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1.
QTLs for enzyme activities and soluble carbohydrates involved in starch accumulation during grain filling in maize 总被引:4,自引:0,他引:4
Thévenot C Simond-Côte E Reyss A Manicacci D Trouverie J Le Guilloux M Ginhoux V Sidicina F Prioul JL 《Journal of experimental botany》2005,56(413):945-958
ADPglucose, the essential substrate for starch synthesis, is synthesized in maize by a pathway involving at least invertases, sucrose synthase, and ADPglucose pyrophosphorylase, as shown by the starch-deficient mutants, mn1, sh1, and bt2 or sh2, respectively. To improve understanding of the relationship between early grain-filling traits and carbohydrate composition in mature grain, QTLs linked to soluble invertase, sucrose synthase, and ADPglucose pyrophosphorylase activities and to starch, sucrose, fructose, and glucose concentrations were investigated. In order to take into account the specific time-course of each enzyme activity during grain filling, sampling was carried out at three periods (15, 25, and 35 d after pollination) on 100 lines from a recombinant inbred family, grown in the field. The MQTL method associated with QTL interaction analysis revealed numerous QTLs for all traits, but only one QTL was consistently observed at the three sampling periods. Some chromosome zones were heavily labelled, forming clusters of QTLs. Numerous possible candidate genes of the starch synthetic pathway co-located with QTLs. Four QTLs were found close to the locus Sh1 (bin 9.01) coding for the sucrose synthase. In order to confirm the importance of this locus, the CAPS polymorphism of the Sh1 gene was analysed in 45 genetically unrelated maize lines from various geographical origins. The DNA polymorphism was significantly associated with phenotypic traits related to grain filling (starch and amylose content, grain matter, and ADPglucose pyrophosphorylase activity at 35 DAP). Thus, the Sh1 locus could provide a physiologically pertinent marker for maize selection. 相似文献
2.
《Plant Physiology and Biochemistry》2000,38(6):459-472
The loci explaining the variability of quantitative traits related to starch content and composition (amylose, amylopectin and water soluble fraction) were searched for in maize kernels. Multifactorial genetic methods were used to detect and locate QTLs (quantitative trait loci) on a genetic map consisting mainly of RFLP markers for genes with known function. The genetic material was recombinant inbred lines originating from parents differing in starch structure (dent vs. flint). Kernels were harvested from field grown plants for two successive years and under two pollination systems. Main effect and epistasis QTLs were detected using two methods, composite interval mapping (MQTL) and ANOVA. Despite large year-to-year differences, physiologically meaningful co-locations were observed between trait QTLs. Moreover, the number of expressed sequences on our map allowed the search for co-locations between QTLs and genes involved in carbohydrate metabolism. The main co-location was between an amylose QTL and Shrunken 2 (SH2) locus, on chromosome 3 (SH2 encoding for the large subunit of ADPglucose pyrophosphorylase). The importance of this locus as a candidate gene for a starch QTL is in agreement with previous studies based either on QTL co-locations or on revertant analysis. Other co-locations were observed between amylose and amylopectin QTLs and the two loci of IVR1 invertase genes on chromosomes 2 and 10. Further comparison with previously detected QTLs for carbohydrate metabolism in maize leaves showed consistent co-location in map regions devoid of candidate genes, such as near chromosome 1S telomere. The possible contribution of regulatory genes in this region is discussed. 相似文献
3.
S. Pelleschi A. Leonardi J. -P. Rocher G. Cornic D. de Vienne C. Thévenot J. -L. Prioul 《Molecular breeding : new strategies in plant improvement》2006,17(1):21-39
One hundred to 120 maize recombinant inbred lines at the mature fourth leaf stage derived from F-2 and Io parental lines were
grown in a glasshouse and were deprived of water for 9 days in order to detect pertinent markers of the physiological response
to water stress which may be used for breeding. Carbohydrate metabolism QTLs were compared to photosynthesis gas exchange
QTLs. The locations of these QTLs were further compared with those of morphological trait QTLs when water availability varied.
The traits ranged from three enzyme activities (invertase, sucrose-P synthase, ADP glucose pyrophosphorylase) and hexose,
sucrose, starch content to CO2 uptake and stomatal conductance, water status, leaf size, root/shoot ratio, and ABA (leaf, root and xylem sap). Four main
results were obtained (1) only 14 % of QTLs were common to both drought and watered treatments, confirming the existence of
stress specific chromosome regions, (2) the QTLs tended to form clusters, frequently consisting of QTLs from different classes
(growth, photosynthesis, water status, carbohydrate metabolism and ABA), (3) carbohydrate metabolism trait QTLs were more
frequently co-located with growth trait QTLs than photosynthesis related ones, especially in control conditions, (4) one co-location
was observed between the three enzyme activities implied in sucrose and starch metabolism and a corresponding structural gene,
which can be considered as a candidate gene for explaining part of the variability of each enzymatic trait (invertase, sucrose-P
synthase, ADPglucose pyrophosphorylase). It is concluded that, carbohydrate metabolism provides valuable traits for understanding
and improving maize responses to water stress. 相似文献
4.
G. Srinivas K. Satish R. Madhusudhana R. Nagaraja Reddy S. Murali Mohan N. Seetharama 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(8):1439-1454
The identification of quantitative trait loci (QTLs) affecting agronomically important traits enable to understand their underlying
genetic mechanisms and genetic basis of their complex interactions. The aim of the present study was to detect QTLs for 12
agronomic traits related to staygreen, plant early development, grain yield and its components, and some growth characters
by analyzing replicated phenotypic datasets from three crop seasons, using the population of 168 F7 RILs of the cross 296B × IS18551. In addition, we report mapping of a subset of genic-microsatellite markers. A linkage map
was constructed with 152 marker loci comprising 149 microsatellites (100 genomic- and 49 genic-microsatellites) and three
morphological markers. QTL analysis was performed by using MQM approach. Forty-nine QTLs were detected, across environments
or in individual environments, with 1–9 QTLs for each trait. Individual QTL accounted for 5.2–50.4% of phenotypic variance.
Several genomic regions affected multiple traits, suggesting the phenomenon of pleiotropy or tight linkage. Stable QTLs were
identified for studied traits across different environments, and genetic backgrounds by comparing the QTLs in the study with
previously reported QTLs in sorghum. Of the 49 mapped genic-markers, 18 were detected associating either closely or exactly
as the QTL positions of agronomic traits. EST marker Dsenhsbm19, coding for a key regulator (EIL-1) of ethylene biosynthesis,
was identified co-located with the QTLs for plant early development and staygreen trait, a probable candidate gene for these
traits. Similarly, such exact co-locations between EST markers and QTLs were observed in four other instances. Collectively,
the QTLs/markers identified in the study are likely candidates for improving the sorghum performance through MAS and map-based
gene isolations. 相似文献
5.
Ivr2, a candidate gene for a QTL of vacuolar invertase activity in maize leaves. Gene-specific expression under water stress 总被引:7,自引:0,他引:7
Pelleschi S Guy S Kim JY Pointe C Mahé A Barthes L Leonardi A Prioul JL 《Plant molecular biology》1999,39(2):373-380
Water shortage produced an early and large stimulation of acid- soluble invertase activity in adult maize leaves whereas cell wall invertase activity remained constant. This response was closely related to the mRNA level for only one of the invertase gene (Ivr2), encoding a vacuolar isoform. In parallel, four quantitative trait loci (QTLs) were detected for invertase activity under control and nine under stressful conditions. One QTL in control and one in stressed plants was located near to the lvr2 gene on chromosome 5. Other QTLs for invertase activity were found close to carbohydrate QTLs; some of them formed stress clusters. 相似文献
6.
Zygier S Chaim AB Efrati A Kaluzky G Borovsky Y Paran I 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(3):437-445
Quantitative trait locus (QTL) mapping for fruit weight and shape in pepper (Capsicum spp.) was performed using C. chinense and C. frutescens introgression lines of chromosomes 2 and 4. In chromosome 2, a single major fruit-weight QTL, fw2.1, was detected in both populations that explained 62% of the trait variation. This QTL, as well as a fruit-shape QTL, fs2.1, which had a more minor effect, were localized to the tomato fruit-shape gene ovate. The cloned tomato fruit-weight QTL, fw2.2, did not play a major role in controlling fruit size variations in pepper. In chromosome 4, two fruit-weight QTLs, fw4.1 and fw4.2, were detected in the same genomic regions in both mapping populations. In addition, a single fruit-shape QTL was detected in each of the mapping populations that co-localized with one of the fruit-weight QTLs, suggesting pleiotropy or close linkage of the genes controlling size and shape. fw2.1 and fw4.2 represent major fruit-weight QTLs that are conserved in the three Capsicum species analyzed to date for fruit-size variations. Co-localization of the pepper QTLs with QTLs identified for similar traits in tomato suggests that the pepper and tomato QTLs are orthologous. Compared to fruit-shape QTLs, fruit-weight QTLs were more often conserved between pepper and tomato. This implies that different modes of selection were employed for these traits during domestication of the two Solanaceae species.S. Zygier and A. Ben Chaim contributed equally to this work. 相似文献
7.
Starch Synthesis in Developing Potato Tubers 总被引:3,自引:0,他引:3
The activities of enzymes involved in starch metabolism were measured at intervals during tuberization and the early stages of tuber growth in Solanum tubersum grown in water culture under controlled environmental conditions. Starch synthase, ADPglucose pyrophosphorylase, UDPglucose pyrophosphorylase and phosphorylase activities all increased during tuber development, the most pronounced increases occurring in the activities of ADP-glucose pyrophosphorylase and phosphorylase. The activity ratio ADPglucose pyrophosphorylase/phosphorylase was lowest in slow growing tubers and hightest in fast growing tubers. In addition, high sugar concentrations in fast growing tubers and low sugar concentrations in slow growing tubers suggested that enzyme levels might be influenced by sugar concentration. The activities of starch synthase, phosphorylase and ADPglucose pyrophosphorylase were increased 2–2.5 fold by the presence of 100 mM K+. It is concluded that the major enzyme changes occur as a consequence of tuber initiation and that starch accumulation is controlled, at least in part, by the activities of ADPglucose pyrophosphorylase and phosphorylase. 相似文献
8.
M Obara M Kajiura Y Fukuta M Yano M Hayashi T Yamaya T Sato 《Journal of experimental botany》2001,52(359):1209-1217
Ninety-eight backcross inbred lines (BC1F6) developed between Nipponbare, a japonica rice, and Kasalath, an indica rice were employed to detect putative quantitative trait loci (QTLs) associated with the contents of cytosolic glutamine synthetase (GS1; EC 6.3.1.2) and NADH-glutamate synthase (NADH-GOGAT; EC 1.4.1.14) in leaves. Immunoblotting analyses showed transgressive segregations toward lower or greater contents of these enzyme proteins in these backcross inbred lines. Seven chromosomal QTL regions for GS1 protein content and six for NADH-GOGAT protein content were detected. Some of these QTLs were located in QTL regions for various biochemical and physiological traits affected by nitrogen recycling. These findings suggested that the variation in GS1 and NADH-GOGAT protein contents in this population is related to the changes in the rate of nitrogen recycling from senescing organs to developing organs, leading to changes in these physiological traits. Furthermore, a structural gene for GS1 was mapped between two RFLP markers, C560 and C1408, on chromosome 2 and co-located in the QTL region for one-spikelet weight. A QTL region for NADH-GOGAT protein content was detected at the position mapped for the NADH-GOGAT structural gene on chromosome 1. A QTL region for soluble protein content in developing leaves was also detected in this region. Although fine mapping is required to identify individual genes in the future, QTL analysis could be a useful post-genomic tool to study the gene functions for regulation of nitrogen recycling in rice. 相似文献
9.
A. Börner V. Korzun A. V. Voylokov W. E. Weber 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(6-7):1087-1090
Progenies of an F2 mapping population were analyzed for quantitative traits to detect QTLs by using marker information from F2 plants for chromosome 5R. The mapping population was segregating for the major dwarfing gene Ddw1 and the gene Hp1 for hairy peduncle. The only QTL determining plant height was located between HP1 and Ddw1 on the distal part of chromosome 5RL. At the same position a QTL for peduncle length was found, and this trait was closely
related to plant height (r=0.895). Since Hp1 and Ddw1 are dominant marker loci, no dominance effect could be estimated. The QTLs for spike length and the number of florets were
located near the centromere on 5RL. These two traits were correlated with r=0.824 and showed partial dominance, but these traits were not correlated to plant height and peduncle length. Homoeologous
relationships between the QTLs mapped for the first time in rye and those mapped in other Triticeae members are discussed.
Received: 8 June 1998 / Accepted: 8 October 1998 相似文献
10.
Quilot B Wu BH Kervella J Génard M Foulongne M Moreau K 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,109(4):884-897
Genetic control of the different attributes involved in peach quality has been investigated in an advanced backcross population derived from a cross between Prunus davidiana clone P1908, a wild parent with poor agronomic performance, and a commercial variety, Summergrand. A total of 24 physical and biochemical traits were investigated. Quantitative trait loci (QTLs) were detected for all the traits studied. We identified alleles from P. davidiana with agronomically favorable effects regarding fruit and stone sizes, sugar and acid concentrations and red flesh coloration, in clear contrast to its phenotype. We identified three main regions of the genome where alleles from P. davidiana had negative effects on multiple traits. In other regions, co-locations of QTLs with opposite effects on quality traits were also detected. We discuss the nature of these co-locations in the light of the probable physiological mechanisms involved. Strategies to cope with negative correlations between favorable traits and co-locations of P. davidiana alleles with negative effects on quality traits and positive effects regarding resistance to powdery mildew are discussed from a breeding point of view. 相似文献
11.
Bala R. Thumma Simon G. Southerton John C. Bell John V. Owen Martin L. Henery Gavin F. Moran 《Tree Genetics & Genomes》2010,6(2):305-317
To identify the chromosomal regions affecting wood quality traits, we conducted a genome-wide quantitative trait locus (QTL)
analysis of wood quality traits in Eucalyptus nitens. This information is important to exploit the full potential of the impending Eucalyptus genome sequence. A three generational mapping population consisting of 296 progeny trees was used to identify QTL associated
with several wood quality traits in E. nitens. Thirty-six QTL positions for cellulose content, pulp yield, lignin content, density, and microfibril angle (MFA) were identified
across different linkage groups. On linkage groups (LG)2 and 8, cellulose QTL cluster with pulp yield and extractives QTL
while on LG4 and 10 cellulose and pulp yield QTLs cluster together. Similarly, on LG4, 5, and 6 QTL for lignin traits were
clustered together. At two positions, QTL for MFA, a physical trait related to wood stiffness, were clustered with QTL for
lignin traits. Several cell wall candidate genes were co-located to QTL positions affecting different traits. Comparative
QTL analysis with Eucalyptus globulus revealed two common QTL regions for cellulose and pulp yield. The QTL positions identified in this study provide a resource
for identifying wood quality genes using the impending Eucalyptus genome sequence. Candidate genes identified in this study through co-location to QTL regions may be useful in association
studies. 相似文献
12.
Yujia Leng Shuilian Wang Ruoan Wang Tao Tao Shuwen Jia Tao Song Lina Xu Xiuling Cai Sukui Jin Jiping Gao 《Phyton》2022,91(5):943-958
Grain size traits are critical agronomic traits which directly determine grain yield, but the genetic bases of these traits are still not well understood. In this study, a total of 154 chromosome segment substitution lines (CSSLs) population derived from a cross between a japonica variety Koshihikari and an indica variety Nona Bokra was used to investigate grain length (GL), grain width (GW), length-width ratio (LWR), grain perimeter (GP), grain area (GA), and thousand grain weight (TGW) under four environments. QTL mapping analysis of six grain size traits was performed by QTL IciMapping 4.2 with an inclusive composite interval mapping (ICIM) model. A total of 64 QTLs were identified for these traits, which mapped to chromosomes 1, 2, 3, 4, 6, 7, 8, 10, 11, and 12 and accounted for 1.6%–27.1% of the total phenotypic variations. Among these QTLs, thirty-six loci were novel and seven QTLs were identified under four environments. One locus containing the known grain size gene, qGL3/GL3.1/OsPPKL1, also have been found. Moreover, five pairs of digenic epistatic interactions were identified except for GL and GP. These findings will facilitate fine mapping of the candidate gene and QTL pyramiding to genetically improve grain yield in rice. 相似文献
13.
Julier B Huguet T Chardon F Ayadi R Pierre JB Prosperi JM Barre P Huyghe C 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,114(8):1391-1406
In many legume crops, especially in forage legumes, aerial morphogenesis defined as growth and development of plant organs,
is an essential trait as it determines plant and seed biomass as well as forage quality (protein concentration, dry matter
digestibility). Medicago truncatula is a model species for legume crops. A set of 29 accessions of M. truncatula was evaluated for aerial morphogenetic traits. A recombinant inbred lines (RILs) mapping population was used for analysing
quantitative variation in aerial morphogenetic traits and QTL detection. Genes described to be involved in aerial morphogenetic
traits in other species were mapped to analyse co-location between QTLs and genes. A large variation was found for flowering
date, morphology and dynamics of branch elongation among the 29 accessions and within the RILs population. Flowering date
was negatively correlated to main stem and branch length. QTLs were detected for all traits, and each QTL explained from 5.2
to 59.2% of the phenotypic variation. A QTL explaining a large part of genetic variation for flowering date and branch growth
was found on chromosome 7. The other chromosomes were also involved in the variation detected in several traits. Mapping of
candidate genes indicates a co-location between a homologue of Constans gene or a flowering locus T (FT) gene and the QTL
of flowering date on chromosome 7. Other candidate genes for several QTLs are described.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
14.
Linkage Disequilibrium Mapping of Morphological, Resistance, and Other Agronomically Relevant Traits in Modern Spring Barley Cultivars 总被引:6,自引:0,他引:6
A. T. W. Kraakman F. Martínez B. Mussiraliev F. A. van Eeuwijk R. E. Niks 《Molecular breeding : new strategies in plant improvement》2006,17(1):41-58
A set of 148 modern spring barley cultivars was explored for the extent of linkage disequilibrium (LD) between genes governing
traits and nearby marker alleles. Associations of agronomically relevant traits (days to heading, plant height), resistance
traits (leaf rust, barley yellow dwarf virus (BYD)), and morphological traits (rachilla hair length, lodicule size) with AFLP
markers and SSR markers were found. Known major genes and QTLs were confirmed, but also new putative QTLs were found. The
LD mapping clearly indicated the common occurrence of Rph3, a gene for hypersensitivity resistance against Puccinia hordei, and also confirmed the QTL Rphq2 for prolonging latency period of P. hordei in seedlings. We also found strong indication for a hitherto not reported gene for resistance or tolerance to BYD on chromosome
2, linked to SSR marker HVM054. Our conclusion is that LD mapping is a valuable additional tool in the search for applicable
marker associations with major genes and QTLs.
Electronic supplementary material Electronic supplementary material is available for this article at
and accessible for authorised users. 相似文献
15.
Location of genes involved in ear compactness in wheat (Triticum aestivum) by means of molecular markers 总被引:4,自引:0,他引:4
Sourdille P. Tixier M.H. Charmet G. Gay G. Cadalen T. Bernard S. Bernard M. 《Molecular breeding : new strategies in plant improvement》2000,6(3):247-255
Quantitative trait loci (QTLs) for three traits related to ear morphology (spike length, number of spikelets, and compactness as the ratio between number of spikelets and spike length) in wheat (Triticum aestivum L.) were mapped in a doubled-haploid (DH) population derived from the cross between the cultivars Courtot and Chinese Spring. A molecular marker linkage map of this cross that had previously been constructed based on 187 DH lines and 380 markers was used for QTL mapping. The genome was well covered (85%) except chromosomes 1D and 4D and a set of anchor loci regularly spaced (one marker each 15.5 cM) were chosen for marker regression analysis. The presence of a QTL was declared at a significance threshold = 0.001. The population was grown in one location under field conditions during three years (1994, 1995 and 1998). For each trait, 4 to 6 QTLs were identified with individual effects ranging between 6.9% and 21.8% of total phenotypic variation. Several QTLs were detected that affected more than one trait. Of the QTLs 50% were detected in more than one year and two of them (number of spikelets on chromosome 2B, and compactness on chromosome 2D) emerged from the data from the three years. Only one QTL co-segregated with the gene Q known to be involved in ear morphology, namely the speltoid phenotype. However, this chromosome region explained only a minor part of the variation (7.5–11%). Other regions had a stronger effect, especially two previously unidentified regions located on chromosomes 1A and 2B. The region on the long arm of chromosome 1A was close to the locus XksuG34-1A and explained 12% of variation in spike length and 10% for compactness. On chromosome 2B, the QTL was detected for the three traits near the locus Xfbb121-2B. This QTL explained 9% to 22% of variation for the traits and was located in the same region as the gene involved in photoperiod response (Ppd2). Other regions were located at homoeologous positions on chromosomes 2A and 2D. 相似文献
16.
Dunwei Ci Dong Jiang Sishen Li Bernd Wollenweber Tingbo Dai Weixing Cao 《Acta Physiologiae Plantarum》2012,34(1):191-202
Quantitative trait loci (QTL) for Cadmium (Cd) tolerance and accumulation in wheat (Triticum aestivum L.) were identified, using 103 recombinant inbred lines (RILs) derived from a cross of Ch × Sh at germination and seedling
stages. The traits of germination, growth and physiology were measured. Cd tolerance indexes (TI) were calculated for plants
under Cd stress relative to control conditions. Cd concentrations in both root and shoot were determined and the amount of
Cd accumulation and translocation calculated. The phenotypic variation of the above traits showed a continuous distribution
pattern among the RILs. Twenty-six QTLs were detected, (16 of which were designated for the traits under the control and Cd
stress, 8 for Cd tolerance and 2 for root Cd accumulation). These 26 QTLs individually could explain 7.97–60.16% of the phenotypic
variation. Fourteen QTLs were positive (with the additive effects coming from Ch) while the remaining 12 QTLs were negative
(with the additive effects contributed by Sh). No QTL were detected in the same region on the chromosomes of wheat. The results
indicated that genetic mechanisms controlling the traits of Cd tolerance were independent from each other. Therefore, in this
study, the properties of Cd tolerance and accumulation showed to be independent traits in wheat. 相似文献
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Kunert A Naz AA Dedeck O Pillen K Léon J 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,115(5):683-695
The advanced backcross QTL (AB-QTL) strategy was utilised to locate quantitative trait loci (QTLs) for baking quality traits
in two BC2F3 populations of winter wheat. The backcrosses are derived from two German winter wheat cultivars, Batis and Zentos, and two
synthetic, hexaploid wheat accessions, Syn022 and Syn086. The synthetics originate from hybridisations of wild emmer (T. turgidum spp. dicoccoides) and T. tauschii, rather than from durum wheat and T. tauschii and thus allowed for the first time to test for exotic QTL effects on wheat genomes A and B in addition to genome D. The
investigated quality traits comprised hectolitre weight, grain hardness, flour yield Type 550, falling number, grain protein
content, sedimentation volume and baking volume. One hundred and forty-nine SSR markers were applied to genotype a total of
400 BC2F3 lines. For QTL detection, a mixed-model ANOVA was conducted, including the effects DNA marker, BC2F3 line, environment and marker × environment interaction. Overall 38 QTLs significant for a marker main effect were detected.
The exotic allele improved trait performance at 14 QTLs (36.8%), while the elite genotype contributed the favourable effect
at 24 QTLs (63.2%). The favourable exotic alleles were mainly associated with grain protein content, though the greatest improvement
of trait performance due to the exotic alleles was achieved for the traits falling number and sedimentation volume. At the
QTL on chromosome 4B the exotic allele increased the falling number by 19.6% and at the QTL on chromosome 6D the exotic allele
led to an increase of the sedimentation volume by 21.7%. The results indicate that synthetic wheat derived from wild emmer × T. tauschii carries favourable QTL alleles for baking quality traits, which might be useful for breeding improved wheat varieties by
marker-assisted selection. 相似文献