共查询到20条相似文献,搜索用时 0 毫秒
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Summary A simple and easily applicable method for determination of the ethanol fermentation rate is described. The formulas to be used for the ethanol productivity calculation in the case of free and agar immobilized cells are given. 相似文献
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A new enzymic method for inorganic phosphate determination 总被引:3,自引:0,他引:3
R K Scopes 《Analytical biochemistry》1972,49(1):88-94
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Donald D. Van Slyke 《The Biochemical journal》1938,32(9):1614-(1614)
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Mason MF 《The Biochemical journal》1938,32(4):719-724
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A new colorimetric method has been developed for the determination of creatine phosphokinase (CPK). This method is based on the reaction of creatine, formed enzymatically from creatine phosphate and ADP, with p-nitrophenylglyoxal (PNPG) under alkaline conditions to produce a colored product which absorbs maximally at 480 nm. At 25 degrees C the reaction was complete after 10 min in 0.1 M sodium pyrophosphate, pH 12, containing 0.15 M sodium ascorbate. The colored product was stable for at least 24 h and obeyed Beer's law in the range 0.005-0.05 mM creatine. The color reaction was used to determine the activity of CPK in serum and tissue extracts. The results obtained by this method agreed well with the results obtained by other available methods for CPK determination. However, the PNPG method was more rapid and more sensitive than other colorimetric methods and required a single chromogenic reagent. 相似文献
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Chowienczyk P. J.; Rees P. J.; Payne J.; Clark T. J. 《Journal of applied physiology》1981,50(3):672-678
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A new fluorometric method for RNA and DNA determination 总被引:40,自引:0,他引:40
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Pirie NW 《The Biochemical journal》1946,40(1):100-102
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A new micro method for determination of cholesterol in serum 总被引:6,自引:0,他引:6