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1.
A novel bacterial strain, designated SGD-1123 T was isolated from Chorao Island, in Goa Province, India. The strain was found to be able to grow at 15–42 °C, pH 5–12 and 0–12 % (w/v) NaCl. The whole cell hydrolysates were found to contain meso-diaminopimelic acid, galactose and arabinose. The major fatty acids were identified as iso-C 15:0 and anteiso-C 15:0, MK-7 was identified as the predominant menaquinone and the predominant polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified aminolipid. The genomic DNA G+C content was determined to be 44.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences placed the isolate within the genus Bacillus and further revealed that strain SGD-1123 T had highest sequence similarity with Bacillus aquimaris, and forms a separate clade with its closest relatives i.e. B. aquimaris, Bacillus vietnamensis and Bacillus marisflavi, with which it shares 94.5, 94.1 and 94.1 % similarity respectively. The phylogenetic, chemotaxonomic and phenotypic analyses indicated that strain SGD-1123 T represents a novel species within the genus Bacillus, for which the name Bacillus enclensis is proposed. The type strain is SGD-1123 T (NCIM 5450 T=CCTCC AB 2011125 T). 相似文献
2.
A novel bacterial strain designated as NIO-1008 T was isolated from marine sediments sample in Chorao Island India. Cells of the strains were gram positive and non-motile, displayed a rod–coccus life cycle and formed cream to light grey colonies on nutrient agar. Strain NIO-1008 T had the chemotaxonomic markers that were consistent for classification in the genus Arthrobacter, i.e. MK-9(H 2) (50.3 %), as the major menaquinone, and the minor amount of MK-7 (H 2-27.5 %), MK-8 (H 4-11.6 %) and MK-8 (H 2-10.4 %). anteiso-C 15:0, iso-C 15:0, iso-C 16:0 and C 15:0 were the predominant fatty acids. Galactose, glucose and rhamnose are the cell-wall sugars, and DNA G+C content was 61.3 mol%. Phylogenetic analysis, based on 16S rRNA gene sequencing, showed that the strains were most similar to Arthrobacter equi IMMIB L-1606 T, Arthrobacter chlorophenolicus DSM 12829 T, Arthrobacter defluvii KCTC 19209 T and Arthrobacter niigatensis CCTCC AB 206012 T with 98.5, 98.4, 98.0 and 97.8 %, respectively, and formed a separate lineage. Combined phenotypic data and DNA–DNA hybridization data supported the conclusion that strains NIO-1008 T represent a novel species within the genus Arthrobacter, for which the name Arthrobacter enclensis sp. nov., is proposed. The type strain is NIO-1008 T = (NCIM 5488 T = DSM 25279 T). 相似文献
4.
A Gram-positive, pinkish-orange pigmented, coccoid strain, FCS-11 T was isolated from a marine sediment sample taken from Kochi fort area, Kerala, India and subjected to polyphasic taxonomic
study. The 16S rRNA gene sequence of the strain was determined and the results of 16S rRNA gene sequence analysis showed that
the strain FCS-11 T should be assigned to the genus Kocuria. The chemotaxonomic data supported this taxonomic placement i.e. menaquinones MK-7(H 2), MK-8(H 2) and MK-9(H 2); major fatty acids anteiso C15:0 and iso-C15:0 and phosphatidylglycerol (PG) and diphosphatidylglycerol (DPG) as major polar
lipids. Further phylogenetic analysis of the 16S rRNA gene sequence confirmed that the strain FCS-11 T belonged to the genus Kocuria and is closely related to Kocuria turfanensis MTCC 10790 T (99.4%) followed by Kocuria polaris MTCC 3702 T (98.2%), Kocuria rosea MTCC 2522 T (98.2%), Kocuria flava MTCC 10971 T (98.2%), Kocuria aegyptia MTCC 10791 T (98.0%), Kocuria himachalensis MTCC 7020 T (97.5%) and Kocuria atrinae MTCC 10972 T (97.1%). However, the DNA–DNA hybridisation values obtained between strain FCS-11 T and other related strains were well below the threshold that is required for the proposal of a novel species. The G+C content
of the genomic DNA was 60.7 mol%. The phenotypic and genotypic data showed that the strain FCS-11 T merits the recognition as a representative of a novel species of the genus Kocuria. It is proposed that the isolate should be classified in the genus Kocuria as a novel species, Kocuria sediminis sp. nov. The type strain is FCS-11 T (= MTCC 10969 T = JCM 17929 T). 相似文献
7.
A novel Gram-stain-negative, rod-to-spiral-shaped, oxidase- and catalase- positive and facultatively aerobic bacterium, designated HS6 T, was isolated from marine sediment of Yellow Sea, China. It can reduce nitrate to nitrite and grow well in marine broth 2216 (MB, Hope Biol-Technology Co., Ltd) with an optimal temperature for growth of 30–33 °C (range 12–45 °C) and in the presence of 2–3 % (w/v) NaCl (range 0.5–7 %, w/v). The pH range for growth was pH 6.2–9.0, with an optimum at 6.5–7.0. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that the novel isolate was 93.3 % similar to the type strain of Neptunomonas antarctica, 93.2 % to Neptunomonas japonicum and 93.1 % to Marinobacterium rhizophilum, the closest cultivated relatives. The polar lipid profile of the novel strain consisted of phosphatidylethanolamine, phosphatidylglycerol and some other unknown lipids. Major cellular fatty acids were summed feature 3 (C 16:1 ω7c/iso-C 15:0 2-OH), C 18:1 ω7c and C 16:0 and the main respiratory quinone was Q-8. The DNA G+C content of strain HS6 T was 61.2 mol %. Based on the phylogenetic, physiological and biochemical characteristics, strain HS6 T represents a novel genus and species and the name Motiliproteus sediminis gen. nov., sp. nov., is proposed. The type strain is HS6 T (= ATCC BAA-2613 T=CICC 10858 T). 相似文献
10.
A novel Gram-positive strain, designated NIO-1003 T, was isolated from a marine sediment sample collected from the Chorao Island, Goa Provence, India. Strain NIO-1003 T was found to be strictly aerobic, motile, endospore-forming rods. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NIO-1003 T belongs to the genus Fictibacillus and to be most closely related to Fictibacillus rigui KCTC 13278 T, Fictibacillus solisalsi KCTC 13181 T and Fictibacillus barbaricus DSM 14730 T with 98.2, 98.0 and 97.2 % similarity and 25, 28, 39 nucleotide differences respectively. Strain NIO-1003 T was characterized by having cell-wall peptidoglycan based on meso-diaminopimelic acid and MK-7 as the predominant menaquinone. The polar lipid profile exhibited the major compounds diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. In addition, minor amounts of an aminophospholipid were detected. The major fatty acids were identified as ai-C 15:0, iso-C 15:0, ai-C 17:0 and C 16:0, supporting the grouping of strain NIO-1003 T into the family Bacillaceae. The DNA G+C content of strain NIO-1003 T was determined to be 42.6 mol%. On the basis of phenotypic properties, phylogeny and DNA–DNA hybridisation analysis, strain NIO-1003 T is considered to represent a novel species of the genus Fictibacillus for which the name Fictibacillus enclensis sp. nov. is proposed. The type strain is NIO-1003 T (= NCIM 5458 T = DSM 25142 T). 相似文献
11.
A Gram stain-negative, aerobic and rod-shaped bacterium, strain DY22 T, was isolated from a deep-sea sediment collected from the east Pacific Ocean. The isolate was found to grow in the presence of 0–20.0 % (w/v) NaCl and at pH 4.5–8.5; optimum growth was observed with 0.5–2.0 % (w/v) NaCl and at pH 5.0–7.0. Chemotaxonomic analysis showed the presence of ubiquinone-9 as predominant respiratory quinone and C 16:0, C 19:0 ω8 c cyclo and C 12:0 3-OH as major cellular fatty acids. The genomic DNA G+C content was determined to be 59.6 mol%. Comparative 16S rRNA gene sequence analysis revealed that the novel isolate belongs to the genus Salinicola. Strain DY22 T exhibited the closest phylogenetic affinity to the type strain of Salinicola salarius with 97.2 % sequence similarity and less than 97 % sequence similarity with respect to other Salinicola species with validly published names. The DNA–DNA reassociation values between strain DY22 T and S. salarius DSM 18044 T was 52 ± 4 %. On the basis of phenotypic, chemotaxonomic and genotypic data, strain DY22 T represents a novel species of the genus Salinicola, for which the name Salinicola peritrichatus sp. nov. (type strain DY22 T = CGMCC 1.12381 T = JCM 18795 T) is proposed. 相似文献
13.
A Gram-staining-negative, aerobic and pear-shaped bacterial strain, designated WL0036T, was isolated from coastal sediment sample collected in Nantong city, Jiangsu province of China (120° 51′ 13″ E, 32° 6′ 26″ N) in October 2020. Strain WL0036T was found to grow at 20–37 °C (optimum, 28 °C) with 0–9.0% NaCl (optimum, 2.5–4.0%) and displayed alkaliphilic growth with the pH range of pH 6.0–10.0 (optimum, pH 7.0–8.0). The polar lipids profile of strain WL0036T included phosphatidylcholine, phosphatidylethanolamine, glycolipid and an unidentified lipid. The major isoprenoid quinone was determined to be Q-11 and the major fatty acids were C16:0, 11-methyl-C18:1ω7c, and summed features 8 (C18:1ω6c and/or C18:1ω7c). The G?+?C content of genomic DNA was 61.8%. Phylogenetic trees constructed based on 16S rRNA gene sequence and bac120 gene set (a collection of 120 single-copy protein sequences prevalent in bacteria) indicted that strain WL0036T clustered with strains Hyphomonas neptunium ATCC 15444T and H. polymorpha PS728T. The average nucleotide identities between strain WL0036T and strains H. neptunium ATCC 15444T and H. polymorpha PS728T were 80.7% and 81.2%, respectively. Strain WL0036T showed 22.8% and 23.2% of digital DNA-DNA hybridization identities with H. neptunium ATCC 15444T and H. polymorpha PS728T, respectively. As inferred from the phenotypic and genotypic characteristics and the phylogenetic trees, strain WL0036T ought to be recognized as a novel species in genus Hyphomonas, for which the name Hyphomonas sediminis sp. nov. is proposed. The type strain is WL0036T (=?MCCC 1K05843T?=?JCM 34658T?=?GDMCC 1.2413T). 相似文献
14.
A taxonomic study was carried out on strain D104 T, which was isolated from deep-sea subsurface sediment sample from the Arctic Ocean. The bacterium was found to be Gram-negative, oxidase negative and catalase weakly positive, rod shaped, motile by means of polar flagellum. The organism grows between 4 and 37 °C (optimum 25–28 °C) and 0.5–6 % NaCl (optimum 3 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain D104 T belongs to the genus Marinomonas, with highest sequence similarities of 97.7 % to Marinomonas ushuaiensis DSM 15871 T, followed by M. dokdonensis DSW10-10 T (96.9 %), M. arenicola KMM 3893 T (96.7 %), M. arctica 328 T (96.6 %) and other 18 species of the genus Marinomonas (94.4–96.5 %). The average nucleotide identity and estimated DNA–DNA hybridization values between strain D104 T and M. ushuaiensis DSM 15871 T were 84.24 % and 20.80 ± 2.33 % respectively. The principal fatty acids were C 16:0, sum in feature 3 (C 16:1 ω7 c/C 16:1 ω6 c), sum in feature 8 (C 18:1 ω7 c/C 18:1 ω6 c) and C 12:1 3OH. The G + C content of the chromosomal DNA was determined to be 44.8 mol%. The respiratory quinone was found to be Q8 (100 %). Polar lipids include phosphatidylglycerol and phosphatidylethanolamine as major phospholipids and aminolipid and phospholipid as minor components. The results of the genotypic and phenotypic analyses indicate that strain D104 T represents a novel species of the genus Marinomonas, for which the name Marinomonas profundimaris sp. nov. is proposed, with the type strain D104 T (=MCCC 1A07573 T = LMG 27696 T). 相似文献
15.
A Gram-positive, rod-shaped bacterium, designated strain SCSIO N0306 T, was isolated from an abyssal sediment sample collected from the Indian Ocean. The isolate was found to grow optimally at 0–2 % (w/v) NaCl, pH 7.0 and 30 °C. Comparative analysis of the 16S rRNA gene sequence showed that the isolate SCSIO N0306 T belongs phylogenetically to the genus Paenibacillus, and to be most closely related to P. algorifonticola XJ259 T (with 95.47 % sequence similarity), sharing less than 95.0 % sequence similarity with all other taxa of this genus. Chemotaxonomic analysis revealed MK-7 as the major isoprenoid quinone, the DNA G+C content was determined to be 45.5 mol%, and anteiso-C 15:0, C 16:0, and iso-C 15:0 were identified as the major fatty acids. On the basis of this polyphasic taxonomic data, isolate SCSIO N0306 T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus abyssi sp. nov. is proposed. The type strain is SCSIO N0306 T (= DSM 26238 T = CGMCC 1.12987 T). 相似文献
18.
A Gram-positive bacterium, designated SCSIO 15042 T, was isolated from a sediment of the South China Sea and was subjected to a polyphasic taxonomic study. The isolate grew at 20–60 °C, pH 6.0–10.0 and it could grow with up to 10 % (w/v) NaCl. The cell-wall diamino acid was found to be meso-diaminopimelic acid. Polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine and an unknown polar lipid. The only menaquinone was determined to be MK-7. The major fatty acids were identified as C 16:1 ω7 c/C 16:1 ω6 c, C 16:0, iso-C 15:0, anteiso-C 15:0, and iso-C 16:0. The DNA G+C content of strain SCSIO 15042 T was determined to be 43.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain SCSIO 15042 T to the genus Bacillus. Levels of 16S rRNA gene sequence similarities between strain SCSIO 15042 T and Bacillus herbersteinensis D-1-5a T, Bacillus infantis SMC 4352-1 T, Bacillus novalis LMG 21837 T and Bacillus drentensis LMG 21831 T were 96.2, 96.2, 96.1 and 96.1 %, respectively. Based on the evidence of the present polyphasic study, strain SCSIO 15042 T is considered to represent a novel species of the genus Bacillus, for which the name Bacillus abyssalis sp. nov. is proposed. The type strain is SCSIO 15042 T (=DSM 25875 T = CCTCC AB 2012074 T = NBRC 109102 T). 相似文献
20.
A Gram-positive, rod-shaped, endospore-forming organism, strain BL3-6 T, was isolated from tidal flat sediments of the Yellow Sea in the region of Tae-An. A 16S rRNA gene sequence analysis demonstrated
that this isolate belongs to the Bacillus cereus group, and is closely related to Bacillus mycoides (99.0% similarity), Bacillus thuringiensis (99.0%), Bacillus weihenstephanensis (99.0%), Bacillus cereus (98.9%), Bacillus anthracis (98.8%), and Bacillus pseudomycoides (98.1%). The phylogenetic distance from any validly described Bacillus species outside the Bacillus cereus group was less than 95.6%. The DNA G+C content of the strain was 39.4 mol% and the major respiratory quinone was menaquinone-7.
The major cellular fatty acids were iso-C 14:0 (17.8%), iso-C 16:0 (15.8%), and iso-C 12:0 (11.3%). The diagnostic amino acid of the cell wall was meso-diaminopimelic acid and the major cell wall sugar was galactose. The results of DNA-DNA hybridization (<55.6%) and physiological
and biochemical tests allowed genotypic and phenotypic differentiation of strain BL3-6 T from the published Bacillus species. BL3-6 T therefore represents a new species, for which the name Bacillus gaemokensis sp. nov. is proposed, with the type strain BL3-6 T (=KCTC 13318 T =JCM 15801 T). 相似文献
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