假鞭叶铁线蕨孢子的组织培养

1植物名称假鞭叶铁线蕨(Adiantum malesianum Ghatak)。2材料类别成熟孢子。3培养条件孢子萌发培养基:1/2MS 30g·L-1蔗糖。原叶体增     

马齿苋的组织培养与快速繁殖

马齿苋又名长命菜 ,长寿菜 ,为马齿苋科 1年生肉质草本植物。全草入药 ,具清热、凉血、解毒之功效 ,用于湿热泄泻、疔疮肿毒、蛇虫咬伤、痔疮肿痛、湿疹等。据现代医学研究报道 ,马齿苋中含有较高浓度的去甲肾上腺素 (每 g鲜品含量达 2 .5 mg) ,对糖尿病具有食疗作用 ;含有 ω- 3脂肪酸 ,对心血管有保护作用 ;含有较丰富的铜元素 (每 g干品含 2 1μg) ,可作为白癜疯患者和因缺铜元素而造成白发的患者的辅助食疗菜肴 ;还含有大量的钾盐 ,对维持心肌功能、参与细胞新陈代谢 ,维持渗透压 ,维持神经肌肉正常功能等 ,具有良好的保健作用。作为野…     

管花蒲公英的组织培养

1植物名称管花蒲公英(Neo-Taraxacum siphonanthum). 2材料类别种子繁殖后长出幼苗,取其叶片和叶脉.     

岩芋的组织培养

1植物名称岩芋(Remusatia vivipara Schott). 2材料类别珠芽. 3培养条件(1)丛芽诱导培养基:MS 6-BA 4 mg·L-1(单位下同) 2iP 4 NAA 0.5;(2)丛芽增殖培养基:MS 6-BA 4;(3)生根培养基:MS NAA 0.5.培养基中蔗糖浓度为3%,琼脂固化,pH 5.8.培养温度(27±2)℃,光照时间12 h·d-1 ,光照度2000 1x.     

龙葵叶的组织培养

1植物名称龙葵(Solanum nigrum L.). 2材料类别幼叶.     

金缕梅的组织培养

1植物名称金缕梅(Hamamelis mollis O1iv.). 2材料类别浙江龙王山自然保护区采收的当年健康成熟的种子. 3培养条件种子萌发培养基:(1)MS0;诱导培养基:(2)MS 6-BA 1.0 mg·L-1 (单位下同) NAA 0.1 2,4-D 0.1;增殖培养基:(3)MS 6-BA 0.2 NAA0.1,(4)MS 6-BA 1.0 NAA 0.1;壮苗培养基:(1)MS0;生根培养:(5)1/2MS KT 0.1 NAA 0.6,(6)1/2MS 6-BA 0.1 NAA 1.0.培养基(1)～(4)附加蔗糖30 g·L-1,(5)和(6)附加蔗糖20 g·L-1 ,琼脂为6.5 g·L-1 ,pH 5.8.培养温度(25±1)℃,种子萌发培养和生根培养的前期进行暗培养,其余阶段均照光,光照时间12 h·d-1 ,光照度为2 500～3 000 1x.     

广东石斛的组织培养

1植物名称广东石斛(Dendrobium wilsonii Rolfe). 2材料类别幼芽.     

大花细辛的组织培养

1植物名称大花细辛(Asarum maximum Hemsl.). 2材料类别茎段. 3培养条件基本培养基为MS.芽增殖培养基:(1)MS 6-BA 1.0～2.0 mg·L-1(单位下同) NAA 0.1;壮苗培养基:(2)MS 6-BA 0.5 NAA 0.1;生根培养基:(3)1/2MS IBA 1.0 NAA 0.2.以上培养基蔗糖浓度(1)和(2)为3.0%,(3)为2.0%;琼脂7.0g·L-1,pH 5.4～5.6.培养温度为(25±2)℃,连续光照12 h·d-1,光强40μmol·m-2·s-1.     

海南龙血树的组织培养

1植物名称海南龙血树(Dracaena cambodiana). 2材料类别幼嫩茎段. 3培养条件(1)诱导培养基:MS 6-BA 5.0 mg·L-1(单位下同) NAA 0.5 Ad(腺嘌呤)40;(2)增殖培养基:MS 6-BA 5.0 NAA 0.5 Ad 0.4;(3)分化壮苗培养基:MS 6-BA 5.0 NAA 0.1 Ad 40 AC(活性碳)1 g·L-1;(4)生根培养基:MS NAA 0.3 AC 1 g·L-1.以上培养基均添加30 g·L-1蔗糖、5.8g·L-1卡拉胶,pH 6.0.培养温度26～28℃,光照时间8～10 h·d-1,光照度1500～2000 1x.     

桃儿七的组织培养

1 植物名称 桃儿七[Sinopodophyllum emodi（Wall.）Ying]。     

Factors affecting genetic transformation and shoot organogenesis of Bacopa monnieri (L.) Wettst

Efficient Agrobacterium tumefaciens mediated T-DNA delivery and subsequent shoot organogenesis has been achieved from Bacopa monnieri. Various factors influenced T-DNA delivery as evident from transient GUS assay. The transient GUS expression was significantly higher (97.7 %) in explants that were pre-cultured before bacterial infection on medium supplemented with 100 μM acetosyringone. Incorporation of acetosyringone into the co-cultivation medium also enhanced transient GUS activity. Explant injury with carborundum paper, co-cultivation period of 2 days and a bacterial density of 0.4 OD₆₀₀ showed higher transient GUS expression. Following co-cultivation, shoot organogenesis was achieved from leaf segments on basal Murashige and Skoog medium containing 58 mM sucrose. Supplementation of antibiotics (cefotaxime or carbenicillin) at > 250 μg/ml into the medium significantly promoted shoot organogenesis from leaf explants (71.5 % in control and > 83.0 % on medium containing 500 μg/ml of carbenicillin or cefotaxime). Stable transformation of regenerated shoots was confirmed on the basis of GUS activity and PCR amplification of DNA fragments specific to reporter gene (uidA) and selection marker gene (nptII). The expression level of nptII gene in independent transgenic lines was studied using quantitative real time-PCR. Stable transformed shoots after rooting were successfully established in the pots.     

Diversity among wild accessions of Bacopa monnieri (L.) Wettst. and their morphogenetic potential

Biochemical and molecular diversity among 14 accessions of Bacopa monnieri (L.) Wettst. collected from various locations of India was investigated. A significant variation was recorded in bacoside A contents of these accessions. A scatter plot of principle component analysis based on bacoside A contents clubbed these populations into two major groups and accession BM14 was placed separately. Similarly, about 35 % variations were detected in these populations based on combined data of random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR). Individually, ISSR markers detected higher variation (44.9 %) as compared to RAPD markers (23 %). Clustering based on molecular marker data grouped these accessions into two major groups and also placed accession BM14 as an out group. The shoot organogenic potential of leaf explants taken from microshoots and rooting of microshoots also varied among accessions. Maximum shoot organogenic potential was observed in accession BM5 and maximum rooting potential was observed in accessions BM1, BM2, BM7, BM10 and BM14. Present study is an important step for developing long-term strategy for conservation of this important medicinal herb.     

Direct somatic embryogenesis and encapsulation of somatic embryos for in vitro conservation of Bacopa monnieri (L.) Wettst

Direct somatic embryogenesis and shoot organogenesis were achieved from leaf explants excised from microshoots of Bacopa monnieri cultured on Murashige and Skoog medium containing N6-benzyladenine (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D). The maximum frequency of explants differentiated somatic embryos and shoot buds on MS medium supplemented with 12.5 µM BA and 1 µM 2,4-D. The frequency of explants differentiating somatic embryos decreased with increasing concentration of 2,4-D. Light and scanning electron microscopy revealed direct differentiation of somatic embryos and shoot buds from explants, and various developmental stages of the somatic embryos were observed. Somatic embryos and apical shoot tips were encapsulated in sodium alginate gel to produce synthetic seeds. The storage of synthetic seeds produced by encapsulation was studied at 4 and 25?°C (room temperature) for a period of 140 days. Encapsulated somatic embryos were found to retain viability after 140 days of storage at both temperatures, whereas encapsulated apical shoot buds failed to germinate even after 40 days when stored at 4?°C. The viability of synthetic seeds was higher when stored at 25?°C. All amplified markers scored by random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) were monomorphic for all the plants produced from synthetic seeds following different periods of storage, thus establishing the clonal fidelity of propagated plantlets.     

Effect of biologically synthesized silver nanoparticles on Bacopa monnieri (Linn.) Wettst. plant growth metabolism

In this present study, interactions of biologically synthesized silver nanoparticles on hydroponically grown Bacopa monnieri (Linn.) Wettst. plant growth metabolism were documented. Estimates of protein, carbohydrate, total phenols, in addition antioxidant enzymes, catalase and peroxidise were assayed in various parts of the plants grown in hydroponic solution. The silver nanoparticles used in this study were synthesized by treating AgNO₃ with aqueous leaves extracts of Acalypha indica Linn., a medicinal herb as a source of reductants. Enhanced peroxidase and catalase activity, simulated the stress conditions induced by the silver nitrate treatment. No severe toxic effects were observed in silver nanoparticles treated plants in the morphological studies under scanning electron microscopy (SEM) while structural aberrations were observed in the light microscopic evaluation of root and stem anatomy. Further, the uptake of silver in the root and stem tissues of B. monnieri (Linn.) Wettst. was confirmed using atomic absorption spectrophotometer (AAS).     

Influence of explant types,non-embryogenic synseed and reduced oxygen environment on in vitro conservation of Bacopa monnieri (L.) Wettst

In Vitro Cellular & Developmental Biology - Plant - The effect of using encapsulated shoot tips (ST) and nodal segment (NS) explants, in combination with mineral oil (MO) overlay, for in vitro...     

Effects associated with insertion of rol genes on morphogenic potential in explants derived from transgenic Bacopa monnieri (L.) Wettst

Plant Cell, Tissue and Organ Culture (PCTOC) - The present study deals with the establishment of rolA-transgenic and rolB-transgenic plants for the first time through Agrobacterium tumefaciens...     

In Vitro Clonal Propagation and Medium Term Conservation of Brahmi [Bacopa monnieri (L) Wettst]

A protocol has been developed for in vitro clonal propagation leading to conservation of Bacopa monnieri (L) Wettst, a medicinal plant of high commercial potential with legendary reputation as a memory vitalizer. Single node explants when cultured on Murashige and Skoog’s medium supplemented with BA (0.2 mg l^-1), showed active shoot proliferation (22.2 shoots/ explant in 8 weeks) without callus formation. Rooting was achieved on the same medium. The generated shoots could also be conserved for 12 months with high survival rate (up to 100%). The regenerants upon transfer to soil showed no morphological variation as compared with the donor plants. The medium optimized in the present study was applied for culture establishment and conservation of a total of 15 Brahmi accessions procured from different regions.     

In vitro conservation of Bacopa monnieri (L.) using mineral oil

Bacopa monnieri (L.) Wettst., a traditional Indian medicinal plant with high commercial potential, is used as a potent nervine tonic. A slow growth protocol was developed for medium-term conservation using mineral oil (MO) overlay. Nodal segments of B. monnieri (two genotypes; IC249250, IC468878) were conserved using MO for 24?months. Single node explants were implanted on MS medium supplemented with 0.2?mg?l^?1 BA and were covered with MO. Subculture duration could be significantly enhanced from 6 to 24?months, on the above medium. Normal plants regenerated from conserved cultures were successfully established in soil. On the basis of 20 random amplified polymorphic DNA and 5 inter-simple sequence repeat primers analyses and bacoside A content using HPLC, no significant reproducible variation was observed between the controls and in vitro-conserved plants. The results demonstrate the feasibility of using MO for medium-term conservation of B. monnieri germplasm without any adverse genetical and biochemical effects.     

Bacoside production by suspension cultures of Bacopa monnieri (L.) Pennell

Cell suspension cultures of Bacopa monnieri (L.) Pennell, grown in modified MS medium, grew some 5–6 fold over 40 days. Selected cell lines produced the important saponin, bacoside A, up to 1 g/100 g dry wt after this time.     

Correction to: Influence of explant types,non-embryogenic synseed and reduced oxygen environment on in vitro conservation of Bacopa monnieri (L.) Wettst

In Vitro Cellular & Developmental Biology - Plant - Readers should note the following error in the original version of this article: