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1.
A Gram-negative, strictly aerobic, motile, short rod-shaped bacterium with peritrichous flagella, designated JYr12T, was isolated from sediment of an amphioxus breeding zone in the coastal region of Qingdao, China. Strain JYr12T was found to grow optimally at 28 °C, at pH 7.0 and in the presence of 2–3 % (w/v) NaCl. The organism was determined to contain C16:0 (28.3 %) and C16:1 ω7c and/or C16:1 ω6c (34.8 %) as the major fatty acids and ubiquinone-8 (90 %) as the predominant isoprenoid ubiquinone. The polar lipids were found to consist of phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol and an unknown glycolipid. The G+C content of the genomic DNA was determined to be 42.3 mol %. Comparative 16S rRNA gene sequence analysis revealed that strain JYr12T was placed in the class Gammaproteobacteria and shared ~91.20, 91.18, 91.01, 90.95, 90.80 and 90.40 % sequence similarities with representatives of the genus Thalassolituus, Nitrincola, Oceanospirillum, Marinobacterium, Marinomonas and Microbulbifer, respectively. On the basis of phenotypic, chemotaxonomic and genotypic data, strain JYr12T is considered to represent a novel species in a new genus within the Gammaproteobacteria, for which the name Litoribacillus peritrichatus gen. nov., sp. nov. is proposed. The type strain of Litoribacillus peritrichatus is JYr12T (=CGMCC 1.10796T = JCM17551T).  相似文献   

2.
A taxonomic study was carried out on a novel aerobic bacterial strain (designated CC-LY736T) isolated from a fermentor in Taiwan. Cells of strain CC-LY736T were Gram-stain negative, spiral-shaped and motile by means of a monopolar flagellum. Strain CC-LY736T shared the greatest degree of 16S rRNA gene sequence similarity to Azospirillum irakense DSM 11586T (97.2 %), Rhodocista centenaria JCM 21060T (96.3 %) and Rhodocista pekingensis JCM 11669T (96.1 %). The major fatty acids were C16:0, C16:1 ω5c, C19:0 cyclo ω8c, C18:1 ω7c/C18:1 ω6c, C16:0 3-OH and C18:1 2-OH. The predominant polar lipids included phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidyldimethylethanolamine and two unidentified glycolipids. The common major respiratory quinone was ubiquinone Q-10 and predominant polyamines were sym-homospermidine and putrescine. The DNA G+C content of strain CC-LY736T was 67.6 ± 0.1 mol %. During phylogenetic analysis, strain CC-LY736T formed a unique phyletic lineage associated with Rhodocista species. However, the combination of genetic, chemotaxonomic and physiological data clearly indicated that strain CC-LY736T was a novel representative of the family Rhodospirillaceae. Based on the polyphasic comparison, the name Niveispirillum fermenti gen. nov., sp. nov. is proposed; the type strain of the type species is CC-LY736T (= BCRC 80504T = LMG 27263T). In addition, the reclassifications of Azospirillum irakense as Niveispirillum irakense comb. nov. (type strain KBC1T = ATCC 51182T = BCRC 15764T = CIP 103311T), and Azospirillum amazonense as Nitrospirillum amazonense gen. nov., sp. nov. (type strain Am14T = ATCC 35119T = BCRC 14279T = DSM 3787T) are proposed based on the polyphasic taxonomic data obtained in this study.  相似文献   

3.
An aerobic, Gram-negative, coccoid to short rod-shaped and non-flagellated marine bacterial strain S354T was isolated from seawater of Micronesia. The strain was capable to degrade agar-forming slight depression into agar plate. Growth occurred at a temperature range of 12–44 °C, a pH range of 5–9, and a salinity range of 1–7 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences suggested that S354T belongs to the family Flammeovirgaceae. The novel strain was most closely related to Limibacter armeniacum YM 11-185T with similarity of 92.5 %. The DNA G+C content was 43.8 mol%. The major fatty acids (>10 %) were iso-C15:0 and C16:1 ω5c. The predominant isoprenoid quinone was determined to be MK-7. Polar lipid profile of S354T consisted of phosphatidylethanolamine, unknown polar lipid, and unknown glycolipids. Based on the phenotypic, phylogenetic, biochemical, and physiological tests conducted in this study, S354T is proposed to represent a type strain of a novel genus and species. The 16S rRNA gene sequence of S354T is registered in GenBank under the accession number JQ639084. The type of strain Algivirga pacifica gen. nov., sp. nov. is S354T (=KCCM 90107T=JCM 18326T).  相似文献   

4.
A taxonomic study was carried out on strain 22II-S10r2T, which was isolated from the deep sea sediment of the Atlantic Ocean using oil-degrading enrichment. The bacterium was Gram-negative, oxidase positive and catalase negative, spherical in shape, and motile by polar flagella. Growth was observed at salinities of 0.5–7 % and at temperatures of 10–41 °C. The isolate was capable of aesculin hydrolysis, but unable to reduce nitrate to nitrite or degrade Tween 80 or gelatine. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 22II-S10r2T belonged to the family Ectothiorhodospiraceae, with highest sequence similarity to Thioalkalivibrio sulfidiphilus HL-EbGR7T (90.9 % similarity). The principal fatty acids were Sum In Feature 8 (C18:1 ω7c/ω6c (29.9 %), C18:1 ω9c (13.5 %), C16:1 ω5c (12.3 %), C12:03OH (6.8 %), C18:1 ω5c (5.7 %) and C16:0 (5.3 %). The G+C content of the chromosomal DNA was 60.7 mol%. The respiratory quinone was determined to be Q-7 (25 %) and Q-8 (75 %). Phosphatidylethanolamine, phosphatidylglycerol, aminophospholipid, glycolipid, three phospholipids and lipid were present. The strain was aerobic, non-phototrophic and non-chemolithoautotrophic. The combined genotypic and phenotypic data show that strain 22II-S10r2T represents a novel species within a novel genus, for which the name Maricoccus atlantica gen. nov. sp. nov. is proposed, with the type strain 22II-S10r2T (=CGMCC NO.1.12317T = LMG 27155T = MCCC 1A09384T).  相似文献   

5.
A taxonomic study was carried out on strain 22II-S10sT, which was isolated from the surface seawater of the Atlantic Ocean. The bacterium was found to be Gram-negative, oxidase and catalase positive, rod shaped and motile by subpolar flagella. The isolate was capable of gelatine hydrolysis but unable to reduce nitrate to nitrite or degrade Tween 80 or aesculin. Growth was observed at salinities of 0.5–18 % (optimum, 2–12 %), at pH of 3–10 (optimum, 7) and at temperatures of 10–41 °C (optimum 28 °C). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 22II-S10sT belongs to the genus Roseivivax, with highest sequence similarity to Roseivivax halodurans JCM 10272T (97.2 %), followed by Roseivivax isoporae LMG 25204T (97.0 %); other species of genus Roseivivax shared 95.2–96.7 % sequence similarity. The DNA–DNA hybridization estimate values between strain 22II-S10sT and the two type strains (R. halodurans JCM 10272T and R. isoporae LMG 25204T) were 22.00 and 21.40 %. The principal fatty acids were identified as Summed Feature 8 (C18:1 ω7c/ω6c) (67.4 %), C18:0 (7.2 %), C19:0 cyclo ω8c (7.1 %), C18:1 ω7c 11-methyl (6.8 %) and C16:0 (5.9 %). The respiratory quinone was determined to be Q-10 (100 %). Phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an aminolipid, a glycolipid and three phospholipids were present. The G+C content of the chromosomal DNA was determined to be 67.5 mol%. The combined genotypic and phenotypic data show that strain 22II-S10sT represents a novel species within the genus Roseivivax, for which the name Roseivivax atlanticus sp. nov. is proposed, with the type strain 22II-S10sT (= MCCC 1A09150T = LMG 27156T).  相似文献   

6.
A new bacterial strain, designated as FF42T, was isolated from feces of domestic pigs—collected from Suwon, Korea—and was characterized to determine its taxonomic position. Strain FF42T was observed to be Gram negative, aerobic, non-spore forming, motile, and rod-shaped cells. Based on the phylogenetic and 16S rRNA sequence analyses, it was revealed that strain FF42T belonged to the genus Comamonas. The highest degree of sequence similarities was determined to be with Comamonas zonglianii BF-3T (96.3 %), Comamonas composti CC-YY287T (96.1 %), and Comamonas nitrativorans 23310T (95.9 %), while showing less than 95.6 % identity with the remaining Comamonas species. Growth of strain FF42T occurred between 25 and 40 °C (optimum, 28 °C) and at pH of 5-9 (optimum, pH 6.0). It grew in the presence of 0–3 % (w/v) NaCl while minimally tolerating at 3 % (w/v) NaCl. Biochemical and physiological tests revealed phenotypic differentiation of strain FF42T to other members of the genus Comamonas. The predominant quinone is ubiquinone (Q-8). The major cellular fatty acids were C10:0 3OH, C16:0, summed feature 3 (C16:1 ω7c/C16:1 ω6c), and summed feature 8 (C18:1 ω6c/C18:1 ω7c), all of which have previously been reported to occur in the species of the genus Comamonas. The G+C molar content for strain FF42T is 60.2 mol %. Based on phylogenetic and phenotypic analyses, strain FF42T (=KEMC 1002-058T=JCM 17561T) is clearly referred to be a novel species for the genus Comamonas, for which the name Comamonas faecalis sp. nov. is proposed.  相似文献   

7.
A Gram-negative, non-motile, short rod-shaped bacterial strain, designated N5T, was isolated from a rice field soil in South Korea. Phylogenetic analysis based on the 16S rRNA gene sequence of the new isolate showed that strain N5T belongs to the genus Deinococcus, family Deinococcaceae, showing the highest sequence similarity to Deinococcus grandis KACC 11979T (98.4 %) and Deinococcus daejeonensis KCTC 13751T (97.5 %). Strain N5T exhibits resistance to gamma-radiation similar to that of other members of the genus Deinococcus, with a D10 value in excess of 4 kGy. Chemotaxonomic data showed that the most abundant fatty acids are C16:1 ω7c (25.25 %), C15:1 ω6c (19.77 %), C17:1 ω6c (11.87 %), and C17:0 (9.41 %), and the major polar lipid is an unknown phosphoglycolipid. The predominant respiratory quinone is menaquinone MK-8. The DNA G+C content is 71.4 mol%. Phenotypic, phylogenetic, and chemotaxonomic data support designation of strain N5T as a novel species of the genus Deinococcus, for which the name Deinococcus soli sp. nov. is proposed. The type strain is N5T (=KCTC 33153T = JCM 19176T).  相似文献   

8.
A novel bacterial strain designated GJW-30T was isolated from soil of the lava forest, Gotjawal, located in Aewol, Jeju, Korea. Strain GJW-30T was found to be strictly aerobic, Gram-negative and to form pleomorphic, non-motile rods and white colonies on R2A agar. The major fatty acids were identified as C18:1ω7c, C16:0 and C17:0, the predominant isoprenoid quinone as Q-10, the polar lipids as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified aminolipid and an unidentified lipid. The cell-wall sugar pattern of strain GJW-30T was found to be composed of glucose, ribose and rhamnose and meso-DAP as the diagnostic diamino acid in the cell-wall peptidoglycan. The DNA G+C content of strain GJW-30T is 62.2 mol%. Phylogenetic analysis, based on 16S rRNA gene sequence similarities, showed that strain GJW-30T forms a deep branch within the order Rhizobiales, sharing the highest level of sequence homology with Bradyrhizobium oligotrophicum LMG 10732T (93.6 %). On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, strain GJW-30T is considered to represent a novel genus and species, for which the name Variibacter gotjawalensis gen. nov., sp. nov. (the type strain is GJW-30T = KCTC 32391T = CECT 8514T = LMG 28093T) is proposed.  相似文献   

9.
A Gram-negative, aerobic, motile rod strain, designated Ma-20T, was isolated from a pool of marine Spirulina platensis cultivation, Sanya, China, and was subjected to a polyphasic taxonomy study. Strain Ma-20T can grow in the presence of 0.5–11 % (w/v) NaCl, 10–43 °C and pH 6–10, and grew optimally at 30 °C, pH 7.5–9.0 in natural seawater medium. The polar lipids were composed of phosphatidylethanolamine, three unidentified phospholipids and three unidentified polar lipids. The respiratory quinone was ubiquinone 8 (Q-8) and the major fatty acids were C18:1ω6c/C18:1ω7c (summed feature 8, 32.84 %), C16:1ω6c/C16:1ω7c (summed feature 3, 30.76 %), C16:0 (13.54 %), C12:03-OH (4.63 %), and C12:0 (4.09 %). The DNA G+C content of strain Ma-20T was 58 mol %. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Ma-20T belonging to Gammaproteobacteria, it shared 88.46–91.55 and 89.21–91.26 % 16S rRNA gene sequence similarity to the type strains in genus Hahella and Marinobacter, respectively. In addition to the large 16S rRNA gene sequence difference, Ma-20T can also be distinguished from the reference type strains Hahella ganghwensis FR1050T and Marinobacter hydrocarbonoclasticus sp. 17T by several phenotypic characteristics and chemotaxonomic properties. On the basis of phenotypic, chemotaxonomic and phylogenetic properties, strain Ma-20T is suggested to represent a novel species of a new genus in Gammaproteobacteria, for which the name Nonhongiella spirulinensis gen. nov., sp. nov. is proposed. The type strain is Ma-20T (=KCTC 32221T=LMG 27470T).  相似文献   

10.
A novel strain, DCY108T was isolated from soil of a Panax ginseng field, Yeoncheon province (38°04′N 126°57′E), Republic of Korea. Strain DCY108T is Gram-negative, non-motile, non-flagellate, rod-shaped, and aerobic. The bacterium grows optimally at 25–30 °C, pH 6.5–7.0 and 1 % NaCl. Phylogenetically, strain DCY108T is closely related to Pedobacter jejuensis JCM 18824T, Pedobacter aquatilis JCM 13454T, Pedobacter kyungheensis LMG 26577T and the type strain of the genus Pedobacter heparinus DSM 2366T. The DNA–DNA relatedness values between strain DCY108T and its close phylogenetic neighbors were below 30.0 %. The DNA G+C content of strain DCY108T was determined to be 45.1 mol%. The predominant quinone was menaquinone 7 (MK-7). The major polar lipids were identified as phosphatidylethanolamine and three unidentified aminolipids AL1, AL13 and AL17. Iso-C15:00, iso-C17:03OH and summed feature 3 (C16:1 ω7c/C16:1 ω6c) were identified as the major fatty acids present in strain DCY108T. The results of physiological and biochemical tests allowed strain DCY108T to be differentiated phenotypically from other recognized species belonging to the genus Pedobacter. Therefore, it is suggested that the newly isolated organism represents a novel species, for which the name Pedobacter panacis sp. nov is proposed with the type strain designated as DCY108T (=CCTCCAB 2015196T = KCTC 42748T).  相似文献   

11.
A novel marine, Gram-negative, rod-shaped bacterium, designated strain N9T, was isolated from a water sample of the sea shore at Visakhapatnam, Andhra Pradesh (India). Strain N9T was found to be positive for oxidase and catalase activities. The fatty acids were found to be dominated by C16:0, C18:1 ω7c and summed in feature 3 (C16:1 ω7c and/or C16:1 ω6c). Strain N9T was determined to contain Q-10 as the major respiratory quinone and phosphatidylethanolamine, phosphatidylglycerol, two aminophospholipids, two phospholipids and four unidentified lipids as polar lipids. The DNA G+C content of the strain N9T was found to be 63 mol%. 16S rRNA gene sequence analysis indicated that Rhodobacter sphaeroides, Rhodobacter johrii, Pseudorhodobacter ferrugineus, Rhodobacter azotoformans, Rhodobacter ovatus and Pseudorhodobacter aquimaris were the nearest phylogenetic neighbours, with pair-wise sequence similarities of 95.43, 95.36, 94.24, 95.31, 95.60 and 94.74 %, respectively. Phylogenetic analysis showed that strain N9T formed a distinct branch within the family Rhodobacteraceae and clustered with the clade comprising species of the genus Pseudorhodobacter, together with species of the genera Roseicitreum, Roseinatronobacter, Roseibaca and Rhodobaca. Species of the genus Pseudorhodobacter are phylogenetically close with a 16S rRNA gene sequence dissimilarity of 5.9–7.3 % (92.7–94.1 % similarity). Based on the above-mentioned phenotypic characteristics and on phylogenetic inference, strain N9T is proposed as a representative of a new genus and a novel species of the family Rhodobacteraceae as Albirhodobacter marinus gen. nov., sp. nov. The type strain of Albirhodobacter marinus is N9 (= MTCC 11277T = JCM 17680T).  相似文献   

12.
The strain designated as AB21T was isolated from chloroethylenes contaminated soil. Cells are gram-negative, aerobic, non-spore-forming, and motile rods. Phylogenetic analysis based on 16S rRNA gene sequence showed that it belonged to the genus Rhizobium, and was closely related to Rhizobium sullae IS 123T (97.4 %), Rhizobium yanglingense SH 22623T (97.2 %), Rhizobium gallicum R 602spT (97.1 %), Rhizobium alamii GBV 016T (97.0 %), and Rhizobium monogolense USDA 1844T (97.0 %). It showed less than 97 % identity with the remaining Rhizobium species. This novel isolate grew optimally at 25–37 °C (optimum, 30 °C) and pH 6–9 (optimum, pH 8.0). It grew in the presence of 0–4 % (w/v) NaCl, tolerating a 4 % (w/v) NaCl. DNA–DNA hybridization experiment shows less than 53 % binding with closely related Rhizobium. Predominant quinone is ubiquinone (Q-10). The major fatty acids were summed feature 8 (composed of C18:1 ω7c/C18:1 ω6c), C19:0 cyclo ω8c, and C16:0. The G+C molar content is 62.5 mol%. Based on the polyphasic analysis, strain AB21T is referred to be a novel species of the genus Rhizobium for which the name Rhizobium halotolerans sp. nov. is proposed. The type strain is AB21T (=KEMC 224-056T = JCM 17536T).  相似文献   

13.
A strain designated as S85T was isolated from a seaweed collected from coastal area of Chuuk State in Micronesia. The strain was gram-negative, rod-shaped, and non-motile and formed yellow colonies on the SWY agar (0.2 % yeast extract and 1.5 % agar in seawater) and Marine agar 2216. The strain grew at pH 5–9 (optimum, pH 8), at 15–40 °C (optimum, 25–28 °C), and with 1–9 % (w/v) NaCl (optimum, 3 %). The phylogenetic analysis based on 16S rRNA gene sequence showed that strain S85T was related to Lutibacter litoralis CL-TF09T and Maritimimonas rapanae A31T with 91.4 % and with 90.5 % similarity, respectively. The dominant fatty acids were iso-C15:0, iso-C15:0 3-OH and iso-C17:0 3-OH, C16:0 3-OH and summed feature 3 (C16:1 ω7c and/or iso-C15:0 2-OH). The major isoprenoid quinone was MK-6. The DNA G+C content of the type strain was 34.6 mol %. The major polar lipids were phosphatidylethanolamine, an unknown glycolipid and two unknown polar lipids. Based on this polyphasic taxonomic data, strain S85T stands for a novel species of a new genus, and we propose the name Ochrovirga pacifica gen. nov., sp. nov. The type strain of O. pacifica is S85T (=KCCM 90106 =JCM 18327T).  相似文献   

14.
A Gram stain-negative, aerobic and rod-shaped bacterium, strain DY22T, was isolated from a deep-sea sediment collected from the east Pacific Ocean. The isolate was found to grow in the presence of 0–20.0 % (w/v) NaCl and at pH 4.5–8.5; optimum growth was observed with 0.5–2.0 % (w/v) NaCl and at pH 5.0–7.0. Chemotaxonomic analysis showed the presence of ubiquinone-9 as predominant respiratory quinone and C16:0, C19:0 ω8c cyclo and C12:0 3-OH as major cellular fatty acids. The genomic DNA G+C content was determined to be 59.6 mol%. Comparative 16S rRNA gene sequence analysis revealed that the novel isolate belongs to the genus Salinicola. Strain DY22T exhibited the closest phylogenetic affinity to the type strain of Salinicola salarius with 97.2 % sequence similarity and less than 97 % sequence similarity with respect to other Salinicola species with validly published names. The DNA–DNA reassociation values between strain DY22T and S. salarius DSM 18044T was 52 ± 4 %. On the basis of phenotypic, chemotaxonomic and genotypic data, strain DY22T represents a novel species of the genus Salinicola, for which the name Salinicola peritrichatus sp. nov. (type strain DY22T = CGMCC 1.12381T = JCM 18795T) is proposed.  相似文献   

15.
A new aerobic, Gram-negative, chemo-organotrophic, euryhaline bacterium, designated strain JL2009T, was isolated from surface water of the Caribbean Sea. The strain formed flaxen colonies on marine ager 2216 (MA; Difco) medium. Cells were dimorphic, with stalks or a polar flagellum, and reproduction occurred by means of binary fission. Growth occurred at 15–45 °C (optimum at 35 °C), 0–15 % (w/v) NaCl (optimum at 1–10 %) and pH 5–9 (optimum at pH 7–8). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a distinct evolutionary lineage within the family Hyphomonadaceae. Strain JL2009T was most closely related to Maricaulis parjimensis MCS 25T (92.2 % DNA sequence similarity), Woodsholea maritime CM2243T (90.9 %), and Oceanicaulis alexandrii C116-18T (90.9 %). The major respiratory quinone was Q-10. The dominant cellular fatty acids were summed feature 8 (C18:1 ω7c), C18:0 and 11-methyl C18:1 ω7c. The polar lipid pattern indicated the presence of phospholipid, phosphatidyl glycerol and glycolipids. The G + C content of the genomic DNA was 70.5 mol%. On the basis of the chemotaxonomic and phenotypic characteristics and the phylogenetic evidence, strain JL2009T represents a novel genus and species in the family Hyphomonadaceae, for which the name Euryhalocaulis caribicus gen. nov., sp. nov. is proposed. The type strain of Euryhalocaulis caribicus is JL2009T (=CGMCC 1.12036T = JCM 18163T).  相似文献   

16.
Two deltaproteobacterial sulfate reducers, designated strain I.8.1T and I.9.1T, were isolated from the oxygen minimum zone water column off the coast of Peru at 400 and 500 m water depth. The strains were Gram-negative, vibrio-shaped and motile. Both strains were psychrotolerant, grew optimally at 20°C at pH 7.0–8.0 and at 2.5–3.5% NaCl (w/v). The strains grew by utilizing hydrogen/acetate, C3–4 fatty acids, amino acids and glycerol as electron acceptors for sulfate reduction. Fumarate, lactate and pyruvate supported fermentative growth. Sulfate, sulfite, thiosulfate and taurin supported growth as electron acceptors. Both strains were catalase-positive and highly oxygen-tolerant, surviving 24 days of exposure to atmospheric concentrations. MK6 was the only respiratory quinone. The most prominent cellular fatty acid was iso-17:1-ω9c (18%) for strain I.8.1T and iso-17:0-ω9c (14%) for strain I.9.1T. The G+C contents of their genomic DNA were 45–46 mol%. Phylogenetic analysis of 16S rRNA and dsrAB gene sequences showed that both strains belong to the genus Desulfovibrio. Desulfovibrio acrylicus DSM 10141T and Desulfovibrio marinisediminis JCM 14577T represented their closest validly described relatives with pairwise 16S rRNA gene sequence identities of 98–99%. The level of DNA-DNA hybridization between strains I.8.1T and I.9.1T was 30–38%. The two strains shared 10–26% DNA-DNA relatedness with D. acrylicus. Based on a polyphasic investigation it is proposed that strains I.8.1T and I.9.1T represent a novel species for which the name Desulfovibrio oceani sp. nov. is proposed with the two subspecies D. oceani subsp. oceani (type strain, I.8.1T = DSM 21390T = JCM 15970T) and D. oceani subsp. galateae (type strain, I.9.1T = DSM 21391T = JCM 15971T).  相似文献   

17.
A Gram stain-negative, yellowish green-pigmented, facultatively anaerobic, motile, curved rod-shaped bacterium designated as strain JJ016T was isolated from an artificial lake in South Korea, and characterized using a polyphasic approach. The 16S rRNA gene sequence of strain JJ016T indicated that the isolate belonged to the family Rhodocyclaceae and exhibited 95.0% identity to Uliginosibacterium gangwonense 5YN10-9T. The major cellular fatty acids of the novel strain were summed feature 3 (C16:1 ω6c and/or C16:1 ω7c), C16:0, C14:0, and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c). The DNA G+C content of strain JJ016T was 61.9 mol%. The major respiratory quinone and major polar lipid of strain JJ016T were ubiquinone-8 and phosphatidylethanolamine, respectively. Based on the morphological and physiological properties and the biochemical evidence presented, we concluded that strain JJ016T represents a novel species of a new genus in the family Rhodocyclaceae, for which the name Viridibacterium curvum gen. nov., sp. nov. is proposed. The type strain is JJ016T (=KACC 16899T =JCM 18715T).  相似文献   

18.
A bacterial strain, designated M26T, was isolated from a fish gastrointestinal tract, collected from Zhanjiang Port, South China. 16S rRNA gene sequence analysis indicated that strain M26T belongs to the subclass α-Proteobacteria, being related to the genus Paracoccus, and sharing highest sequence similarity with Paracoccus alcaliphilus JCM 7364T (98.1 %), Paracoccus huijuniae FLN-7T (97.3 %), Paracoccus stylophorae KTW-16T (97.1 %) and Paracoccus seriniphilus DSM 14827T (96.9 %). The major quinone was determined to be ubiquinone Q-10, with Q-9 and Q-8 as minor components. The major fatty acid was identified as C18:1ω7c, with smaller amounts of C18:0 and C16:0. The G+C content of the genomic DNA was determined to be 64.3 mol%. The DNA hybridization value between strain M26T and the most closely related type strain, P. alcaliphilus, was 29.0 ± 1.0 %. The results of physiological and biochemical tests and low DNA–DNA relatedness showed that the strain could be readily distinguished from closely related species. On the basis of these phenotypic and genotypic data, strain M26T is concluded to represent a novel species of the genus Paracoccus, for which the name Paracoccus siganidrum sp. nov. is proposed. The type strain is M26T (=CCTCC AB 2012865T = DSM 26381T).  相似文献   

19.
A Gram-negative, strictly aerobic, non-motile, non-spore-forming and rod-shaped bacterial strain designated KHI67T was isolated from sediment of the Gapcheon River in South Korea and its taxonomic position was investigated by using a polyphasic approach. Strain KHI67T was observed to grow optimally at 25–30 °C and at pH 7.0 on nutrient and R2A agar. On the basis of 16S rRNA gene sequence similarity, strain KHI67T was shown to belong to the family Sphingomonadaceae and was related to Sphingomonas faeni MA-olkiT (97.6 % sequence similarity), Sphingomonas aerolata NW12T (97.5 %) and Sphingomonas aurantiaca MA101bT (97.3 %). The G + C content of the genomic DNA was determined to be 65.6 %. The major ubiquinone was found to be Q-10, the major polyamine was identified as homospermidine and the major fatty acids identified were summed feature 8 (comprising C18:1 ω7c/ω6c; 37.0 %), C16:0 (13.0 %), summed feature 3 (comprising C16:1 ω7c/C16:1 ω6c; 12.8 %) and C14:0 2OH (9.3 %). DNA and chemotaxonomic data supported the affiliation of strain KHI67T to the genus Sphingomonas. The DNA–DNA relatedness values between strain KHI67T and its closest phylogenetic neighbours were below 15 %. Strain KHI67T could be differentiated genotypically and phenotypically from the recognised species of the genus Sphingomonas. The isolate therefore represents a novel species, for which the name Sphingomonas ginsenosidivorax sp. nov. is proposed, with the type strain KHI67T (=KACC 14951T = JCM 17076T = LMG 25801T).  相似文献   

20.
A Gram-staining-negative, rod-shaped and motile with several polar flagellums bacterium, designated WM-3T, was isolated from a rice paddy soil in South China. Growth occurred with 0–3.0 % (w/v) NaCl (optimum 2.0 %), at pH 5.5–9.0 (optimum pH 7.0) and at 25–42 °C (optimum 30–37 °C) in liquid Reasoner’s 2A medium. Analysis of the 16S rRNA gene and gyrB gene sequences revealed that strain WM-3T was most closely related to the type strains of the species Pseudomonas linyingensis and Pseudomonas sagittaria. Its sequence similarities with P. linyingensis CGMCC 1.10701T and P. sagittaria JCM 18195T were 97.4 and 97.3 %, respectively, for 16S rRNA gene, and were 94.1 and 94.2 %, respectively, for gyrB gene. DNA–DNA hybridization between strain WM-3T and these two type strains showed relatedness of 35.6 and 30.9 %, respectively. G+C content of genomic DNA was 69.4 mol%. The whole-cell fatty acids mainly consisted of C16:0 (30.0 %), C16:1 ω6c and/or C16:1 ω7c (19.3 %) and C18:1 ω6c and/or C18:1 ω7c (16.3 %). The results of phenotypic, chemotaxonomic and genotypic analyses clearly indicated that strain WM-3T belongs to genus Pseudomonas but represents a novel species, for which the name Pseudomonas oryzae sp. nov. is proposed. The type strain is WM-3T (=KCTC 32247T =CGMCC 1.12417T).  相似文献   

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