中国两种枝疣蛛记述(蜘蛛目:平腹蛛科)

记述采于太行山区的我国平腹蛛科枝疣蛛属2种蜘蛛：乔氏枝疣蛛Cladothela joannisi(Schenkel,1963)和新种扭曲枝疣株Cladothela bistorta sp.nov.。     

中国疣舟蛛属一新种：蜘蛛目：皿蛛科

本文记述１９９０年６月间采自吉林省长白山区的疣舟蛛属一新种,并对该种的雌雄蛛形态进行了描述,同时将该新种与其近似种进行了比较。模式标本均保存在白求恩医科大学生物教研室。     

雷氏大疣蛛毒素—Ⅱ的纯化与初步毒性研究

雷氏大疣蛛是我国最近鉴定的蜘蛛新种。雷氏大疣蛛毒素－Ⅱ就是以其粗毒为材料,利用阴、阳离子交换层析和反相HPLC分离得到并命名的一种新型神经毒素肽,根据质谱分析得知它的相对分子质量为3021．56;通过初步毒性研究,证明它是一个神经毒素。     

中国枝疣蛛属一新种和一新纪录种(蜘蛛目,平腹蛛科)

记述了平腹蛛科枝疣蛛属1新种,宁明枝疣蛛Cladothela ningmingenisis sp.nov.和中国1新纪录种,小枝疣蛛Cladothela parva Kamura,1991.     

中国微蛛三新种和一新纪录（蜘蛛目：皿蛛科）

本文记述采自长白山区皿蛛科微蛛亚科３新种和１新纪录种。新种包括：钩镰蛛Ｄｒｅｐａｎｏｔｙｌｕｓ ａｄｕｎｃｕｓ ｓｐ．ｎｏｖ．，圆双突蛛Ｔｉｂｉｏｐｌｏｉｄｅｓ ｃｙｃｌｉｃｕｓ ｓｐ．ｎｏｖ．和具结荫湿蛛Ｈｉｌａｉｒａ ｔｕｂｅｒｃｕｌｉｆｅｒａ ｓｐ．ｎｏｖ．。模式标本均保存于白求恩医科大学生物教研室。     

颜氏大疣蛛毒腺转录组学研究

颜氏大疣蛛Macrothele yani个体大、毒性强、易取毒,是农林生态系统中害虫的重要天敌,也是天然药物研究工作者进行新药挖掘和毒液开发利用的重要选择对象。本研究利用高通量测序平台Illumina Hiseq 2000对颜氏大疣蛛雌雄成体的毒腺进行转录组测序和生物信息学分析,共获得转录组样本数据37.8 G,总计301 024条unigenes,总长度170 512 372 bp,最短201 bp,最长36 105 bp,平均长度566 bp,GC含量平均值为38.17%,N50长度为705 bp。将unigenes序列与NR（非冗余蛋白序列数据库）、String（蛋白质相互作用数据库）、Swiss-prot（蛋白质序列数据库）、Pfam（蛋白质家族数据库）、GO（基因本体论）、KOG（真核生物蛋白质直系同源数据库）、KEGG（京都基因与基因组百科全书）数据库进行比对（e≤10^-10）,分别获得31 409、6 549、11 817、8 216、11 480、7 804条unigenes注释。通过生物信息学对雌雄颜氏大疣蛛unigenes进行表达量分析,筛选高表达量且高可信度的差异表达基因,并进行对这些差异基因进行GO和KEGG功能富集分析;与雄蛛相比,雌成体有205条unigenes表达量上调,113条unigenes表达量下调。最后在所有的转录本数据中共比对到68条毒素相关序列。本研究获得的颜氏大疣蛛转录组信息,为颜氏大疣蛛的功能基因挖掘提供了重要的信息资源。     

中国南方漏斗蛛三新种（蜘蛛目：漏斗蛛科）

本文记述神农架地区隙蛛属和蟋蛛属的蜘蛛三新种：珍珠隙蛛Ｃｏｅｌｏｔｅｓ ｂａｃｃａｔｕｓ ｓｐ．ｎｏｖ．;距形隙蛛Ｃｏｅｌｏｔｅｓ ｃａｌｃａｒｉｆｏｒｍｉｓｓｐ．ｎｏｖ．;象牙蟠蛛Ｃｉｃｕｒｉｎａ ｅｂｕｒｎｕｓｓｐ．ｎｏｖ．。     

雷氏大疣蛛毒液对人肝癌HepG2细胞p21基因表达的影响

本文研究了雷氏大疣蛛毒液对人肝癌细胞株HepG2增殖抑制作用及其分子机制。采用XTT法观察到雷氏大疣蛛毒液剂量依赖抑制HepG2细胞增殖;流式细胞仪检测发现,经过雷氏大疣蛛毒液作用的HepG2细胞周期发生明显的选择性改变;RT-PCR方法检测到p21基因表达增强;Western blot检测发现,p21蛋白表达增加。结果提示,雷氏大疣蛛毒液抑制人肝癌细胞HepG2增殖的可能机制之一是使p21基因和蛋白表达增加,G2IM细胞周期被阻滞,从而诱导细胞凋亡。     

中国管巢蛛属六新种(蜘蛛目:管巢蛛科)

本文记述管巢蛛属Ｃｌｕｂｉｏｎａ６新种,即环管巢蛛Ｃｌｕｂｉｏｎａｃｉｒｃｕｌａｔａｓｐ．ｎｏｖ．,双齿管巢蛛Ｃｌｕｂｉｏｎａｄｉｄｅｎｔａｔａｓｐ．ｎｏｖ．,丝歧管巢蛛Ｃｌｕｂｉｏｎａｆｉｌｏｒａｍｕｌａｓｐ．ｎｏｖ．,异管管巢蛛Ｃｌｕｂｉｏｎａｈｅｔｅｒｏｄｕｃｔａｓｐ．ｎｏｖ．,曲管巢蛛Ｃｌｕｂｉｏｎａｔｏｒｔｕｏｓａｓｐ．ｎｏｖ．,横列管巢蛛Ｃｌｕｂｉｏｎａｔｒａｎｓｖｅｒｓａｓｐ．ｎｏｖ．。     

吉林省长白山区鞭突蛛属一新种（蜘蛛目：皿蛛科：微蛛亚科）

本文记述采自我国吉林省长白山区的鞭蛛属一新种,即腹斑鞭突蛛Ｔｒｉｃｈｏｎｃｕｓ ｍａｃｕｌａｔｕｓ ｓｐ．ｎｏｖ．本文测量单位为ｍｍ,模式标本均保存在白求恩医科大学生物教研室。     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.     

肝癌中HBV和HCV基因和抗原的分布及意义

采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细     

Selection with two alleles and complete dominance

For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment.