THE USE OF TETRACHLORONITROBENZENE ISOMERS ON LETTUCE

Field experiments were carried out from 1951 to 1955 on winter lettuce plants grown in boxes under glass and in the open, and on commercial crops in unheated glasshouses. Each of the three isomers of tetrachloronitrobenzene, applied as 5% dusts at 1/4 and 1/2 oz. per square yard of bed, gave significant protection against Botrytis disease, but the 2:3:4:5 isomer was inferior to the other two isomers and they all delayed the hearting of the crop. The 2:3:4:5 isomer at both rates of application and the 2:3:5:6 and 2:3:4:6 isomers at 1/2 oz./sq.yd. delayed hearting of a commercial crop by more than 14 days and are not recommended at these rates. Local overdoses of fungicide should be avoided.     

THE USE OF TETRACHLORONITROBENZENE ISOMERS ON POTATOES

In laboratory and field experiments from 1952 to 1955, 2:3:5:6-tetrachloro-I-nitrobenzene (tecnazene) delayed the development of sprouts on treated potato tubers, and 2:3:4:6-TCNB retarded sprout development in two laboratory experiments. At the usual commercial rates of application the 2:3:4:5 isomer had no effect on sprout development and very little effect at higher rates. The crop from 2:3:5:6-TCNB-treated seed tubers usually contained a higher proportion of small tubers than that from untreated or 2:3:4:5-TCNB-treated seed. Marketable yield from 2:3:5:6-TCNB-treated seed was sometimes below that from untreated seed, but when this happened the yield from 2:3:4:5-TCNB-treated seed was higher.
Both isomers produced good control of dry rot, Fusarium caeruleum (Lib.) Sacc., on five varieties of potatoes, but did not produce a commercial control on Doon Star. To achieve control, the isomers had to be present at the time of infection. There was some evidence that the development of disease symptoms was delayed in treated, infected tubers. Since 2:3:4:5-tetrachloro-1-nitrobenzene has no sprout depressant action it merits further trial as a fungicidal treatment for seed potatoes.     

THE EFFECT OF LIGHT ON THE USE OF THE NITROGEN RESERVES OF GERMINATING SOYBEAN SEEDS

Light affects the mobilization and distribution of several of the storage components of the cotyledons of germinating soybean seeds. The nitrogen content of the cotyledons began to decrease during the first day of germination, continued through day 12 for plants in the light, and day 14 for those in the dark. Cotyledons from both treatments had lost about the same amount of nitrogen by day 14. Plants from both treatments lost about the same amount of dry weight by day 8, but those in the light had taken up nitrogen from the nutrient solution; while those in the dark showed no increase. The plants in the light had higher concentrations of soluble amino nitrogen in the cotyledons than did those in the dark, but the opposite was true for the seedling axis. Aspartate and its amide accounted for half or more of the total free amino acids in all parts of dark-grown plants at 6 and 14 days. In the light-grown plants aspartate and asparagine usually accounted for less than half of the total free amino acids in all plant parts except the cotyledons at 6 and 14 days. Total soluble amino acids were much lower in these plants than those in the dark, excepting the cotyledons.