共查询到19条相似文献,搜索用时 103 毫秒
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不同活力玉米种子胚萌发期间热激蛋白的合成 总被引:8,自引:0,他引:8
玉米(Zea mays L.)种子在萌发期间热激处理(42℃)时蛋白质合成率低于对照(25℃);高活力种子胚热激蛋白的合成率最高。在42℃热激处理时玉米种胚子合成的热激蛋白的分子量分别为73、65、62、54、18kD等5种。高活力种胚合成的热激蛋白在最上高于低活力种子,高活力种胚合成的特异性热激蛋白56kD可以作为衡量种子活力的指标。双向电泳表明高低活力种子间热激蛋白的合成有更多质上的差异。 相似文献
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人工老化处理的卷心菜种子的热激蛋白合成 总被引:5,自引:0,他引:5
高活力卷心菜种子蛋白质合成速率比中等活力和低活力种子高很多。热激处理(42℃)下,蛋白质合成显著下降,但高活力种子的蛋白质合成能力仍然显著高于中等和低活力种子。高活力和中等活力种子主要合成分子量为70
kD和一些小分子量的热激蛋白。在低活力种子中检测不到热激蛋白的合成。4种热激蛋白(1种HSP90和3种HSP70)的Western
blot检测结果表明,只有1种热激蛋白(HSP70)与种子活力有关。 相似文献
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人工老化自理的卷心菜种子的热激蛋白合成 总被引:3,自引:0,他引:3
高活力卷心菜种子蛋白质合成速率比中等活力和低活力种子高很多。热激处理(42℃)下,蛋白质合成显著下降,但高活力种子的蛋白质合成能力仍然显著高于中等和低活力种子。高活力和中等活力种子主要合成分子量为70kD和一些小分子量的热激蛋白。在低活力种子中检测不到热激蛋白的合成。4种热激蛋白(1种HSP90和3种HSP70)的Western blot检测结果表明,只有1种热激蛋白(HSP70)与种子活力有关。 相似文献
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植物逆激蛋白的同源性及其基因表达与调控 总被引:4,自引:0,他引:4
植物逆激蛋白的同源性及其基因表达与调控汤学军,傅家瑞(中山大学生命科学学院,广州510275)关键词逆激蛋白,同源性,基因表达植物生活的“不动性”使其不可避免地要受到各种逆境的冲击,就是在其生活史中也会经历一些类似逆境的生理过程,如种子萌发初期的浸水... 相似文献
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热激蛋白60作为分子伴侣家族中的重要成员,在蛋白质的运输、组装以及折叠等方面起到重要的作用。利用离子交换层析和凝胶过滤层析两步纯化方法,从霞水母刺丝囊细胞中分离到热激蛋白60。SDS-PAGE结果显示,在分子量为60kDa处显示为单一清晰的蛋白条带,并且通过N末端测序进行鉴定,其序列为APKEIKFGADAKSLM与热激蛋白60相吻合;此外,还利用ELISA法对其进一步确定,同时对分离过程的热激蛋白60的回收率进行了测定。该方法为进一步研究霞水母热激蛋白60的功能及其应用奠定了基础。 相似文献
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Heat shock response of germinating embryos of wheat : effects of imbibition time and seed vigor 总被引:2,自引:2,他引:0
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Seeds frequently face a hostile environment during early germination. In order to determine whether seeds have evolved unique mechanisms to deal with such environments, a survey of the heat shock response in isolated embryos of wheat (Triticum aestivum L.) was undertaken. Embryos simultaneously heat shocked and labeled following several different periods of prior imbibition up to 12 hours synthesized many groups of heat shock proteins (hsps) typical of other plant and animal systems. Also, five developmentally dependent hsps, present only in treatments imbibed less than 6 hours prior to heat shock, were detected. These proteins have relative molecular masses of 14, 40, 46, 58, and 60 kilodaltons. One of the developmentally dependent hsps is among the most highly labeled hsps found in early imbibed embryos. The possibility that this protein is the Em protein is discussed. The hypothesis that the capacity for hsp synthesis is affected by seed vigor was also tested. The heat shock responses of embryos from two high and two low vigor seed lots were compared using one- and two-dimensional electrophoresis of labelled protein extracts. The results indicate that both of the low vigor lots tested had weaker heat shock responses than their high vigor counterparts overall. Not all hsps were relatively less abundant in low vigor embryos. The developmentally dependent hsps showed little relationship to vigor. Some of the developmentally dependent hsps were actually made in greater amounts, relative to other proteins, in the low vigor seed lots. The results presented here demonstrate that imbibing embryos are capable of expressing an enhanced heat shock response, and that this response is related to seed vigor. 相似文献
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Alfalfa, the most widely grown leguminous crop in the world, is generally exposed to severe salinity stress in Tunisia, notably affecting its germination performance. Toward a better understanding of alfalfa seed vigor, we have used proteomics to characterize protein changes occurring during germination and osmopriming, a pretreatment that accelerates germination and improves seedling uniformity particularly under stress conditions. The data revealed that germination was accompanied by dynamic changes of 79 proteins, which are mainly involved in protein metabolism, cell structure, metabolism, and defense. Comparative proteomic analysis also revealed 63 proteins specific to osmopriming, 65 proteins preferentially varying during germination, and 14 proteins common to both conditions. Thus, the present study unveiled the unexpected finding that osmopriming cannot simply be considered as an advance of germination-related processes but involves other mechanisms improving germination such as the mounting of defense mechanisms enabling osmoprimed seeds to surmount environmental stresses potentially occurring during germination. The present results therefore provide novel avenues toward understanding the mechanisms of invigoration of low vigor seeds by priming treatments that are widely used both in commercial applications and in developing countries (on farm seed priming) to better control crop yields. 相似文献
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Zhou Y Chen H Chu P Li Y Tan B Ding Y Tsang EW Jiang L Wu K Huang S 《Plant cell reports》2012,31(2):379-389
In plants, small heat shock proteins (sHSPs) are unusually abundant and diverse proteins involved in various abiotic stresses,
but their functions in seed vigor remain to be fully explored. In this study, we report the isolation and functional characterization
of a sHSP gene, NnHSP17.5, from sacred lotus (Nelumbo nucifera Gaertn.) in seed germination vigor and seedling thermotolerance. Sequence alignment and phylogenetic analysis indicate that
NnHSP17.5 is a cytosolic class II sHSP, which was further supported by the cytosolic localization of the NnHSP17.5-YFP fusion
protein. NnHSP17.5 was specifically expressed in seeds under normal conditions, and was strongly up-regulated in germinating seeds upon heat
and oxidative stresses. Transgenic Arabidopsis seeds ectopically expressing NnHSP17.5 displayed enhanced seed germination vigor and exhibited increased superoxide dismutase activity after accelerated aging treatment.
In addition, improved basal thermotolerance was also observed in the transgenic seedlings. Taken together, this work highlights
the importance of a plant cytosolic class II sHSP both in seed germination vigor and seedling thermotolerance. 相似文献
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种子活力与生物膜的研究现状 总被引:7,自引:0,他引:7
种子活力与生物膜的结构和功能密切相关.活力高的种子,膜结构比较完整,在吸水时,膜系统恢复的速度较快,并且修复的较完善.研究表明,超干和引发处理可使膜结构得到保持与修复,很多种类的物质参与了膜结构的保护,例如可溶性糖、蛋白质(包括酶)、两性分子、Ca2 、多胺及其他非酶促自由基清除系统等,保护物质协同作用,稳定膜脂及膜蛋白的结构,保持膜系统的完整性,使膜功能得以正常发挥,强化了种子活力. 相似文献
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种子活力与生物膜的结构和功能密切相关。活力高的种子,膜结构比较完整,在吸水时,膜系统恢复的速度较快,并且修复的较完善。研究表明,超干和引发处理可使膜结构得到保持与修复,很多种类的物质参与了膜结构的保护,例如可溶性糖、蛋白质(包括酶)、两性分子、Ca2+、多胺及其他非酶促自由基清除系统等,保护物质协同作用,稳定膜脂及膜蛋白的结构,保持膜系统的完整性,使膜功能得以正常发挥,强化了种子活力。 相似文献
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Proteomic investigation of the effect of salicylic acid on Arabidopsis seed germination and establishment of early defense mechanisms 总被引:5,自引:0,他引:5
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The influence of salicylic acid (SA) on elicitation of defense mechanisms in Arabidopsis (Arabidopsis thaliana) seeds and seedlings was assessed by physiological measurements combined with global expression profiling (proteomics). Parallel experiments were carried out using the NahG transgenic plants expressing the bacterial gene encoding SA hydroxylase, which cannot accumulate the active form of this plant defense elicitor. SA markedly improved germination under salt stress. Proteomic analyses disclosed a specific accumulation of protein spots regulated by SA as inferred by silver-nitrate staining of two-dimensional gels, detection of carbonylated (oxidized) proteins, and neosynthesized proteins with [35S]-methionine. The combined results revealed several processes potentially affected by SA. This molecule enhanced the reinduction of the late maturation program during early stages of germination, thereby allowing the germinating seeds to reinforce their capacity to mount adaptive responses in environmental water stress. Other processes affected by SA concerned the quality of protein translation, the priming of seed metabolism, the synthesis of antioxidant enzymes, and the mobilization of seed storage proteins. All the observed effects are likely to improve seed vigor. Another aspect revealed by this study concerned the oxidative stress entailed by SA in germinating seeds, as inferred from a characterization of the carbonylated (oxidized) proteome. Finally, the proteomic data revealed a close interplay between abscisic signaling and SA elicitation of seed vigor. 相似文献
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花生种子发育过程中活力的形成在时间上是不均衡的,果针入土后40d内活力水平很低,40d之后活力水平才急剧上升,这和贮藏蛋白迅速合成的时期吻合.随着贮藏蛋白质的合成和累积,由发育转向萌发时其被降解的速度加快,花生球蛋白被优先降解.和贮藏蛋白质其它组分相比较,花生球蛋白和种子活力有更密切的关系. 相似文献
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以16个小麦品种的种子为材料,通过标准发芽、逆境发芽和田间出苗试验,测定不同基因型小麦品种的种子活力,以不同发芽条件下种子活力指数的抗逆指数和田间出苗率作为衡量抗逆性的指标,利用主成分分析、聚类分析对种子活力进行综合评价.结果表明: 干旱胁迫、人工老化和冷浸胁迫3种逆境对种子活力都有一定的影响.人工老化抗逆指数和冷浸胁迫抗逆指数与田间出苗率呈显著正相关,干旱胁迫抗逆指数与田间出苗率的相关性不显著.通过主成分分析和聚类分析将16个小麦品种划分为3类、豫农949、豫麦49-198、鲁原502、郑育麦9987、石麦21、山农23号、石新828为高活力品种;许农5号、豫农982、唐麦8号、济麦20、济麦22、济南17号、山农20为中活力品种;长4738和轮选061属低活力品种. 相似文献
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盐碱地高盐分会降低种子活力、抑制萌发出苗,严重制约盐碱地区花生生产和产业发展。种子萌发过程中物质代谢对种子发芽及植株形态建成至关重要,逐渐成为评价种子活力和品质的重要指标。以不同萌发期花生种子为研究对象,利用生理指标和高效液相色谱串联质谱(LC-MS/MS)分析方法,研究了盐胁迫下花生种子不同萌发期主要营养物质含量和差异代谢物的变化。种子吸水萌发促进了脂肪、蛋白质、可溶性糖代谢,随萌发时间延长,脂肪和可溶性糖含量逐渐降低,可溶性蛋白质含量呈先降后升的变化趋势。主成分分析和偏最小二乘法判别分析表明盐胁迫与对照组间代谢物差异较大,暗示盐胁迫对花生种子萌发期物质代谢影响较大。利用VIP值分析和KEGG pathway预测分析显示:正常条件下,花生种子吸水膨胀期的差异代谢物较少,未鉴定到富集的KEGG pathway;而胚根伸长期差异代谢物主要富集于12个KEGG pathway,表明萌发后期物质代谢较前期旺盛。盐处理显著提高多种差异代谢物表达水平,其中渗透保护物甜菜碱和脯氨酸差异明显;另外,盐胁迫下吸水膨胀和胚根伸长两时段的差异代谢物显著增多,分别富集到26和31个KEGG pathway。盐胁迫显著促进了能量代谢、甘油磷脂代谢、谷胱甘肽代谢以及芥子油苷生物合成途径等相关通路,推测其与盐胁迫下花生种子萌发期抗逆有关。甜菜碱和脯氨酸可能是花生种子萌发期适应盐胁迫的关键代谢物,甘油磷脂代谢、谷胱甘肽代谢以及芥子油苷生物合成等途径可能是重要的代谢调控通路。试验结果可为促进盐胁迫下花生种子萌发出苗探索新途径、新方法,以及提高盐碱地花生出苗率提供理论依据和参考价值。 相似文献