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1.
Sancak Cengiz Mirici Semra Özcan Sebahattin 《Plant Cell, Tissue and Organ Culture》2000,61(3):231-235
An in vitro propagation protocol has been developed from mature trees of Pittosporum napaulensis. The best bud proliferation (83.1%), shoot number (21 axillary shoots/ explant) and shoot length (5.5 cm) was achieved in
Murashige and Skoog (MS) medium supplemented with 5.0 μM N−6 benzyladenine and 0.1 μM α- naphthalene acetic acid. Of the three cytokinins tested (N−6 benzyladenine, kinetin and thidiazuron), N−6 benzyladenine proved to be the best for shoot induction. Shoot regeneration potential varied among genotypes. Regenerated
shoots rooted after 48 hours treatment on half-strength MS liquid medium supplemented with 20 μM indole-3-butyric acid. Rooted
shoots transferred to 120 g (w/v) soilrite + sand + soil (1:1:1) mixture showed 70% survival. Twenty-one plantlets are growing
well in green house conditions.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
2.
Callus cultures were obrained from petiole explants of Carica papaya on MS medium containing 0.5–10.5 μM α-naphthaleneacetic acid (NAA) in combination with 0.5–5 μM benzyladenine (BA). Hard-green calli were transferred to MS medium
containing 100 mgl−1 casein hydrolysate (CH) with specific BA-NAA formulation, where they developed adventitious buds within 2 weeks of culture.
Maximum number of adventitious buds were obtained in 2 μM BA and 0.1 μM NAA. Shoot regeneration occurred from these adventitious
buds by the end of the 4th week. Regenerated shoots were elongated in hormone-free medium and rooted in half-strength MS fortified
with 3 UM NAA and 0.5 μM gibberellic acid (GA3). The regenerants were transferred to soil after acclimatization. 相似文献
3.
A rapid and efficient micropropagation system was developed for Psoralea corylifolia, an endangered, valuable medicinal plant. Multiple shoot buds were obtained in half-strength liquid Phillips–Collins (L2)
medium supplemented with 5 μM benzylaminopurine (BA) and 5 μM thidiazuron (TDZ) from apical bud explants of 1-week-old cultures.
The shoot buds were subcultured on enriched solid L2 medium supplemented with different concentrations and combinations of
BA, kinetin (KIN), 2-isopentenyladenine (2iP), TDZ, bavistin (BVN) and trimethoprim (TMP). Enriched solid L2 medium supplemented
with 2 μM BA, 1 μM TDZ and 100 mg l−1 BVN were more effective in producing greater number of shoots per explant (85.2 ± 0.9 shoots/explant) after 4 weeks of culture.
The regenerated shoots (40–50 mm in length) rooted and accompanied by hardening upon transfer to 50 μM indole-3-butyric acid
(IBA) for 15 min and followed by planting in sterile soil mixture and vermiculate (3:1 v/v), with 50 ml of one-eight strength
L2 basal salt solution devoid of sucrose and inositol, supplemented with 5 μM IBA and 100 mg l−1 BVN. The plants achieved 100% rooting with hardening. Subsequently the rooted plants were successfully established in the
field. The survival percentage differed with seasonal variations. The concentration of psoralen was evaluated in different
tissues of ex vitro and in vivo grown plants by high-performance liquid chromatography (HPLC). Psoralen content was increased
in leaves (2.97%), roots (2.38%), stems (5.40%) and seeds (1.63%) of ex vitro plants than the in vivo plants. This system
facilitates for commercial and rapid propagation of P. corylifolia for conservation strategies and phytomedicine production. 相似文献
4.
Quoirin Marguerite da Silva Mônica C. Martins Kelly G. de Oliveira Dulce E. 《Plant Cell, Tissue and Organ Culture》2001,66(3):199-205
The influence of mineral formulation, growth regulators and activated charcoal on micropropagation of juvenile black wattle
(Acacia mearnsii) was studied. Nodal segments of one-month-old seedlings were used as starting material. After 30 to 45 days, they developed
into shoots, which were divided into microcuttings and transferred to fresh media. The addition of 2 g l−1 of activated charcoal to basal medium (3/4 strength MS salts and MS vitamins) improved the multiplication phase, reducing
leaf chlorosis and raising the percentage of elongated shoots. The multiplication rate varied between 1.7 and 3 every month.
Rooting percentage too was higher in the presence of charcoal, even when auxin was not added to the culture medium. The addition
of 8.87 μM of benzyladenine and/or 1.44 or 2.88 μM of gibberellic acid did not influence significantly the multiplication,
nor modifications in the FeSO4, MnSO4, CaCl2 content of the medium. The system described allows the multiplication, elongation and rooting of black wattle in one step.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
5.
Manickam V.S. Elango Mathavan R. Antonisamy R. 《Plant Cell, Tissue and Organ Culture》2000,62(3):181-185
The development of stem callus mediated plant regeneration system for Withania somnifera is described. Maximum callus proliferation was obtained on Murashige and Skoog medium supplemented with 2.26 μM 2,4-D. Three-week-old,
white, friable callus was used for shoot regeneration. The maximum shoot regeneration (6.2 ± 0.34 shoots/explant) was achieved
in four weeks when callus was cultured on MS medium fortified with 4.44 μM BA and 0.57 μM IAA. Regenerated shoots were excised
and multiplied (8.4 ± 0.43 shoots/explant) on MS medium supplemented with 4.44 μM of BA. Multiple shoots were divided into
single shoots and were rooted (5.1 ± 0.49 rootlets/shoot) on half strength MS medium supplemented with 9.84 μM of IBA. After
a hardening phase of 3 weeks the plantlets were transferred to the field.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
6.
Tiwari Vaibhav Tiwari Kavindra Nath Singh Brahma Deo 《Plant Cell, Tissue and Organ Culture》2001,66(1):9-16
A mass in vitro propagation system for Bacopa monniera (L.) Wettst. (Scrophulariaceae), a medicinally important plant, has been developed. A range of cytokinins have been investigated
for multiple shoot induction with node, internode and leaf explants. Of the four cytokinins (6-benzyladenine, thidiazuron,
kinetin and 2-isopentenyladenine) tested thidiazuron (6.8 μM) and 6-benzyladenine (8.9 μM) proved superior to other treatments. Optimum adventitious shoot buds induction occurred at 6.8 μM thidiazuron where an average of 93 shoot buds were produced in leaf explants after 7 weeks of incubation. However, subculture
of leaf explants on medium containing 2.2 μM benzyladenine yielded a higher number (129.1) of adventitious shoot buds by the end of third subculture. The percentage
shoot multiplication (100%) as well as the number of shoots per explant remained the high during the first 3 subculture cycles,
facilitating their simultaneous harvest for rooting. In vitro derived shoots were elongated on growth regulator-free MS medium and exhibited better rooting response on medium containing
4.9 μM IBA. After a hardening phase of 3 weeks, there was an almost 100% transplantation success in the field.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
7.
Summary Axillary and terminal buds from suckers of Ananas comosus cv. Phuket were established on Murashige and Tucker-based (MT) medium with 2.0 mgl−1 (9.8 μM) indolebutyric acid, 2.0 mgl−1 (10.74 μM) naphthaleneacetic acid, and 2.0 mgl−1 (9.29 μM) kinetin, followed by multiplication on Murashige and Skoog-based (MS) medium containing 2.0 mgl−1 (8.87 μM) benzyladenine (BA) to provide a continuous supply of axenic shoots. Leaves, excised from such cultured shoots, produced
adventitious shoots from their bases when these explants were cultured on MS medium containing 0.5 mgl−1 (2.26 μM) 2,4-dichlorophenoxyacetic acid (2,4-D) and 2.0 mgl−1 (8.87 μM) BA. Embryogenic callus was produced when leaf explants were cultured on MS medium with 3.0 mgl−1 (12.42 μM) 4-amino-3,5,6-trichloropicolinic acid (picloram). Somatic embryos developed into shoots following transfer of embryogenic
tissues to MS medium with 1.0 mgl−1 (4.44 μM) BA. Cell suspensions, initiated by transfer of embryogenic callus to liquid MS medium with 1.0 mgl−1 (4.14 μM) picloram or 1.0 mgl−1 (4.52 μM) 2,4-D, also regenerated shoots by somatic embryogenesis, on transfer of cells to semisolid MS medium with 1.0 mgl−1 (4.44 μM) BA. All regenerated shoots rooted on growth regulator-free MS medium, prior to ex vitro acclimation and transfer to the glasshouse. These studies provide a baseline for propagation, conservation, and genetic manipulation
of elite pineapple germplasms. 相似文献
8.
A simple, rapid and efficient protocol for micropropagation of Cardiospermum halicacabum via axillary bud multiplication has been successfully developed. The organogenic competence of nodal segments was investigated
on Murashige and Skoog (MS) medium supplemented with different concentrations of benzyladenine (BA), kinetin (Kn), thidiazuron
(TDZ) and 2-isopentenyladenine (2-iP). Multiple shoots differentiated directly without callus mediation within 4 weeks when
explants were cultured on a medium fortified with cytokinins. The maximum number of shoots (14.83 ± 0.52) was developed on
a medium supplemented with 0.3 μM TDZ. Such proliferating shoots when subcultured onto MS media devoid of TDZ gave the highest
rate of shoot multiplication (35.66 ± 1.00) by the end of fourth subculture passage. Elongated shoots were rooted on 1/3 MS
medium augmented with 0.5 μM IAA. The plantlets thus obtained were successfully hardened and transferred to greenhouse. 相似文献
9.
Summary Callus cultures were initiated from in vitro grown leaf, stem and root segments of Lonicera japonica “Hall's Prolific”, on a medium containing 10.7 μM α-naphthtylacetic acid and 2.7 μM benzyladenine, while media with 2,4-dichlorophenoxyacetic acid led to a rapid necrosis of explants. Shoot regeneration from
true-callus (i.e. without any part of the original explant) was achieved for the three different source tissues within 12 weeks. The highest
rate of regeneration was obtained by using benzyladenine (4.4 to 44.4 μM) as the sole hormone in the medium. The regenerated shoots were readily elongated and rooted on the same medium as used
for multiplication, and plantlets were subsequently transferred to greenhouse conditions, where nearly 100% of them were successfully
acclimatized. This is the first example of plant regeneration from aged (≥6 month-old) true-callus of a woody ornamental species. 相似文献
10.
Internode explants collected from in vitro grown shoots of two clones of Fagus sylvatica L. (European beech) and five clones of F. orientalis Lipski (Oriental beech) were used to evaluate their bud regeneration capacity. Adventitious shoot-buds formed on callus,
which developed from internode segments cultured in a Woody Plant Medium supplemented with different concentrations of either
thidiazuron (TDZ) or benzyladenine (BA). After 4 weeks of culture on induction media, the explants were transferred to a proliferation
medium supplemented with 2.2 μM BA, 9.1 μM zeatin and 2.9 μM indole-3-acetic acid (IAA) for another 8 weeks. Medium containing
TDZ was much more efficient than medium containing BA in inducing adventitious buds, the optimal TDZ concentration being 4.5
μM and the optimal BA concentration 17.8 μM. Genotypic variation in shoot regeneration capacity was observed among the two
Fagus species and between clones within each species, with a significant interaction between TDZ concentration and genotype regarding
mean bud number. Thidiazuron induction medium supplemented with a range of individual auxins was investigated, and it was
found that IAA or indole-3-butyric acid at 2.9 μM enhanced the bud forming capacity of explants. Morphogenic response varied
significantly with the position of the internode along the stem. The highest regeneration potential was obtained from apical
internodes, while those distal to the apex were the least productive. Elongated shoots of adventitious origin can be readily
proliferated by axillary branching.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
11.
Caulogenic responses of various explant types from 12-month-old plants of Hemidesmus indicus were tested. Second and third visible nodes (0.5 cm) from the apex and root segments (0.5 cm) were the most and least regenerative
respectively, with the formation of 9.37 and 2.6 shoots in 4 weeks on half strength MS medium supplemented with 2.22 μM BA
and 1.07 μM NAA and 4.44 μM BA and 2.69 μM NAA respectively. Caulogenic ability of the nodes decreased with increasing maturity.
Shoot buds initiated upon the young nodes on day 10 developed into 7.2 cm long shoots within 4 weeks thereby making a shoot
elongation phase unnecessary. Nodal explants of the in vitro raised shoots subcultured in the same medium produced 9.32 shoots of 7.1 cm length in 3–4 weeks, similar to those of the
mature plant-derived nodes. Multiplication through subculture of the nodes up to 25 passages of 4 weeks each was achieved
without decline. Shoot cultures were rooted in quarter salt strength MS medium containing 9.8 μM IBA and the rooted plants
were hardened for establishment in pots at 96% rate. Four months after establishment, the micropropagated plants were stable
and showed uniform morphological and growth characteristic. After 12 months of cultivation in the field, on an average micropropagated
plant consisted of 4–5 shoots, 5–8 branches per shoot and increased root biomass (13.5 g) compared to the poor growth (single
shoot and 2–3 branches) and root production (4.6 g) values obtained with plants raised from conventional rooted stem cuttings.
The concentration of the root specific compound, 2-hydroxy 4-methoxy benzaldehyde per plant was 2–3 fold higher in micropropagated
plants though on unit dry root biomass (0.12% per g dry wt) basis it remained the same between two sources of plants.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
12.
In vitro propagation of a semi-dwarfing cherry rootstock 总被引:2,自引:0,他引:2
Muna AL-Sabbagh Ahmad Abdul-Kader Mahmoud Khoder Abdul-Rahman Kalhout 《Plant Cell, Tissue and Organ Culture》1999,59(3):203-208
A successful in vitro propagation system for the semi-dwarfing cherry rootstock Maxma-14 (Prunus avium L.) has been developed. Shoot tips and axillary buds were successfully established in vitro. Multiplication rate of about 6 was achieved over a 4-week period using Murashige and Skoog medium with 4.44 μM benzyladenine
and 0.49 μM indole-3-butyric acid (IBA). Rooting occurred within 4 weeks on liquid and agar-gelled media containing 0.49 μM
NAA or 0.49, 2.45 μM IBA. On liquid media, a maximum rooting efficiency of up to 100% was obtained. However, high concentrations
of auxins delayed the time of root initiation for 3–5 days. Acclimatization was affected directly by rooting conditions. Survival
was best when plantlets were transferred to pots after a short period of root emergence on rooting media. Multiplication medium
was also important for successful acclimatization. Shoots transferred to rooting media from that with kinetin resulted in
better acclimatization and survival than that derived from media with benzyladenine. Further, plantlets rooted on liquid media
had better survival than that rooted on agar-gelled media.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
13.
Summary A micropropagation procedure for the adult cherimoya tree (Annona cherimola Mill.) is described. Axillary shoot proliferation was obtained after culturing nodal sections from Annona cherimola cv. ‘Fino de Jete’, on Murashige and Skoog (MS) medium supplemented with 2.28 μM zeatin. Roots were induced after preincubation of shoots for 3d in light on MS basal medium supplemented with lgl−1 activated charcoal, followed by culturing for 10 d (7 d dark and 3 d light) on MS medium with 492 μM indole-3-butyrie acid (IBA), 15 gl−1 sucrose, and 200 mgl−1 citric acid. Sixty-eight percent of induced shoots rooted after transferring to the same medium without auxin and with the
macroelements at half strength and the sucrose at 20gl−1. About 65% of rooted shoots survived after acclimatization. The procedures described herein may prove useful for clonal micropropagation
of selected genotypes of cherimoya. 相似文献
14.
Shoot regeneration was achieved from in vitro-produced leaves of Elaeagnus angustifolia L. Half-leaf explants from the terminal part of the shoot produced more shoots than explants from the basal part of the in vitro-derived shoots on agar-solidified WPM medium supplemented with 1 M benzyladenine (BA). In liquid medium of the same formulation, compact shoots that did not elongate were formed on the explants. Leaf cross-section explants (1 mm thick) produced shoots both on solid and liquid medium with 1 M BA, whereas again compact shoots were formed with 10 M BA. Further shoot development on these explants was promoted by their transfer to fresh solid medium containing 1 M BA and 1 M gibberellic acid (GA3).Abbreviations BA
benzyladenine
- GA3
gibberellic acid
- WPM
woody plant medium 相似文献
15.
Yasseen Mohamed-Yasseen Suzanne Helene Costanza 《In vitro cellular & developmental biology. Plant》1996,32(2):100-102
Summary Two protocols for clonal propagation of kurrat (Allium ampeloprasum var.kurrat) using explants from the basal plates of mature plants are described. In direct formation, explants were cultured in Murashige
and Skoog (MS) medium and supplemented with either benzyladenine at 0.0 or 4.4 μM, or supplemented with 7.0 μM benzyladenine and 0.1 μM naphthaleneacetic acid. Shoots appeared after 4 wk of culture. In the two-step procedure, explants were cultured first on
MS medium supplemented with 1.4 μM 2,4-dichlorophenoxyacetic acid and 1.4 μM kinetin, and incubated in the dark for 4 wk. They were then transferred to MS medium supplemented with 4.4 μM benzyladenine for shoot formation. All shoots were rooted on MS medium containing 5 g·liter−1 activated charcoal. Normal viable plants were successfully established in soil. 相似文献
16.
A protocol for plant regeneration from mesophyll and callus protoplasts of Robinia pseudoacacia L. was developed. For leaves from in vitro raised shoots, an enzyme combination of 2.0% cellulose and 0.3% macerozyme for
a digestion period of 20 h resulted in the best yield of protoplasts (9.45 × 105 protoplast/g fresh weight). Mesophyll-derived protoplasts started cell wall regeneration within 24 h of being embedded in
Nagata and Takebe (NT) medium supplemented with 5 μM NAA and 1 μM BAP followed by the first cell division on day three of
culture and micro-colony (32 cells) formation within day 7–10 in the same medium. However, using callus as the starting material,
a combination of 2.0% cellulose and 1.0% macerozyme for a digestion period of 24 h gave the highest protoplast yield (3.2 × 105 protoplast/g fresh weight). Cell wall regeneration in callus-derived protoplasts started within 24 h followed by the first
cell division on the day three (96 h) and the appearance of microcolonies of more than 32 cells by the end of first week (144 h)
of culture on solid WPM medium supplemented with 5 μM NAA and 1 μM BAP. Microcalli were visible to the naked eye after 45 days
on solid WPM medium. Proliferation of macro-calli was successfully accomplished on solid Murashige and Skoog (MS) medium with
5 μM NAA and 5 μM BAP. Both mesophyll and callus protoplast-derived calli produced shoots on MS medium with 0.5 μM NAA and
1 μM BAP within 25–30 days and multiplied on MS medium with 1.25 μM BAP. Excised microshoots were dipped in 1–2 ml of 2.0 μM
IBA for 24 h under dark aseptic conditions and transferred to double sterilized sand for rooting. The flasks containing sand
were inoculated with Rhizobium for in vitro nodulation. Forty-five plants transferred to pots in the glasshouse established well. 相似文献
17.
Panaia M. Senaratna T. Bunn E. Dixon K.W. Sivasithamparam K. 《Plant Cell, Tissue and Organ Culture》2000,63(1):23-29
A micropropagation protocol was developed for the conservation of the critically endangered Western Australian shrub,Symonanthus bancroftii. It was necessary to screen antioxidant treatments to prevent the occurrence of lethal browning of explants upon excision.
Potassium citrate and citric acid (0.1% w/v in a 4:1 ratio) prevented oxidative browning and was superior to the untreated
control or other antioxidant treatments tested. Half strength Murashige and Skoog (MS) medium containing 0.5 μM kinetin and
0.25 μM benzyladenine produced three-fold multiplication compared to 1.75×, 1.5×, 1.8× and 1× multiplication for 2.5 μM kinetin
+ 0.25 μM benzyladenine, 0.5 μM kinetin + 5 μM gibberellic acid, 1 μM kinetin + 3 μM gibberellic acid and half strength MS
with no plant growth regulators, over 4 weeks. Root production was achieved with indole-3-butyric acid (IBA) and α-naphthaleneacetic
acid (NAA) at 0.5/0.5 μM (31% rooting) and 1.0/1.0 μM (36% rooting), after four weeks. Paclobutrazol (PBZ) at 0, 3.4 (1 mg
1−1), 10.2 (3 mg 1−1), or 17 μM (5 mg 1−1) improved tolerance to desiccation after transfer ofin vitro rooted shoots to soil. PBZ at 10.2 μM increased survival to 90% compared to 50% for those plantlets not treated with PBZ.
The acclimatisation period from the glasshouse to the shadehouse was 1 week for plantlets treated with PBZ compared to 4 weeks
for plantlets without any PBZ. PBZ at 3.4 μM increased the number of roots per shoot compared to untreated controls.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
18.
Cao Dinh Hung Krystyna Johnson Fraser Torpy 《In vitro cellular & developmental biology. Plant》2006,42(6):548-552
Summary An efficient protocol for in vitro propagation of the valuable medicinal plant, Wasabia japonica (Miq.) Matsumura is described through shoot tip proliferation and direct regeneration. Multiple shoots were induced from
shoort tips cultured on Murashige and Skoog (MS) semi-solid medium containing various concentrations (0.5–50 μM) of N6-benzyladenine (BA), thidiazuron, kinetin, and zeatin. A comparison was made on shoot multiplication between semi-solid and
liquid culture media. Well-developed shoots were obtained using full-strength MS semi-solid medium containing 5.0 μM BA. However, the greatest shoot proliferation was achieved on either full- or half-strength MS liquid media supplemented
with 5.0 μM BA for 4 wk (15.3±0.9 and 15.0±0.7 shoots per explant, respectively), and on half-strength MS liquid medium for 6 wk (25.8±1.3
shoots per explant) in culture. In contrast, the maximum number of shoots per explant on full-strength MS semi-solid medium
was achieved with either 5.0 μM BA (10.4±0.6 shoots per explant) or 10.0 μM kinetin (10.9±0.8 shoots per explant). Fresh weight of explants and length of shoots derived from full-strength MS liquid
medium (1055±77 mg and 34.2±1.0 mm, respectively) were significantly higher than those derived from full-strength MS semisolid
medium (437.6±17.3 mg and 15.4±0.7 mm, respectively). Quarter-strength MS liquid medium had no significant difference in shoot
proliferation when compared to quarter-strength MS semi-solid medium. Elongated shoots were separated and rooted on half-strength
MS semi-solid media fortified with 1-naphthaleneacetic acid (NAA), indole-3-butyric acid (IBA), or indole-3-acetic acid (IAA)
ranging from 0.1 to 10.0 μM. Root formation was greatest with IBA when compared with IAA and NAA. One hundred percent of shoots were rooted on half-strength
MS medium with 5.0 μM IBA, while vigorous roots were obtained with 10.0 μM IBA. Micropropagated plantlets were successfully established in soil with 95% survival rate after heardening. 相似文献
19.
María del Socorro Santos Díaz Candy Carranza Álvarez 《In vitro cellular & developmental biology. Plant》2009,45(2):162-170
Two procedures for the in vitro propagation of Encyclia mariae, a threatened Mexican orchid, were developed. In the first procedure, leaves from in vitro germinated seedlings were cultured on Murashige and Skoog medium (MS) supplemented with the range of 2.21–4.4 μM 6-benzylaminopurine
(BA) in combination with 2.69–10.74 μM naphthalene acetic (NAA), 2.07–8.29 μM indole-3-butyric (IBA), or 2.85–11.42 μM indole-3-acetic
acid (IAA) to determine the best medium for the induction of shooting. Maximum direct shoot formation from leaves was observed
on MS containing 22.21 μM BA and 10.74 μM NAA (25 shoots/explant). The second procedure began with the culture of protocorms
on media containing NAA, IBA, or IAA, which induced callus formation with high regenerative potential in the form of protocorm-like-bodies
(PLBs) that eventually differentiated into shoots. The optimal response was attained when these structures were cultured on
medium with 4.14 μM IBA (30 shoots/PLB). To promote the elongation of shoots derived from PLBs, the material was subcultured
onto MS medium containing 22.21 μM BA and 5.37 μM NAA. Through the exploration of the effects of auxins and matrix on the
rooting of shoots, it was determined that the optimal rooting occurred on media supplemented either with 5.71 μM IAA or 4.14 μM
IBA either on agar-gelled medium or in liquid media with coir as the matrix. Rooting was found to be 20% higher in liquid
media than in agar-gelled medium. 相似文献
20.
Stem explants obtained from a mature tree of Ziziphus mauritiana Lamk were grown on modified Murashige and Skoog medium containing 3800 mg l-1 potassium nitrate, 2475 mg l-1 ammonium nitrate, 11 M benzyladenine and 0.5 M indole-3-acetic acid. During successive subcultures 15–20 shoots per inoculum were produced. Rooting was induced by pretreatment with 50 M indolebutyric acid or 1-naphthaleneacetic acid for 24 h followed by transfer to auxin-free White's medium. Plantlets grew well in a soil and vermiculite mixture.Abbreviations IAA
Indole-3-acetic acid
- NAA
1-naphthaleneacetic acid
- BA
benzyladenine
- MS
Murashige and Skoog 相似文献