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1.
Injection of norepinephrine (NE) at a dose of 10 micrograms per testis caused the testis refractory in terms of ornithine decarboxylase (ODC) activity at 24 h. This desensitization was found to be both time and dose dependent. Injection with follicle stimulating hormone, luteinizing hormone, prostaglandin F2 alpha, cyclic AMP or epinephrine to norepinephrine desensitized testis caused stimulation of ODC activity. This indicates that the refractoriness caused by norepinephrine is specific to this agent alone. 相似文献
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Binding of radioactive alpha-difluoromethylornithine to rat liver ornithine decarboxylase 总被引:6,自引:0,他引:6
M. Lynn Pritchard James E. Seely Hannu Pösö Leonard S. Jefferson Anthony E. Pegg 《Biochemical and biophysical research communications》1981,100(4):1597-1603
Inactivation of rat liver ornithine decarboxylase by incubation with [5-14C]-α-difluoromethylornithine resulted in the covalent binding of radio-activity to the enzyme. The extent of binding correlated with the degree of inactivation and with the amount of enzyme present. The labeled protein eluted as a single peak which coincided exactly with the active enzyme when chromatographed on Sephadex G-200 and ran as a single band on polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate at a position corresponding to a M.W. of about 55,000. The stoichiometric binding of [5-14C]-α-difluoromethylornithine therefore provides a convenient method for quantitating ornithine decarboxylase protein and for determining the purity of preparations of the enzyme. Assuming that 1 molecule of the drug is needed to inactivate each sub-unit, it was calculated that after stimulation with thioacetamide ornithine decarboxylase represents about 0.00014% of the liver soluble protein. 相似文献
4.
Dace Viceps-Madore K.Y. Chen Hwei-Ru Tsou E.S. Canellakis 《Biochimica et Biophysica Acta (BBA)/General Subjects》1982,717(2):305-315
The minimum requirements for eliciting or enhancing ornithine decarboxylase activity (EC. 4.1.1.17); L-ornithine carboxylase) in neuroblastoma cells incubated in salts-glucose solutions have been investigated. These incubation conditions permit the study of changes in ornithine decarboxylase activity independently of the growth-associated reactions that occur in cell culture media (Chen, K.Y. and Canellakis, E.S. (1977) Proc. Natl. Acad. Sci. U.S.A. 74, 3791–3795). Ornithine decarboxylase activity can be elicited by a variety of asparagine and other amino acid analogs, including α-aminoisobutyric acid, that cannot participate in protein synthesis. Of the eleven asparagine analogs tested. is the most potent in eliciting ornithine decarboxylase activity and is equivalent to asparagine in this regard. Inclusion of polar groups into the asparagine molecule results in the loss of its ability to elicit ornithine decarboxylase activity. With the use of these analogs and of analogs of other amino acids it is shown that the rapid fall in ornithine decarboxylase activity that is noted following cycloheximide treatment may not be a consequence of the inhibition of protein synthesis. The rapid fall in ornithine decarboxylase activity is primarily due to the removal of the agent that elicits and stabilizes its activity. These results, the finding that α-amminoisobutyric acid stimulates ornithine decarboxylase activity and that sodium is required for the stimulation of ornithine decarboxylase activity are discussed in relation to the ‘A’ amino acid transport system. 相似文献
5.
P P McCann C Tardif P S Mamont 《Biochemical and biophysical research communications》1977,75(4):948-954
Low concentrations of putrescine (10?5M) blocked ornithine decarboxylase (ODC) in rat hepatoma (HTC) cells in culture, but the lower homologue of putrescine, 1, 3 diaminopropane, had no effect on ornithine decarboxylase at 10?5M. Higher concentrations of both putrescine and 1, 3 diaminopropane induced approximately the same amount of soluble ODC antizyme type inhibitor. When concentrated dialyzed supernatants of cells grown in 10?5M putrescine were treated with 250 mM NaCl and chromatographed on a superfine Sephadex G-75 column, both ODC and inhibitor were recovered. Spermidine, spermine and cadaverine also induced the inhibitor suggesting a low specificity of induction by amines. 相似文献
6.
The biological activity of 1α-hydroxyvitamin D2 has been determined in vitamin D-deficient rats. In the calcification of the rachitic epiphyseal plate, 1α-hydroxyvitamin D2 is more active than 25-hydroxyvitamin D3, while it is equally active in stimulating intestinal calcium absorption. On the other hand, it is much less active (one-third to one-fifth) than 25-hydroxyvitamin D3 in the mobilization of calcium from bone. In both the intestinal and bone responses, 1α-hydroxyvitamin D2 (312 pmol) is active in nephrectomized rats while 25-hydroxyvitamin D3 is not. 相似文献
7.
John S. Heller Dimitris A. Kyriakidis Evangelos S. Canellakis 《Biochimica et Biophysica Acta (BBA)/General Subjects》1983,760(1):154-162
The purification of the antizymes to ornithine decarboxylase of Escherichia coli to homogeneity is detailed. An acidic component, pI 3.8, and two basic histone-like proteins, pI above 9.5, are described. The two latter proteins constitute approximately 90% of the total antizyme activity. 相似文献
8.
Fasting newborn and growing young rats, though capable of synthesizing liver glycogen when fed, are, unlike adult fasted animals, insensitive to glucocorticoid stimulation of the rate of glucose and lactate incorporation into glycogen. Hormone resistance parallels a decreased liver capability for the synthase b to a conversion reaction up to 2 days after birth, after which the b to a transformation becomes adult type in nature. A comparison of the level of glucose 6-phosphate in liver to the effect of the activator on the synthase activity from newborn rat shows that the enzyme has a greater affinity toward the activator than comparable enzyme from the adult, suggesting the presence of an intermediate metabolite-regulated form of synthase in neonatal liver. 相似文献
9.
A continuous spectrophotometric method suitable for the determination of the activities of several peroxisomal oxidases in rat tissue homogenates is described. The assay involves the continuous spectrophotometric measurement of the reaction product, H2O2, by coupling it to the reduction of a chromogen, o-dianisidine, with horseradish peroxidase. Catalase interference was overcome using azide to inhibit its activity and a H2O2 standard curve used to quantitate oxidase activity in terms of microkatals per milliliter of enzyme. 相似文献
10.
The mutagenic effect of 1,1-dimethylhydrazine (UDMH) was studied in the liver perfusion/cell culture system. Male Wistar rats, fed a selenium-deficient diet with or without selenium supplementation in the drinking water, were used as liver donors. UDMH caused an increased mutation frequency in Chinese hamster V79 cells exposed in the perfusate. The effect was statistically significant with both selenium-deficient and selenium-supplemented livers. With selenium-deficient livers, a significant mutagenic effect was also obtained when V79 cells were treated with bile collected after the administration of UDMH. Bile flow and bile acid excretion were not affected by UDMH treatment of selenium-deficient or selenium-supplemented livers. There was a tendency towards reduced C-oxygenation of N,N-dimethylaniline in microsomes from selenium-deficient livers perfused with UDMH. The lactate/pyruvate ratio in the perfusate was increased by UDMH, the effect being more pronounced with selenium-deficient than selenium-supplemented livers. 相似文献
11.
We have shown that an acidic phosphoprotein phosphatase (APP-ase) has a different pattern of postnatal maturation in the spleen, thymus and liver of rats and mice. The APP-ase activity increases during the first eight months of postnatal life in the spleen of rats (when it attains an 8--10 times higher value than at birth) and up to the sixth month of life in the spleen of mice. It increases considerably during the first two weeks of postnatal life in the thymus of rats and mice; in the liver of rats it reaches maximum activity before birth, but continues to increase up to the sixth month of postnatal life in the liver of mice. The results show also that the APP-ase from the spleen, thymus and liver of rats is equally active in dephosphorylating ATP and phenyl phosphate during the whole life span of rats, but not in relation to beta-glycerol phosphate. After analyzing its substrate specificity, its pH dependence in relation to different substrates, its kinetic properties, as well as its behaviour towards ascorbic acid and different inhibitors (sodium tungstate and sodium molybdate, L-tartrate, L-phenylalanine and L-cysteine) we have come to the conclusion that the rat spleen APP-ase is different from "nonspecific" acid and alkaline phosphatases and very similar to the EC 3.1.3.16 acid phosphoprotein phosphatase. 相似文献
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K Noguchi T Hattori T Igarashi K Ueno T Satoh H Kitagawa H Hori T Shibata S Inayama 《Life sciences》1985,37(7):625-633
A comparative study of the effect of misonidazole and novel radiosensitizers on glutathione (GSH) levels and related enzyme activities in isolated rat hepatocytes was performed. Incubation of hepatocytes with 5 mM radiosensitizers led to a decrease in the intracellular GSH level. The most pronounced decrease in cellular GSH was evoked by 2,4-dinitroimidazole-1-ethanol (DNIE); after incubation for only 15 min, GSH was hardly detected. DNIE-mediated GSH loss was dependent upon its concentration. DNIE reacted with GSH nonenzymatically as well as with diethylmaleate, while misonidazole and 1-methyl-2-methyl-sulfinyl-5-methoxycarbonylimidazole (KIH-3) did not. Addition of partially purified glutathione S-transferase (GST) did not enhance DNIE-mediated GSH loss in a cell-free system. DNIE inhibited glutathione peroxidase (GSH-Px), GST, and glutathione reductase (GSSG-R) activities in hepatocytes, while misonidazole and KIH-3 did not. GSH-Px activity assayed with H2O2 as substrate was the most inhibited. Inhibition of GSH-Px activity assayed with cumene hydroperoxide as substrate and GST was less than that of GSH-Px assayed with H2O2 as substrate. GSSG-R activity was decreased by DNIE, but not significantly. Incubation of purified GSH-Px with DNIE resulted in a little change in the activity when assayed with H2O2 as substrate. 相似文献
14.
Thomas J. Wincek Floyd W. Sweat 《Biochimica et Biophysica Acta (BBA)/General Subjects》1976,437(2):571-576
The results reported here show some characteristics of adenylate cyclase (EC 4.6.1.1) derived from homogenates of rat spleen, and describe the in vitro stimulation of this enzyme by prostaglandins, nucleotides, and F− under conditions where cyclic nucleotide degradative pathways are effectively inhibited.Particulate fractions from rat spleen homogenates contain high adenylate cyclase activities, and the highest specific activity is recovered in a particulate fraction prepared by low speed (1200 × g) centrifugation. Activity found in all particulate fractions is stimulated by fluoride, prostaglandins E1 and E2, catecholamines, and purine nucleotides. No stimulation is caused by prostaglandins F1α and F2α. Stimulation by prostaglandin E1 or E2 is augmented by GTP and other purine nucleotides, and stimulation by the combination of GTP and prostaglandin E1 is equal to that caused by optimal fluoride concentrations. Stimulation c caused by L-isoproterenol is additive to that caused by GTP but is not increased by GTP. 相似文献
15.
The suppressive effects of anti-allotype antibody on splenic lymphocytes of adult (>3 months of age) and newborn (1-week-old) rabbits were compared in vitro. An approximately 10-fold lower concentration of anti-b4 sufficed to modulate completely membrane-bound b4 immunoglobulin (Ig) when newborn cells were pulse-treated for 2 or 24 hr than when adult cells were tested. In contrast to splenic lymphocytes from adults, which regenerated most of the original proportion of b4+ cells in culture following treatment with up to 300 μg of anti-b4 for 24 hr (4), immature lymphocytes were susceptible to irreversible modulation of membrane-bound Ig even when lower concentrations of anti-b4 were used. However, under conditions permitting reversible modulation of membrane b4, lymphocytes of newborns regenerated the membrane product more rapidly than did adult cells. Both mature and immature splenocytes were shown to be capable of some level of b4 synthesis even in the continuing presence of anti-b4. No evidence for susceptibility to complement-mediated killing of newborn spleen cells by anti-allotypic antibodies was obtained. The observations reported here support the concept that the greater sensitivity of B lymphocytes from the newborn to succumb to irreversible suppressive effects by anti-allotype antibody plays a significant role in the restriction for induction of allotype suppression to the perinatal period. 相似文献
16.
Activation of the template activity of isolated rat liver nuclei for DNA synthesis and its inhibition by NAD 总被引:6,自引:0,他引:6
The template activity of isolated rat liver nuclei for DNA synthesis assayed with DNA polymerase was found to be dependent upon the presence of Ca2+ or Mg2+ in the incubation medium. DNA was prepared from isolated nuclei subjected to conditions which activated the template and centrifuged in an alkaline sucrose gradient. The distribution profile showed that smaller fragments were formed, suggesting enhancement of endonucleolytic activity. When isolated nuclei were incubated with NAD to induce poly(adenosine diphosphate ribose) formation and were subjected to the activation conditions, the template for DNA synthesis remained unchanged. The distribution profile in an alkaline sucrose gradient of DNA prepared from these nuclei and control nuclei was identical. The present findings suggest that the template-activating system for DNA synthesis was blocked when isolated nuclei were treated with NAD . 相似文献
17.
Effect of adrenocorticotrophic hormone on the activity of sterol ester hydrolase from rat brain 总被引:1,自引:0,他引:1
J Arnaud O Nobili J Boyer 《Biochemical and biophysical research communications》1981,100(3):1167-1172
Adrenocorticotrophic hormone (ACTH) stimulates in vitro the hydrolysis of oleoylcholesterol by a sterol ester hydrolase from rat brain synaptosomes. The stimulatory effect involves an alkaline shift of the pH optimum of catalysis and culminates at pH 6 with a 15 to 20-fold increase in lipolytic rates. The effect requires trace amount of organic solvent in the substrate emulsion and is dependent on the NH2-terminal sequence extending through the basic amino acid residues at positions 15–18. The hormonal stimulation is decreased when a lipid-depleted preparation is used as enzyme source, and fully restored upon addition of lecithin. The results raise the possibility that ACTH may have a neuro-hormonal role in brain via modulation of local lipolytic processes. 相似文献
18.
The possibility that the transient rise in rat ovarian ornithine decarboxylase (ODC) activity and the associated increase in putrescine which occur under luteinizing hormone control late in proestrus have an essential role in ovarian function has been tested using DL-α-difluoromethylornithine (DFMO) an irreversible inhibitor of ODC. Treatment with DFMO, 500 mg/kg s.c. at 12:00 h on the day of proestrus and 200 mg/kg, 6, 12 and 18 h thereafter completely suppressed both the rise in ovarian ODC activity and the associated increase in putrescine concentrations. However, ovulation took place normally under these conditions and the course of the resulting pregnancies was also normal. Similarly, combined treatment with DFMO and an inhibitor of S-adenosyl-L-methionine decarboxylase, 1,1-((methylethanediylidine)-dinitrilo) bis (3-aminoguanidine), 25 mg/kg, given at 10:00 h on the morning of proestrus failed to influence either ovulation or the subsequent period of gestation. These data provide no support for a functional role of the pre-ovulatory rise of ODC in rat ovary in the major peri-ovulatory events of that particular cycle, although they do not exclude effects on systems (e.g. steroidogenesis) not directly examined with our experimental approach. On the other hand, inhibition of ODC resulted in an increase in the number of uterine implantation sites following mating at the next proestrusestrus 4 days later. These data would support the previously expressed view that ODC may be associated with the gonadotrophin-induced initiation of follicular development for ovulation in the succeeding cycle. Moreover, since inhibition of the enzyme resulted in facilitation of the process, the normal physiological function of ODC, and/or the putrescine generated through its action, would appear to be inhibitory. 相似文献
19.
Effects of cultured astroglia on the survival of neonatal rat retinal ganglion cells in vitro 总被引:2,自引:0,他引:2
Retinal ganglion cells (RGC), as identified by retrograde horseradish peroxidase (HRP) labeling technique, were cultured in a minimal medium; only 20% of them survived after 16 hr in vitro. However, superior colliculus-conditioned medium was capable of supporting 100% of RGC over this assay time; enhanced neurite expression also was evident. It was decided to investigate whether glial cells within the superior colliculus may provide a soluble factor capable of supporting RGC. Glial-conditioned medium prepared over monolayers of either predominantly flat astrocytes (relatively immature) or predominantly process-bearing (mature) astrocytes failed to maintain RGC. The possibility that astrocytes may provide support for RGC via membrane contact was then investigated. Dissociated retinae were grown on monolayers consisting primarily of either flat or process-bearing astrocytes. Cultures rich in flat astrocytes maintained over 70% of RGC originally present, and many of them exhibited extensive neurite outgrowth and elongation. Process-bearing astrocytes were unable to support RGC survival. Immature astroglial cells may therefore support RGC via glial-neuronal interaction. 相似文献
20.
Intact nuclei were isolated in high yield from enriched fractions of immature and mature guinea pig granulocytic leukocytes. These nuclei were used to determine whether any changes in synthesis and content of nuclear proteins accompany the striking increase in chromatin condensation and the nuclear lobation which occur during granulocyte maturation. The results indicate that the synthesis of nuclear proteins and the nuclear RNA content decrease markedly during granulocyte maturation. The incorporation of l-[U-14C]leucine into the acid-soluble histone-rich fraction of chromatin from immature cells is about 25 times that of mature cells, and the incorporation into the acid-insoluble, nonhistone proteins of chromatin from immature cells is about 6 times that of mature cells. It appears that there is very little quantitative change with respect to the protein components of nuclei from immature and mature granulocytic leukocytes. No significant differences in the amounts of histone, nonhistone protein, or phosphoprotein between nuclei of immature and mature granulocytes could be detected. No major differences in gel electrophoretic patterns of histones or nonhistone proteins could be detected. The fact that the amount of the chromatin proteins remains relatively constant during cell maturation in spite of the pronounced decrease in the rate of synthesis suggests that the rate of turnover of these proteins decreases significantly as the maturation of granulocytic leukocytes proceeds. 相似文献