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Ton Gloudemans Sacco de Vries Henk-Jan Bussink Nasir S. A. Malik Henk J. Franssen Jeanine Louwerse Ton Bisseling 《Plant molecular biology》1987,8(5):395-403
In vitro translation products of total RNA isolated from soybean nodules at successive stages of nodule development were analyzed by two-dimensional gel electrophoresis. In that way the occurrence of over 20 mRNAs specifically transcribed from nodulin genes was detected. The nodulin genes could be divided into two classes according to the time of expression during nodule development. Class A comprises at least 4 nodulin mRNAs which are found when a globular meristem is present in the root cortex. These class A nodulin genes have a transient expression. Class B nodulin genes are expressed when the formation of a nodule structure has been completed. Bradyrhizobium japonicum nod
+ fix-mutants, with large deletions spanning the nif H,DK region, still induced nodules showing normal expression of all nodulin genes, indicating that the nif H,DK region is not involved in the induction of nodulin genes. In nodules induced by Bradyrhizobium japonicum nod
+ fix-mutant HS124 the bacteria are rarely released from the infection thread and the few infected cells appear to be collapsed. All class A and class B nodulin genes are expressed in HS124 nodules with the exception of 5 class B genes. 相似文献
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Cloning and functional characterization of an O-acetylserine(thiol)lyase-encoding gene in wild soybean (Glycine soja) 总被引:2,自引:0,他引:2
The terminal step of soybean cysteine synthesis is catalyzed by O-acetylserine(thiol)lyase (OAS-TL, EC 2.5.1.47). In this study, we isolated and characterized an OAS-TL gene from a wild soybean material (designated as GsOAS-TL1). GsOAS-TL1 cDNA sequence showed strict conservation at both nucleotide and amino acid levels compared with that from cultivated soybean.
Genomic structure analysis of GsOAS-TL1 indicated that it contained 10 exons and 9 introns in the coding region with conserved exon sizes and intron locations compared
with Arabidopsis thaliana
OAS-TL-like genes. Among the complete GsOAS-TL1 cDNA and three part-deletion fragments, only expression of the full-length cDNA could rescue the NK3 cys−
Escherichia coli auxotroph, which was coherent with the assayed enzyme activity of purified fusion proteins. For RT-PCR analysis in different
wild soybean tissues, GsOAS-TL1 showed lower expression in roots and developing seeds, whereas total OAS-TL activity of corresponding tissues showed significantly
higher level in seeds than other tissues. To our knowledge, this is the first report on cloning and characterization of an
OAS-TL gene from wild soybean. Our results are informative to further elucidate the function and evolution of OAS-TL in soybean. 相似文献
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C. B. Singh M. A. Dalal S. P. Singh 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1978,52(4):165-169
Summary Genetic analysis for germination percentage was carried out in the F3 and F4 generations of a diallel cross involving six promising genotypes of soybean. Results indicated a high amount of genetic variability and a moderately high heritability together with genetic advance, suggesting a possible improvement for this character through hybridization and selection. Correlations at different levels revealed a strong negative association of germination with only one seed character: seed weight. This observation was further confirmed from path coefficient analysis. These findings strongly suggest that to base selection on seed weight which may not influence the seed quality of soybean. 相似文献
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The possible ameliorative effects of quercetin on soybean [Glycine max (L.) Merr.] leaves exposed to UV-B radiation were conducted in greenhouse. The symmetrical leaves supplied with quercetin
solution (0.2%, 1%) were exposed to UV-B radiation (0, 3.5, 6.5 kJ m−2 d−1). 0.2% quercetin ameliorated leaf photosynthesis, improved leaf water content (LWC), and decreased lipid oxidation. The unfavorable
effect on photosynthetic parameter was displayed in 1% quercetin treatment. The effect of quercetin on phenylalanine ammonia
lyase (PAL) activity varied with the quercetin concentration, UV-B radiation intensity and leaf development. In the later
development polyphenol oxidase (PPO) activity was increased significantly by quercetin treatments. We suggested that quercetin
with suitable concentration could serve as UV-B protective agent partly due to its antioxidant capacity. 相似文献
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Summary Azospirillum was associated with nodules of soybean. In general, seed inoculation with a broth culture ofAzospirillum brasilense alone significantly increased nodulation and grain yield of soybean grown in pots in unsterilized soil with different levels of urea ranging from 0 to 80 kg N/ha. This trend was significantly reproducible in a second experiment when a carrier based inoculant of the bacterium was used for seed inoculation.Inoculation withRhizobium japonicum andA. brasilense in combination generally increased grain yield in both the experiments, although the data were not significant. 相似文献
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Alkharouf NW Klink VP Chouikha IB Beard HS MacDonald MH Meyer S Knap HT Khan R Matthews BF 《Planta》2006,224(4):838-852
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G. te Kronnie Henri Stroband Henk Schipper Johannis Samallo 《Development genes and evolution》1999,209(7):443-446
The Zfcth1 gene is, as the previously cloned carp cth1 gene, related to the mammalian TIS 11 family of primary response genes and encodes a protein with two putative CCCH zinc fingers. This report describes the RNA
expression of this gene during oogenesis and early embryogenesis up to gastrulation in the zebrafish (Danio rerio). Maternal cth1 message is present in the ovary of 1-month-old fish and of adult fish in oocytes at all stages of maturation. In the youngest
oocytes the message is localized in the cytoplasm all around the nucleus, in larger oocytes the message becomes restricted
to the future animal pole of the embryo, and in mature oocytes the expression is sharply localized in the cortical layer under
the micropyle. After ovulation the cth1 messenger spreads over the cytoplasmic cap and is distributed over the blastomeres during subsequent cleavages. In subsequent
stages maternal expression of cth1 gradually disappears. From early epiboly stages onward embryonic cth1 expression is localized to the germ ring and the hypoblast cells in the central part of the embryonic shield. In the shield, cth1 expression largely overlaps with the area of gooscoid expression in the first involuting cells. In stages after 70% of epiboly cth1 expression diminishes and soon can no longer be detected in the embryo. Next to a developmental role in cell fate determination
we propose a function for cth1 during oocyte maturation.
Received: 19 October 1998 / Accepted: 16 February 1999 相似文献
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In situ expression of the GmNMH7 gene is photoperiod-dependent in a unique soybean (Glycine max [L.] Merr.) flowering reversion system 总被引:1,自引:0,他引:1
Soybean is a short-day plant and its flowering process can be reversed when switching from short-day to long-day conditions. Flowering reversion provides a useful system to study the flowering process in both forward and backward directions. In this study, we optimized a soybean flowering reversion system using a photoperiod-sensitive cultivar Zigongdongdou. Three types of terminal structures were found during flowering reversion: reversed terminal raceme (RTR), short terminal raceme (STR), and vegetative terminal (VT). The relative frequency of these terminal structures during flowering reversion under long day was dependent on the duration of the prior short day (SD) pretreatment. This process is phytochrome dependent and young plants were more susceptible to flowering reversion. Leaf removal increased the minimal SD period needed for the induction of STR. To demonstrate the application of this system, we studied the patterns of in situ expression of the GmNMH7 gene during flowering development and reversion. NMH7 family members encode MADS-box proteins and are unique in legume families since their expression can be detected in both developing flowers and nodules. In situ hybridization experiments using plants grown under different photoperiod cycles provided several lines of evidence supporting a close relationship between GmNMH7 gene expression and floral development in soybean. Furthermore, it seems that GmNMH7 may participate in flower development at different stages. Interestingly, the expression pattern of GmNMH7 in root nodules was also found to be regulated by photoperiod. These results support the notion that the photoperiod sensitive GmNMH7 gene may play multiple roles in growth and development in soybean.C. Wu and Q. Ma contributed equally to this work. 相似文献
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Guruprasad K Kadur G Bhattacharjee S Swapan B Kataria S Sunita K Yadav S Sanjeev Y Tiwari A Arjun T Baroniya S Sanjay B Rajiv A Abhinav R Mohanty P 《Photosynthesis research》2007,94(2-3):299-306
Exclusion of UV (280–380 nm) radiation from the solar spectrum can be an important tool to assess the impact of ambient UV
radiation on plant growth and performance of crop plants. The effect of exclusion of UV-B and UV-A from solar radiation on
the growth and photosynthetic components in soybean (Glycine max) leaves were investigated. Exclusion of solar UV-B and UV-B/A radiation, enhanced the fresh weight, dry weight, leaf area
as well as induced a dramatic increase in plant height, which reflected a net increase in biomass. Dry weight increase per
unit leaf area was quite significant upon both UV-B and UV-B/A exclusion from the solar spectrum. However, no changes in chlorophyll
a and b contents were observed by exclusion of solar UV radiation but the content of carotenoids was significantly (34–46%) lowered.
Analysis of chlorophyll (Chl) fluorescence transient parameters of leaf segments suggested no change in the F
v/F
m value due to UV-B or UV-B/A exclusion. Only a small reduction in photo-oxidized signal I (P700+)/unit Chl was noted. Interestingly the total soluble protein content per unit leaf area increased by 18% in UV-B/A and 40%
in UV-B excluded samples, suggesting a unique upregulation of biosynthesis and accumulation of biomass. Solar UV radiation
thus seems to primarily affect the photomorphogenic regulatory system that leads to an enhanced growth of leaves and an enhanced
rate of net photosynthesis in soybean, a crop plant of economic importance. The presence of ultra-violet components in sunlight
seems to arrest carbon sequestration in plants.
An erratum to this article can be found at 相似文献
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Manganese and iron interactions on their uptake and distribution in soybean (Glycine max (L.) Merr.)
Summary The uptake and distribution of iron and manganese were studied in a manganese-sensitive soybean cultivar (‘Bragg’) grown over
a range of supply levels of these nutrients in solution culture. At high (90 and 275 μM) manganese levels, increasing the iron concentration in solution from 2 to 100 μM partially overcame the effects of manganese toxicity. Interactions between manganese and iron occurred for dry matter yields,
rate of Mn absorption by the roots, and the proportions of manganese and iron transported to the tops. No interaction was
observed for the rate of root absorption of iron.
The percentage distribution of manganese in the plant top increased with increasing iron, despite a reduced rate of Mn uptake.
On the other hand, iron uptake was independent of solution Mn concentration and increased with increasing solution Fe. Also
more iron was retained in the roots at high Mn and/or Fe levels in solution.
Concentrations of manganese and iron in roots, stems and individual leaves were affected independently by the manganese and
iron supplyi.e. without any interaction occurring between the two elements. In general, the concentration in a plant part was related directly
to the solution concentration. Symptoms resembling iron deficiency correlated poorly with leaf Fe concentrations whereas high
levels of manganese were found in leaves displaying Mn toxicity symptoms. 相似文献
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Huang Y Tang R Dai J Gu S Zhao W Cheng C Xu M Zhou Z Ying K Xi Y Mao Y 《Molecular biology reports》2001,28(4):185-191
We report the cloning and characterization of a novel human hydroxysteroid dehydrogenase like gene (HSDL1) located on human chromosome 16q24.2. The HSDL1 cDNA is 3407 base pair in length, encoding a 309 amino acid polypeptide related to human 17-HSD3. Northern blot reveals that the HSDL1 is highly expressed in testis and ovary. In situ hybridization indicates that the expression of HSDL1 is predominantly increased in the prostate cancer tissue compared with the normal prostate tissue, which suggests that the gene expression is important to the arising of prostate cancer. 相似文献
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