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1.
Germinal cells in the goldfish retina that produce rod photoreceptors   总被引:1,自引:0,他引:1  
Dividing cells and their progeny in retinae of young goldfish were labeled with [3H]thymidine, and selected cells were reconstructed from serial sections processed for electron microscopic autoradiography. Our goals were to characterize the cells that were identified as rod precursors in previous light microscopic autoradiographical studies and to determine their origin and fate. (In fish the population of rods increases several-fold postembryonically by proliferation of rod precursor cells scattered across the retina). Over 200 labeled cells taken from 11 retinas were examined, and 20 of these were reconstructed in their entirety. Some retinas were examined at short intervals (1 to 48 hr) after [3H]thymidine injection in order to study mitotically active cells, and others were examined after longer intervals (9 or 14 days) to discover the nature of the progeny of labeled dividing cells. Previous evidence from thymidine studies in larval goldfish suggested that proliferating cells destined to produce rods appear first in the inner nuclear layer and later in the outer nuclear layer, where they continue to divide and generate new rods (P.R. Johns, (1982) J. Neurosci. 2, 179). The present results provide morphological evidence in support of the suggestion that rod precursors migrate from inner to outer nuclear layer and, furthermore, show that the precursors are closely associated with, and perhaps guided by, the radial processes of Müller glial cells. Examination of EM autoradiographs of labeled cells at 9 and 14 days after a pulse label with thymidine confirms that the differentiated progeny of dividing precursor cells are exclusively rods. To our knowledge, rod precursors are the first example of a neuronal germinal cell in the vertebrate central nervous system that under normal conditions produces only one type of neuron.  相似文献   

2.
The population of periodontal ligament (PDL) fibroblasts examined in this study may include osteogenic progenitor cells. PDL fibroblast and osteoblast kinetics in the periodontal ligament of the rat were measured following orthodontic stimulation of bone formation. Both single and multiple injections of tritiated thymidine (3H-TdR) were used. In single injection experiments, the peak percentage of PDL fibroblasts labeled with 3H-TdR is 15% at 22 hr post-stimulation. In multiple injection experiments, the total percentage of fibroblasts in the PDL which respond by synthesizing DNA is 50%. 3H-TdR-Iabeled osteoblasts appear at the same rate as, but with a time delay after, the labeled fibroblasts. Following stimulation, the most likely source of osteoblasts at the bone-forming site is not only fibroblasts which make DNA, divide, then differentiate, but also fibroblasts which either are differentiated to osteoblasts without DNA synthesis and cell division, or are released from G2 block by the orthodontic stimulation.  相似文献   

3.
The relationship of the acquisition of defining characteristics by precursor cells of sympathetic ganglia to the withdrawal of these cells from the cell cycle was investigated in the developing chick. The characteristics studied included the ability to synthesize catecholamines (CA), the development of characteristic subcellular storage granules, and the specific uptake of norepinephrine (NE). All were present in presumptive sympathicoblasts and adrenal medullary precursors, which also became labeled after the injection of tritiated thymidine and so retained the ability to divide. These dividing CA-containing cells were found in both primary ganglia and secondary preand paravertebral ganglia. The developing sympathetic neuronal population was found to be a heterogeneous one. Some sympathetic precursor cells appeared to become postmitotic (or to enter a pause in division) early in ontogeny, while others continued to divide throughout the time of hatching. As embryogenesis proceeded, the proportion of CA-containing cells or their precursors which were dividing decreased. However, those cells which did divide probably divided repeatedly. It is concluded that some of the definitive characteristics of mature neurons are expressed by dividing precursor cells. The specific characteristic that marks the transition from immature dividing cells to mature postmitotic neurons has not yet been determined.  相似文献   

4.
Treatments with tritiated thymidine (TdR-3H) have revealed the existence of two populations of mitotically active cells in meristems of lateral roots of Vicia faba. A rapidly dividing population, with a cycle time of 14 hr, constitutes about half the cells in the meristem. A second population of cells, with a cycle time in excess of 30 hr, is also present. Estimates of the relative size of this slowly dividing population are more difficult to make, but we calculate that this population includes 27–43% of meristem cells. The remaining fraction of the meristem is made up of cells that divide rarely or not at all. Since, at all times, both populations contribute to the mitotic index, the curve of the percentage of labeled mitoses that can be determined after a pulse label with TdR-3H differs from the curve expected of an ideal population in an important way: the peak value of the curve of the percentage of labeled mitoses is always less than 100%, usually between 75 and 80%. This heterogeneity within a meristem must be borne in mind in terms of the response of meristems to disruptive treatments, the mechanisms controlling mitotic cycle duration, and the spatial organization of a heterogeneous population in an organ that shows polarized growth.  相似文献   

5.
Summary Thymic nurse cell complexes (TNC-c), isolated from mouse thymuses at 1 and 2 h after i.v. injection of 6-(3H)thymidine, were analyzed in autoradiographs of semithin serial sections with regard to their size and the distribution of labeled thymocytes in individual types of complexes. The total number of thymocytes per complex reflects the type of complex. In a parallel study, localization of labeled thymocytes within individual zones of thymic cortex was examined. Thymocyte division within complexes may yield sequential complex generations differing in number per complex. However, thymocytes within complexes differ from each other in division kinetics. Half of the thymocytes that had been labeled 1 h after injection divided within 2 h. The rapidly dividing fraction of thymocytes were distributed within small complexes containing 2–8 cells and corresponded to the distribution of labeled cells in the outer thymic cortex. The proportion of labeled cells within large complexes resembled the distribution of labeled cells in the deep cortex. The data support the view that microenvironmental factors within TNC-c are responsible for both inducing thymocytes to enter the cell cycle and the negative selection (cell death) of some thymocytes.  相似文献   

6.
The mode of chromosome segregation in an asymmetrically dividing bacterium, Caulobacter crescentus, was studied by examining the fate of labeled DNA strands. Swarmer cells (one type of Caulobacter daughter cell), in which single strands of DNA had been labeled with [3H]thymidine during the previous round of chromosome replication, were grown synchronously in a non-radioactive medium for two generations. The distribution of radioactivity among the cells was visualized by autoradiography under a phase-contrast microscope. The labeled DNA strands in each cell were found to consist of two conserved units. From this, we propose a model in which the swarmer cell has two identical chromosomes, which are segregated into the progeny swarmer cell and the progeny stalked cell after chromosome replication.  相似文献   

7.
M.J. Masse  Isaac Harary   《Biochimie》1975,56(11-12)
BrdU and irradiation with visible light eliminates dividing cells from rat heart cell cultures. The lethal effect of light on the cultures is dependent upon the prior integration of BrdU into the DNA. Elimination of dividing cells is shown by the decreased uptake of 3H TdR in the remaining cells. Cultures in which the dividing cells were eliminated displayed a loss in myosin ATPase activity and a decrease in the rate of myosin ATPase activity increase, normally seen in control cultures. These results are consistent with the existence of cells at three stages of development; premyoblasts, which divide and contain no myosin; myoblasts which divide and contain myosin; and myocytes, which cannot divide and contain myosin. The results also indicate that the increase in myosin ATPase activity normally seen in heart cell cultures is a result of an increase in myosin in fully developed cells and in the increase in the number of cells capable of synthesizing myosin.  相似文献   

8.
In zebra finches only males sing, and several song control nuclei contain more neurons in adult males than in females. In the robust nucleus of the archistriatum (RA), this sex difference in neuron number arises because neuron survival is greater in young males than in females. The events initiating this sex difference in neuron survival are not known, but in earlier studies we observed that during sexual differentiation the proliferation and/or survival of RA cells exhibiting glial morphology is greater in males than in females. Because glia and glia-derived molecules are known to exert trophic effects on developing neurons, we wanted to determine when the sex difference in RA glia develops relative to the sexually dimorphic growth and survival of RA neurons. Male and female zebra finches were injected twice daily with 3[H]thymidine for 2 days beginning either on day 15 or 27. Two days later (day 18 or 30) sections through the RA were processed for autoradiography. Virtually all of the 3[H]thymidine labeled cells within the RA exhibited morphological features characteristic of glia and were not immunoreactive for the neuron-specific antigen, Hu. The number of these 3[H]thymidine labeled cells was measured, as were the number and soma size of RA neurons. Sex differences in RA neuron number and soma size were not evident at day 18, but emerged by day 30. However, at both ages the density of 3[H]thymidine labeled RA cells and their total number/RA neuron were significantly greater in males than in females. No such sexual dimorphism in the density of 3[H]thymidine labeled cells was evident in the archistriatum lateral to the RA, or within the RA of adult birds. These data indicate that sexually dimorphic gliogenesis is an early event in the sexual differentiation of the RA, preceding sex differences in RA neuron growth and survival. The possibility that glia (or glia-derived substances) may contribute to the neurotrophic effects of masculinization within the RA is discussed. © 1996 John Wiley & Sons, Inc.  相似文献   

9.
The number of dividing and DNA-synthesizing cells in excised pea roots can be regulated by eliminating the carbohydrate normally supplied in the culture medium. When the excised roots were allowed to remain for 24 hr in a medium lacking carbohydrate, the number of mitotic figures and tritiated thymidine (H3-T) labeled cells was reduced almost to zero. After an additional 24 hr in the incomplete culture medium, 15% of the interphase cells were H3-T labeled, the percentage of the cells that were dividing never exceeded 1.4, and 30% of these were H3-T labeled. When the roots remained in the deficient medium for 72 hr, neither cell division nor cells synthesizing DNA were observed. Upon addition of 2% sucrose, cell division and DNA synthesis were resumed in the roots that were maintained for 24 or 72 hr without an exogenous carbohydrate supply. It has been hypothesized that some proliferative systems consist of two cellular subpopulations which selectively stop or remain in either the pre-DNA synthetic (G1) or post-DNA synthetic (G2) periods of the mitotic cycle. The addition of sucrose, H3-T, and 5-aminouracil to the medium, after the roots had been maintained for 24 hr without a carbohydrate, indicated that most of the proliferative cells in the roots had accumulated in either G1, a quasi-G1 condition, i.e., DNA synthesis stopped sometime before completion, or G2 periods of interphase; the majority, however, were in G1 or quasi-G1 conditions. The results suggested that DNA synthesis (S period) and mitosis or the onset of these processes have the highest metabolic requirements in the mitotic cycle and that G1 and G2 were the most probable states for proliferative cells in a meristem with a low metabolic level.  相似文献   

10.
Summary The secretory tissue of the uropygial gland is of the holocrine type, containing both dividing progenitor cells and lipid-filled differentiated cells. In this study, we examined the relationship between cell division and differentiation. The location of dividing cells was determined by autoradiography of tissue sections from ducklings injected intra-abdominally with 3H-thymidine. Only cells on the basal lamina of the tubules contained labeled nuclei. Dividing cells were distributed uniformly over the length of the tubules. Over the next five days, most of the labeled cells migrated to the lumen of the tubules and disappeared. Cells containing the lipogenic enzymes, fatty acid synthase and malic enzyme, were localized either immunocytochemically using affinity-purified antibodies or cytochemically using a specific assay for malic enzyme activity. Fatty acid synthase and malic enzyme were undetectable in dividing basal cells but present at high levels in differentiating and differentiated cells. Thus, basal cells lying along the basal lamina of the tubules were replacing lipid-laden cells that were continually sloughed into the lumens of the tubules. The signals for differentiation and enzyme accumulation appear to be linked to one another and to cessation of cell division.  相似文献   

11.
Measurements of bacterial secondary production were carried out during 13 diel studies at one coastal marine station and in five lakes differing with respect to nutrient concentration and primary production. Bacterial secondary production was measured in situ every 3 to 5 h by [3H]thymidine incorporation into DNA. In some of the diel studies, these results were compared with results obtained from dark 14CO2 uptake and frequency of dividing cells. Only minor diel changes were observed. The rate of [3H]thymidine incorporation into DNA and the frequency of dividing cells varied from 23 to 194% of the diel mean. The dark CO2 uptake rate varied from 12 to 259% of the diel mean. An analysis of variance demonstrated that no specific time periods during 24 h showed significantly different production rates, supporting the idea that bacterial activities in natural assemblages are controlled by a variety of events. The best correction (r2 = 0.74) was obtained between the [3H]thymidine incorporation and frequency of dividing cells procedures from the lake water samples. The actual production rates calculated by [3H]thymidine incorporation into DNA were appreciably lower than those obtained by the frequency of dividing cells and the dark CO2 uptake techniques. Diel rates of bacterial production are discussed in relation to sampling frequency, statistical errors, and choice of method.  相似文献   

12.
Irmeli Palmberg 《Protoplasma》1990,158(3):109-120
Summary A combination of microscopical, immunocytochemical, and autoradiographic techniques were employed to study stem cells and their fates during asexual reproduction and regeneration in two microturbellarians,Microstomum lineare (Macrostomida) andStenostomum leucops (Catenulida). Special attention was paid to the development of the immunoreactivity (IR) to FMRF/RF-amide and 5-HT in differentiating nerve cells.Asexual reproduction inM. lineare andS. leucops occurs by paratomy, i.e., fragmentation after completed differentiation of the new organs. Regeneration, on the other hand, involves a combination of morphallactic and epimorphic processes without the formation of a regeneration blastema. The only cells incorporating tritiated thymidine ([3H]T) were the mesenchymal and gastrodermal neoblasts, which proliferate continuously replenishing the population of stem cells available for growth, asexual reproduction and regeneration. These proliferative cells occurred in two ultrastructurally different forms, differing from each other only by the presence or absence of ciliar basal bodies in the cytoplasm. Few differentiated cells were labeled in the head piece after completed regeneration. A greater amount of labeled differentiated cells were, however, observed postpharyngeally in the first zooid as well as in zooids having developed during the same time (i.e., 20–45 h after the treatment with [3H]T). Furthermore, many labeled cells were still undifferentiated at that time or just in the beginning of the differentiation process. It can therefore be concluded that neoblasts function both as reserve cells and as functional stem cells for all differentiated cell types in these worms. IR to FMRF/RF-amide neuropeptides was not observed in nerve cells differentiating from neoblasts until the occurrence of dense-core vesicles in their cytoplasm. Due to methodological difficulties only weak or no IR to 5-HT could be traced in the nervous system of the asexual and regenerating worms.Abbreviations ICC Immunocytochemical - IR immunoreactivity - [3H]T tritiated thymidine  相似文献   

13.
Schwann cell proliferation induced by a myelin-enriched fraction was examined in vitro. Although nearly all the Schwann cells contained material that was recognized by antisera to myelin basic protein after 24 h, only 1% of the cells were synthesizing DNA. 72 h after the addition of the mitogen a maximum of 10% of the cells incorporated [3H]thymidine. If the cultures were treated with the myelin-enriched fraction for 24 h and then washed, the number of proliferating Schwann cells decreased by 75% when compared with those cells that were incubated with the mitogen continuously. When Schwann cells were labeled with [14C]thymidine followed by a pulse of [3H]thymidine 24 h later, every Schwann cell labeled with [3H]thymidine was also labeled with [14C]thymidine. Although almost every Schwann cell can metabolize the myelin membranes within 24 h of exposure, a small population of cell initially utilizes the myelin as a mitogen, and this population continues to divide only if myelin is present in the extracellular media. The percentage of the Schwann cells that initially recognize the myelin-enriched fraction as a mitogen is dependent upon the age of the animal from which the cells were prepared.  相似文献   

14.
Roots of Vicia faba were treated with H3-thymidine (1 c/ml) for 1 h and incorporation of H3-TdR into nuclei of primordia was studied. Large primordia (>1,500 cells) did not incorporate H3-TdR. Cells of small primordia did not incorporate the labeled precursor immediately but did so after a delay of several hours. The frequency of labeled nuclei became similar to that of lateral meristems only after a delay of 12–14 h. The gradual increase in labeling index also occurs in colchicine treated cells, which do not divide; this shows that the rise in labeling index is not due solely to the division of labeled cells. It has been estimated from H3-TdR and colchicine labeled cells that small primordia have a population of cells in which intermitotic time is about 12 h. The delay in incorporation appears to indicate that pools of H3-TdR can be maintained in small primordia for several hrs and that the precursor is not used until the cells synthesize thymidine kinase.This research has been supported by the U.S.A.E.C. [Grant AT (11-1) 1625-12].  相似文献   

15.
Abstract Advances in molecular techniques have enabled new approaches to identifying bacteria. However, once identified, there is no quantitative information on the in situ growth rate of the species, mainly because the technology has not been available. The quantitative incorporation of [methyl-3H]thymidine into dividing bacteria is coupled with a molecular (hybridization) method, to determine the growth rate of bacterial species in situ. The basis of this molecular method is a reverse gene probe—natural populations are labeled in situ with [methyl-3H]thymidine. The probe (3H-Tdr-DNA) is captured, using a hybridization procedure, on a positively charged nylon membrane on which is attached non-labeled target DNA. Two bacterial species, Bacillus cereus and Zoogloea ramigera, were used to demonstrate the principle in laboratory cultures and in a municipal activate sludge treatment process. The DNA of the dividing bacteria in activated sludge was radioactively labeled with [methyl-3H]thymidine, and the DNA of Z. ramigera was recovered using a DNA hybridization method. The recovered radioactively-labeled DNA was used to estimate the growth rate (0.03 × 109 cells · ml−1· h−1) of Z. ramigera in situ. Simultaneously applying these two powerful molecular-based methods could potentially be used to study bacterial population dynamics in situ. Received: 10 September 1997; Accepted: 5 January 1998  相似文献   

16.
Summary The pseudostratified tracheal epithelium, composed of a heterogeneous phenotypically varying cell population, was studied with respect to the in vitro cell proliferative activity of differentiated epithelial cells. Ciliated tracheal epithelial cells so far have been considered to be terminally differentiated, nonproliferating cells. Tracheal organ cultures obtained from vitamin A-deprived Syrian Golden hamsters were cultured in a vitamin A-deficient, serum-free, hormone-supplemented medium. In vitamin A-deprived tracheal epithelium treated with physiologically active all-trans retinol and low cigarette-smoke condensate concentrations it is possible to stimulate the cell proliferation of both basal and columnar cells. Therefore, the probability of finding proliferating columnar cells was increased compared with the in vivo and the vitamin A-deprived situation in which cell proliferative activity is relatively low. In the presence of cigarette-smoke condensate in a noncytotoxic concentration, basal, small mucous granule, ciliated, and indifferent tracheal epithelial cells incorporated [methyl-3H]-thymidine into the DNA during the S phase. The finding that ciliated cells were labeled was supported by serial sections showing the same labeled ciliated cell in two section planes separated by 2 to 3 μm, without labeled epithelial cells next to the ciliated cell. Furthermore, a ciliated tracheal epithelial cell incorporating [methyl-3H]thymidine into DNA was also seen in tracheal cultures of vitamin A-deprived hamsters treated with all-trans retinol in a physiologic concentration. The present study was financially supported by the Scientific Advisory Committee on Smoking and Health (Dutch Cigarette Industry Foundation) and the Ministry of Welfare, Health and Cutural Affairs.  相似文献   

17.
Pieces of mouse embryonic pancreatic epithelium cultured in an inductive situation in vitro, or when examined at critical times in vivo, show a gradient of zymogen granule accumulation. Cells located internally in explants, or in central acini in vivo, show this overt differentiation first. As the epithelia age, the more peripheral cell population proceeds in a similar differentiation. Observations of autoradiograms of H3-thymidine-labeled tissues indicate that the first cells which cease incorporating the DNA-precursor are in the central regions that differentiate first. In older explants, thymidine incorporation is largely restricted to the periphery of the tissue as zymogen appears in the internal cells. Evidence suggests that cells or nuclei which have replicated DNA move inward before dividing. Some daughter cells apparently return peripherad to divide again, whereas others remain centrally where they undergo differentiation. During at least the first 24 hours of these maturational changes, mesenchyme has a stimulatory effect upon epithelial thymidine-incorporation frequencies. The presence of a post-DNA-synthetic population is seen in the form of a group of nonlabeling central cells that remains intact in the midst of a labeled epithelium for as long as 48 hours in vitro (from 72 to 120 hours). If explants are treated with 5-bromodeoxyuridine for any 24-hour segment of the 0 to 72-hour period, before the non-incorporating population arises, no subsequent overt zymogen formation occurs. If explants are treated continuously from 72 to 120 hours, on the other hand, zymogen still forms in some internal cells. Presumably, this differentiation is limited to the postmitotic population as revealed in the thymidine autoradiograms.  相似文献   

18.
RENEWAL OF TASTE BUD CELLS IN RAT CIRCUMVALLATE PAPILLAE   总被引:12,自引:0,他引:12  
The life span of taste bud cells in rat circumvallate papillae was measured by autoradiography after labeling them with a pulse of [3H]thymidine. Specimens of circumvallate papillae were taken daily 1·5-18·5 days after the isotope was administered; thereafter, specimens were taken on alternate days until 25·5 days. For each time interval, the number of labeled cell nuclei was counted in 200-450 taste buds and plotted as the ratio of labeled cells/taste bud v. time after injection of [3H]TdR. In all, 6958 taste buds were counted. The total number of labeled cells (dark plus light) per taste bud reached peaks at 6·5, 13·5 and 20·5 days. The curve for the number of labeled dark cells/bud had essentially the same shape as that for total cells. The number of labeled light cells/bud reached a modest peak at 6·5 days and slowly declined to a plateau for the remainder of the experiment. The data show that an average of 2 days elapsed after injection before labeled dark cells entered the bud and they spent an average of 7 days in the non-proliferating taste bud compartment; thus, the life span of the dark cell was 9 days. The life span of the light cell was difficult to estimate quantitatively, but this cell type was labeled at a much slower rate than dark cells and is assumed to have a significantly longer tenure in the taste bud.  相似文献   

19.
The purpose of this study was to determine the depth distribution of bacterial biomass and production in a stratified lake and to test techniques to measure bacterial production in anaerobic waters. Bacterial abundance and incorporation of both [3H]thymidine and [3H]leucine into protein were highest in the metalimnion, at the depth at which oxygen first became unmeasurable. In contrast, [3H]thymidine incorporation into DNA was highest in the epilimnion. The ratios of incorporation into DNA/protein averaged 2.2, 0.49, and 0.95 for the epilimnion, metalimnion, and hypolimnion, respectively. Low incorporation into DNA was not due to artifacts associated with the DNA isolation procedure. Recovery of added [3H]DNA was about 90% in waters in which the portion of [3H]thymidine incorporation into DNA was about 40%. At least some obligate anaerobic bacteria were capable of assimilating thymidine since aeration of anaerobic hypolimnion waters substantially inhibited thymidine incorporation. The depth profile of bacterial production estimated from total thymidine and leucine incorporation and the frequency of dividing cells were all similar, with maximal rates in the metalimnion. However, estimates of bacterial production based on frequency of dividing cells and leucine incorporation were usually significantly higher than estimates based on thymidine incorporation (using conversion factors from the literature), especially in anaerobic hypolimnion waters. These data indicate that the thymidine approach must be examined carefully if it is to be applied to aquatic systems with low oxygen concentrations. Our results also indicate that the interface between the aerobic epilimnion and anaerobic hypolimnion is the site of intense bacterial mineralization and biomass production which deserves further study.  相似文献   

20.
The cellular mechanisms that underlie formation of an autonomic ganglion have been investigated by studying the formation of the cardiac ganglion of the frog. Analysis of the genesis of neurons with [3H]thymidine autoradiography revealed that neuronal precursors do not divide via a “stem cell lineage” but rather divide exponentially, such that both daughter cells either re-enter the mitotic cycle or differentiate. Neurogenesis in this autonomic ganglion is prolonged, beginning during the second day after fertilization and continuing for at least 2 weeks. The use of acetylcholinesterase (AChE) as a neuronal marker showed that differentiated neurons start condensing in their target 1.5 days after the first neurons are born. Neurons accumulate, concomitant with neurogenesis, at a constant rate of approximately six neurons per day. Transplantation and organ culture demonstrated that immature neurons are present well before definitive expression of the mature phenotype and that their initial expression does not depend upon maintained contact by preganglionic axons.  相似文献   

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