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1.
Light intensity preferences of Procambarus clarkii (Girard, 1852) were evaluated in an indoor fiber glass trough, consisting of five equal length (4 m) zones with light intensities
varying from dark to 1,000 lux (L), situated at the ‘Las Vegas del Guadiana’ fish farm in Extremadura (Spain). Four trials
were performed, with different light zones set in three at sections of the trough, and the fourth providing a control with
one light density (300 L) throughout the trough. Whereas the highest intensity (1,000 L) was preferred in trials 1 and 2,
the dimmest condition (D) was favored in trial 3. Crayfish showed a strong preference to both outside ends of the trough,
and the “end effect” of the trough on position of the crayfish was found to be stronger than the impact of light intensity. 相似文献
2.
3.
This study analyzes the population structure and dynamics of an invasive population of Procambarus clarkii (Girard, 1852) in a Mediterranean wetland using the Bhattacharya’s and Von Bertallanfy’s analytical methods. The main purpose
was to collect biological data necessary for the management of this nuisance species. A maximum of five age classes were identified
for both sexes, three of which being composed of a few or zero individuals. Age classes were classified into two subgroups––spring
(SpL) and summer (SuL) lines––on the basis of the different hatching periods. Individuals of SpL showed a faster growth rate
and reached a larger body size than those belonging to SuL, probably because they were able to grow for a longer time. No
between-sex differences were found in growth patterns except for the asymptotic length (L∞), which was reached faster by the females. Other population properties were analyzed, such as a high mortality rate, a maximum
longevity of 4 years, and a low mean life-time (<12 months). Finally, a relatively small fraction of individuals seemed to
survive after the first reproductive peak in spring. Consequently, the structure and dynamics of the study population seem
to reveal its stability and spreading potential, as a confirmation of the invasiveness of P. clarkii in Mediterranean wetlands.
Handling editor: P. Viaroli 相似文献
4.
Aphanomyces astaci (Saprolegniales, Oomycetes) is classified among the 100 world’s worst invasive species. This species is endemic to North
America and has been introduced into Europe by imports of their hosts, the North American crayfish species. As a consequence,
extensive mass mortalities involved several populations of the European crayfish. Here, we checked its occurrence in four
Italian populations of Procambarus clarkii, the most widespread alien crayfish in Italy. Digital image analyses and image processing techniques were used to select
micro-melanized areas in the subabdominal cuticle of 2–10 crayfish per population. All the selected areas tested positive
for A. astaci ITS nrDNA specific primers; moreover, the obtained sequences clearly corresponded to A. astaci, thus revealing that P. clarkii is an active carrier of this oomycete in Italy. Decisions are to be urgently made to control the spread of both P. clarkii and A. astaci for the conservation of the indigenous crayfish biodiversity. 相似文献
5.
Temporary ponds, acknowledged for their conservation value, are colonized by the invasive crayfish Procambarus clarkii. We have tested the consequences of this colonization for the ecosystem under two contrasted scenarios: one single individual arrival or three individuals arrival. We recreated the temporary pond ecosystem in 1 m2 tanks to investigate the impact of the two crayfish densities. We studied the macrophyte community composition and abundance, chlorophyll a and total suspended solids concentrations, and the diversity and functional composition of micro-crustacean and macro-invertebrate communities. We observed a reduction of macrophyte biomass in experimental crayfish mesocosms in comparison with control tanks, nearly 80 and 40% less in 3 and 1 crayfish/m2 tanks, respectively. The macrophyte community shifted, followed by a filamentous algae development, an increase of bare sediment and turbidity in crayfish tanks. The macro-invertebrate community suffered a richness loss of 28 and 22%, in 3 and 1 crayfish/m2 tanks, respectively. Functionally, macro-invertebrate diversity reduction most strongly affected the grazer, detritivore and predator trophic groups. Microcrustaceans seemed not to be affected by the introduction of the crayfish. The introduction of the crayfish greatly altered the ecosystem structure and subsequently the ecosystem functioning. 相似文献
6.
Crayfish establish social dominance hierarchies through agonistic interactions, and these hierarchies are maintained through
assessment of social status. Chemical signals influence several aspects of fighting behavior, but the specific chemosensory
sensilla involved in detecting these signals in crayfish are unknown. The goal of our study was to examine the importance
of aesthetasc sensilla—olfactory sensors on the antennules of decapod crustaceans—in regulating changes in fighting behavior
in crayfish, Procambarus clarkii, over the course of repeated pairings. We selectively ablated aesthetascs from pairs of crayfish after the first day of trials
and compared the behavior of these ablated animals to that of pairs of intact controls. Results show that unablated crayfish
significantly decreased the number and duration of fights over repeated pairings, whereas crayfish lacking aesthetascs continued
to engage in similar amounts of fighting across all three trial days. This difference shows that aesthetascs regulate fighting
behavior in P. clarkii. 相似文献
7.
This study examines the expression of the photopigment gene in the developing retina of the freshwater crayfish Procambarus clarkii(Crustacea, Malacostraca, Decapoda). Both sense and anti-sense RNA probes were used for in situ hybridization (ISH) of whole embryos collected at various stages during development. A characteristic of retinal development is the formation of screening pigment in the retinular cells of the retinal ommatidia. This pigmentation is seen as a band that begins at the lateral side of the retinal field and progresses medially. At hatching the retina is approximately 50% pigmented. ISH of whole embryos shows that expression of the photopigment gene by the retinular cells correlates with the extent of the screening pigment band in the retina and with the presence of rhabdoms within the ommatidia. Sections taken through embryos after being hybridized indicate that staining is localized in the cytoplasm of the retinular cells and in the axonal region below the basement membrane. No staining reaction was seen in the rhabdoms of older ommatidia. ISH staining was also seen at the anterior midline of the protocerebrum where extraretinal photoreceptors have been reported. The data presented here show a close correlation of opsin expression within the retinular cells of the ommatidia and the formation of the very early rhabdoms, similar to Drosophila. The results will be discussed in relation to recent studies in Drosophila that suggest rhodopsin plays a role in effecting the organization of the terminal web-like cytoskeleton at the base of the developing rhabdom microvilli. 相似文献
8.
Impacts of invasive species may manifest most strongly if these organisms are highly distinct functionally from the native species they often replace. Yet, should we expect functional differences between native and invasive species of generalist organisms like freshwater crayfish? Some existing evidence has pointed to native and invasive crayfish species as ecologically equivalent. We contribute to this literature by comparing the trophic niches of the globally invasive crayfishes Pacifastacus leniusculus and Procambarus clarkii, by applying carbon and nitrogen stable isotope analyses to replicated allopatric (alone) and sympatric (together) lake populations in western Washington State, USA, where P. clarkii has been recently introduced and P. leniusculus is presumed native. Our study corrected for potential inherent differences in lake food webs as a consequence of lake abiotic or biotic characteristics using random effects in linear mixed effects models. We found that although overall trophic niche size or area of these species was not significantly different, P. leniusculus was significantly higher in trophic position than P. clarkii when also accounting for the effects of body size, sex, and lakes as random effects. This pattern of increased trophic position of P. leniusculus over P. clarkii was conserved over time in one sympatric lake for which we had data over multiple years. Cumulatively, our findings point to trophic differences between the globally cosmopolitan crayfishes P. leniusculus and P. clarkii, particularly when accounting for the ways that ecosystem context can affect food web structure of communities and the trophic resources available to these consumers. 相似文献
9.
Laura Aquiloni Sara Brusconi Elena Cecchinelli Elena Tricarico Giuseppe Mazza Annalisa Paglianti Francesca Gherardi 《Biological invasions》2010,12(11):3817-3824
The red swamp crayfish, Procambarus clarkii, is a paradigmatic invader of freshwater systems. Several attempts have been made to mitigate its multiple impacts but none
was successful. Among the different methods proposed, the use of the European eel (Anguilla anguilla) as an indigenous predator is promising but the available information about its predatory ability on crayfish is to date
scanty. To fill this gap in knowledge, we ran three experiments in wetlands and irrigation ditches in Italy. The first experiment,
in the laboratory, was aimed at quantifying the extent of predation by eels on crayfish, the second, in enclosures, the size
classes of crayfish mainly preyed and the possible effect of the eels on P. clarkii behaviour, and the third, in the field, its ability to effectively reduce crayfish populations. Results showed that eels
prey on small-sized or soft crayfish, attacking them from the back; an indirect effect was to reduce crayfish trophic activity,
which in turn might increases crayfish mortality due to starvation and decreases impact on the community. However, as shown
in the field, the use of eels should be appropriately calibrated to the context of application. Taken together, our results
show that eels might be used as a complement to the traditional trapping method. However, additional studies are necessary
to understand the adequate number of eels to be introduced and to develop appropriate methods for quantifying such effects. 相似文献
10.
11.
The introduction of some crustacean species has produced alterations of freshwater environments and declines of native species worldwide. The red swamp crayfish, Procambarus clarkii Girard, was introduced in the Southwest Iberian Peninsula in the 1970’s, producing severe impacts on rice agriculture and on native biota such as macrophytes, gastropods, native crayfish and amphibians. We studied the distribution of P. clarkii in two areas of SW Iberian Peninsula: the Sado River basin (SW Portugal), an area colonized by this species around 1990, and the Parque Natural del Entorno de Doñana (SW Spain), colonized soon after its introduction in the Iberian Peninsula, in the 1970’s. Our main goal was to determine which factors limit crayfish distribution, which could help to identify the most effective management practices to contain its spread. Procambarus clarkii was found in most types of water bodies, including small and shallow ones. Distance to a crayfish source was the single predictor variable explaining crayfish occurrence in most types of habitats and in both areas. The only exception was for the Sado permanent stream points, where crayfish presence was negatively affected by an interaction between elevation and flow velocity. Other habitat characteristics have apparently little or no importance for its successful colonization. Moreover, this study indicated that overland dispersal is apparently a frequent phenomenon in this species. Our findings can be used to determine which habitats are most likely to be colonized by the crayfish and to develop practical measures which may limit its spread and minimize its impacts. 相似文献
12.
Two repeated DNA sequences isolated from a partial genomic DNA library of Helianthus annuus, p HaS13 and p HaS211, were shown to represent portions of the int gene of a Ty3 /gypsy retroelement and of the RNase-Hgene of a Ty1 /copia retroelement, respectively. Southern blotting patterns obtained by hybridizing the two probes to BglII- or DraI-digested genomic DNA from different Helianthus species showed p HaS13 and p HaS211 were parts of dispersed repeats at least 8 and 7 kb in length, respectively, that were conserved in all species studied. Comparable hybridization patterns were obtained in all species with p HaS13. By contrast, the patterns obtained by hybridizing p HaS211 clearly differentiated annual species from perennials. The frequencies of p HaS13- and p HaS211-related sequences in different species were 4.3x10(4)-1.3x10(5) copies and 9.9x10(2)-8.1x10(3) copies per picogram of DNA, respectively. The frequency of p HaS13-related sequences varied widely within annual species, while no significant difference was observed among perennial species. Conversely, the frequency variation of p HaS211-related sequences was as large within annual species as within perennials. Sequences of both families were found to be dispersed along the length of all chromosomes in all species studied. However, Ty3 /gypsy-like sequences were localized preferentially at the centromeric regions, whereas Ty1/ copia-like sequences were less represented or absent around the centromeres and plentiful at the chromosome ends. These findings suggest that the two sequence families played a role in Helianthusgenome evolution and species divergence, evolved independently in the same genomic backgrounds and in annual or perennial species, and acquired different possible functions in the host genomes. 相似文献
13.
Noel H. Holmgren 《Brittonia》2018,70(1):115-139
A revision of Penstemon sect. Saccanthera subsect. Serrulati includes a new species (P. salmonensis), a new variety (P. triphyllus var. infernalis), and the elevation of a subspecies to species (P. curtiflorus), bringing the total number of species to eight, which are keyed and described, complete with nomenclature and type citations. 相似文献
14.
A genetic transformation system has been developed for callus cells of Crataegus
aronia using Agrobacterium
tumefaciens. Callus culture was established from internodal stem segments incubated on Murashige and Skoog (MS) medium supplemented with
5 mg l−1 Indole-3-butyric acid (IBA) and 0.5 mg l−1 6-benzyladenine (BA). In order to optimize the callus culture system with respect to callus growth and coloration, different
types and concentrations of plant growth regulators were tested. Results indicated that the best average fresh weight of red
colored callus was obtained on MS medium supplemented with 2 mg l−1 2,4-dichlorophenoxyacetic acid (2,4-D) and 1.5 mg l−1 kinetin (Kin) (callus maintenance medium). Callus cells were co-cultivated with Agrobacterium harboring the binary plasmid pCAMBIA1302 carrying the mgfp5 and hygromycin phosphotransferase (hptII) genes conferring green fluorescent protein (GFP) activity and hygromycin resistance, respectively. Putative transgenic calli
were obtained 4 weeks after incubation of the co-cultivated explants onto maintenance medium supplemented with 50 mg l−1 hygromycin. Molecular analysis confirmed the integration of the transgenes in transformed callus. To our knowledge, this
is the first time to report an Agrobacterium-mediated transformation system in Crataegus
aronia. 相似文献
15.
Studying Pneumocystis has proven to be a challenge from the perspective of propagating a significant amount of the pathogen in a facile manner.
The study of several fungal pathogens has been aided by the use of invertebrate model hosts. Our efforts to infect the invertebrate
larvae Galleria
mellonella with Pneumocystis proved futile since P. murina neither caused disease nor was able to proliferate within G. mellonella. It did, however, show that the pathogen could be rapidly cleared from the host. 相似文献
16.
J. A. Aguilar-Alberola F. Mesquita-Joanes S. López A. Mestre J. C. Casanova J. Rueda A. Ribas 《Hydrobiologia》2012,688(1):63-73
The American red swamp crayfish Procambarus clarkii (Girard, 1852) was introduced in 1973 into the Iberian Peninsula for commercial purposes. As a result of both the expansion
from the Iberian Peninsula and, probably, further introductions in other European countries, now it is widely distributed
throughout much of Europe. The ecological impacts of this invading crayfish have received increasing attention, but nothing
is known about its symbiotic entocytherid ostracods outside the American continent. The present survey has examined more than
200 crayfishes from 12 localities distributed over a wide area of Eastern Spain. Entocytherid ostracods were extracted from
individual crayfishes and they were identified, counted, assigned to developmental instars and sexed. In all the study locations
but one, we found at least one crayfish individual infected by entocytherid ostracods and the species determined was the same
in all cases: Ankylocythere sinuosa (Rioja, 1942). The number of ostracods on individual P. clarkii varied notably in relation to crayfish size and also differed significantly among sampling sites. The crayfish size effects
on ostracod densities might be related to the amount of resources and to the crayfish age and moulting frequency affecting
ostracod distribution and population structure. In addition, the spatial variation in ostracod densities could also be related
to site-specific habitat traits and the variability of crayfish population dynamics. Our study represents the first citation
of an alien entocytherid species in Europe and demonstrates its wide distribution in the Iberian Peninsula. Further research
is needed to know the potential effects of this ostracod species on the ecology of P. clarkii and of native species, with implications on the management of this aquatic invader. 相似文献
17.
Luis Hernández Alejandro M. Maeda-Martínez Gorgonio Ruiz-Campos Gabino Rodríguez-Almaraz Fernando Alonzo-Rojo Juan Carlos Sainz 《Biological invasions》2008,10(7):977-984
The red crayfish Procambarus clarkii, which is native to southcentral USA and northeastern Mexico, has been successfully introduced into several countries around
the world. This study documents the geographic expansion of the exotic red crayfish in Mexico and discusses the consequence
of a greater propagation of this species in Mexican inland waters. New state records of this crayfish in the Baja California
peninsula and in the states of Durango and Sinaloa indicate its progressive dispersion. The propagation of P. clarkii in Mexico has been caused mainly by human introduction, but it is also facilitated because of the species’ tolerance to an
ample range of environmental conditions. Because of the invasive capability of P. clarkii, we suspect that this exotic species is competing for habitat and food with native freshwater shrimp of the genus Macrobrachium in many sites of northern Mexico. 相似文献
18.
The maT clade of transposons is a group of transposable elements intermediate in sequence and predicted protein structure to mariner and Tc transposons, with a distribution thus far limited to a few invertebrate species. We present evidence, based on searches of publicly available databases, that the nematode Caenorhabditis briggsae has several maT-like transposons, which we have designated as CbmaT elements, dispersed throughout its genome. We also describe two additional transposon sequences that probably share their evolutionary history with the CbmaT transposons. One resembles a fold back variant of a CbmaT element, with long (380-bp) inverted terminal repeats (ITRs) that show a high degree (71%) of identity to CbmaT1. The other, which shares only the 26-bp ITR sequences with one of the CbmaT variants, is present in eight nearly identical copies, but does not have a transposase gene and may therefore be cross mobilised by a CbmaT transposase. Using PCR-based mobility assays, we show that CbmaT1 transposons are capable of excising from the C. briggsae genome. CbmaT1 excised approximately 500 times less frequently than Tcb1 in the reference strain AF16, but both CbmaT1 and Tcb1 excised at extremely high frequencies in the HK105 strain. The HK105 strain also exhibited a high frequency of spontaneous induction of unc-22 mutants, suggesting that it may be a mutator strain of C. briggsae. 相似文献
19.
Notocactus scopa cv. Soonjung was subjected to in planta Agrobacterium tumefaciens-mediated transformation with vacuum infiltration, pin-pricking, and a combination of the two methods. The pin-pricking combined with vacuum infiltration (20-30 cmHg for 15 min) resulted in a transformation efficiency of 67-100%, and the expression of the uidA and nptII genes was detected in transformed cactus. The established in planta transformation technique generated a transgenic cactus with higher transformation efficiency, shortened selection process, and stable gene expression via asexual reproduction. All of the results showed that the in planta transformation method utilized in the current study provided an efficient and time-saving procedure for the delivery of genes into the cactus genome, and that this technique can be applied to other asexually reproducing succulent plant species. 相似文献