共查询到20条相似文献,搜索用时 93 毫秒
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Nucleolar organization of HeLa cells as studied by in situ hybridization 总被引:17,自引:0,他引:17
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High resolution detection of rRNA and rDNA in plant nucleoli with different activities by in situ hybridization 总被引:2,自引:0,他引:2
Bassy O Jiménez-García LF Echeverría OM Vázquez-Nin GH Díaz de la Espina SM 《Biology of the cell / under the auspices of the European Cell Biology Organization》2000,92(1):59-70
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Nucleolonema as a fundamental substructure of the nucleolus 总被引:1,自引:0,他引:1
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In situ hybridization at the electron microscope level: an improved method for precise localization of ribosomal DNA and RNA 总被引:8,自引:0,他引:8
In situ hybridization using biotinylated rDNA probes and secondary antibody coupled to gold particles was developed on ultrathin sections of Lowicryl-embedded Ehrlich tumor cells for precise localization of ribosomal RNA (rRNA) and ribosomal DNA (rDNA). For the detection of rDNA, an immunocytochemical approach involving an antibody against single-stranded DNA was used in order to determine the more efficient denaturation procedure. Using this technique, rDNA can be visualized in the fibrillar centers of nucleoli, especially in their peripheral regions at the proximity of both the dense fibrils and the nucleolar interstices as well as within the latter. rDNA was occasionally detected in some clumps of dense nucleolus-associated chromatin. Besides the presence of rRNA in the ribosome-rich cytoplasmic areas and in the dense fibrillar component and the granular component of the nucleolus, rRNA was also found in the fibrillar center areas close to the boundary region to the dense fibrillar component. These results are discussed in the light of the present knowledge on the functional organization of the nucleolus. 相似文献
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Npa1p, a component of very early pre-60S ribosomal particles, associates with a subset of small nucleolar RNPs required for peptidyl transferase center modification 下载免费PDF全文
Dez C Froment C Noaillac-Depeyre J Monsarrat B Caizergues-Ferrer M Henry Y 《Molecular and cellular biology》2004,24(14):6324-6337
We have identified a novel essential nucleolar factor required for the synthesis of 5.8S and 25S rRNAs termed Npa1p. In the absence of Npa1p, the pre-rRNA processing pathway leading to 5.8S and 25S rRNA production is perturbed such that the C2 cleavage within internal transcribed spacer 2 occurs prematurely. Npa1p accumulates in the immediate vicinity of the dense fibrillar component of the nucleolus and is predominantly associated with the 27SA2 pre-rRNA, the RNA component of the earliest pre-60S ribosomal particles. By mass spectrometry, we have identified the protein partners of Npa1p, which include eight putative helicases as well as the novel Npa2p factor. Strikingly, we also show that Npa1p can associate with a subset of H/ACA and C/D small nucleolar RNPs (snoRNPs) involved in the chemical modification of residues in the vicinity of the peptidyl transferase center. Our results suggest that 27SA2-containing pre-60S ribosomal particles are located at the interface between the dense fibrillar and the granular components of the nucleolus and that these particles can contain a subset of snoRNPs. 相似文献
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