共查询到20条相似文献,搜索用时 15 毫秒
1.
Thomas Bartolomaeus 《Zoomorphology》1989,109(1):15-32
Different developmental stages (trochophores, nectochaetae, non-mature and mature adults) of Anaitides mucosa were investigated ultrastructurally. A. mucosa has protonephridia throughout its life; during maturity a ciliated funnel is attached to these organs. The protonephridial duct cells are multiciliated, while the terminal cells are monociliated. The single cilium is surrounded by 14 microvilli which extend into the duct lumen without coming into any contact with the duct cells. Corresponding ultrastructure and development indicate that larval and adult protonephridia are identical in A. mucosa. Differences between various developmental stages can be observed only in the number of cells per protonephridium. A comparison between the funnel cells, the cells of the coelothel and the duct cells reveals that the ciliated funnel is a derivative of the duct. Due to the identical nature of the larval and postlarval protonephridia, such a funnel cannot be a secondary structure. In comparison with the mesodermally derived metanephridial funnel in phoronids it seems likely that the metanephridia of annelids and phoronids evolved convergently. 相似文献
2.
In the initial part of the protonephridium of E. longa specialized cells, cyrtocytes, are observed in addition to cells of the protonephridial duct among which dark and light cells are distinguished. The metanephridial duct of L. terrestirs is characterised by a comples differentiation and subdivision into several parts whose cells deffer in their morphology. The functional role of the two excretory organs is discussed. 相似文献
3.
A new hesionid. Sinohesione genitaliphora gen. et sp. n., is described from intertidal sandy sediments of Hainan Island, China. It differs from hitherto known hesionids by the presence of external genital organs in both sexes. In the males there is one pair of sae-like appendages, each bearing a tube-shaped penis, on chaetiger 10. In the females the paired sae-shaped organs are situated on chaetiger 12. Reconstructions of semi- and ultrathin sections show that a long, heavily coiled sperm duct opens at the tip of each penis. The duct opens with a ciliated funnel into a seminal vesicle in chaetiger 9. Prominent gland cells surround the sperm duct for the most part. The female genital organs each have two openings; one of which leads to a blind ending seminal receptacle. The other is the external pore of a ciliated oviduct that originates as an open funnel in the coelom of chaetiger 10. The functional and phylogenetic significance of these structures is discussed. 相似文献
4.
Mullinger AM 《Tissue & cell》1969,1(1):31-52
Three types (A, B, and C) of ampullary sense organs occur in the skin of Gymnarchus niloticus. In type A the ampulla is connected to the surface of the skin by an open duct whereas in B and C organs it is closed, though overlain by specialized epidermal cells. In each case the receptor cell surface in contact with the ampullary lumen bears microvilli; these are more highly developed in B and C organs than in type A. Fine structural observations are consistent with the view that the organs are electric receptors of three different types. 相似文献
5.
The growth of a tumour in a duct is examined in order to model ductal carcinoma in situ (DCIS) of the breast, the earliest known stage of breast cancer. Interactions between the expansive forces created by tumour cell proliferation and the stresses that develop in the compliant basement membrane are studied using numerical and analytical techniques. Particular attention focuses on the impact that proteolytic enzymes have on the tumour's progression. As the tumour expands and the duct wall deforms, the tumour cells are subjected to mechanical and nutritional stresses caused by high pressures and oxygen deprivation. Such stresses may stimulate the cells to produce proteolytic enzymes that degrade the duct wall, making it more compliant and prone to penetration by the tumour cells. We use our model to compare these two hypotheses for enzyme production and find that mechanical stress is likely the dominant mechanism, with the wall deforming most at the centre of the duct. We then discuss the biological implications of our theoretical results and suggest possible directions for future work. 相似文献
6.
D F Eberl 《Current opinion in neurobiology》1999,9(4):389-393
To hear, insects use diverse external structures, which transform acoustic signals to mechanical ones, coupled to astonishingly uniform mechanosensory transducers, the chordotonal organs. New evidence showing that chordotonal organs and vertebrate auditory hair cells are developmentally related and that chordotonal organs and insect bristle organs are mechanistically related suggests that all these ciliated mechanoreceptors may be derived from the same ancestral molecular mechanotransduction complex. Identification of these elusive molecules will settle this issue. 相似文献
7.
Preferential localization of calcium-activated neutral protease in epithelial tissues 总被引:1,自引:0,他引:1
M Hayashi Y Kasai S Kawashima 《Biochemical and biophysical research communications》1987,148(2):567-574
Immunohistochemical localization of calcium-activated neutral protease (CANP) in rabbit organs was determined using a monoclonal antibody against CANP. In most organs, epithelial tissues reacted intensely: these tissues include great alveolar and squamous alveolar cells in lung; interlobular artery, vein, and bile duct in liver; small vessels in skeletal muscle; glomeruli, juxtanglomerular cells, distal and collecting tubules in kidney; mucous epithelium in gallbladder; interstitial cells in testis; and cuboidal epithelial cells in brain choroid plexus. On the other hand, hepatocytes, epithelial cells which have ill defined basal lamina, were stained very faintly. These observations suggest that the physiological function of CANP is involved with transport systems in epithelial tissues through basal lamina. 相似文献
8.
《Biophysical journal》2021,120(19):4142-4148
The inner ear is one of the most complex structures in the mammalian body. Embedded within it are the hearing and balance sensory organs that contain arrays of hair cells that serve as sensors of sound and acceleration. Within the sensory organs, these hair cells are prototypically arranged in regular mosaic patterns. The development of such complex, yet precise, patterns require the coordination of differentiation, growth, and morphogenesis, both at the tissue and cellular scales. In recent years, there is accumulating evidence that mechanical forces at the tissue, the cellular, and the subcellular scales coordinate the development and organization of this remarkable organ. Here, we review recent works that reveal how such mechanical forces shape the inner ear, control its size, and establish regular cellular patterns. The insights learned from studying how mechanical forces drive the inner ear development are relevant for many other developmental systems in which precise cellular patterns are essential for their function. 相似文献
9.
The ontogenesis and structural characteristics of the seminal vesicles in Clarias gariepinus (sharptooth catfish) were studied by light and electron microscopy and are described in detail. The seminal vesicles, beginning as simple protrusions from the vas efferentia, becomes more complex with age. Their distal ends become fingerlike and the bases form palm-like extensions. Juvenile male organs do not reveal any signs of seminal vesicles although spermatogenic tissue is already well delineated. The developing gonads contain clusters of large cells, close to the sperm duct and cysts of the testis, from which seminal vesicles are formed. Secretory epithelium lines the tubules of the seminal vesicles and becomes columnar as the tissue matures. Electron micro-graphs of these epithelial cells reveal two types of cells: opaque cells and cells with very vacuolized cytoplasm. Dense pinocytotic vesicles are present between the membranes of neighbouring seminal tubules and apical cell membranes facing the lumen. Maturation and onset of secretion by the secretory cells is accompanied by morphological changes. Protruding cylindrical cells become shortened, modified to cuboidal, rounded cells that send tubular extensions into the lumen. In the final stage of differentiation, only connective tissue membranes supporting the tubule walls remain intact. At the points of contact between the testis, seminal vesicles, and sperm duct, the epithelia of these organs often become confluent. The distal parts of the seminal vesicles, rarely contain sperm; during spawning sperm accumulated in the proximal tubules of the vesicles. © 1994 Wiley-Liss, Inc. 相似文献
10.
W H Arnold 《The Histochemical journal》1984,16(7):755-769
Summary Various organs of three species of monkey were screened histochemically for esteroproteases usingN-acethyl-l-methionine--naphthylester ( N-O-met) as the substrate and also for enzymes with kallikrein-like activity usingd-Val-Leu-Arg-4-methoxy-2-naphthylamide as the substrate. Characteristic differences were found in the localization of the reaction products obtained with both substrates. In the main salivary glands, esteroproteases ( N-O-met reactivity) were found in mucous cells (submandibular gland), intercalated duct cells (parotid gland), acinar cells (sublingual gland), striated and interlobular duct cells (all glands). They were also localized in superficial lining epithelial cells of the digestive system, in liver cells, and acinar cells of the pancreas.Enzymes with kallikrein-like activity were found only in the striated and interlobular duct cells of salivary glands, in acinar cells of the pancreas, and in proximal tubular cells of the kidney. Free cells (including mast cells) normally distributed in the connective tissue of various organs showed reactivity towards N-O-met. Some of these cells were also reactive against Val-Leu-Arg-4-MNA. 相似文献
11.
Differentiation is the process by which tissues/organs take on their final, physiologically functional form. This process is mediated in part by the silencing of embryonic genes and the activation of terminal, differentiation gene products. Mammalian kidney development is initiated when the Wolffian duct branches and invades the overlying metanephric mesenchyme. The newly formed epithelial bud, known as the ureteric bud, will continue to branch ultimately differentiating into the collecting duct system and ureter. Here, we show that Hoxb7-Cre mediated removal of β-catenin from the mouse Wolffian duct epithelium leads to the premature expression of gene products normally associated with the differentiated kidney collecting duct system including the water channel protein, Aquaporin-3 and the tight junction protein isoform, ZO-1α+. Mutant cells fail to maintain expression of some genes associated with embryonic development, including several mediators of branching morphogenesis, which subsequently leads to kidney aplasia or hypoplasia. Reciprocally, expression of a stabilized form of β-catenin appears to block differentiation of the collecting ducts. All of these defects occur in the absence of any effects on the adherens junctions. These data indicate a role for β-catenin in maintaining cells of the Wolffian ducts and the duct derived ureteric bud/collecting duct system in an undifferentiated or precursor state. 相似文献
12.
Orvis GD Jamin SP Kwan KM Mishina Y Kaartinen VM Huang S Roberts AB Umans L Huylebroeck D Zwijsen A Wang D Martin JF Behringer RR 《Biology of reproduction》2008,78(6):994-1001
Amniotes, regardless of genetic sex, develop two sets of genital ducts: the Wolffian and Müllerian ducts. For normal sexual development to occur, one duct must differentiate into its corresponding organs, and the other must regress. In mammals, the Wolffian duct differentiates into the male reproductive tract, mainly the vasa deferentia, epididymides, and seminal vesicles, whereas the Müllerian duct develops into the four components of the female reproductive tract, the oviducts, uterus, cervix, and upper third of the vagina. In males, the fetal Leydig cells produce testosterone, which stimulates the differentiation of the Wolffian duct, whereas the Sertoli cells of the fetal testes express anti-Müllerian hormone, which activates the regression of the Müllerian duct. Anti-Müllerian hormone is a member of the transforming growth factor-beta (TGF-beta) family of secreted signaling molecules and has been shown to signal through the BMP pathway. It binds to its type II receptor, anti-Müllerian hormone receptor 2 (AMHR2), in the Müllerian duct mesenchyme and through an unknown mechanism(s); the mesenchyme induces the regression of the Müllerian duct mesoepithelium. Using tissue-specific gene inactivation with an Amhr2-Cre allele, we have determined that two TGF-beta type I receptors (Acvr1 and Bmpr1a) and all three BMP receptor-Smads (Smad1, Smad5, and Smad8) function redundantly in transducing the anti-Müllerian hormone signal required for Müllerian duct regression. Loss of these genes in the Müllerian duct mesenchyme results in male infertility due to retention of Müllerian duct derivatives in an otherwise virilized male. 相似文献
13.
The human ear is capable of processing sound with a remarkable resolution over a wide range of intensity and frequency. This ability depends largely on the extraordinary feats of the hearing organ, the organ of Corti and its sensory hair cells. The organ of Corti consists of precisely patterned rows of sensory hair cells and supporting cells along the length of the snail-shaped cochlear duct. On the apical surface of each hair cell, several rows of actin-containing protrusions, known as stereocilia, form a "V"-shaped staircase. The vertices of all the "V"-shaped stereocilia point away from the center of the cochlea. The uniform orientation of stereocilia in the organ of Corti manifests a distinctive form of polarity known as planar cell polarity (PCP). Functionally, the direction of stereociliary bundle deflection controls the mechanical channels located in the stereocilia for auditory transduction. In addition, hair cells are tonotopically organized along the length of the cochlea. Thus, the uniform orientation of stereociliary bundles along the length of the cochlea is critical for effective mechanotransduction and for frequency selection. Here we summarize the morphological and molecular events that bestow the structural characteristics of the mammalian hearing organ, the growth of the snail-shaped cochlear duct and the establishment of PCP in the organ of Corti. The PCP of the sensory organs in the vestibule of the inner ear will also be described briefly. 相似文献
14.
Preferential expression of the c-fps protein in chicken macrophages and granulocytic cells. 总被引:13,自引:6,他引:7
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We have studied the expression of the protein kinase activity of NCP98, the c-fps gene product, in several hemopoietic tissues of chickens as a function of the developmental stage of these organs. We found that in bone marrow, spleen, and bursa, maximum NCP98 kinase activity on a per-cell basis correlates with the peak of granulopoiesis in these organs. Furthermore, in a bovine serum albumin density gradient fractionation of bone marrow cells, granulocytic cells appeared to account for most of the NCP98 kinase activity. No correlation was found between the distribution of erythrocytic, lymphocytic, or thrombocytic cells and the distribution of the expression of NCP98 kinase activity. However, NCP98 protein and kinase activity were 10-fold higher in macrophages than in bone marrow. In addition, depletion by complement-mediated lysis of erythrocytic cells in bone marrow did not significantly reduce the total recovery of NCP98 kinase activity. These results argue for the specific expression of the c-fps gene product in granulocytic cells and macrophages. 相似文献
15.
Mierke CT 《Cell biochemistry and biophysics》2011,61(2):217-236
The malignancy of tumors depends on the biomechanical properties of cancer cells and their microenvironment, which enable
cancer cells to migrate through the connective tissue, transmigrate through basement membranes and endothelial monolayers
and form metastases in targeted organs. The current focus of cancer research is still based on biological capabilities such
as molecular genetics and gene signaling, but these approaches ignore the mechanical nature of the invasion process of cancer
cells. This review will focus on how structural, biochemical and mechanical properties of extracellular matrices (ECMs), and
adjacent cells regulate the invasiveness of cancer cells. In addition, it presents how cancer cells create their own microenvironment
by restructuring of the ECM and by interaction with stromal cells, which then further contribute to the progression of cancer
disease. Finally, this review will point out that mechanical properties are a critical determinant for the efficiency of cancer
cell invasion and the progression of cancer which might affect the future development of new cancer treatments. 相似文献
16.
Scanning and transmission electron microscopy of the female reproductive system of Schistosoma margrebowiei Le Roux, 1933 总被引:1,自引:0,他引:1
Transmission electron microscopy shows that the uterus of female Schistosoma margrebowiei possesses the same ultrastructure as that of the tegument but lacks spines and sense organs. It does not possess secretory cells and opens at the gonopore which by scanning electron microscopy was seen to be composed of numerous leaf-like protrusions. The morphology of the ovary is comparable with that of other Digenea. Immature and mature ova possess cortically arranged granules and occur within the posterior zone of the ovary. Cilia and lamellae line the luminal surface of the oviduct and ootype, the lamellae running unidirectionally along the duct. Only a single type of secretory cell is seen within Mehlis' gland and this produces dense bodies which are associated with Goldi bodies. Narrow cytoplasmic channels supported by microtubules deliver these secretory bodies to the ootype. The vitelline duct is lined with cilia and lamellae and the vitelline gland contains four types of cells, S1, S2, S3 and S4. Calcareous corpuscles are found within mature S4 cells. 相似文献
17.
18.
Studies were carried out to define antigenic characteristics of the rabbit renal collecting duct. Renal papillae of adult rabbits were homogenized, centrifuged, and the 600 X g pellet was extracted with 0.5% Triton X-100 in the presence of 1 M NaCl. The crude extract was fractionated on an anion exchange column (DEAE cellulose). A fraction enriched in acidic proteins that co-purified with a radioactive 150 kd glycoprotein from cultured collecting duct cells (Minuth 1982), was used for immunization of guinea pigs. The antiserum shows the following characteristics as revealed by indirect immunofluorescence on the rabbit kidney: 1) Among all tubular epithelial cells only principal cells of the collecting duct and the connecting tubule cell show immunoreactivity. 2) The antiserum decorates the epithelial-interstitial interface of the whole collecting duct as well as of connecting tubule and thick ascending limb of Henle's loop. 3) There is immunoreactivity of interstitial fibers throughout the kidney. 4) Epithelial cells in a variety of other organs in rabbit did not react with the antiserum. Our data demonstrate an antigenic distinction of both, the connecting tubule cell and the principal cell, discriminating these cells from other tubular epithelial cells including the intercalated cells of the collecting duct system. Furthermore, our findings point to a heterogeneity along the distal nephron with respect to the constituents of the epithelial-interstitial interface. 相似文献
19.
Thomas Bartolomaeus 《Zoomorphology》1989,109(2):113-122
Summary The actinotrocha of Phoronis muelleri has one pair of ectodermally derived, monociliated protonephridia. The duct runs mainly between the epidermis and the lining of the hyposphere coelom, pierces the septum and extends into the blastocoel. The proximal part is branched and closed up by terminal complexes consisting of two morphologically different cells which both serve filtration. During metamorphosis, the terminal complexes and the branches of the duct are cast off. The cells degenerate, pass into the remaining duct and are endocytosed by the duct cells. After metamorphosis the remaining part of the protonephridial duct is U-shaped, blindly closed and borders on the prospective lophophoral vessel. In a later stage the duct receives a ciliated funnel, which consists of monociliated epithelio-muscle cells and is a derivative of the lining of the metacoel. Thus, a part of the protonephridial duct of the larva and the whole metanephridial duct of the adult are identical. Aspects of a possible homology between phoronid nephridia and such organs in other bilaterians are discussed. 相似文献
20.
José M. Icardo Elvira Colvee Eugenia R. Lauriano Gioele Capillo Maria C. Guerrera Giacomo Zaccone 《Journal of morphology》2015,276(1):90-101
We report here on the macroscopic, light microscopic, and electron microscopic structure of the gas bladder (GB) of the spotted gar, Lepisosteus oculatus. The GB opens into the pharynx, dorsal to the opening of the oesophagus, through a longitudinal slit bordered by two glottal ridges. Caudal to the ridges, the GB is an elongated sac divided into a central duct and right and left lobes. The lobes are formed by a cranio‐caudal sequence of large air spaces that open into the central duct. The structure of the GB is that of a membranous sac supported by a system of septa arising from the walls of a central duct. The septa contain variable amounts of striated and smooth muscle might function to maintain the bladder shape and in providing contractile capabilities. The presence of muscle cells, nerves, and neuroepithelial cells in the wall of the GB strongly suggests that GB function is tightly regulated. The central duct and the apical surface of the thickest septa are covered by mucociliated epithelium. Most of the rest of the inner bladder surface is covered by a respiratory epithelium which contains goblet cells and a single type of pneumocyte. These two cell types produce surfactant. The respiratory barrier contains thick areas with fibrillar material and cell prolongations, and thin areas that only contain basement membrane material between the capillary wall and the respiratory epithelium. Lungs and GBs share many anatomical and histological features. There appears to be no clear criterion for structural distinction between these two types of respiratory organs. J. Morphol. 276:90–101, 2015. © 2014 Wiley Periodicals, Inc. 相似文献