共查询到20条相似文献,搜索用时 31 毫秒
1.
Plant regeneration from mesophyll protoplasts of pepper,Capsicum annuum
L. cv. California Wonder has been demonstrated via shoot organogenesis. Protoplasts isolated from fully expanded leaves of
3-week-old axenic shoots when cultured in TM medium supplemented with 1 mg l
−1
NAA, 1 mg l
−1
2,4-D, 0 5 mg l
−1
BAP (CM 1) resulted in divisions with a frequency ranging from 20–25 %. Antioxidant ascorbic acid and polyvinylpyrrolidone
(PVP) in the medium and incubation in the dark helped overcome browning of protoplasts. Microcalli and macrocalli were formed
in TM medium containing 2 mg l
−1
NAA and 0.5 mg l
−1
BAP (CM II) and MS gelled medium containing 2 mg 1
−1
NAA and 0 5 mg 1
−1
BAP (CM III), respectively. Regeneration of plantlets was possible via caulogenesis. Microshoots, 2–5 percallus appeared on
MS gelled medium enriched with 0.5 mg l
−1
IAA, 2mg l
−1
GA and l0mg l
−1
BAP (CM IVc). Rooting of microshoots was obtained on half strength gelled medium containing 1 mg l
−1
NAA and 0.5mg l
−1
BAP. Protoplasts isolated from cotyledons failed to divide and degenerated eventually. 相似文献
2.
A flat plate, multi-pass air lift reactor (FPALR) for the culture of photosynthetic organisms was constructed from twin wall
acrylic sheet and its performance characterised. When operated at an air input of 2.01 min−1 the multi-pass system had a Reynolds number of 5200 indicating fully turbulent flow. Chlorella vulgaris 211/11c was found to have a stationary phase biomass of 1.48 g 1−1 when grown in the flat plate air lift reactor (FPALR) at 100 μmol m−2s−1 compared to 1.11 g 1−1 when cultured in the continually stirred tank reactor (CSTR) at the same PFD (photon flux density). The same organism cultured
at 200 μmol m−2s−1 achieved a stationary phase biomass of 1.71 g 1−1 in the FPALR. In contrast, Scenedesmus sp. produced a stationary phase biomass of 2.27 g1−1 and 1.27 g1−1, when cultured at 100 μmol m−2s−1 in the FPALR and the CSTR respectively. The growth rates of both organisms were also higher in the PFALR. 相似文献
3.
The factors controlling biomass production and the synthesis of astaxanthin esters in the microalga Haematococcus pluvialis (CCAP 34/7) have been investigated using a statistical approach employing response surface methodology (RSM). The culture
conditions required for optimal growth and carotenogenesis in this alga are very different. Of particular importance is the
photon flux density: for growth the optimum is 50–60 μmol m−2 s−1 whereas the optimum for astaxanthin synthesis is much higher at ∼-1600 μmol m−2 s−1. The addition of low levels of NaCl to the medium also stimulates to a small extent synthesis of astaxanthin, but photon
flux density remains the overriding factor. The optimal temperature for this strain is quite low at 14–15 °C. RSM has been
shown to be a rapid and effective technique leading to the optimisation of algal culture conditions. This statistical approach
can be applied readily to the majority of microalgae and their products. 相似文献
4.
M. R. de la Peña 《Journal of applied phycology》2007,19(6):647-655
Two series of experiments were conducted to determine suitable growth factors for the mass propagation of the local algal
isolate Amphora sp. A higher growth rate of 0.2 doubling (μ) day−1 was attained at a lower photosynthetic photon flux density (PPFD; 11.4 μmol photon m−2s−1) compared to cultures exposed to higher levels of PPFD (16.1 μmol photon m−2s−1, −0.1 μ day −1; 31.3 μmol photon m−2s−1, 0.0 μ day−1). Cultures located inside the laboratory had a significantly higher cell density (133 × 104 cells cm−2) and growth rate (0.3 μ day−1) compared to those located outdoors (100 × 104 cells cm−2, 0.2 μ day−1). A comparison of nutrient medium across two locations showed that lipid content was significantly higher in cultures enriched
with F/2MTM (macronutrients + trace metals) and F/2MV (macronutrients + vitamins). Saturated fatty acids were also present
in high concentrations in cultures enriched with F/2M (macronutrients only). Significantly higher amounts of saturated fatty
acids were observed in cultures located outdoors (33.1%) compared to those located indoors (26.6%). The protein, carbohydrates
and n-6 fatty acid content of Amphora sp. were influenced by the location and enrichment of the cultures. This study has identified growth conditions for mass
culture of Amphora sp. and determined biochemical composition under those culture conditions.
Presented at the 6th Meeting of the Asian Pacific Society of Applied Phycology, Manila, Philippines. 相似文献
5.
Pythium porphyrae is a fungal pathogen responsible for red rot disease of the seaweed Porphyra (Rhodophyta). Infection forecasts of Porphyra by P. porphyrae were estimated from the epidemiological observations of Porphyra thalli and numbers of zoospore of P. porphyrae in laboratory and cultivation areas. Four features of forecasting infections were determined by relating zoospore concentrations to the incidence of thallus infection; infection (in more than 1000 zoospores L−1), microscopic infection [less than 2 mm in diameter of lesion (in from 2000 to 3000 zoospores L−1)], macroscopic infection [more than 2 mm in diameter of lesion (in from 3000 to 4000 zoospores L−1), and thallus disintegration (in more than 4000 zoospores L−1). High zoospore concentrations led to more infection. The tendency that zoospore concentration of P. porphyrae increased with the rate of infection of Porphyra thalli was generally observed in forecasting infections in both the laboratory and in cultivation areas. Based on the Porphyra cultivation areas, the accuracy and consistency of forecasting infections suggest that this method could be employed to manage and control red rot disease. 相似文献
6.
J. G. Wakibia J. J. Bolton D. W. Keats L. M. Raitt 《Journal of applied phycology》2006,18(3-5):565-573
As a possible means of improving the livelihoods of local villagers, off-bottom rope cultivation of commercial eucheumoids was investigated on the southern Kenyan coast at three sites, representative of the variety of environments. The morphotypes used were brown Eucheuma denticulatum and green and brown Kappaphycus alvarezii. The study was carried out over a 15 month period from August 2001 until October 2002. Relative growth rates were highest at a sandy flat in a mangrove system (Gazi; 5.6% d−1), and lowest in an intertidal reef flat (Kibuyuni; 3.2% d−1) with a lagoon being intermediate (Mkwiro; 4.8% d−1). The brown E. denticulatum had the highest growth rate of 4.7% d−1 compared to the green and brown K. alvarezii which were 4.3% d−1 and 4.2% d−1, respectively. Growth was more variable at Kibuyuni and Mkwiro. The growth was higher during the southeast monsoon (4.7% d−1) than during the northeast monsoon (4.0% d−1). This is part of a larger study and the effects of water motion, salinity, temperature, thallus nitrogen, and ‘ice-ice’ syndrome on growth of morphotypes is discussed. The water motion was observed to increase thallus nitrogen and hence the growth of eucheumoids. The ‘ice-ice’ condition affected both brown E. denticulatum and brown K. alvarezii but not green K. alvarezii. The results suggest that commercial cultivation of eucheumoids in Kenya will be feasible. 相似文献
7.
Andrea Kodym Stefan Hollenthoner Francisco Javier Zapata-Arias 《In vitro cellular & developmental biology. Plant》2001,37(2):237-242
Summary Daylight instead of artificial light was exploited for the in vitro culture of banana. Tubular skylights rediverted natural light into an interior enelosed room whereby each skylight, available
for ca. US$600, could sufficiently illuminate an area of 3–5 m2. The maintenance-free system allowed only a minimum of heat transfer and no cooling was necessary. The culture room required
no electricity supply and under our conditions savings on costs for electricity of US$6 m−2 wk−1 were achieved, as compared to a standard growth room equipped with artificial lighting and controlled photoperiod and temperature
regimes. Under natural light conditions, micropropagated plantlets were well developed at mean photosynthetic photon flux
densities (PPFD) of 5–13 μmol m−2 s−1 and photoperiods of 9–14 h. Micropropagation rates were either the same or significantly higher than under artificial lighting.
Single shoots on rooting medium showed some symptoms of etiolation, yet acclimatization rates averaged 95%. A step-like culture
rack. rather than a vertical one, permitted uniform plant growth on all levels. This paper describes an established micropropagation
system of low cost and simplicity. 相似文献
8.
Y. Kitaya L. Xiao A. Masuda T. Ozawa M. Tsuda K. Omasa 《Journal of applied phycology》2008,20(5):737-742
Symbiotic dinoflagellates of the species Amphidinium are expected to be pharmaceutically useful microalgae because they produce antitumor macrolides. A microalgae production
system with a large number of cells at a high density has been developed for the efficient production of macrolide compounds.
In the present study, the effects of culture conditions on the cellular growth rate of dinoflagellates were investigated to
determine the optimum culture conditions for obtaining high yields of microalgae. Amphidinium species was cultured under conditions with six temperature levels (21–35°C), six levels of photosynthetic photon flux density
(15–70 μmol photons m−2 s−1), three levels of CO2 concentration (0.02–0.1%), and three levels of O2 concentration (0.2–21%). The number of cells cultured in a certain volume of solution was monitored microscopically and the
cellular growth rate was expressed as the specific growth rate. The maximum specific growth rate was 0.022 h−1 at a temperature of 26°C and O2 concentration of 5%, and the specific growth rate was saturated at a CO2 concentration of 0.05%, a photosynthetic photon flux density of 35 μmol photons m−2 s−1 and a photoperiod of 12 h day−1 upon increasing each environmental parameter. The results demonstrate that Amphidinium species can multiply efficiently under conditions of relatively low light intensity and low O2 concentration. 相似文献
9.
The response of barley (Hordeum vulgare L. cv. Akcent) to various photosynthetic photon flux densities (PPFDs) and elevated [CO2] [700 μmol (CO2) mol−1; EC] was studied by gas exchange, chlorophyll (Chl) a fluorescence, and pigment analysis. In comparison with barley grown under ambient [CO2] [350 μmol (CO2) mol−1; AC] the EC acclimation resulted in a decrease in photosynthetic capacity, reduced stomatal conductance, and decreased total
Chl content. The extent of acclimation depression of photosynthesis, the most pronounced for the plants grown at 730 μmol
m−2 s−1 (PPFD730), may be related to the degree of sink-limitation. The increased non-radiative dissipation of absorbed photon energy for
all EC plants corresponded to the higher de-epoxidation state of xanthophylls only for PPFD730 barley. Further, a pronounced decrease in photosystem 2 (PS2) photochemical efficiency (given as FV/FM) for EC plants grown at 730 and 1 200 μmol m−2 s−1 in comparison with AC barley was related to the reduced epoxidation of antheraxanthin and zeaxanthin back to violaxanthin
in darkness. Thus the EC conditions sensitise the photosynthetic apparatus of high-irradiance acclimated barley plants (particularly
PPFD730) to the photoinactivation of PS2.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
10.
The kinetic parameters Km and Vmax for urea uptake by Melosira italica were determined at 160 μeinsteins m−2 s−1 and in the dark. The transport systems showed an affinity for the substrate and a storing capacity in the dark (Km = 65.07 μM; Vmax = 2.18 nmoles 105 cells −1 h−1) greater than under 160 μE m−2 s −1 (Km = 111.2 μM; Vmax = 1.11 nmoles 105 cells−1 h−1). Similarly, a reduction in consumption rate of urea under increasing photon flux densities was observed. The use of an inhibitor
(potassium cyanide) indicated that the uptake process requires metabolic energy. That urea transport is more important in
darkness, may constitute a survival strategy in which this compound is utilized by cells mainly during heterotrophic growth. 相似文献
11.
Ziguo Zhao Fengjuan Zhao Jianting Yao Jingmei Lu Put. O. AngJr. Delin Duan 《Journal of applied phycology》2008,20(5):925-931
Morphology and culture studies on germlings of Sargassum thunbergii (Mertens et Roth) Kuntze were carried out under controlled laboratory conditions. Growth characteristics of these germlings
grown under different temperatures (from 10 to 25°C), irradiances (from 9 to 88 μmol photons m−2 s−1), and under blue and white light conditions are described. The development of embryonic germlings follows the classic “8
nuclei 1 egg” type described for Sargassaceae. Fertilized eggs spent 5–6 h developing into multicellular germlings with abundant
rhizoids after fertilization. Under conditions of 20°C, 44 μmol photons m−2 s−1 and photoperiod of 12 h, young germlings with one or two leaflets reached 2–3 mm in length after 8 weeks. Temperature variations
(10, 15, 20, 25°C) under 88 μmol photons m−2 s−1 significantly influenced the growth rate within the first week, although this effect became less obvious after 8 weeks, especially
at 15 and 20°C. Variation in germling growth was highly significant under different irradiances (9, 18, 44, 88 μmol photons
m−2 s−1) at 25°C. Low temperature (10°C) reduced germling growth. Growth of germlings cultured under blue light was lower than in
white light. Optimal growth of these germlings occurred at 25°C and 44 μmol photons m−2 s−1. 相似文献
12.
Domínguez-Bocanegra AR Ponce-Noyola T Torres-Muñoz JA 《Applied microbiology and biotechnology》2007,75(4):783-791
Phaffia rhodozyma (now Xanthophyllomyces dendrorhous) and Haematococcus pluvialis are known as the major prominent microorganisms able to synthesize astaxanthin natural pigment. Important research efforts
have been made to determine optimal conditions for astaxanthin synthesis. When the focus is on astaxanthin production, the
maximal reported value of 9.2 mg/g cell is obtained within H. pluvialis grown on BAR medium, under continuous illumination (345 μmol photon m−2 s−1) and without aeration. Whereas fermentation by mutated R1 yeast grown on coconut milk produced 1,850 μg/g yeast. However,
when looking at astaxanthin productivity, the picture is slightly different. The figures obtained with P. rhodozyma are rather similar to those of H. pluvialis. Maximal reported values are 170 μg/g yeast per day with a wild yeast strain and 370 μg/g yeast per day with mutated R1 yeast.
In the case of H. pluvialis, maximal values ranged from 290 to 428 μg/g cell per day depending on the media (BG-11 or BAR), light intensity (177 μmol
photon m−2 s−1), aeration, etc. The main aim of this work was to examine how astaxanthin synthesis, by P. rhodozyma and H. pluvialis, could be compared. The study is based on previous works by the authors where pigment productions have been reported. 相似文献
13.
Andrzej Kaźmierczak 《Acta Physiologiae Plantarum》1999,21(4):345-348
Method for simultaneous measurement of gibberellic acid was applied using capillary zone electrophoresis. Gibberellic acid
was identified in extracts of apical part of thallus of Chara vulgaris L. The amount of gibberellins measured on the basis of activity determined by the micro-drop bioassay (59.8 mg·kg−1; with gibberellic acid as a standard) was comparable with that estimated by capillary electrophoresis (54.9 mg·kg−1). 相似文献
14.
Anondho Wijanarko Dianursanti Antonius Yudi Sendjaya Heri Hermansyah Arief Budi Witarto Misri Gozan Bondan Tiara Sofyan Kazuhiro Asami Kazuhisa Ohtaguchi Roekmijati Widaningroem Soemantojo Seung Koo Song 《Biotechnology and Bioprocess Engineering》2008,13(4):476-482
Microalgae perform oxygenic photosynthesis and are capable of taking up a large amount of CO2, using an inducible CO2 concentrating mechanism (CCM), and fixing CO2 into higher compounds. These characteristics make the microalgae potentially useful for removal and utilization of CO2 emitted from industrial plants and, generally, the usage of photosynthetic microorganisms has increased and significantly
improved as a solution for CO2 emissions. In this light and based on previous research using Anabaena cylindrica IAM M1 and Spirulina platensis IAM M 135, enhancement was sought for CO2 fixation and biomass production by Chlorella vulgaris Buitenzorg by increasing the photon flux density concurrent with increases in culture biomass during the cellular growth
phase and was compared to cultures of Chlorella grown at optimal constant illumination, with all cultures grown using Bennick basal medium, 29°C, and a flow of 1.0 atm.
10% CO2 enriched air delivered to three in serial photobioreactors of 0.200 dm3 capacity each. The results showed that increasing illumination during culture increased biomass production of Chlorella by ∼60% as well as increased CO2 fixation ability by ∼7.0%. It was also demonstrated that the non-competitive inhibition of [HCO3
−] as a carbon source significantly affected the cultivation in both the increasing and constant photon flux density regimes. 相似文献
15.
Summary
Leptadenia reticulata (Retz.) Wight. & Arn., an important herbal medicinal plant, belongs to the family Asclepiadaceae. This plant has been known
for its medicinal uses since 4500 BC. Presently this is an endangered species. There is a need for applying non-conventional
methods of propagation for conservation and sustainable utilization of biodiversity of Leptadenia reticulata. We developed a micropropagation method for mass multiplication of L. reticulata. Explants harvested from greenhouse-maintained and field-grown plants were used to establish cultures of L. reticulata. The nodal shoot segments were surface-sterilized and cultured on Murashige and Skoog (MS) medium along with additives (25
mg l−1 each of adenine sulfate, arginine, and citric acid, and 50 mg l−1 ascorbic acid) containing 0.6 μM indole-3-acetic acid (IAA) and 9 μM N6-benzyladenine (BA). Three to four shoots differentiated from each node within 25–30 d at 26±2°C and 36 μmol m−2 s−1 spectral flux photon (SFP) for 12 hd−1. Shoots were further multiplied by (1) repeated transfer of mother explant on fresh medium containing 0.6 μM IAA and 2.2 μM BA, and (2) subculture of in vitro-differentiated shoots on MS medium with 6.6 μM BA and 0.6 μM IAA. After three or four subcultures, the basal clump with shoot bases was divided into three or four subclumps and multiplied
on the fresh medium. From each clump 15–20 shoots regenerated within 25 d. Ninety percent of the in vitro-produced shoots rooted ex vitro if these were pulse-treated with 123 μM each of indole-3-butyric acid and β-naphthoxyacefic acid. The plantlets were transferred to bottles containing sterile ‘soilrite’
(soilless compost and soil conditioner) moistened with half-strength MS macrosalts. Ninety-five percent of the plantlets were
hardened in the bottles within 15 d. The hardened plants were then transferred to black polybags in the nursery. Field transferred
plants are growing normally and have flowered. The protocol developed is reproducible. From a single nodal segment about 1700
hardened plants could be regenerated within 174 d. 相似文献
16.
The nutrition conditions needed to redirect the carbon flux in Torulopsis glabrata, a pyruvate hyper-production yeast, from pyruvate to α-ketoglutaric acid (KG) were investigated in a stirred fermentor. A minor amount of KG (1.3 gl−1) was produced when NaOH was used to control the pH, while 12 g KG l−1 was produced when CaCO3 was used instead. When thiamine and biotin were included in the medium, 13 g KG l−1 and 68 g pyruvate l−1 were produced after 48 h when glucose was nearly consumed (approximately 5 gl−1). With fermentation continuing for a further 16 h, the concentration of pyruvate decreased to 31 gl−1, and KG increased to 30 gl−1. KG thus accumulated at the expense of pyruvate consumption.
Received 2 June 2005; Revisions requested 30 June 2005 and 1 September 2005; Revisions received 1 September 2005 and 28 October
2005; Accepted 28 October 2005 相似文献
17.
Franciane Pellizzari Eurico C. Oliveira Nair S. Yokoya 《Journal of applied phycology》2008,20(1):75-82
Gayralia K.L. Vinogr. is a monostromatic green alga of commercial importance in the southern Brazil, and its cultivation is being
considered. This paper reports some basic aspects of the biology of this poorly known genus. Two populations of Gayralia spp., from outer and inner sectors of Paranaguá Bay, showed an asexual life history with a distinct pattern of thallus ontogeny.
In one population (Gayralia sp. 1), zooids developed an expanded monostromatic blade directly, while in the other (Gayralia sp. 2) zooids produced an intermediate saccate stage, before giving rise to a monostromatic blade. Thalli of the two species
differ in size and in cell diameter. The effects of temperature (16–30°C), irradiance (50–100 μmol photons m−2 s−1), and salinity (5–40 psu) on the growth of both populations were assessed. Plantlets of Gayralia sp. 1 from in vitro cultures showed a broader tolerance to all salinity and irradiance levels tested, with the highest growth
rate (GR; mean 17% day−1) at 21.5°C and 100 μmol photons m−2 s−1. Plantlets of Gayralia sp. 1 collected during the winter in the field showed higher GR, ranging from 5% day−1 to 7.5% day−1 in salinities from 20 to 40 psu, and 2.0% day−1 and 4.3% day−1 for plantlets collected during the summer. Gayralia sp. 2 from the field showed highest GR at salinity of 15 psu. These results suggest distinct physiological responses of the
two species, in accordance with their distribution: Gayralia sp. 2 is limited to the inner areas of the estuary, while Gayralia sp. 1 grows in outer areas, where salinity values are higher than 20 psu. These data indicate that Gayralia sp. 1 has a higher potential for aquaculture than Gayralia sp. 2 due to its larger thalli, higher GR, and wider tolerance to environmental variations. 相似文献
18.
Photosynthetic photon flux density (PPFD) at 15 cm above the ground was measured at 20 microsites in gaps and grass patches
within aMiscanthus sinensis Anderss community at 10 s intervals during 5 days every month from May to September 1989.
Microsite light availability, which was characterized by daily total PPFD, sunfleck PPFD (PPFD above a threshold value of
50 or 400 μmol m−2 s−1) and the diffuse site factor, showed evident seasonal changes, with a marked reduction between June and July due to the rapid
growth of the grass canopy. The monthly median value of daily total PPFD among the microsites decreased from 10.3 mol m−2 day−1 in May to 0.77 mol m−2 day−1 in September, with a reduction in the diffuse site factor from 31 to 4%. During the summer, the median value of the total
time of sunflecks exceeding 50 μmol m−2 s−1 contributed 7–18% of measurement time, but the contribution of these sunflecks to daily total PPFD ranged from 29 to 59%.
There was considerable microsite variation in light availability throughout the measurement period. Rank correlation analysis
revealed that some microsites, such as those in gaps, consistently received more total PPFD, more sunfleck PPFD and had a
higher diffuse site factor than those in grass patches. The diffuse site factor had a linearly positive relationship with
daily total PPFD and total sunfleck PPFD for the 20 microsites during the measurement period, confirming that the diffuse
site factor is a useful index for microsite light availability withinM. sinensis canopies. 相似文献
19.
C. L. Carretero M. Cantos J. L. García A. Troncoso 《In vitro cellular & developmental biology. Plant》2007,43(4):364-369
Three cassava clones (SOM-1, “05”, and “50”) were cultured in vitro on MS medium plus sucrose (30 g L−1) and myo-inositol (100 mg L−1) without plant growth regulators and with additions of 0 (control), 0.5, 1, 1.5, 2, 2.5, and 3 g L−1 NaCl to test their salt tolerance. The same cassava clones were cultivated in greenhouse conditions on a sandy soil substratum
and irrigated with 20% strength Hoagland solution, and additions of 0, 4, and 8 g L−1 of NaCl. Salinity negatively affected the survival, development, leaf water content, and mineral composition (mainly by accumulation
of Cl and Na) of both in vitro and ex vitro plants, but with different intensity in each clone. In both conditions of culture (in vitro and ex vitro) clone SOM-1, from a desert arid saline zone of Somalia, was the most tolerant and clone “05”, from a rainy region of Ivory
Coast, the most sensitive. Clone “50” tolerance to in vitro salt treatments, although lower, was not significantly different from that of SOM-1 but the ex vitro response was similar to “05”. In general, there was a correlation between in vitro and ex vitro behavior of the cassava plant regarding salt tolerance, which would allow the in vitro culture method to be used for selection of salt-tolerant plants of this crop. 相似文献
20.
Sucrose and light effects on in vitro cultures of potato,chokecherry and saskatoon berry during low temperature storage 总被引:1,自引:0,他引:1
Pruski Kris Kozai Toyoki Lewis Tina Astatkie Tess Nowak Jerzy 《Plant Cell, Tissue and Organ Culture》2000,63(3):215-221
Cultures of potato (Solanum tuberosum) cv. Atlantic, chokecherry (Prunus virginiana L.) cv. Garrington and saskatoon berry (Amelancher alnifolia Nutt.) cv. Northline grown in vitro for 3 weeks at 24/22 °C, 16-h photoperiod, 150 μmol m−2 s−1 photosynthetic photon flux density (PPFD) mixed fluorescent/incandescent light were stored for 6, 9 and 12 weeks at 4 °C
under 0 (darkness) and 3 μmol m−2 s−1 PPFD (690 nm red light continuous illumination). Growth regulators free MSMO medium either with or without 30 g l−1 sucrose was used to store the cultures. All cultures retained capacity to re-grow after storage. Tested factors, sucrose,
light and the length of the storage period had an impact on shoot quality and re-growth capacity of the cultures. For either
light treatment sucrose was essential for the low temperature maintenance of vigorous stock plants of potato, if stored for
over 6 weeks. Chokecherry and saskatoon cultures stored well without sucrose; although chokecherry benefited from sucrose
in the storage medium when the stock cultures were kept at the low temperature for 12 weeks. Low light significantly improved
quality of the stored potato cultures, but had very little effect on both chokecherry and saskatoon berry cultures. The woody
plant cultures grew during storage, and the longer the stock plants were stored, the more vigorous cultures they generated.
The results indicate that growers can successfully use their existing facilities, small refrigerators and coolers with low
light intensity, set at 4 °C, for short term storage of potato, chokecherry and saskatoon berry cultures. The potato cultures,
which are known to be sensitive to prolonged low temperature storage, should be frequently monitored and subcultured as required.
On the other hand, the woody plant stock cultures do not require any special attention when kept at 4 °C and re-grow the most
vigorous shoots if stored for at least 12 weeks.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献