damals teilte ich auch einige Einzelheiten über das Vorkommen mit, wollte mir aber äußerer Umstände halber und wegen der vorgefaßten Absicht, zunächst nur die Schwimmblätter zum Gegenstand einer eingehenden Untersuchung zu machen, die nähere Beschreibung noch vorbehalten.Hier sei dies nun nachgeholt; der Darstellung ist gleichzeitig eine Anzahl Mikrophotogramme der Mikrosporangien und ihres Sporeninhaltes beigegeben, da meiner Meinung, die auch von einem Referenten (Kräusel) der genannten Arbeit geteilt wird, gute Abbildungen des Fundes zu Vergleichs- und anderen Zwecken als sehr wünschenswert erscheinen.Mit 8 Textabbildungen.     

Ricerche Sulla Vegetazione Della Valsesia. VII. Notholaena Marantae (L.) Desv. in Valsesia

Abstract

Researches on the vegetation of Valsesia. VII. «Notholaena marantae» (L.) Desv. in the Sesia Valley. — It's hereby described a station of Notholaena marantae (L.) Desv. near «Giavina Rossa» (Valsesia-Piedmont, Italy). This is probably the only place in the whole valley. The fern grows in a kind of ground formed by peridotitic rocks and on a little dry wall supporting the debris. The fern presence in a wooden valley, and under an oceanic climate could be a very interesting subject for hystorical geobotanic researches.     

Cytotoxic compounds from invasive giant salvinia (Salvinia molesta) against human tumor cells

Giant salvinia (Salvinia molesta) is one of the most noxious invasive species in the world. Our bioactivity-guided fractionation of ethanol extract of giant salvinia led to the isolation of 50 compounds. Of the six new compounds (1–6), salviniol (1) is a rare abietane diterpene with a new ferruginol-menthol coupled skeleton and both salviniside I (2) and salviniside II (3) are novel benzofuran glucose conjugates with unique 10-membered macrodiolide structures. Sixteen abietane diterpenes (1, 7–17, and 19–22) demonstrated in vitro activities against human tumor cells, and 7 and 8 showed selective cytotoxicity to tumor cells over normal cells.     

香雪球的组织培养和快速繁殖

1植物名称 香雪球[Lobularia maritima（L．）Desv．],别名小白花、庭荠。 2材料类别无菌苗的顶芽和带腋芽茎段。     

Retrotransposon‐based genetic diversity of Deschampsia antarctica Desv. from King George Island (Maritime Antarctic)

Deschampsia antarctica Desv. can be found in diverse Antarctic habitats which may vary considerably in terms of environmental conditions and soil properties. As a result, the species is characterized by wide ecotypic variation in terms of both morphological and anatomical traits. The species is a unique example of an organism that can successfully colonize inhospitable regions due to its phenomenal ability to adapt to both the local mosaic of microhabitats and to general climatic fluctuations. For this reason, D. antarctica has been widely investigated in studies analyzing morphophysiological and biochemical responses to various abiotic stresses (frost, drought, salinity, increased UV radiation). However, there is little evidence to indicate whether the observed polymorphism is accompanied by the corresponding genetic variation. In the present study, retrotransposon‐based iPBS markers were used to trace the genetic variation of D. antarctica collected in nine sites of the Arctowski oasis on King George Island (Western Antarctic). The genotyping of 165 individuals from nine populations with seven iPBS primers revealed 125 amplification products, 15 of which (12%) were polymorphic, with an average of 5.6% polymorphic fragments per population. Only one of the polymorphic fragments, observed in population 6, was represented as a private band. The analyzed specimens were characterized by low genetic diversity (uH_e = 0.021, I = 0.030) and high population differentiation (F_ST = 0.4874). An analysis of Fu's F_S statistics and mismatch distribution in most populations (excluding population 2, 6 and 9) revealed demographic/spatial expansion, whereas significant traces of reduction in effective population size were found in three populations (1, 3 and 5). The iPBS markers revealed genetic polymorphism of D. antarctica, which could be attributed to the mobilization of random transposable elements, unique features of reproductive biology, and/or geographic location of the examined populations.