共查询到20条相似文献,搜索用时 34 毫秒
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《Bioscience, biotechnology, and biochemistry》2013,77(4):642-647
The growth inhibition by nisin-producing lactococci against Bacillus subtilis and its application to soybean miso fermentation were investigated. Lactococcus lactis subsp. lactis IFO12007 (nisin-producing, salt-intolerant) rapidly proliferated to more than 109 cells/g in cooked soybeans without any excessive pH decrease. In spite of the mild decrease in pH, the growth of B. subtilis was completely inhibited; no living cells were detected in a soybean sample inoculated with 106 cells/g and incubated for 24 to 72 h. This Lc. lactis was applied to soybean miso fermentation as a starter culture. It produced high nisin activity (1.28×105 AU/g) in cooked soybean, resulting in the complete growth inhibition of B. subtilis, which had been inoculated at the beginning of the koji fermentation, throughout the process of miso production. Over-acidification, which is undesirable for miso quality, was successfully prevented simply by adding salt which killed the salt-intolerant Lc. lactis. Furthermore, the nisin activity in miso disappeared with aging. 相似文献
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Growth of the Bacillus subtilis wild type strain was severelyinhibited by mannosamine. This inhibitory effect was reversedby the addition of glucose or mannose. Growth in peptone mediumwas accompanied by the consumption of the added glucose andby the accumulation of acetoin. Glucose utilization and acetoinproduction were markedly repressed in the cells grown in thepresence of mannosamine. Growth in the presence of mannosaminewas accompanied by a relative decrease in a protein with themolecular weight of 70,000 as revealed by SDS polyacrylamidegel electrophoresis. Our results indicate that mannosamine'sinhibition of growth is due primarily to low glucose utilizationalthough other cellular metabolic activity also may be involved. (Received August 15, 1983; Accepted April 2, 1984) 相似文献
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Claudia S. López Horacio Heras Sandra M. Ruzal Carmen Sánchez-Rivas Emilio A. Rivas 《Current microbiology》1998,36(1):55-61
The envelope properties of B. subtilis cultures grown in LB and LBN hyperosmotic media (LB + 1.5 M NaCl) were compared. Since hypertonic cultures showed a Spo-phenotype,
a Spo-mutant grown in LB was also analyzed. LBN cultures showed extensive filamentation and presented different sensitivities
toward phage infection (φ29 and φ105), or antibiotics whose targets are at wall (lysozyme, penicillin G) or membrane level
(polymyxin B, phosphonomycin). Results of the biochemical composition revealed that during hyperosmotic growth, the cell wall
increased in thickness, and among the membrane lipids, glycolipid and cardiolipin increased in parallel with a decrease in
phosphatidylglycerol. The fatty acid composition was also modified, and an increase in saturated straight chain with a decrease
of saturated iso-branched fatty acids was observed. The increase of monounsaturated 18-1 (ω-9) fatty acid was probably related
to the absence of sporulation observed in hypertonic media, since its increase has been shown to inhibit the KinA sensor of
sporulation. The significance of the other wall and membrane composition variations (and hydrophobic surface properties) in
relation to the osmotic adaptation are discussed.
Received: 16 June 1997 / Accepted: 22 July 1997 相似文献
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Gabdrakhmanova LA Shakirov EV Balaban NP Sharipova MR Rudenskaia GN Kostrov SV Akimkina TV Leshchinskaia IB 《Mikrobiologiia》2000,69(5):653-659
A nutrient medium was elaborated for the efficient production of glutamyl endopeptidase by the recombinant Bacillus subtilis strain AJ73 bearing the Bacillus intermedius 3-19 glutamyl endopeptidase gene within a multicopy plasmid. Optimal concentrations of the main nutrients, peptone and inorganic phosphate, were found using a multifactor approach. To provide for active growth and efficient glutamyl endopeptidase production, the cultivation medium of the recombinant strain should be enriched in phosphorus, organic and inorganic nitrogen sources, and yeast extract. Complex protein substrates, such as casein and gelatin, enhanced the biosynthesis of glutamyl endopeptidase. At the same time, easily metabolizable carbon sources suppressed it. The production of glutamyl endopeptidase was stimulated by the bivalent cations Ca2+, Mg2+, and Co2+. 相似文献
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Summary Optimal concentrations of glucose, yeast extract and nutrient broth for the production of -lactamase by B. subtilis were predicted using a statistical experimental design and then tested. More yeast extract (13 vs. 1 g/L), less glucose (7.4 vs. 10 g/L), and less nutrient broth (12.6 vs. 15 g/L) were required to achieve high -lactamase activities (5700 U/L) instead of high cell growth rates (1.2 h-1). 相似文献
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Mercuric ions, as well as organomercuric ions and cadmium ions, can inhibit deoxyribonucleic acid-mediated transformation in Bacillus subtilis 168 without decreasing the viability of the total population. Differences in the inhibition of transformation by mercuric ions are identifiable on a temporal and concentration dependence basis. Sensitivity to low concentrations (9.2 x 10(-8) M) appears early in the uptake of deoxyribonucleic acid before the transformed markers have become insensitive to deoxyribonuclease. Resistance to "low concentrations" of Hg(2+) is kinetically indistinguishable from the requirement for magnesium in the transformation process. This inactivation is not reversed by the mercury-binding compound glutathione. Sensitivity to mercuric ions at a higher concentration (5.52 x 10(-7) M) occurs after the donor deoxyribonucleic acid has become insensitive to deoxyribonuclease. These complex interactions between mercuric ions and the process of transformation are discussed. 相似文献
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Inhibition of spore outgrowth in Bacillus subtilis by chloroquin 总被引:1,自引:0,他引:1
W C McDonald 《Canadian journal of microbiology》1967,13(5):611-613
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J N Reeve 《Journal of bacteriology》1974,119(2):560-563
A double mutant strain, Bacillus subtilis div IV B1 dal trp, has been constructed which grows as filamentous cells and minicells and requires d-alanine for growth. Removal of d-alanine from a growing population of cells resulted in cell bulging 25% of the cell length from one cell pole, followed by cell lysis. Little ultrastructural change in the cell envelope could be detected during bulge formation. 相似文献
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A 1-mg/ml amount of threonine (8.4 mM) inhibited growth and sporulation of Bacillus subtilis 168. Inhibition of sporulation was efficiently reversed by valine and less efficiently by pyruvate, arginine, glutamine, and isoleucine. Inhibition of vegetative growth was reversed by asparate and glutamate as well as by valine, arginine, or glutamine. Cells in minimal growth medium were inhibited only transiently by very high concentrations of threonine, whereas inhibition of sporulation was permanent. Addition of threonine prevented the normal increase in alkaline phosphatase and reduced the production of extracellular protease by about 50%, suggesting that threonine blocked the sporulation process relatively early. 2-Ketobutyrate was able to mimic the effect of threonine on sporulation. Sporulation in a strain selected for resistance to azaleucine was partially resistant. Seventy-five percent of the mutants selected for the ability to grow vegetatively in the presence of high threonine concentrations were found to be simultaneously isoleucine auxotrophs. In at least one of these mutants, the threonine resistance phenotpye could not be dissociated from the isoleucine requirement by transformation. This mutation was closely linked to a known ilvA mutation (recombination index, 0.16). This strain also had reduced intracellular threonine deaminase activity. These results suggest that threonine inhibits B. subtilis by causing valine starvation. 相似文献
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