Correlation between antibiotic sensitivity testing by conventional and conductivity measurements

A high correlation in the susceptibilities of 44 strains isolated from clinical material to a variety of antimicrobial agents was found between the minimum inhibitory concentration and conductivity measurements. Some discrepancy was found for vancomycin and teicoplanin when tested against 21 strains of staphylococci. Similarly, discrepancies were seen with azlocillin, chloramphenicol and gentamicin when tested against strains of aerobic and facultatively anaerobic Gram-negative bacilli. In the majority of cases in which discrepancies were noted, this reflected differences in relative, and not absolute, susceptibility. With five strains of Pseudomonas however, there was a poor correlation with three of four antibiotics studied. The advantage of this technique is that sensitivities are available within 4–6 h.     

blacCTX-M基因与氨基糖苷类抗生素耐药水平的相关性

目的分析β-内酰胺类耐药基因在肺炎克雷伯菌中的分布并评估这些基因对抗生素耐药水平的影响。方法选择76株肺炎克雷伯菌临床分离菌株,用K—B纸片法和MIC法检测抗生素的耐药性;用PCR法检测肺炎克雷伯菌株中β－内酰胺类抗生素的相关基因,RT—PCR的方法检测13-内酰胺类基因的表达。结果76株肺炎克雷伯菌中,ESBLs相关基因主要为SHV（45／76,59．21％）、TEM（27／76,35．53％）和CTX—M（38／76,50．00％）;携带SHV基因菌株对阿米卡星的耐药率（37．78％）明屁高于非携带SHV基因菌株（16．13％）（P〈0．05）。携带CTX—M基因（76．32％）和TEM基因（70．37％）的菌株对庆大霉素的耐药率明显高于不携带CTX—M基因（34．21％）和TEM基因（46．94％）的菌株（P〈0．05）。RT—PCR的结果显示,阿米卡星和庆大霉素耐药株的CTX—M基因mRNA的表达率均明显高于敏感株。结论β-内酰胺类基凶与氨基糖苷类抗生素的耐药相关,CTX—M基因与肺炎克雷伯菌对氨基糖苷类抗生素的耐药水平相关。     

Correlation between butyrylcholinesterase variants and sensitivity to soman toxicity

Butyrylcholinesterase (BChE) is synthesized in the liver and found in high concentrations in blood plasma, liver, heart, pancreas, vascular endothelium, skin, brain white matter, smooth muscle cells and adipocytes. BChE is a non specific enzyme that hydrolyzes different choline esters (succinylcholine, mivacurium) and many other drugs such as aspirin, cocaine and procaine. The enzyme is also considered as a bioscavenger due to its ability to neutralize the toxic effects of organophosphorus compounds (nervous system fs agents) such as soman. BChE displays several polymorphisms that influence its serum activity; therefore they could determine the individual sensitivity to chemical nerve agents. In this study, we investigated the correlation between BChE variants and the degree of enzyme inhibition and reactivation after soman application on blood samples of 726 individuals. The blood samples of individuals expressing abnormal variants, were more sensitive to soman compared to variants of homozygotes and heterozygotes for U-allele. We found significant differences in the degree of enzyme reactivation between different variants (with and without U-presence).     

Correlation between insecticidal and antibiotic activities of Bacillus thuringiensis parasporal crystals

The work was concerned with studying the insecticide and antibiotic activities of solutions containing parasporal crystals of several Bacillus thuringiensis subspecies. The correlation coefficients of these values were determined. A direct correlation was established between the insecticide and antibiotic activities of solutions of parasporal crystals from several B. thuringiensis subspecies. Therefore, the quality of bacterial insecticides can be controlled by assaying the antibiotic activity of polypeptide crystals in biotechnology.     

Correlation between biomass and medium conductivity in suspension cultures of rice cells

Summary An intercept in the linear relationship between the biomass increase and decrease in medium conductivity was found in suspension cultures of rice cells. It was due to the. consumption of medium salts by the cells during the lag phase. An equation was established for accurate monitoring of the cell growth which aids understanding of the cell physiology especially during the initial stage of cell growth.     

女性生殖道支原体感染与不孕症的关系及药敏分析

目的探讨女性生殖道支原体感染与不孕症之间的关系及对11种抗生素的敏感率,指导临床明确诊断,合理用药。方法随机选择2007年至2009年来长治市妇幼保健院就诊的女性不孕症患者360例,进行宫颈分泌物支原体培养和药敏试验。结果 360例宫颈分泌物标本中检出解脲支原体(Uu)阳性者161例、人型支原体(Mh)阳性者3例、解脲支原体(Uu)+人型支原体(Mh)混合阳性者29例;对阳性标本都做了11种抗生素的药敏试验,其中交沙霉素敏感率为93.47%、美满霉素敏感率为92.00%、强力霉素敏感率为90.21%、克拉霉素敏感率为83.69%、甲砜霉素敏感率为73.91%、环脂红霉素敏感率为61.95%和阿奇霉素敏感率为53.26%等。结论女性不孕症伴随高发的生殖道支原体感染,支原体感染与不孕症可能有关;长治地区生殖道支原体对交沙霉素、美满霉素、强力霉素、克拉霉素、甲砜霉素敏感性较高,环脂红霉素、阿奇霉素次之,对红霉素、罗红霉素敏感性较低,对环丙沙星、左氧氟沙星敏感性很低。     

Correlation between the consumption of amino acids and the production of the antibiotic gallidermin by Staphylococcus gallinarum

The correlation between the consumption of amino acids and the production of the polypeptide antibiotic gallidermin by Staphylococcus gallinarum Tü 3928 was investigated by on-line determination of amino acids and pulse experiments. A prolonged production phase together with an increase in gallidermin formation of about 25% was obtained during pulse and fed-batch experiments with the amino acids glutamic acid, glycine, serine and threonine.     

Comparison of sensitivity between real-time detection of a TaqMan assay for Batrachochytrium dendrobatidis and conventional detection

A sensitive and quantitative TaqMan assay for the causative agent of chytridiomycosis in amphibians (Batrachochytrium dendrobatidis) has been developed and is routinely used in diagnostic laboratories. We assessed whether the real time detection of the TaqMan assay was as sensitive as the detection of the PCR product by agarose gel electrophoresis and ethidium bromide staining. We found, for practical purposes, that gel-based detection of the diagnostic fragment produced by means of the TaqMan assay or by conventional PCR that used a different polymerase and reaction mix was as sensitive as the real-time detection of the TaqMan assay. We recommend the qualified use of conventional PCR amplification combined with agarose gel electrophoresis and ethidium bromide staining for studies where only prevalence data are required, funding for equipment is limited or the acquisition of a real-time system is not cost effective.