胸腺五肽联合干扰素治疗慢性湿疹的疗效观察

目的：探讨胸腺五肽联合干扰素治疗慢性湿疹的临床效果及安全性。方法：随机将2009-2012年我科收治的60例慢性湿疹患者分为两组,治疗组30例在给予相应的抗组胺药、非特异性抗过敏治疗的基础上加用胸腺五肽针10mg与干扰素α-2b200万u交替肌注,对照组30例只给予抗组胺药、非特异性抗过敏治疗,两组均在3周后观察其治疗效果及不良反应的发生情况。结果：治疗三周后,治疗组总有效率为70％,对照组总有效率为33．33％,两组总有效率比较差异有统计学意义（P〈0．05）。两组不良反应的发生率比较并无统计学差异（P〉0．05）。结论：胸腺五肽联合干扰素治疗慢性湿疹的临床疗效显著,副作用较小,安全性较高,值得临床推广应用。     

胸腺五肽显著增强干扰素在抗病毒治程中的特异性CTL效应

目的:探究在e抗原(HBe Ag)阳性的慢性乙型肝炎患者采用聚乙二醇干扰素-2a(peg-2a)联合核苷类药物治疗过程中,加用胸腺五肽对细胞免疫应答的影响。方法:选择采用聚乙二醇干扰素α-2a联合核苷类药物(拉米夫定+阿德福韦酯)治疗48周,HBe Ag仍为阳性,且HLA-A2阳性的慢性乙型肝炎患者18例,分为两组。一组原方案延长联合治疗作为对照,另一组在原方案基础上再加用胸腺五肽治疗(10 mg/次,2次/周,共24周)治疗,所有病人均治疗至96周。并做体外HBV特异性细胞毒T淋巴细胞(HBV specific CTL)培养增殖,通过Elispot技术分析其分泌细胞因子(肿瘤坏死因子-α,干扰素-γ,白介素-10)的功能。结果:HBe Ag转阴率,治疗96周时联合胸腺五肽组为44.4%(4/9),原方案对照组为22.2%(2/9)。HBs Ag滴度,48周时,HBs Ag为4571±3772 IU/m:;96周时,联合胸腺五肽组为1962±2869 IU/m L,转阴1人,原方案对照组为3490±3124 IU/m L,P=0.093。HBV特异性CTL培养增殖,96周时联合胸腺五肽组高于原方案对照组,且联合胸腺五肽组TNF-的分泌也高于原方案对照组,P0.05。结论:胸腺五肽显著增强干扰素抗病毒治疗过程中的特异性CTL效应。     

丹皮酚软膏治疗手部慢性湿疹疗效观察

目的:评估丹皮酚软膏治疗手部慢性湿疹的临床疗效及安全性。方法:采用4周对照试验,第1、2、4周复诊,临床疗效评价及不良反应判定。结果:观察组37例,对照组36例。4周后总有效率分别为89.19%、91.67%,组间有效率差异无统计学意义。第1周评分差异有统计学意义,第2、4周总评分差异无统计学意义。结论:丹皮酚软膏治疗手部慢性湿疹2周后临床疗效与派瑞松相当,安全性好。在减轻脱屑及皲裂方面优于派瑞松。     

夫西地酸乳膏联合扑尔敏及肤痔清软膏治疗婴幼儿湿疹疗效观察

目的观察夫西地酸乳膏联合扑尔敏及肤痔清软膏治疗婴幼儿湿疹的效果。方法治疗组首先使用夫西地酸乳膏,2次/d,连用1周,然后使用肤痔清软膏,2次/d,连用1周;对照组使用肤痔清软膏,2次/d,连用2周。两组均使用相同的抗组胺药扑尔敏口服止痒。结果治疗组63例中有56例可评价疗效,治愈率为64.29%,有效率为85.71%;对照组63例中有54例可评价疗效,治愈率为37.04%,有效率为61.11%。两组有效率比较,差异有统计学意义(χ2=7.02,P0.05)。两组中均无不良事件。结论夫西地酸乳膏联合扑尔敏及肤痔清软膏治疗婴幼儿湿疹疗效好,无明显不良反应。     

VEGF 对大鼠脑缺血再灌注损伤的预防作用及其机制研究

目的:观察复方甘草酸苷联合抗组胺类药物治疗慢性荨麻疹的临床疗效.方法:150例慢性荨麻疹患者随机分为治疗组和对照组,治疗组口服复方甘草酸苷片和西替利嗪片,对照组只给予西替利嗪片,疗程14d.评价1周和2周时的荨麻疹活动度评分表和临床疗效.结果:治疗1周末和2周末两组的荨麻疹活动度评分都明显下降,与治疗前比较差异具有统计学意义(P＜0.05),两组1周末和2周末时比较差异具有统计学意义(P＜0.05).1周末两组总有效率分别为64.0％和38.7％,差异具有统计学意义(P＜0.05);2周末总有效率分别为90.7％和61.3％,差异具有统计学意义(P＜0.05).结论:复方甘草酸苷联合西替利嗪片治疗慢性荨麻疹具有起效快、疗效高的特点.     

CO2 激光联合胸腺五肽治疗尖锐湿疣的临床效果分析

目的:探讨CO2激光联合胸腺五肽治疗尖锐湿疣的临床疗效。方法:将我院2010年2月-2011年8月期间收治的82例尖锐湿疣患者随机分为两组,治疗组50例采用CO2激光器治疗联合胸腺五肽肌注;对照组32例单纯采用CO2激光器治疗;两组治疗疗程结束后观察临床疗效。结果:治疗组50例,一次治愈者46例,占92%;二次治愈者4例,占8%。对照组32例,一次治愈者19例,占59.4%;二次治愈者13例,占40.6%。两组一次治愈率比较差异明显,具有统计学意义(P<0.05)。两组术后局部轻度水肿和疼痛,无需处理1~3天缓解或痊愈。结论:CO2激光联合胸腺五肽治疗尖锐湿疣临床疗效好,并具有治疗简便,术后不良反应少,治愈率高等特点。     

CO_2激光联合胸腺五肽治疗尖锐湿疣的临床效果分析

目的：探讨CO2激光联合胸腺五肽治疗尖锐湿疣的临床疗效。方法：将我院2010年2月-2011年8月期间收治的82例尖锐湿疣患者随机分为两组,治疗组50例采用CO2激光器治疗联合胸腺五肽肌注;对照组32例单纯采用CO2激光器治疗;两组治疗疗程结束后观察临床疗效。结果：治疗组50例,一次治愈者46例,占92%;二次治愈者4例,占8%。对照组32例,一次治愈者19例,占59.4%;二次治愈者13例,占40.6%。两组一次治愈率比较差异明显,具有统计学意义（P〈0.05）。两组术后局部轻度水肿和疼痛,无需处理1~3天缓解或痊愈。结论：CO2激光联合胸腺五肽治疗尖锐湿疣临床疗效好,并具有治疗简便,术后不良反应少,治愈率高等特点。     

微型钛板联合颌间牵引钉内固定术治疗颌骨骨折疗效观察

目的:探讨微型钛板联合颌间牵引钉内固定术治疗颌骨骨折的临床效果及其在临床的安全性。方法:随机选取颌骨骨折患者共60例,将上述患者随机平均分成对照组与观察组。分别给予与单纯微型钛板坚固内固定治疗和微型钛板联合颌间牵引钉内固定术治疗方法。分析比较这两组患者4周内的临床治疗效果及安全性。结果:术后4周两组间临床治疗效果比较显示:采取微型钛板联合颌间牵引钉内固定术治疗的观察组治疗效果明显好于给予单纯微型钛板坚固内固定治疗的对照组。观察组总有效率为87%,对照组总有效率为53.3%,差异有统计学意义(P0.05).两组手术过程均顺利实施,患者术后均无严重并发症发生,术后恢复情况较好,经比较两组术后不良反应的差异无统计学意义(P0.05)。结论:微型钛板联合颌间牵引钉内固定术是治疗颌骨多发骨折有效安全方法。     

胃复春联合三联抗幽门螺杆菌治疗慢性萎缩性胃炎合并消化性溃疡的疗效分析

目的:探讨胃复春联合三联抗幽门螺杆菌(HP)治疗慢性萎缩性胃炎(CAG)合并消化性溃疡(PU)的有效性及安全性.方法:将104例HP阳性的CAG合并PU患者随机分为治疗组和对照组,每组各52例.所有患者均采用三联抗HP疗法:奥克+阿莫西林胶囊+克拉霉素,2周后单独服用奥克,连用4周;治疗组在此基础上加用胃复春治疗,连用4周.观察患者症状改善情况、HP根除率、1年复发率、胃黏膜的慢性炎症程度及治疗期间的不良反应.结果:治疗后,治疗组CAG和PU的总有效率分别为94.2％和92.3％,均显著高于对照组总有效率80.8％和75.0％(P＜0.05);联合组HP根除率和1年复发率为86.5％和15.6％,三联组分别为67.3％和37.1％,两组比较差异均有统计学意义(P＜0.05);治疗后,两组胃黏膜慢性炎症程度评分均较治疗前显著提高,但治疗组升高程度较对照组更为显著(P＜0.05);两组均未见严重不良反应.结论:胃复春联合三联抗HP治疗CAG合并PU疗效优于单用抗HP治疗,提高HP的根治率,降低复发率,且毒副作用少.     

氟芬那酸丁酯软膏联合丁酸氢化可的松治疗儿童慢性湿疹及特应性皮炎的疗效

目的:探讨氟芬那酸丁酯软膏联合丁酸氢化可的松治疗儿童慢性湿疹及特应性皮炎的临床疗效。方法:选取我院诊治的110例慢性湿疹及特应性皮炎患儿作为研究对象,使用随机数字表法分为研究组和对照组(各55例),对照组单用丁酸氢可的松软膏治疗,研究组联合应用氟芬那酸丁酯软膏和丁酸氢化可的松软膏,比较两组患儿的治疗效果。结果:研究组治疗后的疾病积分显著低于对照组,P0.05;研究组的治疗总有效率为85.45%,对照组为63.64%,组间比较,差异具有统计学意义(P0.05)。结论:临床治疗儿童慢性湿疹和特应性皮炎,联合应用氟芬那酸丁酯软膏和丁酸氢化可的松,具有显著疗效,且安全性好,值得推广。     

Characteristics of the membranes of the pellicle of the ciliatePseudomicrothorax dubius

Summary The membranes of the pellicle of the ciliatePseudomicrothorax dubius are investigated using thin section electron microscopy and freeze-fracture replicas. The plasma membrane is covered by a surface coat and is connected to the outer alveolar membrane by short, sometimes branched, bridges. The inner alveolar membrane is coated on both sides. The epiplasm lies in intimate contact with the cytoplasmic surface of this membrane, and there is a corresponding deposit on the other surface. This deposit is regularly striated.The epiplasmic layer and the alveoli are interrupted at sites of cytotic activity,e.g., the attachment sites of trichocysts, the cytoproct, and the parasomal sacs. The striated deposit ends where the epiplasm ends, indicating a direct relationship between these two epimembranous layers.There is a deposit along the sides of the first part of the tip of the trichocysts, and in this region the trichocyst membrane is free of intramembranous particles.The membrane of the parasomal sacs has a coat on both surfaces. That on the extraplasmic surface is similar to the surface coat of the plasma membrane. The origin of the cytoplasmic coat is unknown. The cytotic activity of these sacs is indicated by their highly irregular profiles.     

The early development of the tail and the transformation of the shape of the nucleus of the spermatid of the domestic fowl,Gallus gallus

Summary The differentiation of the spermatid, especially in reference to the formation of the flagellum, and transformation of the shape of the nucleus was investigated in the domestic fowl.In the early stage of the spermatid, a prominent Golgi apparatus appears around the centrioles. The Golgi vesicles then surround the axial-filament complex which develops from the distal centriole. These vesicles fuse to form continuous membrane at the earliest stage of flagellar formation, and in the succeeding stage Golgi lamellae are attached to the plasma membrane of the developing flagellum. From these observations, it is assumed that Golgi apparatus may be a source of the membrane system of the flagellum.The microtubules distributed around the nucleus form the circular manchette. The anterior region of the nucleus with the manchette is cylindrical in shape and the posterior region without it remains irregular in shape. When the circular manchette has been completed, the whole nucleus acquires a slender cylindrical shape. The circular manchette then changes into the longitudinal manchette. The nuclei of spermatids without a longitudinal manchette are abnormal in shape. In view of these observations it is assumed that the nuclear shaping of the spermatid may be accomplished by circular manchette and the maintenance of shape of the elongated nucleus by longitudinal manchette.The authors wish to thank Mr. Takayuki Mori for his helpful suggestions and technical advices     

Characterization of the composition of the aqueous humor and the vitreous body of the eye of the frog Rana temporaria L

This study aimed to analyze the aqueous humor (AH) and the vitreous body (VB) of the eye of the adult frog Rana temporaria L. as a representative species of amphibians, which lead a semi-terrestrial life. The presence of collagen, albumin, uric acid and electron donors was shown in both media; however, there are slight differences in their concentrations. To determine collagen, a spectral-fluorescent probe, cyanine dye, was used. The presence of collagen in AH of the frog was found at the first time. The total content of electron donors (ascorbic and uric acids, tryptophan, and tyrosine) in VB and HA was roughly estimated at ~ 1.5 × 10^− 4 mol/L. Both VB and AH absorb light in similar UV regions. The total protein and albumin contents in AH were found to be somewhat higher than those in VB. The uric acid content was at an equally low level in both intraocular media. It is supposed that the similarity of VB and AH compositions shown in this work is due to some exchange between VB and AH contents in the course of accommodation. The role of intraocular fluids in physiological functions of the eye and in protecting the retina against UV light is discussed.     

Observations on the permeability of the choriocapillaris of the eye

Summary The choriocapillaris is a fenestrated capillary bed located posterior to the retinal pigment epithelium. It serves as the main source of supply to the photoreceptors, retinal pigment epithelium, and other cells of the outer retina. The permeability of these capillaries to intravenously injected ferritin (MW — approx. 480,000; mol. diam. 11 nm) was examined in the mouse, rabbit, and guinea pig, each of which is characterized by a different type of retinal vascularization. In all three species, the bulk of the ferritin remained in the capillary lumina, where it appeared to be blocked at the level of the diaphragmed fenestrae. Some ferritin was present in endothelial cell vacuoles. The results confirm previous work on the rat choriocapillaris and indicate that the barrier function of the choriocapillary endothelium is present even among species in which the retinal circulation differs significantly.Supported by NIH grant EY03418