Disc electrophoretic investigation of Coffea arabica and C. canephora: General protein and malate dehydrogenase of mature seeds

A preliminary disc electrophoretic investigation utilizing malate dehydrogenase and general protein banding patterns obtained from mature seeds revealed detectable differences among Coffea species, varieties and cultivars. Protein differences among seed samples from individual plants of the same variety were also implied. The analyzed protein data were presented as dendrograms and compared with published genetical and taxonomical data.     

A comparison of acid extracted globulin fractions and vicilin and legumin of Vicia faba

Two globulin fractions, obtained by extraction using acidic conditions, were characterized by their sedimentation properties, subunit compositions and     

Chemical data related to the tribal affinities of Hulsea and Arnica

The taxonomic placement of Hulsea is discussed in light of the distribution of pyrrolizidine alkaloids, sesquiterpene lactones, and polyacetylenic compounds in the Asteraceae.     

Distribution of triterpene alcohols in subcellular fractions from Calendula officinalis flowers

The distribution of the triterpene mono- and dihydroxy alcohols was investigated in the subcellular fractions of the flowers of Calendula officinalis. The triterpene monols were found mainly in the chromoplast fraction (68% of total) with smaller amounts in the cell debris, microsomal and supernatant fractions, the mitochondrial fraction was almost devoid of these compounds. Triterpene diols were present exclusively in the chromoplast fraction, 98% in the form of the 3-monoesters and 2% in the form of diesters. It is suggested that the hydroxylation of the triterpene monols to the corresponding diols proceeds in the chromoplasts and the esterified form of the monols is probably the substrate for this reaction.     

A chemotaxonomic study of Brassica oleracea with particular reference to its relationship to Brassica alboglabra

A chemotaxonomic study has been made of Brassica oleracea L., its horticultural varieties and Brassica alboglabra Bailey with reference to seed proteins, certain isoenzymes and isothcioyanates. The results of the investigation do not support separate species status in respect of B. alboglabra.     

Coumarins from Pterocaulon balansae and P. lanatum

Analysis of the petrol-diethyl ether extracts of Pterocaulon balansae and P. lanatum aerial parts afforded, besides puberulin, scopoletin-[2′     

Polysaccharase metabolism in dormant and germinating Stylosanthes humilis seeds

The levels of certain polysaccharase and glycosidase enzymes were followed during the germination of Townsville stylo seeds. Significant levels of inve     

Ent-norlabdane triols from Austroeupatorium inulaefolium

Two new ent-norlabdane triols were isolated from the above-ground parts of Austroeupatorium inulaefolium.     

Heliangolides from Leucanthemopsis pulverulenta

The structure of hispanolide (4,5-cis-3-oxogermacranolide) and three other related germacranolides, isolated from Leucanthemopsis pulverulenta, have been established by a series of chemical transformations and spectral data.     

Flavonoids in Parahebe and Veronica: a chemosystematic study

Flavone glycosides are the main flavonoid leaf constituents in the related genera Parahebe and Veronica (Scrophulariaceae), in agreement with former chemical studies of the family. In Parahebe there are groups of species in which there are mainly luteolin glycosides, and groups in which 6-hydroxyluteolin dominates. Small amounts of apigenin occur in many taxa. Glycosylation is usually in the 7-position but 4′- and 5-glycosides were also found. In Veronica a larger variety of flavone aglycones was found: e.g. luteolin, apigenin, chrysoeriol, tricin and three different 6-hydroxyflavones. They are often present in the plants in the form of glucuronides. Glycosylation is in the 7-or-5-position. Most species of both genera have a distinctive pattern of flavonoid glycosides in their leaves which can be used for identification. Populations of P. catarractae are an exception in showing three different patterns, but here the variety in flavone profiles corresponds to the pattern of morphological and geographic variation within this taxon. Anthocyanins are responsible for the blue, mauve and pink colours of the flowers in the two genera. In Veronica they are based on delphinidin, whereas in Parahebe catarractae on both delphinidin and cyanidin.     

Chromenes from ageratina riparia

The reinvestigation of the roots and aerial parts of Ageratina riparia afforded ten new chromenes, their structures being determined by spectral data and chemical transfomrations.     

Hirsutinolides from South African Vernonia species

The investigation of four South African Vernonia species afforded, in addition to known compounds, six ketohirsutinolides with different oxygen fun     

Aromatic compounds from Artemisia campestris subsp. Glutinosa

From the weakly acid fraction of the hexane extract from Artemisia campestris subsp. glutinosa, five new aromatics have been isolated. They hav     

Biosynthesis of oleanolic acid glycosides by subcellular fractions of Calendula officinalis seedlings

The synthesis of oleanolic acid 3β-d-glucuronoside from oleanolic acid and UDPGlcA has been demonstrated in cell-free preparations from C. officinalis seedlings. Moreover, the formation of more complex glycosides by successive additions of galactose and glucose to oleanolic acid glucuronoside was observed when cell-free preparations were incubated with UDPGal or UDPGlc. The consecutive steps of oleanolic acid glycosylation are localized in three different cellular compartments. The biosynthesis of the 3-glucuronoside takes place in the microsomes, the elongation of the sugar chain at C-3 of the aglycone proceeds in heavy membrane structures which are probably fragments of the Golgi complex while a cytosol enzyme(s) is involved in glucosylation of the C-17 carboxyl group of oleanolic acid.     

Ent-beyerene and ent-atisene diterpenes from viguiera insignis

The new diterpenes, ent-beyer-15-ene-12α,19-diol and ent-atis-13-en-3β,16α-diol, were isolated as minor constituents from the aerial part of Viguiera insignis. The structures were established by biomimetic transformation, spectroscopic means and comparison with closely related compounds.     

Diterpenoids from Baccharis tola

Erythroxylol-A, oleanolic acid, erythroxylol-A oxide, scopolatine, 4-hydroxy-3-methoxyacetophenone and two new diterpenes, ent-beyer-14-en-18-ol and 19-hydroxy-13-epimanoyl oxide, were isolated from B. tola.     

Oxidized kaurene derivatives from leaves of Solidago missouriensis and S. Rigida

Oxidized kaurene derivatives were isolated from the leaves of Solidago missouriensis and S. rigida and identified as kauran-16β-ol, kaur-16-en-19-oic acid and 7β-hydroxykaur-16-en-19-oic acid. The structure of the latter compound was determined by X-ray crystallographic analysis of its methyl ester.     

Prenylated p-coumarates from Werneria stuebelii

The aerial parts of Werneria stuebelii afforded in addition to β-cedrene and bicyclogermacrene eight derivatives of methyl p-coumarate, all being formed by prenylation. The structures were elucidated by their molecular formulae and their ¹H NMR spectra. The chemotaxonomy of the Andean genus Werneria is discussed briefly.     

Ent-Labdanes from Baccharis Sternbergiana

The aerial parts of Baccharis sternbergiana afforded six new ent-labdane derivatives, while those of a Baccharis tricuneata subspecies gave 12-acetoxytremetone.