Spatial distribution patterns of calcareous nannofossils across the Callovian–Oxfordian transition in the French Subalpine Basin

Callovian–Oxfordian calcareous nannofossils are documented from four sections distributed along a proximal–distal (slope to central basin) transect in the French Subalpine Basin (south-eastern France). The sections were dated and correlated using integrated ammonite, dinoflagellate and nannofossil biostratigraphies. The nannofossil assemblages are constantly dominated by Watznaueria britannica. However, significant spatial changes in nannofossil assemblage composition are recorded. Nannofossil assemblages recovered in slope and slope-to-basin transition sections are characterized by high abundances, lowest diversities, highest percentages of smallest-sized morphotypes of W. britannica, subordinated W. britannica/manivitiae (a morphotype described in this study), and are indicative of turbulent, unstable and/or eutrophic conditions. Towards the central part of the basin, as the nutrient content and turbulence declined and paleoenvironmental stability increased, the nannofossil assemblages become more diverse and characterized by lower relative abundances of W. britannica but higher contribution of larger morphotypes and higher relative abundances of W. barnesiae/fossacincta, Biscutum dubium and Zeugrhabdotus erectus. These latter small coccoliths, markers of high surface-waters fertility for the Cretaceous, were probably not competitive with respect to smaller-sized W. britannica in unstable and eutrophic surface waters for the Jurassic. An increase in the surface water productivity is recorded both in proximal and distal sections at the Callovian–Oxfordian transition, and is demonstrated both by an increase in nannofossil total abundances and in the relative abundances of taxa adapted to high-trophic levels. Thermal minimum in surface waters, as indicated by an increase in the percentages of the cool-water nannofossil taxa, is associated to increased productivity. Further studies will be necessary to demonstrate whether surface-water productivity changes, already recorded in the eastern Paris Basin, were global.     

Size analyses of the coccolith species Biscutum constans and Watznaueria barnesiae from the Late Albian “Niveau Breistroffer” (SE France): taxonomic and palaeoecological implications

The size variability of the coccolith species Biscutum constans and Watznaueria barnesiae has been studied in 50 samples of the Late Albian “Niveau Breistroffer” black shales (Col de Palluel section, SE France). For each species, length and width of the total coccolith and the central unit have been measured in 60 specimens per sample. In addition, ellipticity and central unit length/total coccolith length ratio have been calculated. This study aims to improve our understanding of the taxonomic concepts of B. constans and W. barnesiae, and to test whether the size changes correspond to palaeoceanographic changes interpreted from other proxies. Two morphotypes (varieties) were differentiated for each of the two taxa studied. B. constans includes morphotypes with a narrow (B. constans var. constans) and a wide central unit (B. constans var. ellipticum). Between these two morphotypes no significant size differentiation has been observed. They seem to represent end members of a size continuum, relating to both the total coccolith- and central unit size distribution. The two morphotypes of W. barnesiae differ only in the absence (W. barnesiae var. barnesiae) and the presence of a narrow central opening (W. barnesiae var. fossacincta). No significant size differences have been observed between these two morphotypes, and both show similar distributions of the measured characteristics. We recommend that the studied morphotypes of both B. constans and W. barnesiae should not be assigned to different morphospecies. Throughout the studied black shale succession the measured parameters show mostly statistically insignificant, short-term fluctuations, but significant long-term trends have been observed for B. constans. These show a trend to smaller forms with a narrower central unit in the upper part of the succession. This change coincides with a cooling trend, as indicated by a nannofossil based temperature index. Therefore the two morphotypes of B. constans are interpreted to represent ecophenotypic varieties rather than two different morphospecies. No clear relationship has been recognized between the size of W. barnesiae and the palaeoenvironmental conditions.     

Morphologic variability of the coccolithophorid Calcidiscus leptoporus in the plankton, surface sediments and from the Early Pleistocene

On a global scale, morphological variability of the extant coccolithophorid Calcidiscus leptoporus (Murray and Blackman, 1898) Loeblich and Tappan was investigated in surface sediments and plankton samples and from an Early Pleistocene time-slice (1.8 Ma to 1.6 Ma). In the bivariate space coccolith diameter versus number of rays in the distal shield, Holocene samples follow a single, unimodal morphocline. Sample means of coccolith size and number of elements group in three clusters, I, II and III, which are of biogeographic significance. Clusters II and III coccoliths (mean coccolith size of 5.0 μm and 20.9 elements, and 6.6 μm and 25.6 elements, respectively) are found in a tropical belt extending from 11 °N to 17 °S with an annual minimum sea-surface temperature above 23.5 °C. Cluster I coccoliths (5.8 μm, 20.7 elements) are found in samples outside that belt. The distribution of coccoliths in the surface sediments is tentatively interpreted to be a result of mixing to a varying degree of at least three different morphotypes (‘small’, ‘intermediate’ and ‘large’), which were identified in the living plankton, and which are separated from each other at 5 μm and 8 μm mean coccolith diameter, respectively. A comparison of the surface sediments with the Early Pleistocene assemblages revealed that between 1.6 Ma and 1.8 Ma two morphoclines A and B existed, the first of which persisted until the Holocene in the form of C. leptoporus, while the second comprises only extinct morphotypes including Calcidiscus macintyrei as one end-member. During the Early Pleistocene morphocline A was more homogeneous and no clusters were evident.Morphocline B shows a clear bimodality with a separation of morphotypes at 9.5 μm. Our observations suggest that morphoclines are subsets within the total stratigraphical range of a single species, and represent the global variability of that species in a particular time interval. Morphotypes, which belong to a morphocline, represent the infra-specific variability of that species within the biogeographic and stratigraphic limits of that species.     

Calcareous nannofossil productivity and carbonate production across the Middle-Late Jurassic transition in the French Subalpine Basin

The distribution pattern of calcareous nannofossils was analysed across the Middle-Late Jurassic transition in the French Subalpine Basin (south-eastern France). This basin is characterized in the hemipelagic-pelagic domain by a continuous sedimentary succession, allowing a good biostratigraphic resolution for this time interval. The nannofossil assemblages are consistently dominated by Watznaueria britannica. However, major changes in trophic and paleoenvironmental conditions are recorded across the Middle-Late Jurassic transition. An increase in marine primary productivity and cooling of surface waters is recorded across the Callovian-Oxfordian boundary, as already shown in the higher latitude setting of the eastern Paris Basin. Increased precipitation and runoff under contrasting seasonal climatic conditions (monsoon-type) has led to eutrophication of marine surface waters in the French Subalpine Basin at this period. Then, decreased runoff and associated nutrients certainly linked to drier climatic conditions lead to a decrease in calcareous nannofossil productivity during the middle part of the Early Oxfordian (mariae-cordatum ammonite Zone transition). At the Early-Middle Oxfordian transition, more favourable conditions for the nannofossil community (warmer and mesotrophic surface waters) prevailed. The pelagic (nannofossil) carbonate contribution is limited, and the carbonate fraction is predominantly of nektonic/benthic origin at the Callovian-Oxfordian transition and of allochthonous origin from carbonate platforms at the Early Oxfordian-Middle Oxfordian transition.     

Seasonal variation in Emiliania huxleyi coccolith morphology and calcification in the Aegean Sea (Eastern Mediterranean)

A seasonal morphological variability is observed in Emiliania huxleyi var. huxleyi specimens, collected from discrete water samples in the Aegean Sea. Biometric analyses reveal a consistent pattern of increase in the size of coccoliths and coccospheres, including the thickness of the inner tube elements (INT), in winter/spring time low sea surface temperature and moderate productivity samples when compared with summer time high temperature-low productivity samples. The small range of salinity change in the Aegean Sea and the absence of seasonal pattern in nutrient content do not support any association with the observed increase in E. huxleyi coccolith size. A relatively increased [HCO₃^-] content is observed during spring-time interval related with the increase in the coccolith size, however it remains unclear which parameter of the carbonate system causes the observed effects.     

Evolution of the coccolith genus Lotharingius during the Late Pliensbachian-Early Toarcian interval in Asturias (N Spain). Consequences of the Early Toarcian environmental perturbations

The evolution of the coccolith genus Lotharingius was investigated in 18 samples from the West Rodiles section (Asturias, Northern Spain), Upper Pliensbachian-Lower Toarcian in age. In each sample, the length and width of the coccolith and of its central area were measured on 150 specimens of Lotharingius. A total of 2700 specimens were measured, and 10,800 measurements were obtained. Based on the results of the morphometric analysis, the stratigraphic ranges, the abundance patterns and the light microscope observations, the aims of this work are to determine whether the specimens analysed correspond to different species, to distinct ecophenotypes or to an anagenetic lineage of morphotypes of a single species with overlapping stratigraphic ranges, and to test the influence of the paleoenvironmental perturbations during the Early Toarcian on the studied genus. Three species of Lotharingius were distinguished: L. hauffii, L. sigillatus and L. crucicentralis. Based on central area size and structure, two morphotypes (A and B) of L. hauffii were differentiated. All the parameters measured show a great overlap between the morphotypes and species. An increase in both coccolith and central area size and a development of more complex central area structures were observed through the studied section. L. hauffii is the smallest form, followed by L. sigillatus, and finally L. crucicentralis is the largest species and has the most complex central area structure. A clear decrease in size or “dwarfing” of the species was observed in the samples corresponding to the Early Toarcian environmental perturbations. L. crucicentralis was not identified in these samples. We hypothesize that the changes in size and abundance of the Lotharingius species could be related to the unfavourable palaeoenvironmental conditions for the biomineralization of their coccoliths.     

Calcareous nannoplankton in surface sediments of the East China Sea

Twenty-eight species of coccoliths were identified in surface sediments of the East China Sea. The species composition of the coccolith assemblages is similar to that of the North Pacific Water Mass, but differs from the latter by a greater dominance ofEmiliania huxleyi andGephyrocapsa oceanica (together making up over 90% specimens). The coccolith species composition shows considerable differences between the continental shelf and the Okinawa Trough areas, reflecting different water masses. On the other hand, the coccolith content in sediments is apparently controlled largely by the deposition rate of terrigenous material.     

Miocene variability of Calcidiscus gr. leptoporus and possible evolutionary relationship with another Coccolithaceae: Umbilicosphaera gr. sibogae.

Bibliographic data concerning the variability of coccosphere and coccoliths during the life-cycle of two extant Coccolithophorid species (Calcidiscus gr. leptoporus and Umbilicosphaera gr. sibogae) are summarized and completed by new observations on the structure of the proximal shield by fossil coccoliths. Thorough investigation of the proximal shield of C. gr. leptoporus in the latest Miocene (Messinian) assemblages from three oceanic localities (central Pacific, eastern equatorial Atlantic and southwestern Indian oceans) indicates the co-occurrence of two types of coccoliths: (1) typical C. gr. leptoporus and (2) specimens constituted by a distal shield similar to that of C. leptoporus, but with a proximal shield comparable to that of U. sibogae, although no typical U. sibogae occurs on the basis of the distal views. Such intermediate coccoliths have been previously described from the Badenian (middle Miocene) of central Europe. Whereas the authors considered them as a new species (Cycloperfolithus carlae), they are interpreted here as transitional morphotypes revealing an evolutionary link between C. gr. leptoporus and U. gr. sibogae, during the late Miocene. Consequently, the palaeontological 'species' carlae is hereby transferred to the genus Calcidiscus. These observations also point out the possibility of a diachronous evolution of the two shields of the same coccolith type, underlining the necessity for a better knowledge of the proximal shield structure, usually somewhat neglected.     

A GENETIC MARKER TO SEPARATE EMILIANIA HUXLEYI (PRYMNESIOPHYCEAE) MORPHOTYPES1

Emiliania huxleyi (Lohm.) Hay and Mohler is a ubiquitous unicellular marine alga surrounded by an elaborate covering of calcite platelets called coccoliths. It is an important primary producer involved in oceanic biogeochemistry and climate regulation. Currently, E. huxleyi is separated into five morphotypes based on morphometric, physiological, biochemical, and immunological differences. However, a genetic marker has yet to be found to characterize these morphotypes. With the use of sequence analysis and denaturing gradient gel electrophoresis, we discovered a genetic marker that correlates significantly with the separation of the most widely recognized A and B morphotypes. Furthermore, we reveal that the A morphotype is composed of a number of distinct genotypes. This marker lies within the 3′ untranslated region of a coccolith associated protein mRNA, which is implicated in regulating coccolith calcification. Consequently, we tentatively termed this marker the coccolith morphology motif.     

Calcareous nannofossil changes during the late Callovian–early Oxfordian cooling phase

Calcareous nannofossil quantitative and biostratigraphic analyses integrated with carbon and oxygen stable isotopes were carried out on the core ANDRA (Agence Nationale pour la gestion des Déchets Radio-Actifs—FRANCE) HTM 102 across the Callovian/Oxfordian boundary drilled at Cirfontaines-en-Ornois, Départment de Haute-Marne, eastern France. Calcareous nannofossil assemblages at the Callovian–Oxfordian transition are dominated by the genus Watznaueria. An increase in abundance of Biscutum spp. and A-group, which consists of Axopodorhabdus spp. (A. atavus, A. rahla, and A. cylindratus), Podorhabdus grassei, Octopodorhabdus decussatus, Hexapodorhabdus cuvillieri (family Axopodorhabdaceae), and Triscutum spp., correlates with a significant positive excursion in δ¹⁸O suggesting that these groups were probably adapted to cooler surface waters. A positive increase in δ¹³C values is coupled with high abundances of eutrophic taxa such as Zeughrabdotus erectus, Biscutum spp., and small-sized Watznaueria britannica, and a decrease in abundance of the big and oligotrophic taxa Schizosphaerella punctulata and Watznaueria manivitae. Climate cooling across the Callovian/Oxfordian boundary probably triggered a breakdown in stratification of surface waters leading to more intense nutrient recycling and higher primary productivity that favoured the shift in abundance of small-sized eutrophic taxa in the East Paris Basin.     

Biometry of Upper Cretaceous (Cenomanian–Maastrichtian) coccoliths – a record of long-term stability and interspecies size shifts

Biometric measurements of Mesozoic coccoliths (coccolith length and width) have been used in short-term biostratigraphic, taxonomic and palaeoecologic studies, but until now, not over longer time scales. Here, we present a long time-series study (∼ 30 million years) for the Upper Cretaceous, which aims to identify broad trends in coccolith size and to understand the factors governing coccolith size change over long time scales. We have generated biometric data for the dominant Upper Cretaceous coccolith groups, Broinsonia/Arkhangelskiella, Prediscosphaera, Retecapsa and Watznaueria, from 36 Cenomanian–Maastrichtian (100.5–66 Ma) samples from Goban Spur in the northeast Atlantic (DSDP Site 549). These data show that the coccolith sizes within Prediscosphaera, Retecapsa and Watznaueria were relatively stable through the Late Cretaceous, with mean size variation less than 0.7 μm. Within the Broinsonia/Arkhangelskiella group there was more pronounced variation, with a mean size increase from ∼ 6 μm in the Cenomanian to ∼ 10 μm in the Campanian. This significant change in mean size was largely driven by evolutionary turnover (species origination and extinctions), and, in particular, the appearance of larger species/subspecies (Broinsonia parca parca, Broinsonia parca constricta, Arkhangelskiella cymbiformis) in the early Campanian, replacing smaller species, such as Broinsonia signata and Broinsonia enormis. Shorter-term size fluctuations within Broinsonia/Arkhangelskiella, observed across the Late Cenomanian–Turonian and Late Campanian–Maastrichtian intervals, may, however, reflect changing palaeoenvironmental conditions, such as sea surface temperature and nutrient availability.     

An experimental study on post-mortem dissolution and overgrowth processes affecting coccolith assemblages: A rapid and complex process

Coccolith dissolution together with post-mortem morphological features are immensely important phenomena that can affect assemblage compositions, complicate taxonomic identification as well as provide valuable palaeoenvironmental insights. This study summarizes the effects of pH oscillations on post-mortem coccolith morphologies and the abundances and compositions of calcareous nannoplankton assemblages in three distinct types of material—(i) Cretaceous chalk, (ii) Miocene marls, and (iii) late Holocene calcareous ooze. Two independent experimental runs within a semi-enclosed system setting were realized to observe assemblage alterations. One experiment was realized with the presence of bacteria and, in contrast, the second one inhibited their potential effect on the studied system. The pH was gradually decreased within the range of 8.3–6.4 using a reaction of CO₂ with H₂O forming weak carbonic acid (H₂CO₃), thereby affecting [${\mathrm{CO}}_3^{2-}$]. Further, a subsequent overgrowth study was carried out during spontaneous degassing accompanied by a gradual pH rise. The experiment revealed that the process and intensity of coccolith corrosion and subsequent overgrowth build-ups are influenced by a plethora of different factors such as (i) pH and associated seawater chemistry, (ii) mineral composition of the sediment, (iii) the presence of coccoliths within a protective substrate (faecal pellets, pores, pits), and (iv) the presence/absence of bacteria. Nannoplankton assemblages with corroded coccoliths or with coccoliths with overgrowth build-ups showed that the observed relative abundances of taxa experienced alteration from the original compositions. Additionally, extreme pH oscillations may result in enhanced morphological changes that make coccoliths unidentifiable structures, and might even evoke the absence of coccoliths in the fossil record.     

The Effect of cytoskeleton inhibitors on coccolith morphology in Coccolithus braarudii and Scyphosphaera apsteinii

The calcite platelets of coccolithophores (Haptophyta), the coccoliths, are among the most elaborate biomineral structures. How these unicellular algae accomplish the complex morphogenesis of coccoliths is still largely unknown. It has long been proposed that the cytoskeleton plays a central role in shaping the growing coccoliths. Previous studies have indicated that disruption of the microtubule network led to defects in coccolith morphogenesis in Emiliania huxleyi and Coccolithus braarudii. Disruption of the actin network also led to defects in coccolith morphology in E. huxleyi, but its impact on coccolith morphology in C. braarudii was unclear, as coccolith secretion was largely inhibited under the conditions used. A more detailed examination of the role of actin and microtubule networks is therefore required to address the wider role of the cytoskeleton in coccolith morphogenesis. In this study, we have examined coccolith morphology in C. braarudii and Scyphosphaera apsteinii following treatment with the microtubule inhibitors vinblastine and colchicine (S. apsteinii only) and the actin inhibitor cytochalasin B. We found that all cytoskeleton inhibitors induced coccolith malformations, strongly suggesting that both microtubules and actin filaments are instrumental in morphogenesis. By demonstrating the requirement for the microtubule and actin networks in coccolith morphogenesis in diverse species, our results suggest that both of these cytoskeletal elements are likely to play conserved roles in defining coccolith morphology.     

Response of calcareous nannofossils to the Paleocene–Eocene Thermal Maximum: Observations on composition,preservation and calcification in sediments from ODP Site 1263 (Walvis Ridge — SW Atlantic)

In this study we present the results of a detailed analysis on calcareous nannofossil assemblages from sediment cores of ODP Site 1263 (Southern East Atlantic, Walvis Ridge). This section represents one of the few complete deep-sea sections that document the Paleocene–Eocene Thermal Maximum (PETM) in the pelagic realm. The PETM transient event was characterized by a brief, but intense interval of global warming, a global negative carbon isotope excursion (CIE), and widespread dissolution of seafloor carbonate sediments. Paired analysis at polarizing light microscope (LM) and Scanning Electron Microscope (SEM) documents the different “behavior” of nannofossils through the different phases of the PETM, at the onset of CIE, within the CIE, and during the recovery interval. The presence of anomalous specimens and morphotypes within some nannofossil taxa, recorded during previous LM high resolution analyses, has been further investigated in selected samples at the SEM. Besides the known representatives of the CIE-PETM “excursion nanno-flora”, as Rhomboaster calcitrapa group and Discoaster anartios, the analysis revealed the presence of peculiar morphotypes of Fasciculithus and deformed specimens of Discoaster nobilis group, Discoaster mediosus and Discoaster multiradiatus that are considered related to the anomalous amount of CO₂ in the ocean-atmosphere system during the early phase of PETM. Comparative analyses were performed in few selected samples from other PETM sections located at different latitudes in the Atlantic and Pacific oceans. Although the anomalous geochemical conditions during the PETM-CIE interval seem to have had some influence on the nannofossil production, calcification and assemblage composition, it results that local productivity together with post depositional (diagenetic) conditions were additional important controlling factors on nannofossil assemblages. Preliminary data from Eocene Thermal Maximum 2 (ETM2 or Elmo) suggest that nannofossil malformations are not exclusive of the PETM, and are associated to other episodes of perturbation of the C cycle.     

Calcareous nannofossil biostratigraphy (Upper Bajocian-Lower Bathonian) of the Ravin du Bès section (Bas Auran, Subalpine Basin, SE France): Evolutionary trends of Watznaueria barnesiae and new findings of “Rucinolithus” morphotypes

A biostratigraphic study based on calcareous nannofossils was performed on the Ravin du Bès section (Bas Auran area, SE France). Semi-quantitative estimates of total nannofossil and single species abundances showed that calcareous nannofloras are rare to common and their preservation is poor to moderate. The following biohorizons were identified and calibrated to ammonite biozonation: the first occurrence (FO) of Watznaueria aff. W. communis, the FO of Pseudoconus enigma; the FO of “Rucinolithus”; the last occurrence (LO) of Carinolithus magharensis; the FO of Stephanolithion speciosum octum and the FO of Watznaueria barnesiae. These results confirm that calcareous nannofossils are good biostratigraphic markers for the Bajocian/Bathonian boundary interval. Moreover, the occurrence of P. enigma allows a direct calibration between Tethyan and Boreal nannofossil events and biozones. Morphometric analyses were performed on the W. communis-W. barnesiae group in order to quantify changes between the two taxa. The intermediate form W. aff. W. communis has been identified; it comprises coccoliths very similar to W. communis, but with central area length ≤ 0.9 μm. This study allowed the detailed reconstruction of the evolutionary trends from W. communis to W. barnesiae: the intermediate taxon W. aff. W. communis appears abruptly in the latest Bajocian and persists after the FO of W. barnesiae in the earliest Bathonian. Our results support the model of punctuated equilibrium rather than a phyletic gradualism. The finding of “Rucinolithus” morphotypes, very similar to the Cretaceous taxa R. terebrodentarius and R. terebrodentarius youngii, is intriguing. They might represent nannoliths strongly affected by a recurrent “Lazarus effect” or be diagenetic artifacts or of bacterial bioprecipitation.     

INCOMPLETENESS OF THE COCCOSPHERE AS A POSSIBLE STIMULUS FOR COCCOLITH FORMATION IN PLEUROCHRYSIS CARTERAE(PRYMNESIOPHYCEAE)1

Pleurochrysis carterae is a marine biflagellate that produces calcified structures called coccoliths. The coccoliths are formed inside the cells and released from the latter after formation. The light dependence of calcium incorporation in this species was studied using⁴⁵Ca as a tracer. Cells exposed to a repeating cycle of 16 h of light and 8 h of darkness incorporated calcium in extracellular coccoliths at a more or less constant rate throughout a cycle. The cells divided during the dark periods with a concomitant decrease in size. Their size increased during the light periods Coccolith formation in cells incubated in continuous darkness was greatly reduced and finally ceased. These cells did not divide and did not increase in size. Removal of extracellular coccoliths prior to the calcium incorporation experiments stimulated coccolith formation both in dark-incubated cells and in cells exposed to a repeating light-dark cycle. Cells in the stationary phase of growth ceased producing coccoliths. Calcification could be induced in these cells by removal of the extracellular coccoliths. Based on these findings we suggest that cells of Pleurochrysis carterae tend to produce a complete cover of coccoliths and that the available cell surface is a factor controlling coccolith formation.     

INCOMPLETENESS OF THE COCCOSPHERE AS A POSSIBLE STIMULUS FOR COCCOLITH FORMATION IN PLEUROCHRYSIS CARTERAE (PRYMNESIOPHYCEAE)1

Pleurochrysis carterae is a marine biflagellate that produces calcified structures called coccoliths. The coccoliths are formed inside the cells and released from the latter after formation. The light dependence of calcium incorporation in this species was studied using ⁴⁵Ca as a tracer. Cells exposed to a repeating cycle of 16 h of light and 8 h of darkness incorporated calcium in extracellular coccoliths at a more or less constant rate throughout a cycle. The cells divided during the dark periods with a concomitant decrease in size. Their size increased during the light periods. Coccolith formation in cells incubated in continuous darkness was greatly reduced and finally ceased. These cells did not divide and did not increase in size. Removal of extracellular coccoliths prior to the calcium incorporation experiments stimulated coccolith formation both in dark-incubated cells and in cells exposed to a repeating light-dark cycle. Cells in the stationary phase of growth ceased producing coccoliths. Calcification could be induced in these cells by removal of the extracellular coccoliths. Based on these findings we suggest that cells of Pleurochrysis carterae tend to produce a complete cover of coccoliths and that the available cell surface is a factor controlling coccolith formation.     

Incorporation of zinc into the coccoliths of the microalga <Emphasis Type="Italic">Emiliania huxleyi</Emphasis>

The coccolithophore Emiliania huxleyi is covered with elaborated calcite plates, the so-called coccoliths, which are produced inside the cells. We investigated the incorporation of zinc into the coccoliths of E. huxleyi by applying different zinc and calcium amounts via the culture media and subsequently analyzing the zinc content in the cells and the Zn/Ca ratio of the coccoliths. To investigate the Zn/Ca ratio of coccoliths built in the manipulated media, the algae have first to be decalcified, i.e. coccolith free. We used a newly developed decalcification method to obtain ‘naked’ cells for cultivation. E. huxleyi proliferated and produced new coccoliths in all media with manipulated Zn/Ca ratios. The cells and the newly built coccoliths were investigated regarding their zinc content and their Zn/Ca ratio, respectively. High zinc amounts were taken up by the algae. The Zn/Ca ratio of the coccoliths was positively correlated to the Zn/Ca ratio of the applied media. The unique feature of the coccoliths was maintained also at high Zn/Ca ratios. We suggest the following pathway of the zinc ions into the coccoliths: first, the zinc ions are bound to the cell surface, followed by their transportation into the cytoplasm. Obviously, the zinc ions are removed afterwards into the coccolith vesicle, where the zinc is incorporated into the calcite coccoliths which are then extruded. The incorporation of toxic zinc ions into the coccoliths possibly due to a new function of the coccoliths as detoxification sites is discussed.     

Calcareous nannofossil biostratigraphy and paleoecology of the Cretaceous–Tertiary transition in the central eastern desert of Egypt

The Gebel Qreiya and nearby Wadi Hamama sections of the central Eastern Desert are among the most complete K/T boundary sequences known from Egypt. The two sections were analyzed spanning an interval from l.83 Myr below to about 3 Myr above the K/T boundary. A 1-cm-thick red clay layer at the K/T boundary at Gebel Qreiya contains an Ir anomaly of 5.4 ppb. The high-resolution study and well-preserved nannoflora provide good age control and the first quantitative records of calcareous nannofossil assemblages for paleoecological interpretations across the K/T transition in Egypt. Four zones (Micula murus, Micula prinsii, NP1, and NP2) were distinguished and correlated with other nannofossil and planktonic foraminiferal zonations that are broadly applicable for the eastern Tethys region. Latest Maastrichtian assemblages are abundant and diverse, though Cretaceous species richness progressively decreased across the K/T boundary. Dominant species include Arkhangelskiella cymbiformis, Micula decussata and Watznaueria barnesae, with high abundance of dissolution-resistant M. decussata reflecting periods of high environmental stress. Thoracosphaera blooms mark the K/T boundary and are followed by an acme of the opportunistic survivor Braarudosphaera bigelowii, the first appearance of the new Tertiary species Cruciplacolithus primus, and an acme of Coccolithus cavus/pelagicus. These successive abundance peaks provide the basis for subdivision of the Early Danian Zones NP1 and NP2 into five subzones. Correlation of selected nannofossil taxa from the Egyptian sections with those from various onshore marine and deep-sea sections provides insights into their paleoenvironmental and paleoecological affinities.