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1.
Elizabeth Vierling 《Acta Physiologiae Plantarum》1997,19(4):539-547
In response to high temperature stress, plants express numerous small heat shock proteins (sHSPs) belonging to at least five
related gene families. in vitro studies suggest sHSPs act as molecular chaperones to prevent irreversible heat denaturation of other proteins. The diversity
of sHSPs in plants is unique among eukaryotes and makes it of interest to understand the origins of these proteins. sHSP-related
proteins have now been identified in 13 prokaryotes, and in many of these prokaryotes the sHSPs are heat-regulated as seen
higher plants. The prokaryotic sHSPs were analyzed by pairwise and mutliple sequence alignments with each other and with plant
sHSPs. The higher plant class I cytosolic sHSPs are shown to be most similar to a subset of the prokaryotic sHSPs, including
HSP 16.6 from the cyanobacterium Synechocystis. Genetic studies in this model cyanobacterium may provide insight into sHSP function in vivo, and into potential roles of sHSPs in higher plant cells. 相似文献
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Small heat shock proteins (sHSPs) are chaperones that are crucial in the heat shock response but also have important nonstress
roles within the cell. sHSPs are found in all three domains of life (Bacteria, Archaea, and Eukarya). These proteins are particularly
diverse within land plants and the evolutionary origin of the land plant sHSP families is still an open question. Here we
describe the identification of 17 small sHSPs from the complete genome sequences of five diverse algae: Chlamydomonas reinhardtii, Cyanidioschyzon merolae, Ostreococcus lucimarinus, Ostreococcus tauri, and Thalassiosira pseudonana. Our analysis indicates that the number and diversity of algal sHSPs are not correlated with adaptation to extreme conditions.
While all of the algal sHSPs identified are members of this large and important superfamily, none of these sHSPs are members
of the diverse land plant sHSP families. The evolutionary relationships among the algal sHSPs and homologues from bacteria
and other eukaryotes are consistent with the hypothesis that the land plant chloroplast and mitochondrion sHSPs did not originate
from the endosymbionts of the chloroplast and mitochondria. In addition the evolutionary history of the sHSPs is very different
from that of the HSP70s. Finally, our analysis of the algal sHSPs sequences in light of the known sHSP crystal structures
and functional data suggests that the sHSPs possess considerable structural and functional diversity.
Electronic supplementary material The online version of this article (doi: ) contains supplementary material, which is available to authorized users.
Reviewing
Editor: Dr. Rüdiger Cerff 相似文献
4.
Xiang-Qin Liu Jonathan P. Hosler John E. Boynton Nicholas W. Gillham 《Plant molecular biology》1989,13(4):385-394
Previous studies have identified a set of highly phosphorylated proteins of 23–25 kDa accumulated during normal embryogenesis of Zea mays L. and which disappear in early germination. They can be induced precociously in embryos by abscisic acid (ABA) treatment. Here the synthesis and accumulation of this group of proteins and their corresponding mRNAs were examined in ABA-deficient viviparous embryos at different developmental stages whether treated or not with ABA, and in water-stressed leaves of both wild-type and viviparous mutants.During embryogenesis and precocious germination of viviparous embryos the pattern of expression of the 23–25 kDa proteins and mRNAs closely resembles that found in non-mutant embryo development. They are also induced in young viviparous embryos by ABA treatment. In contrast, leaves of ABA-deficient mutants fail to accumulate mRNA in water stress, yet do respond to applied ABA. In water-stressed leaves of wild type plants the mRNAs are induced and translated into 4 proteins with a molecular weight and isoelectric point identical to those found in embryos.These results indicate that the 23–25 kDa protein set is a new member of the recently described class or proteins involved in generalized plant ABA responses.The different pattern of expression for the ABA-regulated 23–25 kDa proteins and mRNAs found in embryo and in vegetative tissues of viviparous mutants is discussed. 相似文献
5.
Daniela Wagner Jens Schneider-Mergener Christoph Forreiter 《Journal of Plant Growth Regulation》2005,24(3):226-237
Small heat shock proteins (sHSPs) are the most abundant stress proteins in plants. Usually not expressed under permissive
conditions, they can accumulate to more than 2% of the total cellular protein content during heat stress. At present several
points of evidence indicate that these proteins act as molecular chaperones by keeping partially denatured proteins in a folding-competent
state. In plants sHSPs are encoded by a multigene family, which can be segregated into several classes according to their
subcellular position and/or sequence homology. Curiously, two different classes appear in the cytoplasm. Their specific role
during heat shock remains elusive. Here we present some evidence that both classes of sHSPs enhance recovery of reporter protein
activity in the presence of HSP70. Applying peptide arrays prepared by SPOT synthesis and in situ analysis by confocal laser scanning microscopy, we could further show that the two classes of sHSP are attached to each other
and are able to interact with non-native proteins both in vivo and in vitro. Although both of the sHSPs act similarly as molecular chaperones, immunohistochemistry experiments support the hypothesis
that the two have different cellular functions in the development of heat-induced cytoplasmic heat shock granules under elevated
temperatures.
Daniela Wagner Deceased 24 Feburary 2004. 相似文献
6.
The FK506-binding proteins (FKBPs) belong to the peptidyl prolyl cis-trans isomerase (PPIase) family, and catalyse the rotation of the peptide bond preceding a proline. They are conserved in organisms from bacteria to man. In order to understand the function of plant FKBP isoforms, we have produced transgenic wheat plants overexpressing each of the two wheat FKBPs: wFKBP73 (which is expressed in young vegetative and reproductive tissues under normal growth conditions) and wFKBP77 (which is induced by heat stress). Transgenic lines overexpressing wFKBP77 at 25°C showed major morphological abnormalities, specifically relating to height, leaf shape, spike morphology and sterility. In these plants, the levels of hsp90 mRNA were over two fold higher than in controls, indicating a common regulatory pathway shared between wFKBP77 and Hsp90. Transgenic lines overexpressing wFKBP73 showed normal vegetative morphology, but the grain weight and composition was altered, corresponding to changes in amylase activity during seed development. 相似文献
7.
The small heat shock proteins (sHSPs) are a diverse family of molecular chaperones. It is well established that these proteins
are crucial components of the plant heat shock response. They also have important roles in other stress responses and in normal
development. We have conducted a comparative sequence analysis of the sHSPs in three complete angiosperms genomes: Arabidopsis thaliana, Populus trichocarpa, and Oryza sativa. Our phylogenetic analysis has identified four additional plant sHSP subfamilies and thus has increased the number of plant
sHSP subfamilies from 7 to 11. We have also identified a number of novel sHSP genes in each genome that lack close homologs
in other genomes. Using publicly available gene expression data and predicted secondary structures, we have determined that
the sHSPs in plants are far more diverse in sequence, expression profile, and in structure than had been previously known.
Some of the newly identified subfamilies are not stress regulated, may not posses the highly conserved large oligomer structure,
and may not even function as molecular chaperones. We found no consistent evolutionary patterns across the three species studied.
For example, gene conversion was found among the sHSPs in O. sativa but not in A. thaliana or P. trichocarpa. Among the three species, P. trichocarpa had the most sHSPs. This was due to an expansion of the cytosolic I sHSPs that was not seen in the other two species. Our
analysis indicates that the sHSPs are a dynamic protein family in angiosperms with unexpected levels of diversity.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
8.
Wheat vegetative nitrogen compositional changes in response to reduced reproductive sink strength 总被引:5,自引:0,他引:5
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N redistribution patterns and the N composition of vegetative tissues above the peduncle node of wheat (Triticum aestivum L.) plants with altered reproductive sink strength were evaluated to determine the role of vegetative storage proteins in the temporary storage of excess N destined for export. The degree of leaf senescence symptoms (loss of chlorophyll, total N, and ribulose-1,5-bisphosphate carboxylase/oxygenase) were initially reduced, but the complete senescence of vegetative tissues proceeded even for plants completely lacking reproductive sinks. Plants with 50% less sink strength than control plants with intact spikes redistributed vegetative N to the spike almost as effectively as the control plants. Plants without reproductive sinks exported less N from the flag leaf and had flag leaf blades and peduncle tissues with higher soluble protein and α-NH2 amino acid levels than control plants. An abundant accumulation of polypeptides in the soluble protein profiles of vegetative tissues was not evident in plants with reduced sink strength. Storage of amino acids apparently accommodates any excess N accumulated by vegetative tissues during tissue reproductive growth. Any significant role of vegetative storage proteins in the N economy of wheat is unlikely. 相似文献
9.
Molecular cloning and characterization of novel isoforms of potato ADP-glucose pyrophosphorylase 总被引:7,自引:0,他引:7
Ursula La Cognata Lothar Willmitzer Bernd Müller-Röber 《Molecular & general genetics : MGG》1995,246(5):538-548
ADP-glucose pyrophosphorylase (AGPase) is one of the major enzymes involved in starch biosynthesis in higher plants. We report here the molecular cloning of two cDNAs encoding so far uncharacterized isoforms (AGP S2 and AGP S3) of the potato enzyme. Sequence analysis shows that the two polypeptides are more homologous to previously identified large subunit polypeptides from potato and other plant species than to small subunit isoforms. This observation suggests that AGP S2 and AGP S3 represent novel large subunit polypeptides. agpS2 is expressed in several tissues of the potato plant, including leaves and tubers. Expression was stronger in sink leaves than in source leaves, indicating developmental regulation. In leaves, agpS2 expression was induced 2- to 3-fold by exogenous sucrose; therefore, agpS2 represents a new sucrose-responsive gene of starch metabolism. Expression of agpS3 was restricted to tubers: no agpS3 expression could be seen in leaves of different developmental stages, or when leaves were incubated in sucrose. Therefore, agpS3 represents the only AGPase gene so far characterized from potato, which is not expressed in leaves. Conversely, all four AGPase isoforms known from potato are expressed in tubers. 相似文献
10.
Cheon BY Kim HJ Oh KH Bahn SC Ahn JH Choi JW Ok SH Bae JM Shin JS 《Transgenic research》2004,13(6):541-549
Erythropoietin (EPO) is a glycoprotein used for curing human anemia by regulating the differentiation of erythroid progenitors and the production of red blood cells. To examine the expression of recombinant EPO in plants, pPEV-EP21, in which human epo cDNA under the control of the CaMV 35S promoter, was introduced into tobacco and Arabidopsisvia Agrobacterium tumefaciens-mediated transformation. The RNA expression level of epo in the transgenic lines was initially estimated by Northern blot analysis. Two transgenic lines, which exhibited a high expression level of epo mRNA determined by Northern analysis, were chosen for Western blot analysis to examine the production of EPO proteins. Those two lines, EP21-12 and EP21-14, revealed detectable bands on the immunoblot. Interestingly, constitutive expression of the human epo gene affected the morphologies in transgenic plants such that vegetative growth of transgenic tobacco was retarded, and male sterility was induced in transgenic tobacco and ArabidopsisThese authors contributed equally to this work 相似文献
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12.
Analysis and Phylogeny of Small Heat Shock Proteins from Marine Viruses and Their Cyanobacteria Host
Small heat shock proteins (sHSPs) are oligomeric stress proteins characterized by an α-crystallin domain (ACD) surrounded by a N-terminal arm and C-terminal extension. Publications on sHSPs have reported that they exist in prokaryotes and eukaryotes but, to our knowledge, not in viruses. Here we show that sHSPs are present in some cyanophages that infect the marine unicellular cyanobacteria, Synechococcus and Prochlorococcus. These phage sHSPs contain a conserved ACD flanked by a relatively conserved N-terminal arm and a short C-terminal extension with or without the conserved C-terminal anchoring module (CAM) L-X-I/V, suggested to be implicated in the oligomerization. In addition, cyanophage sHSPs have the signature pattern, P-P-[YF]-N-[ILV]-[IV]-x(9)-[EQ], in the predicted β2 and β3 strands of the ACD. Phylogenetically, cyanophage sHSPs form a monophyletic clade closer to bacterial class A sHSPs than to cyanobacterial sHSPs. Furthermore, three sHSPs from their cellular host, Synechococcus, are phylogenetically close to plants sHSPs. Implications of evolutionary relationships between the sHSPs of cyanophages, bacterial class A, cyanobacteria, and plants are discussed. 相似文献
13.
Sergei F. Krasnyanski Jagdeep Sandhu Leslie L. Domier Dennis E. Buetow Schuyler S. Korban 《In vitro cellular & developmental biology. Plant》2001,37(4):427-433
Summary Two different promoters, a cauliflower mosaic virus (CaMV) 35S promoter with a 5′-untranslated leader sequence from alfalfa
mosaic virus RNA4 (designated as CaMV 35S/AMV) and an E-8 fruit-ripening-specific promoter, were compared to evaluate their
effects on expression of the uidA reporter gene in transgenic tomato plants. In order to generate sufficient numbers of transgenic tomato plants, both a reliable
regeneration system and an efficient Agrobacterium transformation protocol were developed using 8-d-old cotyledons of tomato (Lycopersicon ecsulentum Mill. cv. Swifty Belle). Two sets of constructs, both derivatives of the binary vector pBI121, were used in transformation
of tomato whereby the uidA gene was driven either by the CaMV 35S/AMV or the E-8 fruit-ripening-specific promoter. Southern blot hybridization confirmed
the stable integration of the chimeric uidA gene into the tomato genome. Fruit and leaf tissues were collected from T0 and T1 plants, and assayed for β-glucuronidase (GUS) enzyme activity. As expected, both vegetative and fruit tissues of transgenic
plants carrying the uidA gene under the control of CaMV 35S/AMV showed varying levels of GUS activity, while no expression was observed in vegetative
tissues of transgenic plants carrying the uidA gene driven by the E-8 promoter. All fruits from transgenic plants produced with both sets of constructs displayed expression
of the uidA gene. However, when this reporter gene was driven by the CaMV 35S/AMV, GUS activity levels were significantly higher than
when it was driven by the E-8 fruit-specific promoter. The presence/absence of the uidA gene in T1 plants segregated in a 3∶1 Mendelian ratio. 相似文献
14.
Gittins John R. Hiles Elizabeth R. Pellny Till K. Biricolti Stefano James David J. 《Molecular breeding : new strategies in plant improvement》2001,7(1):51-62
The Brassica napus extensin A gene is highly expressed in root tissue of oilseed rape. In an attempt to identify an effective root-specific promoter for biotechnological applications, we have examined the ability of the –940 extA promoter to drive expression of the gusA reporter gene in the vegetative tissues of apple (Malus pumila Mill cv. Greensleeves). Transgenic apple lines were produced by Agrobacterium tumefaciens-mediated transformation and GUS activity was analysed both quantitatively and qualitatively. The extA promoter was active in all tissues of young plants in all 15 clones examined. However Southern blot data suggested that only a proportion of the population contained the entire promoter and that others had suffered deletions of unknown length. This may have contributed to the variation seen in the quantitative and qualitative expression of GUS. Specific GUS activity was highest in the stems where it approached, and in some clones, exceeded that using the constitutive CaMV 35S promoter. Histochemical analysis confirmed that GUS was localised to tissues involved in structural support of the stem. Staining was particularly intense at nodal junctions where high tensile stress is exerted on the tissues. Maturing phloem tissues showed localisation of expression to the phloem parenchyma cells and phloem fibres. Transverse sections of the root revealed staining of primary procambial tissues including the young endodermis but no staining was seen in the cortex. Although the –940 extA promoter is clearly not root-specific in apple, it is likely to have useful biotechnological applications in tree species. 相似文献
15.
Tal Mizrahi Joseph Heller Shoshana Goldenberg Zeev Arad 《Cell stress & chaperones》2010,15(4):351-363
Land snails are subject to daily and seasonal variations in temperature and in water availability and depend on a range of
behavioral and physiological adaptations for coping with problems of maintaining water, ionic, and thermal balance. Heat shock
proteins (HSPs) are a multigene family of proteins whose expression is induced by a variety of stress agents. We used experimental
desiccation to test whether adaptation to different habitats affects HSP expression in two closely related Sphincterochila snail species, a desiccation-resistant, desert species Sphincterochila zonata, and a Mediterranean-type, desiccation-sensitive species Sphincterochila cariosa. We examined the HSP response in the foot, hepatopancreas, and kidney tissues of snails exposed to normothermic desiccation.
Our findings show variations in the HSP response in both timing and magnitude between the two species. The levels of endogenous
Hsp72 in S. cariosa were higher in all the examined tissues, and the induction of Hsp72, Hsp74, and Hsp90 developed earlier than in S. zonata. In contrary, the induction of sHSPs (Hsp25 and Hsp30) was more pronounced in S. zonata compared to S. cariosa. Our results suggest that land snails use HSPs as part of their survival strategy during desiccation and as important components
of the aestivation mechanism in the transition from activity to dormancy. Our study underscores the distinct strategy of HSP
expression in response to desiccation, namely the delayed induction of Hsp70 and Hsp90 together with enhanced induction of
sHSPs in the desert-dwelling species, and suggests that evolution in harsh environments will result in selection for reduced
Hsp70 expression. 相似文献
16.
Sulphate can induce differential expression of thioglucoside glucohydrolases (myrosinases) 总被引:1,自引:0,他引:1
Thioglucoside glucohydrolase (EC 3.2.3.1; myrosinase) hydrolyses glucosinolates and thereby liberates glucose and sulphur
and nitrogen compounds. To examine the hypothesis that the myrosinase-glucosinolate system is influenced by environmental
factors, the effect of sulphate on the expression of myrosinases was examined. On examining different plant organs at various
stages, it was observed that sulphate induces a differential expression of myrosinase polypeptides in plants ofSinapis alba L. (white mustard). Specific myrosinase polypeptides, dependent on sulphate in the growth medium, were detected on immunoblots.
Without sulphate a maximum of three polypeptides was detected in buds, two in cotyledons and one in stems and roots. In plants
cultured on medium with sulphate up to four polypeptides could be observed in cotyledons, five polypeptides in buds, two in
stems and one in roots. Expression of myrosinases was, in general, high in plants cultured on a medium supplemented with sulphate.
In floweringS. alba plants, sulphate-starved plants showed a higher expression of myrosinase in cotyledons and stems compared to plants fed with
sulphate. Sulphate-fed plants had a high expression in inflorescences and roots. The organ- and time-specific induction of
the myrosinase expression is discussed in relation to sulphate metabolism and availability of sulphate under normal conditions
of cultivation and in relation to protection of Brassicaceae species. This is the first evidence for a specific induction
of individual myrosinase proteins. 相似文献
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Kazuko Yamaguchi-Shinozaki Kazuo Shinozaki 《Molecular & general genetics : MGG》1993,236(2-3):331-340
Summary We characterized the expression of genes that correspond to a cDNA clone, RD29, which is induced by desiccation, cold and high-salt conditions in Arabidopsis thaliana. Northern analysis of desiccation-induced expression revealed a two-step induction process. Early induction occurs within 20 min and secondary induction occurs 3 h after the start of desiccation. Exogenous abscisic acid (ABA) induces RD29 mRNA within 3 h. Two genes corresponding to RD29, rd29A and rd29B, are located in tandem in an 8 kb region of the Arabidopsis genome and encode hydrophilic proteins. Desiccation induces rd29A mRNA with two-step kinetics, while rd29B is induced only 3 h after the start of desiccation. The expression of both genes is stimulated about 3 h after application of ABA. It appears that rd29A has at least two cis-acting elements, one involved in the ABA-associated response to desiccation and the other induced by changes in osmotic potential. The -glucuronidase (GUS) reporter gene driven by the rd29A promoter was induced at significant levels by desiccation, cold, high-salt conditions and ABA in both transgenic Arabidopsis and tobacco. Histochemical analysis of GUS activity revealed that the rd29A promoter functions in almost all the organs and tissues of vegetative plants during water deficiency. 相似文献
20.
Vegetative plants of Sinapis alba L. grown under short days were induced to flower by exposure to one long day or continuous long days. Irrespective of the number of long days, the first flower primordia were initiated by the shoot apical meristem 60 h after the start of the inductive treatment. An indirect histoimmunofluorescence technique was used to search in the apical meristem for three antigenic proteins which had been previously detected by immunodiffusion tests in the whole apical bud (Pierard et al. (1977) Physiol. Plant. 41, 254–258). One protein called protein A, present in the vegetative meristem, increased in concentration during the first 48 h following the start of the inductive treatment. It stayed constant up to 96 h and disappeared completely at a later time. Two other proteins called B and C, absent in the vegetative meristem, appeared in the meristem of induced plants between 30 and 36 h after the start of the inductive treatment and progressively accumulated at later times up to 240 h. These proteins appeared 8 h before the irreversible commitment of the meristem to produce flower primordia (point of no return) was reached and 24 h before start of flower production. These observations support an interpretation of floral evocation as consisting, at least partially, of an early and qualitative change in gene expression.Abbreviations AVB
anti-vegetative-bud antiserum
- ARB
antireproductive-bud antiserum
- IgG
immunoglobulins G
- TRITC
tetramethylrhodamine isothiocyanate
- GAR IgG
goat antirabbit IgG
- S0 IgG
non-immune rabbit IgG 相似文献