Production and decomposition processes in a saline meromictic lake

Bacterial and phytoplankton cell number and productivity were measured in the mixolimnion and chemocline of saline meromictic Mahoney Lake during the spring (Apr.–May) and fall (Oct.) between 1982 and 1987. High levels of bacterial productivity (methyl ³H-thymidine incorporation), cell numbers, and heterotrophic assimilation of ¹⁴C-glucose and ¹⁴C-acetate in the mixolimnion shifted from near surface (1.5 m), at a secondary chemocline, to deeper water (4–7 m) as this zone of microstratification gradually weakened during a several year drying trend in the watershed. In the mixolimnion, bacterial carbon (13–261 µgC 1^–1) was often similar to phytoplankton carbon (44–300 µgC 1^–1) and represented between 14–57% of the total microbial (phytoplankton + bacteria) carbon depending on the depth interval. Phototrophic purple sulphur bacteria were stratified at the permanent primary chemocline (7.5–8.3 m) in a dense layer (POC 250 mg 1^–1, bacteriochlorophyll a 1500–70001µ 1^–1), where H₂S changed from 0.1 to 2.5 mM over a 0.2 m depth interval. This phototrophic bacterial layer contributed between 17–66% of the total primary production (115–476 mgC m^–2 d^–1) in the vertical water column. Microorganisms in the phototrophic bacterial layer showed a higher uptake rate for acetate (0.5–3.7 µC 1^–1 h^–1) than for glucose (0.3–1.4 µgC 1^–1 h^–1) and this heterotrophic activity as well as bacterial productivity were 1 to 2 orders of magnitude higher in the dense plate than in the mixolimnetic waters above. Primary phytoplanktonic production in the mixolimnion was limited by phosphorus while light penetration appeared to regulate phototrophic productivity of the purple sulphur bacteria.     

Photosynthetic bacteria in meromictic lakes and stratified fjords of the Vestfold Hills,Antarctica

Thirteen meromictic lakes and two permanently stratified fjords in the Vestfold Hills, Antarctica, were surveyed in 1983 for photosynthetic bacteria. Burton Lake and Ellis Fjord were sampled throughout the year to determine seasonal variations. Physical and chemical parameters were recorded and related to the species present. The dominant species in waters with salinities of 100.7 g kg^–1 were Chlorobium vibrioforme and Chlorobium limicola with populations at the O₂–H₂S interface in the range 0.3 to 6.7 × 10⁶ ml^–1. Neither of these species was found at higher salinities. Thiocapsa roseopersicina and a Chromatium sp. were found in low numbers (< 10⁵ ml^–1) in most of the same waters as the Chlorobium spp. These bacterial phototrophs developed in a narrow band below the O₂–H₂S interface where both light and H₂S were available. Very low numbers (< 10² ml^–1) of Rhodopseudomonas palustris were found in both oxic and anoxic waters having salinity 148 g kg^–1. The dominance of the Chlorobium spp. is ascribed to their more efficient maintenance metabolism during the darkness, their faster growth at low light intensities (< 1 µE m^–2 s^–1) and the lack of selective filtering of incident light. The Chlorobium spp. grew well at –2 °C, but not –5°C in hypersaline waters. The concentration of H₂S had no apparent effect on the development of the bacterial flora. Viable cells were found to depths of 100 m in Ellis Fjord indicating that viability in total darkness could have been maintained for periods of the order of 1700 days.     

Phytoplankton primary production in a shallow,well- mixed,hypertrophic South African lake

This paper reports on a two-year analysis of the wind climateand its effect on phytoplankton primary production in ashallow (mean depth = 1.9 m), hypertrophic South Africancoastal lake, Zeekoevlei. The lake is subject to continuousmixing of the euphotic zone (Z _eu = 0.8 m), andcomplete mixing of the water column to the mean depth on adaily basis. Median annual wind speeds, prevailing fromeither the north or the south, were 6.4 m s^–1. There wasan almost total absence of calms, measured as hourly meanwind speeds of <1 m s^–1. Notwithstanding the highfrequency of mixing, the lake supports a dense population ofphytoplankton, dominated by Cyanophyte and Chlorophytespecies. Mean concentrations of chlorophyll-a were240 g l^–1. The attenuation of photosyntheticallyavailable radiation, PAR, was high, with mean K _dvalues of 6.4 m^–1 and water transparencies of <0.5 m.Levels of primary productivity, determined using the lightand dark bottle oxygen method, were very high, comparable toor exceeding that of the most productive systems yet studied.Maximum volumetric productivity ranged from 525 to 1524 mg Cm^–3 h^–1, and was confined to the upper 0.5 m of thewater column. Daily areal productivity, P _d,varied between 1.2 and 4.3 g C m^–2 d^–1, and that ofthe maximum chlorophyll-a specific photosynthetic rate,P _{B max}, between 1.6 and 7.9 mg C (mgChl-a)^–1 h^–1. Primary production was limited bywater temperature and the attenuation of PAR. The highfrequency of wind-induced mixing resulted in regular mixingof the phytoplankton through the euphotic zone, and reducedthe overall importance of P _max at a single layer inthe depth profile. Similarly, the regularity of mixing wasrecognized as a limitation of the incubation of bottle chainsto determine primary production levels.     

Optimization of eicosapentaenoic acid production byPhaeodactylum tricornutumin the flat panel airlift (FPA) reactor

Biomass and eicosapentaenoic acid (EPA) productivities were investigated in a flat panel airlift loop reactor ideally mixed by static mixers. Growth with ammonium, urea and nitrate as nitrogen source were performed at different aeration rates. Cultures grew on ammonium but the decay of pH strongly inhibited biomass increase. On urea biomass productivity reached 2.35 g L^–1d^–1at an aeration rate of 0.66 vvm (24 h light per day, 1000 mol photon m^–2s^–1). Aeration rates between 0.33 vvm and 0.66 vvm and maximal productivities on urea were linearly dependent. Productivity on nitrate never exceeded 1.37 g L^–1d^–1. In the range of maximum productivity photosynthesis efficiency of 10.6% was reached at low irradiance (250 mol photon m^–2s^–1). Photosynthesis efficiency decreased to 4.8% at 1000 mol photon m^–2s^–1. At these high irradiances the flat panel airlift reactor showed a 35% higher volume productivity than the bubble column. At continuous culture conditions the influence of CO₂concentration in the supply air was tested. Highest productivities were reached at 1.25% (v/v) CO₂where the continuous culture yielded 1.04 g L^–1d^–1(16 h light per day, 1000 mol photon m^–2s^–1). The average EPA content amounted to 5.0% of cell dry weight, that resulted in EPA productivities of 52 mg L^–1d^–1(continuous culture, 16 h light per day) or 118 mg L^–1d^–1(batch culture, 24 h light per day).     

The organic carbon budget of a shallow arctic tundra lake on the Tuktoyaktuk Peninsula,N.W.T., Canada

The organic carbon cycle of a shallow, tundra lake (mean depth 1.45 m) was followed for 5 weeks of the open water period by examining CO₂ fluxes through benthic respiration and anaerobic decomposition, photosynthesis of benthic and phytoplankton communities and gas exchange at the air-water interface. Total photosynthesis (as consumption of carbon dioxide) was 37.5 mmole C m^–2 d^–1, 83% of which was benthic and macrophytic. By direct measurement benthic respiration exceeded benthic photosynthesis by 6.6 mmole C m^–2 d^–1. The lake lost 1.4 × 10⁶ moles C in two weeks after ice melted by degassing C0₂, and 6.8 mmole C m^–2 d^–1 (1.5 × 10⁶ moles) during the remainder of the open water period; 2.2 mmole C m² d^–1 of this was release Of CO₂ stored in the sediments by cryoconcentration the previous winter. Anaerobic microbial decomposition was only 4% of the benthic aerobic respiration rate of 38 mmole C m^–2 d^–1. An annual budget estimate for the lake indicated that 50% of the carbon was produced by the benthic community, 20% by phytoplankton, and 30% was allochthonous material. The relative contribution of allochthonous input was in accordance with measurement of the ¹⁵N of sedimented organic matter.     

Photosynthetic activity of phytoplankton in a high altitude lake (Emerald Lake,Sierra Nevada,California)

Photosynthetic activity by phytoplankton was measured during the ice-free seasons of 1984, 1985 and 1987 using the ¹⁴C radioassay in high altitude Emerald Lake (California). Relative quantum yield (^B) and light-saturated chlorophyll-specific carbon uptake (P_m ^B) were calculated from the relationship of light and photosynthesis fitted to a hyperbolic tangent function. Temporal changes in P_m ^B showed no regular pattern. Seasonal patterns of ^B generally had peaks in the summer and autumn. Phytoplankton biomass (as measured by chlorophyll a) and light-saturated carbon uptake (P_m) had peaks in the summer and autumn which were associated with vertical mixing. Estimates of mean daily carbon production were similar among the three years: 57 mg C m^–2 2 d^–1 in 1984, 70 mg C m^–2 2 d^–1 in 1985 and 60 mg C m^–2 d^–1 in 1987. Primary productivity in Emerald Lake is low compared to other montane lakes of California and similar to high-altitude or high-latitude lakes in other regions.     

Continuous underwater light measurement near Helgoland (North Sea) and its significance for characteristic light limits in the sublittoral region

Underwater irradiance was measured at intervals of 20 min for one year at 2 water depths (2.5 and 3.5 m below M.L.W.S.) and in 3 spectral regions in the sublittoral region of the rocky island of Helgoland. Data are presented for spectral and total irradiance at water depths ranging from 2 to 15 m (below M.L.W.S.). 90% of the total annual light reaching sublittoral habitats is received during the period from April to September, when Jerlov water type 7 (occasionally water type 5) dominates. During the other half of the year, the water is very turbid, and transparency is so low that long dark periods occur even at moderate water depths. The total annual light received at the lower kelp limit (Laminaria hyperborea), at 8 m water depth, is 15 MJ m^–2 year^–1 or 70 E m^–2 year^–1, which corresponds to 0.7% of surface irradiance (visible). At the lower algal limit (15 m water depth) these values are 1 MJ m^–2 year^–1 or 6 E m^–2 year^–1, corresponding to 0.05% of surface irradiance. These data are similar to measurements at the same limits in several different geographical areas, and may determine the depth at which these limits occur.     

Benthic fluxes in San Francisco Bay

Measurements of benthic fluxes have been made on four occasions between February 1980 and February 1981 at a channel station and a shoal station in South San Francisco Bay, using in situ flux chambers. On each occasion replicate measurements of easily measured substances such as radon, oxygen, ammonia, and silica showed a variability (±1) of 30% or more over distances of a few meters to tens of meters, presumably due to spatial heterogeneity in the benthic community. Fluxes of radon were greater at the shoal station than at the channel station because of greater macrofaunal irrigation at the former, but showed little seasonal variability at either station. At both stations fluxes of oxygen, carbon dioxide, ammonia, and silica were largest following the spring bloom. Fluxes measured during different seasons ranged over factors of 2–3, 3, 4–5, and 3–10 (respectively), due to variations in phytoplankton productivity and temperature. Fluxes of oxygen and carbon dioxide were greater at the shoal station than at the channel station because the net phytoplankton productivity is greater there and the organic matter produced must be rapidly incorporated in the sediment column. Fluxes of silica were greater at the shoal station, probably because of the greater irrigation rates there. N + N (nitrate + nitrite) fluxes were variable in magnitude and in sign. Phosphate fluxes were too small to measure accurately. Alkalinity fluxes were similar at the two stations and are attributed primarily to carbonate dissolution at the shoal station and to sulfate reduction at the channel station. The estimated average fluxes into South Bay, based on results from these two stations over the course of a year, are (in mmol m^–2 d^–1): O₂ = –27 ± 6; TCO₂ = 23 ± 6; Alkalinity = 9 ± 2; N + N = –0.3 ± 0.5; NH₃ = 1.4 ± 0.2; PO₄ = 0.1 ± 0.4; Si = 5.6 ± 1.1. These fluxes are comparable in magnitude to those in other temperate estuaries with similar productivity, although the seasonal variability is smaller, probably because the annual temperature range in San Francisco Bay is smaller.Budgets constructed for South San Francisco Bay show that large fractions of the net annual productivity of carbon (about 90%) and silica (about 65%) are recycled by the benthos. Substantial rates of simultaneous nitrification and denitrification must occur in shoal areas, apparently resulting in conversion to N₂ of 55% of the particulate nitrogen reaching the sediments. In shoal areas, benthic fluxes can replace the water column standing stocks of ammonia in 2–6 days and silica in 17–34 days, indicating the importance of benthic fluxes in the maintenance of productivity.Pore water profiles of nutrients and Rn-222 show that macrofaunal irrigation is extremely important in transport of silica, ammonia, and alkalinity. Calculations of benthic fluxes from these profiles are less accurate, but yield results consistent with chamber measurements and indicate that most of the NH₃, SiO₂, and alkalinity fluxes are sustained by reactions occurring throughout the upper 20–40 cm of the sediment column. In contrast, O₂, CO₂, and N + N fluxes must be dominated by reactions occurring within the upper one cm of the sediment-water interface. While most data support the statements made above, a few flux measurements are contradictory and demonstrate the complexity of benthic exchange.     

Benthic oxygen flux in the highly productive subarctic Lake Myvatn, Iceland: In situ benthic flux chamber study

In situ paired light and dark-stirred benthic flux chambers were used to estimate dissolved oxygen flux across the sediment–water interface in Lake Mývatn, Iceland. Three sampling stations were selected, each station reflecting a specific sedimentary environment, benthic communities, and water depth. During this study the phytoplankton density was low. Spatial and seasonal variations of bottom DO concentration and DO flux have been observed during this study. The oxygen consumption rate at all study sites had a mean of –89 (±44) mmol m^–2 d^–1 while the oxygen production rate due to benthic algae had a mean of 131 (±103) mmol m^–2 d^–1. There was a strong correlation (r=0.91) between oxygen consumption rate and temperature. This was presumably because of the temperature influence on rates of microbial and macrobenthic processes. The mean benthic primary production rate at all study sites was 1216 (±957) mg C m^–2 d^–1 between June 2000 and February 2001. Annual gross benthic primary production was estimated from the gross mean daily benthic DO production (P) and Redfield's C:O₂ ratio of 106:138 to be 420 g C m^–2 y^–1 at station HO, 250 g C m^–2 y^–1 at B2 and 340 g C m^–2 y^–1 at station 95. Thus, the mean gross benthic primary production was estimated as 1151 mg C m^–2 d^–1 at station HO, 685 mg C m^–2 d^–1 at station B2, and 932 mg C m^–2 d^–1 at station 95.     

Winter microbial activity in Lake Tuusulanjärvi

Microbial heterotrophic activity, dark CO₂ assimilation, primary productivity and microbial ATP were measured monthly in the extremely eutrophic Lake Tuusulanjärvi during the winter of 1979–1980. Because of continuous water circulation caused by low temperature and artificial aeration of the lake, no winter stratification developed. Very low summertime ³H-glucose turnover times of 5 h increased to a level of 10–20 h from August to January. Winter maximum of 110 h was measured in March, and turnover times returned to 10–20 h in April, before the vernal bloom of algae occured. Oxygen saturation remained over 46% during the winter.High primary productivity was observed in November (400–500 mg C m^–3 day^–1), and measurable productivity was detected under ice in January (80 mg C m^–3 day^–1). Dark CO₂ assimilation increased to 14% of primary productivity in March. No correlation was found between ³H-glucose turnover rate and dark CO₂ assimilation. ATP correlated slightly better with primary productivity than with turnover rate. The single concentration method proved to be sensitive for winter heterotrophic activity measurement.     

Heterotrophic bacterial activity in Roskilde Fjord sediment during an autumn sedimentation peak

Total oxygen uptake, bacterial oxygen uptake, total bacterial biomass and active bacterial biomass were determined at the sediment-water interface at two stations in the brackish Roskilde Fjord between September and December in 1986 before, during and after sedimentation of a phytoplankton bloom. Bacterial oxygen consumption was separated from total oxygen consumption by addition of cycloheximide. The fractional and the absolute bacterial oxygen uptake were greatest at the most eutrophic station, where total oxygen uptake was 870–1740 mg O₂ m^–2 d^–1 and the bacterial oxygen uptake was 232–870 mg O₂ m^–2 d^–1. At the less eutrophic station, total oxygen uptake was 725–1740 mg O₂ m^–2 d^–1. and bacterial oxygen uptake was 200–550 mg O₂ m^–2 d^–1.Active bacterial biomass was separated from total bacterial biomass by addition of the terminal electron acceptor INT-formazan. The active bacterial biomass was 70–120 µg C mg^–1 ww of sediment at the most eutrophic station and 50–90 µg C g^–1 ww of sediment at the other station. Differences in capacity of bacterial oxygen uptake between the two stations correlated to the active bacterial biomass. The non-temperature dependent bacterial oxygen uptake correlated with the sedimentation rate.     

The summer zooplankton community at South Georgia: biomass,vertical migration and grazing

Zooplankton abundance and biomass were determined during January 1990 at two stations to the north-west of South Georgia using a Longhurst Hardy Plankton Recorder (LHPR). At both shelf and oceanic station sites, zooplankton biomass, (excluding Euphausia superba), was found to be ca. 13 g dry mass m^–2. Copepods and small euphausiids dominated the catches. These estimates are over 4 times higher than values generally reported for the Southern Ocean and may reflect firstly, the high productivity of the study area, secondly, the time of year, summer, when biomass for many species is maximal, and thirdly, the high sampling efficiency of the LHPR. Principal components analysis disclosed similarities and differences between adjacent depth strata in terms of abundance, biomass and species composition. At both stations most variability occurred in the mixed layer (0–60 m) and thermocline (60–120 m) with depth horizons below this being more homogeneous. Diel migrations were observed for most taxa with abundance increasing in the mixed layer at night. At the oceanic station, species and higher taxa belonging to the mesopelagic community were generally well spread throughout this domain and, with the exception of Pleuromamma robusta and Metridia curticauda, showed little evidence of migration. The grazing impact of the epipelagic community (copepods and small euphausiids) was estimated to remove 3–4% of the microbial standing stock day^–1 and a conservative 25% and 56% of daily primary production at the oceanic and shelf stations respectively.     

Annual productivity of Spirulina (Arthrospira) and nutrient removal in a pig wastewater recycling process under tropical conditions

An evaluation was made of the annual productivity of Spirulina (Arthrospira) and its ability to remove nutrients in outdoor raceways treating anaerobic effluents from pig wastewater under tropical conditions. The study was based at a pilot plant at La Mancha beach, State of Veracruz, Mexico. Batch or semi-continuous cultures were established at different seasons during four consecutive years. The protein content of the harvested biomass and the N and P removal from the ponds were also evaluated. Anaerobic effluents from digested pig waste were added in a proportion of 2% (v/v) to untreated sea-water diluted 1:4 with fresh water supplemented with 2 g L^–1 sodium bicarbonate, at days 0, 3 and 5. A straight filament strain of Spirulina adapted to grow in this complex medium was utilized. A pH value 9.5 ± 0.2 was maintained. The productivity of batch cultures during summer 1998 was significantly more with a pond depth of 0.10 m than with a depth 0.065 m. The average productivity of semi-continuous cultures during summer 1999 was 14.4 g m^–2 d^–1 with a pond depth of 0.15 m and 15.1 g m^–2 d^–1 with a depth of 0.20 m. The average annual productivity for semi-continuous cultures operating with depths of 0.10 m for winter and 0.15 and 0.25 m for the rest of the year, was 11.8 g m^–2 d^–1. This is the highest value reported for a Spirulina cultivation system utilising sea-water. The average protein content of the semi-continuous cultures was 48.9% ash-free dry weight. NH₄-N removal was in the range 84–96% and P removal in the range of 72–87%, depending on the depth of the culture and the season.     

The effects of temperature acclimation on the photoinhibitory responses of Ulva rotundata Blid.

The effect of acclimation to 25, 18, or 10° C on the relationship between photoprotection and photodamage was tested in low-light-grown (80 mol · m^–2 · s^–1) Ulva rotundata Blid. exposed to several higher irradiances at the acclimation temperature. Changes in chlorophyll fluorescence parameters (minimum fluorescence, F₀, and the ratio of variable to maximum fluorescence, F_v/F_m, measured after 5 min darkness) were monitored during 5 h transfers to 350, 850, and 1700 mol · m^–2 · s^–1, and during recovery after 1- or 5-h treatments. At all temperatures, rate of onset and final extent of photoinhibition, measured by a decrease in F_v/F_m, increased with increasing irradiance. At a given photoinhibitory irradiance, rate of onset was most rapid at 10 ° C, but the extent was temperature-independent. Recovery rates from mild light stress were similar at all temperatures, but recovery from the most extreme photoinhibitory treatment lagged 2 h at 10° C. De-epoxidation of xanthophyll-cycle components proceeded faster and to a lower epoxidation status at 25° C, but there was little difference in the pool size among the three growth conditions. Using chloramphenicol to inhibit chloroplast protein synthesis and dithiothreitol to inhibit violaxanthin de-epoxidation, it was shown that at the lowest light treatment given, the extent of photoinhibition could be attributed both to greater amounts of photodamage and to greater zeaxanthin-related photoprotection at 25 than at 10° C. While these two mechanisms for high-light-induced loss of photosynthetic efficiency were operating at 10° C, there was evidence for a relatively greater proportion of zeaxanthin-unrelated photoprotection at the low temperature. This photoprotective mechanism is related to a rapidly reversible increase in F₀ and is insentivite to both chloramphenicol and dithiothreitol.Abbreviations and Symbol CAP chloramphenicol - DTT dihiothreitol - F₀, F_m, F_v minimum, maximum, and variable fluorescence - quantum yield This research was conducted in partial fulfillment of the requirements for the Ph. D. degree in the Department of Botany, Duke University. The author wishes to thank E.-M. Aro, W.J. Henley, G. Levavasseur, C.B. Osmond, and J. Ramus for helpful discussions, and C. Lovelock for pigment standards. Funding was provided by Grants-in-Aid of Research from Sigma Xi and the Phycological Society of America, and a Lynde and Harry Bradley Foundation Fellowship to L.A.F., and National Science Foundation grant OCE-8812157 to C.B.O. and J.R.     

Localization of sulfate reduction in planted and unplanted rice field soil

Rates of in situ sulfate reduction (SRR) in planted and unplanted rice fieldsoil were measured by the ³⁵SO^2– ₄-radiotracermethod using soil microcosms. The concentration of ³⁵SO^2– ₄ decreased exponentially with time.However, time course experiments indicated that incubation times of10–30 min were appropriate for measurements of SRRusing a single time point in routine assays. Unplanted microcosmsshowed high SRR of 177 nmol cm^-3 d^-1 inthe uppermost centimeter where average sulfate concentrations were<33 µM. Fine scaled measurements (1 mmresolution) localized highest SRR (<100 nmol cm^-3d^-1) at the oxic/anoxic interface at 2–5 mmdepth. In planted rice field soil, SRR of <310 nmolcm^-3 d^-1 were observed at 0–2cm depth. Sulfate reduction rates were determined at a millimeter-scalewith distance to a two dimensional root compartment. The SRR was highestat 0–1.5 mm distance to the root layer with rates up to500 nmol cm^-3 d^-1, indicating a highstimulation potential of the rice roots. SRR seemed to be mainlydependent on the in situ sulfate porewater concentrations. At thesoil surface of unplanted microcosms sulfate concentration decreasedfrom <150 µM to <10 µM within the first 8 mm of depth. In planted microcosmssulfate concentration varied from 87–99 µMsulfate at the 0–3 mm distance to the root layer to48–62 µM sulfate at a root distance>4 mm from the roots.The depth distribution of inorganic sulfur compounds was determinedfor planted and unplanted rice field soil. Sulfate, acid volatilesulfide (AVS) and chromium reducible sulfide (CRS) were up to 20 foldhigher in planted than in unplanted microcosms. CRS was the majorinsoluble sulfur fraction with concentrations >1.7µmol cm^-3. Organic sulfur accounted for25–46% of the total sulfurpresent (269 µg/g dw) in an unplanted microcosm.The biogeochemical role of sulfate reduction forshort-term accumulation of inorganic sulfur compounds(FeS, FeS_{_2} and S°) in rice soil wasdetermined in a time course experiment with incubationperiods of 5, 10, 20, 30 and 60 min. The relativedistribution of CRS and AVS formation showedlittle depth dependence, whereas the formation of³⁵S° seemed to be the highest in themore oxidized upper soil layers and near the root surface.AV³⁵S was the first major product of sulfatereduction after 20–30 min, whereas CR³⁵Swas formed, as AV³⁵S and ³⁵S°decreased, at longer incubation periods of >30 min.     

Cycling of inorganic and organic sulfur in peat from Big Run Bog,West Virginia

Total S concentration in the top 35 cm of Big Run Bog peat averaged 9.7 mol·g — wet mass^–1 (123 mol·g dry mass^–1). Of that total, an average of 80.8% was carbon bonded S, 10.4% was ester sulfate S, 4.5% was FeS₂­S, 2.7% was FeS­S, 1.2% was elemental S, and 0.4% was SO₄ ^2–­S. In peat collected in March 1986, injected with³⁵S­SO₄ ^2– and incubated at 4 °C, mean rates of dissimilatory sulfate reduction (formation of H₂S + S⁰ + FeS + FeS₂), carbon bonded S formation, and ester sulfate S formation averaged 3.22, 0.53, and 0.36 nmol·g wet mass^–1·h^–1, respectively. Measured rates of sulfide oxidation were comparable to rates of sulfate reduction. Although dissolved SO₄ ^2– concentrations in Big Run Bog interstitial water (< 200 µM) are low enough to theoretically limit sulfate reducing bacteria, rates of sulfate reduction integrated throughout the top 30–35 cm of peat of 9 and 34 mmol·m^–2·d^–1 (at 4 °C are greater than or comparable to rates in coastal marine sediments. We suggest that sulfate reduction was supported by a rapid turnover of the dissolved SO₄ ^2– pool (average turnover time of 1.1 days). Although over 90% of the total S in Big Run Bog peat was organic S, cycling of S was dominated by fluxes through the inorganic S pools.     

Long-term productivity in the cryptoendolithic microbial community of the Ross Desert,Antarctica

Annual gross productivity of the lichen-dominated cryptoendolithic community was calculated from a computer analysis of photosynthetic response based on laboratory measurements of C0₂ exchange and three years (1985–1988) of field nanoclimate data. Photosynthetic optimum increased from –3 to 2°C between irradiance levels of 100 and 1500 mol photons m^–2 s^–1, while the upper compensation point rose from 1 to 17°C. The mean yearly total time available for metabolic activity (temperature above –10°C and moisture present) was 771.3 h for horizontal rock, 421.5 h for northeast-oriented sloped rock, and 1042.2 h for a small depression in horizontal rock (the characteristic site of occasional lichen apothecia). The calculated mean gross productivity value for a horizontal rock was 1215 mg C m^–2 y^–1, and net photosynthetic gain was 606 mg C m^–2 y^–1. Net ecosystem productivity (annual accretion of cellular biomass) estimated from long-term events amounted to only about 3 mg C m^–2 y^–1. The difference between these two values may represent the long-term metabolic costs of the frequent dehydration-rehydration and freezing-thawing cycles or of overwintering, and may account for the leaching of organic substances to the rock.The yearly gross productivity of the cryptoendolithic microbial community of the entire Ross Desert area was estimated at approximately 120,000–180,000 kg C. Of this, 600–900 kg C is in microbial biomass, and much of the rest is soluble compounds that leach into the rocks and possibly percolate to the valleys, providing a source of organic matter for lakes, rivers, and soils. Offprint requests to: E. I. Friedmann.     

Root distribution,standing crop biomass and belowground productivity in a semidesert in México

In a semidesert community in México (Zapotitlán de las Salinas, Puebla) the vertical distribution of roots and root biomass was estimated at 0–100 cm depth on two sampling dates, November 1995 (wet season) and January 1998 (dry season). Root productivity at 7 to 14.5 cm depth was estimated with the in-growth core technique every two months from March 1996 to February 1998. The relationship between environmental factors and seasonal root productivity was analyzed. Finally, we tested the effect of an irrigation equivalent to 20 mm of rain on root production. Seventy four percent of the total number of roots were found at 0-40 cm depth. Very fine roots (<1 mm diameter) were found throughout the soil profile (0-100 cm). In contrast, fine roots (1-3 mm diameter) were found only from 0–90 cm depth, and coarse roots (>3 mm diameter) from 0–60 cm depth. The root biomass was 971.5 g m^–2 (S.D. = 557.39), the very fine and fine roots representing 62.9% of the total. Total root productivity, as estimated with the ingrowth core technique, was 0.031 Mg ha^–1 over the dry season and 0.315 Mg ha^–1 over the wet season. Only very fine roots were obtained at all sampling dates. Rainfall was significantly correlated with very fine root production. The difference between fine root production in non-watered (0.054 g m^–2) and watered (0.429 g m^–2) treatments was significant. The last value was the same as that predicted for a rain of 20 mm, according to the exponential model describing the relation between the production of very fine roots and rainfall at the site.     

Air Breathing and Gill Ventilation Frequencies in Juvenile Tarpon, Megalops atlanticus: Responses to Changes in Dissolved Oxygen, Temperature, Hydrogen Sulfide, and PH

This study quantified the air-breathing frequency (ABf in breaths h^–1) and gill ventilation frequency (Vf in ventilations min^–1) of tarpon Megalops atlanticusas a function of PO₂, temperature, pH, and sulphide concentration. Ten tarpon held at normoxia at 22–33°C without access to atmospheric oxygen survived for eight days, and seven survived for 14 days (at which point the experiment was terminated) suggesting that the species is a facultative, rather than an obligate, air breather. At temperatures of 29°C and below ABf was highest and Vf was lowest at low oxygen partial pressures. Tarpon appear to switch from aquatic respiration to air breathing at PO₂levels of roughly 40 torr. The gills were the primary organ for oxygen uptake in normoxia, and the air-breathing organ the primary mechanism for oxygen uptake in hypoxia. At 33°C, both ABf and Vf were elevated but highly variable, regardless of PO₂. There were no mortalities in tarpon exposed to total H₂S concentrations of 0–232µM (0–150.9µM H₂S); however, high sulfide concentrations resulted in very high ABf and Vf near zero. Vf was reduced when pH was acidic. We conclude that air breathing provides an effective means of coping with the environmental conditions that characterize the eutrophic ponds and sloughs that juvenile tarpon typically inhabit.     

Pyrite accumulation in salt marshes in the Eastern Scheldt,southwest Netherlands

Pore water composition, pyrite distribution and pyrite crystal morphology of sediments from salt marshes in the Eastern Scheldt, southwestern Netherlands, were examined from July 1984 to October 1986.Hydrology and marsh vegetation were the chief determinants of pyrite accumulation. In the bare sediments of pans in the low marsh, highly reducing conditions prevailed just below the surface. At these sites, practically all the incoming detrital pyrite (0.5–1% FeS₂) was preserved. The in-situ formation of pyrites was negligible in these anoxic sediments.All incoming detrital pyrite was oxidized in the surface layers (0–10 cm) of the medium-high marsh overgrown withSpartina anglica. Pyrite was formed at a rate of 2.6–3.8 mol S-FeS₂m^–2yr^–1 in a narrow range of depths (15–20cm), at the interface of the oxidizing and underlying reducing sediment. At this interface the concentration profiles of Fe²⁺ and dissolved S intersected. The role of the rhizosphere is discussed in connection with pyrite formation. No further pyrite formation occurred deeper in the sediment. This resulted in the build up of high concentrations of dissolved S and acid volatile sulfides (AVS). The decrease with depth in oxalate-extractable Fe indicated that most of the iron oxyhydroxides (70–80%) had been transformed to pyrite. Another 10–20% of oxalate-extractable Fe was present as AVS. The abundance of framboidal pyrite particles and the high concentrations of AVS and dissolved S indicated that the formation of pyrite occurred via iron monosulfide intermediatesThere was a linear relationship between the organic carbon and the S-FeS₂ content in theSpartina overgrown reducing sediment. The mean C/S ratio was 4.2.